Summary
This strain contains 24 protein-level mutations:
- 10 mutations in Spike protein: T19R, K77T, G142D, EF 156-157 deletion, R158G, L452R, T478K, D614G, P681R, D950N
- 3 mutations in N protein: D63G, R203M, D377Y
- 1 mutation in M protein: I82T
- 1 mutation in NS3 protein: S26L
- 2 mutations in NS7a protein: V82A, T120I
- 1 mutation in NS9b protein: T60A
- 1 mutation in NS9c protein: G50W
- 1 mutation in NSP2 protein: P129L
- 1 mutation in NSP3 protein: P822L
- 2 mutations in NSP4 protein: D217N, F375S
- 1 mutation in NSP12 protein: P323L
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
T19R
Reference MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFR
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Mutated MFVFLVLLPLVSSQCVNLRTRTQLPPAYTNSFTRGVYYPDKVFR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | LVSSQCVNLT | LVSSQCVNLR | 21195 | 40 |
| HLA-A*68:01 | VSSQCVNLT | VSSQCVNLR | 26411 | 105 |
| HLA-A*31:01 | VSSQCVNLT | VSSQCVNLR | 27046 | 148 |
| HLA-A*33:03 | LVSSQCVNLT | LVSSQCVNLR | 28488 | 204 |
| HLA-A*31:01 | LVSSQCVNLT | LVSSQCVNLR | 25660 | 206 |
| HLA-A*33:03 | VSSQCVNLT | VSSQCVNLR | 32767 | 419 |
| HLA-A*30:01 | TTRTQLPPAYT | RTRTQLPPAYT | 1134 | 40 |
| HLA-B*08:01 | NLTTRTQL | NLRTRTQL | 531 | 22 |
| HLA-A*30:01 | TTRTQLPPAYTNS | RTRTQLPPAYTNS | 2190 | 92 |
| HLA-A*30:01 | TTRTQLPP | RTRTQLPP | 1734 | 80 |
| HLA-A*30:01 | TTRTQLPPAY | RTRTQLPPAY | 263 | 14 |
| HLA-A*30:01 | TTRTQLPPAYTNSF | RTRTQLPPAYTNSF | 7770 | 425 |
| HLA-A*30:01 | TTRTQLPPAYTN | RTRTQLPPAYTN | 4274 | 240 |
| HLA-B*27:05 | TTRTQLPPAY | RTRTQLPPAY | 2516 | 186 |
| HLA-A*30:02 | TTRTQLPPA | RTRTQLPPA | 2315 | 385 |
| HLA-A*30:02 | TTRTQLPPAY | RTRTQLPPAY | 288 | 49 |
| HLA-A*30:01 | TTRTQLPPA | RTRTQLPPA | 9 | 2 |
| HLA-B*15:03 | TTRTQLPPAY | RTRTQLPPAY | 888 | 198 |
| HLA-A*30:01 | NLTTRTQLPPA | NLRTRTQLPPA | 857 | 208 |
| HLA-B*15:01 | TTRTQLPPAY | RTRTQLPPAY | 618 | 168 |
| HLA-A*30:01 | LTTRTQLPPA | LRTRTQLPPA | 82 | 24 |
K77T
Reference QDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIR
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Mutated QDLFLPFFSNVTWFHAIHVSGTNGTTRFDNPVLPFNDGVYFASTEKSNIIR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | HVSGTNGTK | HVSGTNGTT | 54 | 20731 |
| HLA-A*68:01 | HAIHVSGTNGTK | HAIHVSGTNGTT | 174 | 32178 |
| HLA-A*30:01 | TKRFDNPVL | TTRFDNPVL | 6581 | 140 |
| HLA-A*30:01 | GTKRFDNPV | GTTRFDNPV | 120 | 5168 |
| HLA-C*16:01 | TKRFDNPVL | TTRFDNPVL | 10141 | 273 |
| HLA-B*15:03 | TKRFDNPVL | TTRFDNPVL | 162 | 3746 |
| HLA-B*15:03 | TKRFDNPVLPF | TTRFDNPVLPF | 100 | 750 |
| HLA-B*27:05 | TKRFDNPVLPF | TTRFDNPVLPF | 476 | 1794 |
| HLA-B*27:05 | KRFDNPVLPFN | TRFDNPVLPFN | 455 | 1697 |
| HLA-A*68:02 | NGTKRFDNPV | NGTTRFDNPV | 249 | 70 |
| HLA-A*68:02 | GTKRFDNPV | GTTRFDNPV | 772 | 227 |
| HLA-A*30:01 | GTKRFDNPVL | GTTRFDNPVL | 352 | 973 |
| HLA-B*27:05 | KRFDNPVLPF | TRFDNPVLPF | 31 | 79 |
G142D
Reference LLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCT
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Mutated LLIVNNATNVVIKVCEFQFCNDPFLDVYYHKNNKSWMES‑‑GVYSSANNCT
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*03:01 | GVYYHKNNK | DVYYHKNNK | 27 | 2841 |
| HLA-A*30:01 | GVYYHKNNK | DVYYHKNNK | 51 | 3035 |
| HLA-A*11:01 | GVYYHKNNK | DVYYHKNNK | 51 | 1768 |
| HLA-A*03:02 | GVYYHKNNK | DVYYHKNNK | 119 | 3887 |
| HLA-A*31:01 | GVYYHKNNK | DVYYHKNNK | 375 | 5203 |
| HLA-A*68:01 | GVYYHKNNK | DVYYHKNNK | 363 | 28 |
| HLA-A*68:02 | FCNDPFLGV | FCNDPFLDV | 281 | 3555 |
| HLA-A*33:03 | GVYYHKNNK | DVYYHKNNK | 3253 | 287 |
| HLA-A*02:06 | FCNDPFLGV | FCNDPFLDV | 116 | 1132 |
| HLA-A*02:11 | FCNDPFLGV | FCNDPFLDV | 87 | 751 |
| HLA-A*02:06 | EFQFCNDPFLGV | EFQFCNDPFLDV | 87 | 554 |
| HLA-A*30:02 | CNDPFLGVY | CNDPFLDVY | 310 | 1959 |
| HLA-A*02:02 | FCNDPFLGV | FCNDPFLDV | 365 | 2187 |
| HLA-A*02:06 | FQFCNDPFLGVY | FQFCNDPFLDVY | 135 | 784 |
| HLA-A*30:02 | FCNDPFLGVY | FCNDPFLDVY | 408 | 1738 |
| HLA-A*02:11 | EFQFCNDPFLGV | EFQFCNDPFLDV | 334 | 1214 |
| HLA-A*02:02 | EFQFCNDPFLGV | EFQFCNDPFLDV | 477 | 1725 |
| HLA-A*02:11 | FQFCNDPFLGVY | FQFCNDPFLDVY | 488 | 1737 |
| HLA-A*02:06 | FQFCNDPFLGV | FQFCNDPFLDV | 7 | 21 |
| HLA-A*02:11 | FQFCNDPFLGV | FQFCNDPFLDV | 17 | 49 |
| HLA-A*02:01 | FQFCNDPFLGV | FQFCNDPFLDV | 39 | 111 |
| HLA-A*30:02 | FQFCNDPFLGVY | FQFCNDPFLDVY | 336 | 923 |
| HLA-A*02:02 | FQFCNDPFLGV | FQFCNDPFLDV | 45 | 122 |
| HLA-B*15:01 | FQFCNDPFLGVY | FQFCNDPFLDVY | 28 | 56 |
EF 156-157 deletion
Reference CEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGK
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Mutated CEFQFCNDPFLDVYYHKNNKSWMES‑‑GVYSSANNCTFEYVSQPFLMDLEGK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | WMESEFRVY | WMESGVY | 10 | - |
| HLA-B*45:01 | SEFRVYSSA | SGVYSSA | 10 | - |
| HLA-B*18:01 | MESEFRVY | MESGVY | 11 | - |
| HLA-B*50:01 | SEFRVYSSA | SGVYSSA | 14 | - |
| HLA-A*31:01 | KSWMESEFR | KSWMESG | 17 | - |
| HLA-B*40:06 | SEFRVYSSA | SGVYSSA | 21 | - |
| HLA-B*40:02 | SEFRVYSSA | SGVYSSA | 29 | - |
| HLA-B*45:01 | MESEFRVYS | MESGVYS | 54 | - |
| HLA-B*15:01 | WMESEFRVY | WMESGVY | 67 | - |
| HLA-C*03:02 | WMESEFRVY | WMESGVY | 80 | - |
| HLA-A*29:02 | WMESEFRVY | WMESGVY | 109 | - |
| HLA-B*15:03 | NKSWMESEF | NKSWMES | 126 | - |
| HLA-B*50:01 | MESEFRVYS | MESGVYS | 146 | - |
| HLA-A*02:11 | WMESEFRV | WMESGV | 156 | - |
| HLA-B*15:03 | MESEFRVY | MESGVY | 180 | - |
| HLA-B*15:03 | HKNNKSWMESEFRVY | HKNNKSWMESGVY | - | 224 |
| HLA-C*14:02 | YYHKNNKSWMESEF | - | 227 | - |
| HLA-A*02:11 | SWMESEFRV | SWMESGV | 262 | - |
| HLA-A*30:02 | HKNNKSWMESEFRVY | HKNNKSWMESGVY | - | 278 |
| HLA-A*11:01 | KSWMESEFR | KSWMESG | 279 | - |
| HLA-B*40:06 | MESEFRVYS | MESGVYS | 299 | - |
| HLA-B*18:01 | SEFRVYSSA | SGVYSSA | 303 | - |
| HLA-A*02:02 | WMESEFRV | WMESGV | 320 | - |
| HLA-B*35:01 | WMESEFRVY | WMESGVY | 336 | - |
| HLA-B*18:01 | MESEFRVYS | MESGVYS | 338 | - |
| HLA-A*30:02 | WMESEFRVY | WMESGVY | 342 | - |
| HLA-B*40:02 | MESEFRVYS | MESGVYS | 383 | - |
| HLA-C*14:02 | WMESEFRVY | WMESGVY | 387 | - |
| HLA-B*40:02 | SEFRVYSS | SGVYSS | 414 | - |
| HLA-B*15:03 | YHKNNKSWMESEFRVY | YHKNNKSWMESGVY | - | 418 |
| HLA-B*44:03 | MESEFRVY | MESGVY | 439 | - |
| HLA-A*23:01 | SWMESEFRV | SWMESGV | 458 | - |
| HLA-A*03:02 | KSWMESEFR | KSWMESG | 465 | - |
| HLA-B*58:01 | KSWMESEF | KSWMES | 465 | - |
| HLA-A*68:01 | NKSWMESEFR | NKSWMESG | 75 | 32767 |
| HLA-A*31:01 | KNNKSWMESEFR | KNNKSWMESG | 126 | 28860 |
| HLA-A*33:03 | NKSWMESEFR | NKSWMESG | 293 | 32767 |
| HLA-A*33:03 | NNKSWMESEFR | NNKSWMESG | 340 | 32240 |
| HLA-B*45:01 | SEFRVYSSAN | SGVYSSAN | 384 | 32767 |
| HLA-A*31:01 | NKSWMESEFR | NKSWMESG | 402 | 32767 |
| HLA-B*50:01 | SEFRVYSSAN | SGVYSSAN | 423 | 32767 |
| HLA-C*03:02 | KSWMESEFRVY | KSWMESGVY | 3215 | 55 |
| HLA-A*02:11 | KSWMESEFRV | KSWMESGV | 212 | 11798 |
| HLA-A*68:02 | ESEFRVYSSA | ESGVYSSA | 187 | 8686 |
| HLA-A*29:02 | SWMESEFRVY | SWMESGVY | 49 | 2195 |
| HLA-B*45:01 | ESEFRVYSSA | ESGVYSSA | 138 | 5746 |
| HLA-B*58:01 | KSWMESEFRV | KSWMESGV | 187 | 7739 |
| HLA-A*02:02 | WMESEFRVYS | WMESGVYS | 286 | 11441 |
| HLA-A*02:06 | KSWMESEFRV | KSWMESGV | 255 | 9279 |
| HLA-C*16:01 | KSWMESEFRVY | KSWMESGVY | 2679 | 93 |
| HLA-B*15:03 | KSWMESEFRVY | KSWMESGVY | 250 | 9 |
| HLA-A*02:02 | WMESEFRVYSSA | WMESGVYSSA | 1287 | 50 |
| HLA-B*57:01 | KSWMESEFRV | KSWMESGV | 461 | 9513 |
| HLA-B*50:01 | ESEFRVYSSA | ESGVYSSA | 314 | 6125 |
| HLA-B*45:01 | MESEFRVYSS | MESGVYSS | 143 | 2610 |
| HLA-A*30:02 | NKSWMESEFRVY | NKSWMESGVY | 1025 | 59 |
| HLA-B*15:03 | NKSWMESEFRVY | NKSWMESGVY | 1077 | 62 |
| HLA-B*15:01 | SWMESEFRVY | SWMESGVY | 308 | 4989 |
| HLA-B*15:01 | KSWMESEFRVY | KSWMESGVY | 888 | 55 |
| HLA-A*30:02 | KNNKSWMESEFRVY | KNNKSWMESGVY | 963 | 61 |
| HLA-A*30:02 | KSWMESEFRVY | KSWMESGVY | 152 | 10 |
| HLA-A*30:02 | KSWMESEFRVYS | KSWMESGVYS | 2363 | 159 |
| HLA-A*29:02 | FRVYSSANNCTFEY | GVYSSANNCTFEY | 677 | 56 |
| HLA-B*15:01 | EFRVYSSANNCTF | GVYSSANNCTF | 3364 | 310 |
| HLA-A*02:11 | WMESEFRVYSSA | WMESGVYSSA | 1678 | 173 |
| HLA-B*18:01 | MESEFRVYSSA | MESGVYSSA | 1791 | 207 |
| HLA-A*30:02 | NNKSWMESEFRVY | NNKSWMESGVY | 2424 | 283 |
| HLA-B*15:03 | SWMESEFRVY | SWMESGVY | 196 | 1469 |
| HLA-A*29:02 | KSWMESEFRVY | KSWMESGVY | 1402 | 193 |
| HLA-B*50:01 | MESEFRVYSSA | MESGVYSSA | 142 | 22 |
| HLA-A*30:02 | SWMESEFRVY | SWMESGVY | 183 | 974 |
| HLA-B*45:01 | MESEFRVYSSA | MESGVYSSA | 73 | 18 |
| HLA-B*50:01 | WMESEFRVYSSA | WMESGVYSSA | 600 | 156 |
| HLA-B*15:03 | EFRVYSSANNCTF | GVYSSANNCTF | 1751 | 469 |
| HLA-B*45:01 | WMESEFRVYSSA | WMESGVYSSA | 969 | 267 |
| HLA-B*15:03 | FRVYSSANNCTF | GVYSSANNCTF | 131 | 469 |
| HLA-B*40:06 | MESEFRVYSSA | MESGVYSSA | 248 | 72 |
| HLA-B*40:02 | MESEFRVYSSA | MESGVYSSA | 453 | 141 |
| HLA-A*30:01 | KSWMESEFRVYS | KSWMESGVYS | 1192 | 384 |
| HLA-B*58:01 | KSWMESEFRVY | KSWMESGVY | 167 | 59 |
| HLA-B*15:01 | FRVYSSANNCTF | GVYSSANNCTF | 796 | 310 |
| HLA-B*45:01 | SWMESEFRVYSSA | SWMESGVYSSA | 579 | 286 |
R158G
Reference FQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQ
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Mutated FQFCNDPFLDVYYHKNNKSWMES‑‑GVYSSANNCTFEYVSQPFLMDLEGKQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | WMESEFRVY | WMESGVY | 10 | - |
| HLA-B*45:01 | SEFRVYSSA | SGVYSSA | 10 | - |
| HLA-B*18:01 | MESEFRVY | MESGVY | 11 | - |
| HLA-B*50:01 | SEFRVYSSA | SGVYSSA | 14 | - |
| HLA-A*31:01 | KSWMESEFR | KSWMESG | 17 | - |
| HLA-B*40:06 | SEFRVYSSA | SGVYSSA | 21 | - |
| HLA-B*40:02 | SEFRVYSSA | SGVYSSA | 29 | - |
| HLA-B*45:01 | MESEFRVYS | MESGVYS | 54 | - |
| HLA-B*15:01 | WMESEFRVY | WMESGVY | 67 | - |
| HLA-C*03:02 | WMESEFRVY | WMESGVY | 80 | - |
| HLA-A*29:02 | WMESEFRVY | WMESGVY | 109 | - |
| HLA-B*50:01 | MESEFRVYS | MESGVYS | 146 | - |
| HLA-A*02:11 | WMESEFRV | WMESGV | 156 | - |
| HLA-B*15:03 | MESEFRVY | MESGVY | 180 | - |
| HLA-B*15:03 | HKNNKSWMESEFRVY | HKNNKSWMESGVY | - | 224 |
| HLA-A*02:11 | SWMESEFRV | SWMESGV | 262 | - |
| HLA-A*30:02 | HKNNKSWMESEFRVY | HKNNKSWMESGVY | - | 278 |
| HLA-A*11:01 | KSWMESEFR | KSWMESG | 279 | - |
| HLA-B*40:06 | MESEFRVYS | MESGVYS | 299 | - |
| HLA-B*18:01 | SEFRVYSSA | SGVYSSA | 303 | - |
| HLA-A*02:02 | WMESEFRV | WMESGV | 320 | - |
| HLA-B*35:01 | WMESEFRVY | WMESGVY | 336 | - |
| HLA-B*18:01 | MESEFRVYS | MESGVYS | 338 | - |
| HLA-A*30:02 | WMESEFRVY | WMESGVY | 342 | - |
| HLA-B*40:02 | MESEFRVYS | MESGVYS | 383 | - |
| HLA-C*14:02 | WMESEFRVY | WMESGVY | 387 | - |
| HLA-B*40:02 | SEFRVYSS | SGVYSS | 414 | - |
| HLA-B*15:03 | YHKNNKSWMESEFRVY | YHKNNKSWMESGVY | - | 418 |
| HLA-B*44:03 | MESEFRVY | MESGVY | 439 | - |
| HLA-A*23:01 | SWMESEFRV | SWMESGV | 458 | - |
| HLA-A*03:02 | KSWMESEFR | KSWMESG | 465 | - |
| HLA-A*68:01 | NKSWMESEFR | NKSWMESG | 75 | 32767 |
| HLA-A*31:01 | KNNKSWMESEFR | KNNKSWMESG | 126 | 28860 |
| HLA-A*33:03 | NKSWMESEFR | NKSWMESG | 293 | 32767 |
| HLA-A*33:03 | NNKSWMESEFR | NNKSWMESG | 340 | 32240 |
| HLA-B*45:01 | SEFRVYSSAN | SGVYSSAN | 384 | 32767 |
| HLA-A*31:01 | NKSWMESEFR | NKSWMESG | 402 | 32767 |
| HLA-B*50:01 | SEFRVYSSAN | SGVYSSAN | 423 | 32767 |
| HLA-C*03:02 | KSWMESEFRVY | KSWMESGVY | 3215 | 55 |
| HLA-A*02:11 | KSWMESEFRV | KSWMESGV | 212 | 11798 |
| HLA-A*68:02 | ESEFRVYSSA | ESGVYSSA | 187 | 8686 |
| HLA-A*29:02 | SWMESEFRVY | SWMESGVY | 49 | 2195 |
| HLA-B*45:01 | ESEFRVYSSA | ESGVYSSA | 138 | 5746 |
| HLA-B*58:01 | KSWMESEFRV | KSWMESGV | 187 | 7739 |
| HLA-A*02:02 | WMESEFRVYS | WMESGVYS | 286 | 11441 |
| HLA-A*02:06 | KSWMESEFRV | KSWMESGV | 255 | 9279 |
| HLA-A*32:01 | RVYSSANNCTF | GVYSSANNCTF | 80 | 2794 |
| HLA-C*16:01 | KSWMESEFRVY | KSWMESGVY | 2679 | 93 |
| HLA-B*15:03 | KSWMESEFRVY | KSWMESGVY | 250 | 9 |
| HLA-A*02:02 | WMESEFRVYSSA | WMESGVYSSA | 1287 | 50 |
| HLA-A*30:01 | RVYSSANNC | GVYSSANNC | 250 | 5186 |
| HLA-B*57:01 | KSWMESEFRV | KSWMESGV | 461 | 9513 |
| HLA-B*50:01 | ESEFRVYSSA | ESGVYSSA | 314 | 6125 |
| HLA-B*45:01 | MESEFRVYSS | MESGVYSS | 143 | 2610 |
| HLA-A*30:02 | NKSWMESEFRVY | NKSWMESGVY | 1025 | 59 |
| HLA-B*15:03 | NKSWMESEFRVY | NKSWMESGVY | 1077 | 62 |
| HLA-B*15:01 | SWMESEFRVY | SWMESGVY | 308 | 4989 |
| HLA-B*15:01 | KSWMESEFRVY | KSWMESGVY | 888 | 55 |
| HLA-A*30:02 | KNNKSWMESEFRVY | KNNKSWMESGVY | 963 | 61 |
| HLA-A*30:02 | KSWMESEFRVY | KSWMESGVY | 152 | 10 |
| HLA-A*30:02 | KSWMESEFRVYS | KSWMESGVYS | 2363 | 159 |
| HLA-A*29:02 | FRVYSSANNCTFEY | GVYSSANNCTFEY | 677 | 56 |
| HLA-B*15:03 | RVYSSANNCTF | GVYSSANNCTF | 41 | 469 |
| HLA-B*15:01 | EFRVYSSANNCTF | GVYSSANNCTF | 3364 | 310 |
| HLA-B*58:01 | RVYSSANNCTF | GVYSSANNCTF | 126 | 1311 |
| HLA-B*15:03 | RVYSSANNCTFEY | GVYSSANNCTFEY | 203 | 2038 |
| HLA-A*02:11 | WMESEFRVYSSA | WMESGVYSSA | 1678 | 173 |
| HLA-B*57:01 | RVYSSANNCTF | GVYSSANNCTF | 493 | 4436 |
| HLA-B*18:01 | MESEFRVYSSA | MESGVYSSA | 1791 | 207 |
| HLA-A*03:01 | RVYSSANNCTFEY | GVYSSANNCTFEY | 151 | 1294 |
| HLA-A*30:02 | NNKSWMESEFRVY | NNKSWMESGVY | 2424 | 283 |
| HLA-B*15:03 | SWMESEFRVY | SWMESGVY | 196 | 1469 |
| HLA-A*29:02 | KSWMESEFRVY | KSWMESGVY | 1402 | 193 |
| HLA-A*30:02 | RVYSSANNCTFEY | GVYSSANNCTFEY | 27 | 178 |
| HLA-B*50:01 | MESEFRVYSSA | MESGVYSSA | 142 | 22 |
| HLA-A*03:02 | RVYSSANNCTFEY | GVYSSANNCTFEY | 282 | 1658 |
| HLA-A*30:02 | SWMESEFRVY | SWMESGVY | 183 | 974 |
| HLA-B*15:01 | RVYSSANNCTFEY | GVYSSANNCTFEY | 182 | 840 |
| HLA-B*15:01 | RVYSSANNCTF | GVYSSANNCTF | 75 | 310 |
| HLA-B*45:01 | MESEFRVYSSA | MESGVYSSA | 73 | 18 |
| HLA-A*11:01 | RVYSSANNCTFEY | GVYSSANNCTFEY | 151 | 584 |
| HLA-B*50:01 | WMESEFRVYSSA | WMESGVYSSA | 600 | 156 |
| HLA-B*15:03 | EFRVYSSANNCTF | GVYSSANNCTF | 1751 | 469 |
| HLA-B*45:01 | WMESEFRVYSSA | WMESGVYSSA | 969 | 267 |
| HLA-B*15:03 | FRVYSSANNCTF | GVYSSANNCTF | 131 | 469 |
| HLA-B*40:06 | MESEFRVYSSA | MESGVYSSA | 248 | 72 |
| HLA-B*40:02 | MESEFRVYSSA | MESGVYSSA | 453 | 141 |
| HLA-A*30:01 | KSWMESEFRVYS | KSWMESGVYS | 1192 | 384 |
| HLA-B*58:01 | KSWMESEFRVY | KSWMESGVY | 167 | 59 |
| HLA-B*15:01 | FRVYSSANNCTF | GVYSSANNCTF | 796 | 310 |
| HLA-A*02:11 | RVYSSANNCTFEYV | GVYSSANNCTFEYV | 267 | 623 |
| HLA-A*02:06 | RVYSSANNCTFEYV | GVYSSANNCTFEYV | 274 | 619 |
| HLA-B*45:01 | SWMESEFRVYSSA | SWMESGVYSSA | 579 | 286 |
L452R
Reference DDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated DDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*31:01 | KVGGNYNYL | KVGGNYNYR | 5813 | 13 |
| HLA-A*02:11 | YLYRLFRKSNL | YRYRLFRKSNL | 82 | 22451 |
| HLA-A*68:01 | SKVGGNYNYL | SKVGGNYNYR | 24692 | 119 |
| HLA-B*27:05 | YLYRLFRK | YRYRLFRK | 9806 | 50 |
| HLA-A*02:11 | YLYRLFRKS | YRYRLFRKS | 179 | 28031 |
| HLA-A*02:02 | YLYRLFRKSNL | YRYRLFRKSNL | 188 | 23354 |
| HLA-A*02:11 | KVGGNYNYL | KVGGNYNYR | 111 | 13729 |
| HLA-B*27:05 | YLYRLFRKS | YRYRLFRKS | 13862 | 117 |
| HLA-A*33:03 | DSKVGGNYNYL | DSKVGGNYNYR | 30679 | 292 |
| HLA-B*27:05 | YLYRLFRKSNLK | YRYRLFRKSNLK | 2218 | 22 |
| HLA-A*31:01 | SKVGGNYNYL | SKVGGNYNYR | 21677 | 233 |
| HLA-A*03:01 | YLYRLFRK | YRYRLFRK | 204 | 18425 |
| HLA-A*68:01 | KVGGNYNYL | KVGGNYNYR | 25432 | 286 |
| HLA-A*33:03 | KVGGNYNYL | KVGGNYNYR | 22954 | 269 |
| HLA-A*11:01 | KVGGNYNYL | KVGGNYNYR | 16150 | 194 |
| HLA-A*02:02 | KVGGNYNYL | KVGGNYNYR | 279 | 23086 |
| HLA-A*68:01 | DSKVGGNYNYL | DSKVGGNYNYR | 28915 | 378 |
| HLA-A*03:02 | YLYRLFRK | YRYRLFRK | 327 | 22983 |
| HLA-A*02:01 | YLYRLFRKSNL | YRYRLFRKSNL | 471 | 28211 |
| HLA-A*33:03 | SKVGGNYNYL | SKVGGNYNYR | 27973 | 469 |
| HLA-A*03:02 | KVGGNYNYL | KVGGNYNYR | 12848 | 234 |
| HLA-A*02:06 | KVGGNYNYL | KVGGNYNYR | 452 | 18600 |
| HLA-B*27:05 | YLYRLFRKSNLKP | YRYRLFRKSNLKP | 13004 | 347 |
| HLA-B*27:05 | YLYRLFRKSNLKPF | YRYRLFRKSNLKPF | 7101 | 214 |
| HLA-B*27:05 | YLYRLFRKSNL | YRYRLFRKSNL | 1360 | 48 |
| HLA-B*15:01 | YLYRLFRKSNLKPF | YRYRLFRKSNLKPF | 476 | 9205 |
| HLA-B*08:01 | YLYRLFRKSNL | YRYRLFRKSNL | 160 | 1946 |
| HLA-A*30:01 | LYRLFRKSN | RYRLFRKSN | 1444 | 120 |
| HLA-A*30:01 | LYRLFRKSNL | RYRLFRKSNL | 1104 | 93 |
| HLA-A*02:11 | KVGGNYNYLYRL | KVGGNYNYRYRL | 274 | 3087 |
| HLA-A*30:01 | LYRLFRKSNLKP | RYRLFRKSNLKP | 3059 | 277 |
| HLA-A*30:01 | LYRLFRKSNLK | RYRLFRKSNLK | 192 | 21 |
| HLA-A*03:01 | LYRLFRKSNLKP | RYRLFRKSNLKP | 3564 | 430 |
| HLA-A*30:01 | NYLYRLFRK | NYRYRLFRK | 512 | 64 |
| HLA-B*27:05 | LYRLFRKSNL | RYRLFRKSNL | 932 | 123 |
| HLA-A*23:01 | GNYNYLYRLF | GNYNYRYRLF | 181 | 1119 |
| HLA-A*23:01 | NYNYLYRLF | NYNYRYRLF | 20 | 119 |
| HLA-A*23:01 | KVGGNYNYLYRLF | KVGGNYNYRYRLF | 410 | 2404 |
| HLA-A*23:01 | VGGNYNYLYRLF | VGGNYNYRYRLF | 222 | 1236 |
| HLA-A*29:02 | VGGNYNYLY | VGGNYNYRY | 94 | 518 |
| HLA-A*31:01 | LYRLFRKSNLK | RYRLFRKSNLK | 356 | 67 |
| HLA-A*30:01 | YLYRLFRKSNLK | YRYRLFRKSNLK | 1354 | 258 |
| HLA-A*24:02 | NYNYLYRLF | NYNYRYRLF | 21 | 108 |
| HLA-A*29:02 | KVGGNYNYLY | KVGGNYNYRY | 100 | 505 |
| HLA-A*24:02 | GNYNYLYRLF | GNYNYRYRLF | 325 | 1597 |
| HLA-A*01:01 | NLDSKVGGNYNYLY | NLDSKVGGNYNYRY | 193 | 909 |
| HLA-A*24:02 | VGGNYNYLYRLF | VGGNYNYRYRLF | 391 | 1596 |
| HLA-A*03:01 | LYRLFRKSNLK | RYRLFRKSNLK | 65 | 18 |
| HLA-A*03:02 | LYRLFRKSNLK | RYRLFRKSNLK | 242 | 72 |
| HLA-A*30:01 | YNYLYRLFRK | YNYRYRLFRK | 379 | 125 |
| HLA-A*11:01 | LYRLFRKSNLK | RYRLFRKSNLK | 952 | 401 |
| HLA-B*15:03 | SKVGGNYNYLY | SKVGGNYNYRY | 313 | 720 |
| HLA-A*30:02 | VGGNYNYLY | VGGNYNYRY | 45 | 93 |
T478K
Reference YRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGV
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated YRLFRKSNLKPFERDISTEIYQAGSKPCNGVEGFNCYFPLQSYGFQPTNGV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | STEIYQAGST | STEIYQAGSK | 30358 | 64 |
| HLA-A*68:01 | STEIYQAGST | STEIYQAGSK | 22142 | 119 |
| HLA-A*03:01 | STEIYQAGST | STEIYQAGSK | 32241 | 248 |
| HLA-A*03:02 | STEIYQAGST | STEIYQAGSK | 32767 | 414 |
| HLA-B*35:01 | TPCNGVEGF | KPCNGVEGF | 319 | 2329 |
| HLA-A*02:11 | YQAGSTPCNGV | YQAGSKPCNGV | 173 | 742 |
| HLA-A*02:01 | YQAGSTPCNGV | YQAGSKPCNGV | 420 | 1594 |
| HLA-A*02:06 | YQAGSTPCNGV | YQAGSKPCNGV | 39 | 144 |
| HLA-B*15:03 | YQAGSTPCNGVEGF | YQAGSKPCNGVEGF | 217 | 656 |
| HLA-B*50:01 | TEIYQAGSTP | TEIYQAGSKP | 464 | 1371 |
| HLA-A*02:02 | YQAGSTPCNGV | YQAGSKPCNGV | 107 | 225 |
| HLA-C*14:02 | IYQAGSTPC | IYQAGSKPC | 134 | 276 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
P681R
Reference VNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVA
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VNNSYECDIPIGAGICASYQTQTNSRRRARSVASQSIIAYTMSLGAENSVA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*07:02 | SPRRARSVA | SRRRARSVA | 4 | 4441 |
| HLA-B*42:01 | SPRRARSVA | SRRRARSVA | 8 | 6100 |
| HLA-B*07:02 | SPRRARSVASQSI | SRRRARSVASQSI | 17 | 12179 |
| HLA-B*07:02 | SPRRARSV | SRRRARSV | 24 | 14015 |
| HLA-B*07:02 | SPRRARSVAS | SRRRARSVAS | 37 | 16690 |
| HLA-A*31:01 | ASYQTQTNSP | ASYQTQTNSR | 32767 | 83 |
| HLA-B*07:02 | SPRRARSVASQSII | SRRRARSVASQSII | 55 | 18257 |
| HLA-A*31:01 | SYQTQTNSP | SYQTQTNSR | 28695 | 96 |
| HLA-B*42:01 | SPRRARSV | SRRRARSV | 38 | 11288 |
| HLA-B*42:01 | SPRRARSVASQSI | SRRRARSVASQSI | 48 | 14255 |
| HLA-A*33:03 | SYQTQTNSP | SYQTQTNSR | 32767 | 122 |
| HLA-A*11:01 | ASYQTQTNSP | ASYQTQTNSR | 28751 | 146 |
| HLA-B*07:02 | TNSPRRARSVA | TNSRRRARSVA | 125 | 19949 |
| HLA-A*68:01 | ASYQTQTNSP | ASYQTQTNSR | 32767 | 212 |
| HLA-B*42:01 | SPRRARSVAS | SRRRARSVAS | 158 | 19305 |
| HLA-B*07:02 | NSPRRARSVA | NSRRRARSVA | 69 | 6677 |
| HLA-B*07:02 | TQTNSPRRARSVA | TQTNSRRRARSVA | 270 | 18446 |
| HLA-B*42:01 | SPRRARSVASQSII | SRRRARSVASQSII | 321 | 21055 |
| HLA-B*07:02 | QTNSPRRARSVA | QTNSRRRARSVA | 249 | 14015 |
| HLA-B*42:01 | TNSPRRARSVA | TNSRRRARSVA | 437 | 20986 |
| HLA-B*42:01 | NSPRRARSVA | NSRRRARSVA | 217 | 7845 |
| HLA-A*30:01 | NSPRRARSV | NSRRRARSV | 7195 | 290 |
| HLA-B*08:01 | NSPRRARSV | NSRRRARSV | 881 | 45 |
| HLA-A*68:01 | TQTNSPRRAR | TQTNSRRRAR | 254 | 2467 |
| HLA-A*68:01 | QTNSPRRAR | QTNSRRRAR | 95 | 788 |
| HLA-B*27:05 | PRRARSVASQSI | RRRARSVASQSI | 3476 | 498 |
| HLA-B*08:01 | SPRRARSVA | SRRRARSVA | 372 | 1939 |
| HLA-A*68:01 | CASYQTQTNSPR | CASYQTQTNSRR | 142 | 592 |
| HLA-A*31:01 | YQTQTNSPR | YQTQTNSRR | 441 | 1618 |
| HLA-B*08:01 | SPRRARSV | SRRRARSV | 428 | 1510 |
| HLA-A*11:01 | ASYQTQTNSPR | ASYQTQTNSRR | 346 | 1046 |
| HLA-A*31:01 | ASYQTQTNSPR | ASYQTQTNSRR | 139 | 389 |
| HLA-A*68:01 | QTQTNSPRR | QTQTNSRRR | 119 | 315 |
| HLA-A*68:01 | YQTQTNSPRR | YQTQTNSRRR | 206 | 538 |
| HLA-A*31:01 | SYQTQTNSPR | SYQTQTNSRR | 62 | 153 |
| HLA-A*33:03 | YQTQTNSPRRAR | YQTQTNSRRRAR | 306 | 723 |
| HLA-A*33:03 | TQTNSPRRAR | TQTNSRRRAR | 195 | 455 |
| HLA-A*33:03 | SYQTQTNSPR | SYQTQTNSRR | 130 | 287 |
| HLA-A*31:01 | QTQTNSPRRAR | QTQTNSRRRAR | 394 | 846 |
| HLA-A*31:01 | SYQTQTNSPRRAR | SYQTQTNSRRRAR | 193 | 408 |
| HLA-A*31:01 | TQTNSPRRAR | TQTNSRRRAR | 144 | 295 |
D950N
Reference NQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated NQFNSAIGKIQDSLSSTASALGKLQNVVNQNAQALNTLVKQLSSNFGAISS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | ALGKLQDV | ALGKLQNV | 2627 | 327 |
| HLA-A*02:11 | SALGKLQDV | SALGKLQNV | 475 | 86 |
| HLA-A*02:06 | SALGKLQDV | SALGKLQNV | 258 | 48 |
| HLA-C*15:02 | SALGKLQDV | SALGKLQNV | 566 | 162 |
| HLA-A*02:11 | KLQDVVNQNA | KLQNVVNQNA | 56 | 185 |
| HLA-A*02:11 | KLQDVVNQNAQA | KLQNVVNQNAQA | 95 | 313 |
| HLA-A*02:02 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 81 | 266 |
| HLA-A*02:02 | KLQDVVNQNAQA | KLQNVVNQNAQA | 318 | 989 |
| HLA-A*68:02 | STASALGKLQDV | STASALGKLQNV | 442 | 147 |
| HLA-C*12:03 | SALGKLQDV | SALGKLQNV | 197 | 66 |
| HLA-A*02:02 | KLQDVVNQNA | KLQNVVNQNA | 129 | 382 |
| HLA-A*02:11 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 71 | 193 |
| HLA-A*02:11 | ALGKLQDVV | ALGKLQNVV | 103 | 44 |
| HLA-A*02:01 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 435 | 956 |
| HLA-C*16:01 | SALGKLQDV | SALGKLQNV | 160 | 76 |
N protein
D63G
Reference KQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated KQRRPQGLPNNTASWFTALTQHGKEGLKFPRGQGVPINTNSSPDDQIGYYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | DLKFPRGQGV | GLKFPRGQGV | 5707 | 66 |
| HLA-A*02:02 | DLKFPRGQGV | GLKFPRGQGV | 6781 | 153 |
| HLA-B*07:02 | DLKFPRGQGVPI | GLKFPRGQGVPI | 1121 | 69 |
| HLA-B*42:01 | DLKFPRGQGVPI | GLKFPRGQGVPI | 1217 | 325 |
| HLA-B*15:03 | TQHGKEDLKF | TQHGKEGLKF | 206 | 56 |
| HLA-B*15:01 | TQHGKEDLKF | TQHGKEGLKF | 533 | 155 |
R203M
Reference GGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLN
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GGSQASSRSSSRSRNSSRNSTPGSSMGTSPARMAGNGGDAALALLLLDRLN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | RNSTPGSSR | RNSTPGSSM | 16125 | 70 |
| HLA-A*31:01 | SSRNSTPGSSR | SSRNSTPGSSM | 127 | 22198 |
| HLA-A*31:01 | RNSTPGSSR | RNSTPGSSM | 123 | 18362 |
| HLA-A*68:01 | NSSRNSTPGSSR | NSSRNSTPGSSM | 270 | 29029 |
| HLA-B*15:03 | SRNSTPGSSR | SRNSTPGSSM | 25421 | 299 |
| HLA-C*16:01 | NSTPGSSR | NSTPGSSM | 29183 | 443 |
| HLA-A*11:01 | SSRGTSPAR | SSMGTSPAR | 1462 | 49 |
| HLA-A*68:01 | TPGSSRGTSPAR | TPGSSMGTSPAR | 2549 | 139 |
| HLA-C*03:03 | SSRGTSPARM | SSMGTSPARM | 3138 | 197 |
| HLA-C*03:04 | SSRGTSPARM | SSMGTSPARM | 3138 | 197 |
| HLA-A*30:01 | SSRGTSPARM | SSMGTSPARM | 186 | 2270 |
| HLA-A*68:01 | STPGSSRGTSPAR | STPGSSMGTSPAR | 860 | 74 |
| HLA-A*30:01 | SSRGTSPARMA | SSMGTSPARMA | 147 | 1705 |
| HLA-B*15:03 | SRGTSPARM | SMGTSPARM | 2669 | 248 |
| HLA-A*68:01 | SSRGTSPAR | SSMGTSPAR | 186 | 19 |
| HLA-A*30:01 | SSRGTSPAR | SSMGTSPAR | 36 | 350 |
| HLA-A*30:01 | SSRGTSPA | SSMGTSPA | 297 | 2503 |
| HLA-A*03:02 | SSRGTSPAR | SSMGTSPAR | 2510 | 340 |
| HLA-C*03:02 | SSRGTSPARM | SSMGTSPARM | 1244 | 176 |
| HLA-B*15:03 | SSRGTSPARM | SSMGTSPARM | 1402 | 217 |
| HLA-C*16:01 | SSRGTSPARM | SSMGTSPARM | 843 | 153 |
| HLA-A*30:01 | RGTSPARMAG | MGTSPARMAG | 226 | 1123 |
| HLA-A*30:01 | SSRGTSPARMAG | SSMGTSPARMAG | 449 | 2001 |
| HLA-A*68:01 | NSTPGSSRGTSPAR | NSTPGSSMGTSPAR | 1348 | 416 |
| HLA-A*68:01 | GSSRGTSPAR | GSSMGTSPAR | 615 | 219 |
| HLA-A*33:03 | STPGSSRGTSPAR | STPGSSMGTSPAR | 968 | 432 |
| HLA-A*11:01 | GSSRGTSPAR | GSSMGTSPAR | 505 | 251 |
D377Y
Reference LLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LLNKHIDAYKTFPPTEPKKDKKKKAYETQALPQRQKKQQTVTLLPAADLDD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*08:02 | KADETQAL | KAYETQAL | 84 | 11531 |
| HLA-C*05:01 | KADETQAL | KAYETQAL | 164 | 15121 |
| HLA-A*31:01 | ADETQALPQR | AYETQALPQR | 17747 | 476 |
| HLA-C*16:01 | KADETQAL | KAYETQAL | 1681 | 76 |
| HLA-C*03:03 | KADETQAL | KAYETQAL | 2621 | 200 |
| HLA-C*03:04 | KADETQAL | KAYETQAL | 2621 | 200 |
| HLA-A*31:01 | KADETQALPQR | KAYETQALPQR | 1771 | 140 |
| HLA-C*03:02 | KADETQAL | KAYETQAL | 4694 | 456 |
| HLA-A*03:01 | KADETQALPQRQK | KAYETQALPQRQK | 3113 | 316 |
| HLA-B*15:03 | KKADETQAL | KKAYETQAL | 48 | 18 |
| HLA-B*15:03 | KKKADETQAL | KKKAYETQAL | 560 | 253 |
M protein
I82T
Reference LWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LWPVTLACFVLAAVYRINWITGGIATAMACLVGLMWLSYFIASFRLFARTR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | NWITGGIAI | NWITGGIAT | 109 | 4410 |
| HLA-A*68:02 | IAMACLVGL | TAMACLVGL | 424 | 40 |
| HLA-B*27:05 | YRINWITGGIAI | YRINWITGGIAT | 85 | 885 |
| HLA-A*68:02 | AIAMACLVGL | ATAMACLVGL | 1414 | 203 |
| HLA-A*02:11 | WITGGIAIA | WITGGIATA | 788 | 138 |
| HLA-A*68:02 | IAMACLVGLM | TAMACLVGLM | 2691 | 495 |
| HLA-A*02:06 | WITGGIAIA | WITGGIATA | 477 | 117 |
| HLA-A*02:01 | AIAMACLVGL | ATAMACLVGL | 97 | 388 |
| HLA-A*02:06 | IAIAMACLV | IATAMACLV | 90 | 359 |
| HLA-A*02:11 | AIAMACLVGL | ATAMACLVGL | 53 | 208 |
| HLA-A*02:02 | WITGGIAIA | WITGGIATA | 333 | 88 |
| HLA-A*02:11 | IAIAMACLV | IATAMACLV | 140 | 524 |
| HLA-A*02:02 | AIAMACLVGL | ATAMACLVGL | 41 | 133 |
| HLA-B*58:01 | IAMACLVGL | TAMACLVGL | 423 | 1182 |
| HLA-A*02:11 | GIAIAMACL | GIATAMACL | 350 | 144 |
| HLA-A*68:02 | WITGGIAIA | WITGGIATA | 918 | 378 |
| HLA-B*58:01 | IAMACLVGLM | TAMACLVGLM | 479 | 1090 |
| HLA-C*12:03 | IAIAMACLV | IATAMACLV | 47 | 105 |
| HLA-A*02:02 | GIAIAMACL | GIATAMACL | 110 | 50 |
| HLA-B*57:01 | IAMACLVGLMW | TAMACLVGLMW | 65 | 141 |
| HLA-A*02:02 | GIAIAMACLV | GIATAMACLV | 84 | 39 |
| HLA-C*12:02 | IAIAMACLV | IATAMACLV | 336 | 715 |
| HLA-A*02:06 | GIAIAMACL | GIATAMACL | 662 | 317 |
| HLA-A*02:02 | GGIAIAMACL | GGIATAMACL | 596 | 288 |
| HLA-B*58:01 | IAMACLVGLMW | TAMACLVGLMW | 19 | 39 |
| HLA-A*68:02 | IAIAMACLV | IATAMACLV | 168 | 339 |
| HLA-A*02:01 | IAMACLVGL | TAMACLVGL | 122 | 246 |
| HLA-A*02:11 | IAMACLVGL | TAMACLVGL | 70 | 141 |
| HLA-B*57:01 | AIAMACLVGLMW | ATAMACLVGLMW | 538 | 268 |
NS3 protein
S26L
Reference MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVA
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated MDLFMRIFTIGTVTLKQGEIKDATPLDFVRATATIPIQASLPFGWLIVGVA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:01 | GEIKDATPS | GEIKDATPL | 3209 | 6 |
| HLA-B*40:01 | QGEIKDATPS | QGEIKDATPL | 19790 | 91 |
| HLA-B*40:01 | KQGEIKDATPS | KQGEIKDATPL | 24194 | 242 |
| HLA-B*40:02 | GEIKDATPS | GEIKDATPL | 890 | 18 |
| HLA-B*40:02 | QGEIKDATPS | QGEIKDATPL | 9115 | 250 |
| HLA-B*15:03 | KQGEIKDATPS | KQGEIKDATPL | 9550 | 310 |
| HLA-B*40:02 | KQGEIKDATPS | KQGEIKDATPL | 9835 | 345 |
| HLA-B*15:03 | GEIKDATPS | GEIKDATPL | 11520 | 472 |
| HLA-A*31:01 | KDATPSDFVR | KDATPLDFVR | 1003 | 448 |
NS7a protein
V82A
Reference TCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQELYSPIFLIVAAI
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TCFSTQFAFACPDGVKHVYQLRARSASPKLFIRQEEVQELYSPIFLIVAAI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*03:02 | SVSPKLFIR | SASPKLFIR | 308 | 1994 |
| HLA-C*14:02 | VYQLRARSV | VYQLRARSA | 26 | 165 |
| HLA-A*68:02 | HVYQLRARSV | HVYQLRARSA | 354 | 2232 |
| HLA-A*11:01 | SVSPKLFIR | SASPKLFIR | 75 | 415 |
| HLA-A*74:01 | SVSPKLFIR | SASPKLFIR | 316 | 1586 |
| HLA-A*02:11 | SVSPKLFIRQEEV | SASPKLFIRQEEV | 228 | 464 |
| HLA-B*27:05 | LRARSVSPKL | LRARSASPKL | 201 | 407 |
| HLA-A*31:01 | SVSPKLFIR | SASPKLFIR | 17 | 34 |
NS9b protein
T60A
Reference NNVGPKVYPIILRLGSPLSLNMARKTLNSLEDKAFQLTPIAVQMTKLATTE
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated NNVGPKVYPIILRLGSPLSLNMARKALNSLEDKAFQLTPIAVQMTKLATTE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | ARKTLNSLEDK | ARKALNSLEDK | 431 | 5010 |
| HLA-A*11:01 | KTLNSLEDK | KALNSLEDK | 46 | 453 |
| HLA-A*11:01 | RKTLNSLEDK | RKALNSLEDK | 177 | 1563 |
| HLA-A*03:02 | KTLNSLEDK | KALNSLEDK | 210 | 1483 |
| HLA-A*03:01 | KTLNSLEDK | KALNSLEDK | 432 | 2909 |
| HLA-B*57:01 | KTLNSLEDKAF | KALNSLEDKAF | 307 | 1579 |
| HLA-A*30:01 | KTLNSLEDK | KALNSLEDK | 232 | 752 |
| HLA-B*07:02 | SLNMARKTL | SLNMARKAL | 761 | 240 |
| HLA-B*15:03 | RKTLNSLEDKAF | RKALNSLEDKAF | 83 | 31 |
| HLA-B*08:01 | SLNMARKTL | SLNMARKAL | 454 | 182 |
| HLA-B*42:01 | SLNMARKTL | SLNMARKAL | 430 | 182 |
| HLA-B*07:02 | SPLSLNMARKTL | SPLSLNMARKAL | 30 | 13 |
| HLA-B*08:01 | LNMARKTL | LNMARKAL | 98 | 44 |
| HLA-B*58:01 | KTLNSLEDKAF | KALNSLEDKAF | 257 | 561 |
NS9c protein
G50W
Reference AAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||||
Mutated AAVKPLLVPHHVVATVQEIQLQAAVWELLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*44:02 | QEIQLQAAVG | QEIQLQAAVW | 1217 | 7 |
| HLA-B*44:03 | QEIQLQAAVG | QEIQLQAAVW | 1214 | 9 |
| HLA-B*57:01 | ATVQEIQLQAAVG | ATVQEIQLQAAVW | 26533 | 223 |
| HLA-B*58:01 | ATVQEIQLQAAVG | ATVQEIQLQAAVW | 20797 | 177 |
| HLA-A*02:01 | QLQAAVGEL | QLQAAVWEL | 1290 | 14 |
| HLA-A*02:06 | QLQAAVGEL | QLQAAVWEL | 2794 | 38 |
| HLA-B*44:02 | VQEIQLQAAVG | VQEIQLQAAVW | 21030 | 295 |
| HLA-B*44:03 | VQEIQLQAAVG | VQEIQLQAAVW | 20104 | 294 |
| HLA-B*58:01 | TVQEIQLQAAVG | TVQEIQLQAAVW | 25991 | 417 |
| HLA-A*02:11 | LQAAVGEL | LQAAVWEL | 10677 | 233 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVWEL | 338 | 8 |
| HLA-A*02:11 | QLQAAVGEL | QLQAAVWEL | 120 | 3 |
| HLA-A*02:11 | QEIQLQAAVGEL | QEIQLQAAVWEL | 6768 | 188 |
| HLA-A*02:01 | IQLQAAVGEL | IQLQAAVWEL | 1138 | 33 |
| HLA-A*02:11 | VQEIQLQAAVGEL | VQEIQLQAAVWEL | 4401 | 128 |
| HLA-B*44:03 | QEIQLQAAVGE | QEIQLQAAVWE | 13040 | 406 |
| HLA-B*44:02 | QEIQLQAAVGE | QEIQLQAAVWE | 13676 | 445 |
| HLA-A*02:11 | TVQEIQLQAAVGEL | TVQEIQLQAAVWEL | 10392 | 366 |
| HLA-A*23:01 | VGELLLLEW | VWELLLLEW | 12678 | 450 |
| HLA-A*02:11 | EIQLQAAVGEL | EIQLQAAVWEL | 8016 | 329 |
| HLA-A*32:01 | QLQAAVGEL | QLQAAVWEL | 9675 | 426 |
| HLA-A*02:06 | LQAAVGEL | LQAAVWEL | 4676 | 218 |
| HLA-B*58:01 | VGELLLLEW | VWELLLLEW | 330 | 6053 |
| HLA-A*02:11 | AVGELLLLEWL | AVWELLLLEWL | 3883 | 254 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVWELL | 303 | 23 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVWEL | 92 | 8 |
| HLA-A*02:06 | IQLQAAVGEL | IQLQAAVWEL | 280 | 28 |
| HLA-A*02:02 | LQAAVGEL | LQAAVWEL | 3894 | 402 |
| HLA-A*02:11 | IQLQAAVGELL | IQLQAAVWELL | 3459 | 401 |
| HLA-A*02:01 | QLQAAVGELL | QLQAAVWELL | 961 | 127 |
| HLA-A*02:06 | AVGELLLLEWL | AVWELLLLEWL | 3303 | 475 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVWEL | 170 | 25 |
| HLA-A*02:11 | QLQAAVGELLL | QLQAAVWELLL | 733 | 108 |
| HLA-A*02:11 | LQAAVGELL | LQAAVWELL | 386 | 69 |
| HLA-B*58:01 | QAAVGELLL | QAAVWELLL | 1295 | 280 |
| HLA-A*02:01 | LQAAVGELL | LQAAVWELL | 938 | 204 |
| HLA-A*02:11 | AVGELLLLEWLA | AVWELLLLEWLA | 837 | 192 |
| HLA-A*02:02 | VQEIQLQAAVGEL | VQEIQLQAAVWEL | 1944 | 456 |
| HLA-C*12:03 | QAAVGELLL | QAAVWELLL | 1550 | 382 |
| HLA-A*02:06 | LQAAVGELL | LQAAVWELL | 65 | 17 |
| HLA-B*58:01 | AAVGELLLLEW | AAVWELLLLEW | 36 | 135 |
| HLA-A*02:11 | QLQAAVGELLLL | QLQAAVWELLLL | 563 | 151 |
| HLA-A*02:11 | LQAAVGELLL | LQAAVWELLL | 1331 | 368 |
| HLA-C*15:02 | QAAVGELLL | QAAVWELLL | 1474 | 409 |
| HLA-A*02:06 | AVGELLLLEWLA | AVWELLLLEWLA | 1441 | 409 |
| HLA-A*02:06 | QLQAAVGELL | QLQAAVWELL | 850 | 246 |
| HLA-B*44:03 | QEIQLQAAVGEL | QEIQLQAAVWEL | 1386 | 402 |
| HLA-A*02:01 | QLQAAVGELLL | QLQAAVWELLL | 1401 | 435 |
| HLA-B*57:01 | AAVGELLLLEW | AAVWELLLLEW | 138 | 418 |
| HLA-B*40:02 | QEIQLQAAVGEL | QEIQLQAAVWEL | 334 | 111 |
| HLA-B*15:01 | LQAAVGELLL | LQAAVWELLL | 109 | 275 |
| HLA-A*02:06 | LQAAVGELLL | LQAAVWELLL | 162 | 73 |
| HLA-A*02:02 | LQAAVGELL | LQAAVWELL | 44 | 20 |
| HLA-A*02:01 | LQAAVGELLL | LQAAVWELLL | 917 | 423 |
| HLA-B*44:02 | GELLLLEWL | WELLLLEWL | 497 | 1059 |
| HLA-B*44:03 | GELLLLEWL | WELLLLEWL | 430 | 910 |
| HLA-A*02:02 | QLQAAVGELLL | QLQAAVWELLL | 530 | 253 |
| HLA-C*16:01 | QAAVGELLL | QAAVWELLL | 263 | 127 |
| HLA-C*03:02 | QAAVGELLL | QAAVWELLL | 898 | 443 |
NSP2 protein
P129L
Reference IQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated IQPRVEKKKLDGFMGRIRSVYPVASLNECNQMCLSTLMKCDHCGETSWQTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | SPNECNQMCL | SLNECNQMCL | 19740 | 19 |
| HLA-C*03:03 | RSVYPVASP | RSVYPVASL | 15306 | 18 |
| HLA-C*03:04 | RSVYPVASP | RSVYPVASL | 15306 | 18 |
| HLA-A*02:11 | SPNECNQMCL | SLNECNQMCL | 21871 | 30 |
| HLA-A*02:02 | SPNECNQMCLSTL | SLNECNQMCLSTL | 19590 | 49 |
| HLA-C*16:01 | RSVYPVASP | RSVYPVASL | 10332 | 34 |
| HLA-A*02:11 | SPNECNQMCLSTL | SLNECNQMCLSTL | 27061 | 125 |
| HLA-A*02:01 | SPNECNQMCL | SLNECNQMCL | 27445 | 138 |
| HLA-A*02:11 | SPNECNQMC | SLNECNQMC | 32361 | 165 |
| HLA-C*03:02 | RSVYPVASP | RSVYPVASL | 11535 | 61 |
| HLA-C*15:02 | RSVYPVASP | RSVYPVASL | 8085 | 50 |
| HLA-A*32:01 | RSVYPVASP | RSVYPVASL | 9530 | 67 |
| HLA-C*12:03 | RSVYPVASP | RSVYPVASL | 12480 | 91 |
| HLA-B*42:01 | SPNECNQMCLSTL | SLNECNQMCLSTL | 140 | 16820 |
| HLA-B*42:01 | SPNECNQMCL | SLNECNQMCL | 90 | 10115 |
| HLA-B*07:02 | SPNECNQMCLSTL | SLNECNQMCLSTL | 212 | 22140 |
| HLA-B*07:02 | RIRSVYPVASP | RIRSVYPVASL | 14747 | 146 |
| HLA-C*17:01 | RSVYPVASP | RSVYPVASL | 19864 | 210 |
| HLA-A*02:02 | SPNECNQMC | SLNECNQMC | 32767 | 353 |
| HLA-B*07:02 | SPNECNQMCL | SLNECNQMCL | 242 | 17296 |
| HLA-B*15:03 | RSVYPVASP | RSVYPVASL | 5293 | 84 |
| HLA-B*42:01 | SPNECNQMCLSTLM | SLNECNQMCLSTLM | 330 | 18932 |
| HLA-B*08:01 | MGRIRSVYPVASP | MGRIRSVYPVASL | 8764 | 160 |
| HLA-C*12:02 | RSVYPVASP | RSVYPVASL | 16109 | 304 |
| HLA-B*27:05 | GRIRSVYPVASP | GRIRSVYPVASL | 4012 | 77 |
| HLA-B*42:01 | RIRSVYPVASP | RIRSVYPVASL | 20058 | 415 |
| HLA-C*14:02 | RSVYPVASP | RSVYPVASL | 22072 | 473 |
| HLA-B*07:02 | RSVYPVASP | RSVYPVASL | 19612 | 452 |
| HLA-B*15:03 | PNECNQMCLSTLM | LNECNQMCLSTLM | 9595 | 269 |
| HLA-B*15:01 | PNECNQMCLSTLM | LNECNQMCLSTLM | 13027 | 438 |
| HLA-B*15:01 | RSVYPVASP | RSVYPVASL | 9843 | 359 |
| HLA-A*02:02 | FMGRIRSVYPVASP | FMGRIRSVYPVASL | 3175 | 135 |
| HLA-B*58:01 | RSVYPVASP | RSVYPVASL | 2687 | 123 |
| HLA-B*57:01 | RSVYPVASP | RSVYPVASL | 6826 | 429 |
| HLA-A*02:11 | FMGRIRSVYPVASP | FMGRIRSVYPVASL | 1574 | 127 |
| HLA-A*02:02 | SPNECNQMCLSTLM | SLNECNQMCLSTLM | 4079 | 383 |
| HLA-A*30:01 | RSVYPVASP | RSVYPVASL | 759 | 162 |
| HLA-A*30:01 | RIRSVYPVASP | RIRSVYPVASL | 194 | 64 |
NSP3 protein
P822L
Reference GKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLT
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GKTFYVLPNDDTLRVEAFEYYHTTDLSFLGRYMSALNHTKKWKYPQVNGLT
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:01 | FEYYHTTDP | FEYYHTTDL | 3182 | 17 |
| HLA-B*15:03 | FEYYHTTDP | FEYYHTTDL | 5699 | 46 |
| HLA-C*14:02 | AFEYYHTTDP | AFEYYHTTDL | 26353 | 238 |
| HLA-B*42:01 | TDPSFLGRYM | TDLSFLGRYM | 162 | 14224 |
| HLA-C*16:01 | PSFLGRYM | LSFLGRYM | 6594 | 77 |
| HLA-B*40:01 | AFEYYHTTDP | AFEYYHTTDL | 13576 | 171 |
| HLA-B*07:02 | TDPSFLGRYM | TDLSFLGRYM | 285 | 20915 |
| HLA-C*14:02 | EYYHTTDP | EYYHTTDL | 20835 | 358 |
| HLA-B*40:02 | FEYYHTTDP | FEYYHTTDL | 1325 | 23 |
| HLA-C*12:03 | PSFLGRYM | LSFLGRYM | 14018 | 311 |
| HLA-B*42:01 | YHTTDPSFLGRYM | YHTTDLSFLGRYM | 475 | 20814 |
| HLA-C*03:02 | PSFLGRYM | LSFLGRYM | 16512 | 381 |
| HLA-B*40:02 | AFEYYHTTDP | AFEYYHTTDL | 8005 | 204 |
| HLA-B*42:01 | HTTDPSFLGRYM | HTTDLSFLGRYM | 464 | 17387 |
| HLA-B*18:01 | FEYYHTTDP | FEYYHTTDL | 3031 | 162 |
| HLA-A*30:01 | PSFLGRYMSA | LSFLGRYMSA | 2394 | 176 |
| HLA-B*50:01 | FEYYHTTDP | FEYYHTTDL | 73 | 377 |
| HLA-A*29:02 | YYHTTDPSF | YYHTTDLSF | 602 | 121 |
| HLA-A*29:02 | HTTDPSFLGRY | HTTDLSFLGRY | 362 | 81 |
| HLA-C*05:01 | TTDPSFLGRYM | TTDLSFLGRYM | 410 | 1751 |
| HLA-A*23:01 | FEYYHTTDPSF | FEYYHTTDLSF | 668 | 160 |
| HLA-A*29:02 | EYYHTTDPSF | EYYHTTDLSF | 1528 | 381 |
| HLA-A*24:02 | FEYYHTTDPSF | FEYYHTTDLSF | 1012 | 277 |
| HLA-A*23:01 | EYYHTTDPSF | EYYHTTDLSF | 105 | 30 |
| HLA-A*26:01 | TTDPSFLGRY | TTDLSFLGRY | 205 | 691 |
| HLA-A*29:02 | YYHTTDPSFLGRY | YYHTTDLSFLGRY | 339 | 103 |
| HLA-A*24:02 | EYYHTTDPSF | EYYHTTDLSF | 156 | 50 |
| HLA-A*23:01 | YYHTTDPSFL | YYHTTDLSFL | 395 | 143 |
| HLA-B*15:03 | YHTTDPSF | YHTTDLSF | 1219 | 446 |
| HLA-A*23:01 | YYHTTDPSF | YYHTTDLSF | 30 | 11 |
| HLA-A*24:02 | YYHTTDPSF | YYHTTDLSF | 34 | 13 |
| HLA-B*50:01 | FEYYHTTDPS | FEYYHTTDLS | 286 | 698 |
| HLA-C*03:02 | YYHTTDPSF | YYHTTDLSF | 347 | 147 |
| HLA-B*15:03 | YYHTTDPSF | YYHTTDLSF | 77 | 34 |
| HLA-A*24:02 | YYHTTDPSFL | YYHTTDLSFL | 379 | 173 |
| HLA-C*07:01 | YYHTTDPSF | YYHTTDLSF | 924 | 445 |
| HLA-C*16:01 | YYHTTDPSF | YYHTTDLSF | 801 | 397 |
| HLA-C*14:02 | EYYHTTDPSF | EYYHTTDLSF | 70 | 35 |
| HLA-A*30:02 | TTDPSFLGRY | TTDLSFLGRY | 104 | 52 |
NSP4 protein
D217N
Reference VLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWV
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VLMDGSIIQFPNTYLEGSVRVVTTFNSEYCRHGTCERSEAGVCVSTSGRWV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | SVRVVTTFD | SVRVVTTFN | 276 | 44 |
| HLA-C*16:01 | VVTTFDSEY | VVTTFNSEY | 381 | 152 |
| HLA-A*31:01 | TTFDSEYCR | TTFNSEYCR | 52 | 21 |
| HLA-A*31:01 | VTTFDSEYCR | VTTFNSEYCR | 192 | 82 |
| HLA-C*12:03 | VVTTFDSEY | VVTTFNSEY | 881 | 380 |
| HLA-A*31:01 | VVTTFDSEYCR | VVTTFNSEYCR | 398 | 174 |
F375S
Reference TFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TFYLTNDVSFLAHIQWMVMFTPLVPSWITIAYIICISTKHFYWFFSNYLKR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*32:01 | VMFTPLVPF | VMFTPLVPS | 16 | 6055 |
| HLA-B*15:03 | MVMFTPLVPF | MVMFTPLVPS | 5 | 1507 |
| HLA-B*15:01 | MVMFTPLVPF | MVMFTPLVPS | 6 | 1738 |
| HLA-A*32:01 | MVMFTPLVPF | MVMFTPLVPS | 29 | 7292 |
| HLA-B*15:03 | IQWMVMFTPLVPF | IQWMVMFTPLVPS | 27 | 6363 |
| HLA-B*15:01 | VMFTPLVPF | VMFTPLVPS | 9 | 2077 |
| HLA-A*23:01 | VMFTPLVPF | VMFTPLVPS | 134 | 24335 |
| HLA-B*15:03 | VMFTPLVPF | VMFTPLVPS | 2 | 342 |
| HLA-B*35:01 | MVMFTPLVPF | MVMFTPLVPS | 40 | 6791 |
| HLA-C*03:02 | MVMFTPLVPF | MVMFTPLVPS | 33 | 5557 |
| HLA-C*03:02 | VMFTPLVPF | VMFTPLVPS | 37 | 5797 |
| HLA-B*15:03 | WMVMFTPLVPF | WMVMFTPLVPS | 54 | 8157 |
| HLA-A*23:01 | MVMFTPLVPF | MVMFTPLVPS | 189 | 24188 |
| HLA-C*14:02 | MFTPLVPF | MFTPLVPS | 49 | 5985 |
| HLA-B*15:01 | IQWMVMFTPLVPF | IQWMVMFTPLVPS | 110 | 13230 |
| HLA-B*15:03 | QWMVMFTPLVPF | QWMVMFTPLVPS | 128 | 14472 |
| HLA-B*15:03 | HIQWMVMFTPLVPF | HIQWMVMFTPLVPS | 153 | 16597 |
| HLA-B*15:03 | MFTPLVPF | MFTPLVPS | 175 | 16680 |
| HLA-B*15:01 | QWMVMFTPLVPF | QWMVMFTPLVPS | 158 | 13834 |
| HLA-B*15:01 | WMVMFTPLVPF | WMVMFTPLVPS | 123 | 10227 |
| HLA-B*53:01 | MVMFTPLVPF | MVMFTPLVPS | 165 | 13431 |
| HLA-B*15:01 | HIQWMVMFTPLVPF | HIQWMVMFTPLVPS | 228 | 16523 |
| HLA-A*29:02 | MVMFTPLVPF | MVMFTPLVPS | 67 | 3758 |
| HLA-B*58:01 | MVMFTPLVPF | MVMFTPLVPS | 102 | 5714 |
| HLA-B*35:01 | VMFTPLVPF | VMFTPLVPS | 356 | 16676 |
| HLA-A*23:01 | QWMVMFTPLVPF | QWMVMFTPLVPS | 245 | 11141 |
| HLA-C*14:02 | VMFTPLVPF | VMFTPLVPS | 169 | 6949 |
| HLA-C*03:03 | VMFTPLVPF | VMFTPLVPS | 292 | 11908 |
| HLA-C*03:04 | VMFTPLVPF | VMFTPLVPS | 292 | 11908 |
| HLA-A*29:02 | VMFTPLVPF | VMFTPLVPS | 317 | 9903 |
| HLA-C*16:01 | MVMFTPLVPF | MVMFTPLVPS | 342 | 10607 |
| HLA-C*14:02 | MVMFTPLVPF | MVMFTPLVPS | 357 | 10966 |
| HLA-C*16:01 | VMFTPLVPF | VMFTPLVPS | 378 | 9529 |
| HLA-C*12:02 | MVMFTPLVPF | MVMFTPLVPS | 299 | 7393 |
| HLA-C*12:02 | VMFTPLVPF | VMFTPLVPS | 433 | 9308 |
| HLA-B*42:01 | MVMFTPLVPF | MVMFTPLVPS | 497 | 8185 |
| HLA-C*12:03 | VMFTPLVPF | VMFTPLVPS | 498 | 6321 |
| HLA-A*24:02 | FWITIAYII | SWITIAYII | 250 | 35 |
| HLA-B*15:03 | MVMFTPLVPFW | MVMFTPLVPSW | 362 | 2119 |
| HLA-A*02:01 | FTPLVPFWI | FTPLVPSWI | 384 | 1950 |
| HLA-A*02:11 | FTPLVPFWI | FTPLVPSWI | 43 | 204 |
| HLA-A*23:01 | FWITIAYII | SWITIAYII | 72 | 16 |
| HLA-C*01:02 | FTPLVPFWI | FTPLVPSWI | 2043 | 461 |
| HLA-A*02:06 | FTPLVPFWI | FTPLVPSWI | 102 | 446 |
| HLA-A*68:02 | MVMFTPLVPF | MVMFTPLVPS | 496 | 115 |
| HLA-A*23:01 | MFTPLVPFWI | MFTPLVPSWI | 197 | 789 |
| HLA-B*07:02 | VPFWITIAYI | VPSWITIAYI | 792 | 202 |
| HLA-A*02:11 | PLVPFWITI | PLVPSWITI | 368 | 1164 |
| HLA-A*02:02 | VMFTPLVPF | VMFTPLVPS | 651 | 206 |
| HLA-A*02:02 | MVMFTPLVPF | MVMFTPLVPS | 429 | 146 |
| HLA-A*02:01 | TPLVPFWITI | TPLVPSWITI | 369 | 1075 |
| HLA-B*53:01 | FTPLVPFWITIAY | FTPLVPSWITIAY | 720 | 249 |
| HLA-A*02:11 | TPLVPFWITI | TPLVPSWITI | 114 | 324 |
| HLA-A*24:02 | MFTPLVPFWI | MFTPLVPSWI | 379 | 1077 |
| HLA-A*02:01 | VMFTPLVPF | VMFTPLVPS | 728 | 262 |
| HLA-A*02:11 | MVMFTPLVPF | MVMFTPLVPS | 341 | 124 |
| HLA-A*02:01 | MVMFTPLVPF | MVMFTPLVPS | 840 | 307 |
| HLA-A*02:02 | TPLVPFWITI | TPLVPSWITI | 297 | 757 |
| HLA-A*02:02 | FTPLVPFWI | FTPLVPSWI | 135 | 344 |
| HLA-A*02:11 | MVMFTPLVPFWI | MVMFTPLVPSWI | 128 | 326 |
| HLA-A*02:06 | TPLVPFWITI | TPLVPSWITI | 471 | 1180 |
| HLA-C*12:03 | FTPLVPFWI | FTPLVPSWI | 1086 | 441 |
| HLA-B*15:01 | VMFTPLVPFW | VMFTPLVPSW | 212 | 522 |
| HLA-B*15:03 | VMFTPLVPFW | VMFTPLVPSW | 96 | 234 |
| HLA-A*02:11 | VMFTPLVPF | VMFTPLVPS | 151 | 62 |
| HLA-C*14:02 | MFTPLVPFW | MFTPLVPSW | 649 | 269 |
| HLA-B*53:01 | LVPFWITIAY | LVPSWITIAY | 174 | 74 |
| HLA-B*53:01 | VPFWITIAY | VPSWITIAY | 85 | 37 |
| HLA-C*16:01 | VPFWITIAY | VPSWITIAY | 703 | 314 |
| HLA-A*02:06 | MVMFTPLVPF | MVMFTPLVPS | 252 | 117 |
| HLA-A*02:06 | VMFTPLVPF | VMFTPLVPS | 359 | 175 |
| HLA-B*15:01 | LVPFWITIAY | LVPSWITIAY | 169 | 83 |
| HLA-B*35:01 | FTPLVPFWITIAY | FTPLVPSWITIAY | 22 | 11 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
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Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |