Summary
This strain contains 26 protein-level mutations:
- 9 mutations in Spike protein: T95I, 144-145 YY->SN, R346K, E484K, N501Y, D614G, Q677H, P681H, D950N
- 2 mutations in N protein: T205I, Q241K
- 1 mutation in NS3 protein: Q57H
- 3 mutations in NS8 protein: T11K, P38S, S67F
- 1 mutation in NS9b protein: R32L
- 1 mutation in NS9c protein: L52F
- 3 mutations in NSP3 protein: T237A, T720I, E997K
- 1 mutation in NSP4 protein: T492I
- 1 mutation in NSP6 protein: Q160R
- 1 mutation in NSP12 protein: P323L
- 2 mutations in NSP13 protein: P419S, L581F
- 1 mutation in NSP14 protein: V125F
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
T95I
Reference VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIV
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Mutated VSGTNGTKRFDNPVLPFNDGVYFASIEKSNIIRGWIFGTTLDSKTQSLLIV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*57:01 | STEKSNIIRGW | SIEKSNIIRGW | 135 | 2014 |
| HLA-B*58:01 | STEKSNIIRGW | SIEKSNIIRGW | 183 | 2582 |
| HLA-B*42:01 | LPFNDGVYFAST | LPFNDGVYFASI | 4329 | 325 |
| HLA-B*53:01 | LPFNDGVYFAST | LPFNDGVYFASI | 6125 | 497 |
| HLA-C*12:03 | ASTEKSNII | ASIEKSNII | 2443 | 439 |
| HLA-A*31:01 | ASTEKSNIIR | ASIEKSNIIR | 1324 | 354 |
| HLA-C*03:03 | FASTEKSNII | FASIEKSNII | 1633 | 453 |
| HLA-C*03:04 | FASTEKSNII | FASIEKSNII | 1633 | 453 |
| HLA-A*68:01 | ASTEKSNIIR | ASIEKSNIIR | 1387 | 410 |
| HLA-C*12:03 | FASTEKSNII | FASIEKSNII | 902 | 268 |
| HLA-A*11:01 | ASTEKSNIIR | ASIEKSNIIR | 1555 | 495 |
| HLA-C*15:02 | ASTEKSNII | ASIEKSNII | 729 | 264 |
| HLA-B*44:02 | TEKSNIIRGW | IEKSNIIRGW | 20 | 55 |
| HLA-B*44:03 | TEKSNIIRGW | IEKSNIIRGW | 33 | 84 |
| HLA-C*16:01 | ASTEKSNII | ASIEKSNII | 603 | 239 |
| HLA-A*68:01 | STEKSNIIR | SIEKSNIIR | 186 | 458 |
| HLA-A*33:03 | YFASTEKSNIIR | YFASIEKSNIIR | 337 | 148 |
| HLA-A*11:01 | GVYFASTEKS | GVYFASIEKS | 648 | 298 |
| HLA-A*11:01 | DGVYFASTEK | DGVYFASIEK | 205 | 96 |
| HLA-B*58:01 | ASTEKSNIIRGW | ASIEKSNIIRGW | 245 | 122 |
144-145 YY->SN
Reference IVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEY
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Mutated IVNNATNVVIKVCEFQFCNDPFLGVSNHKNNKSWMESEFRVYSSANNCTFEY
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | YYHKNNKSWM | SNHKNNKSWM | 19 | 9477 |
| HLA-B*15:03 | FQFCNDPFLGVY | FQFCNDPFLGVS | 11 | 4009 |
| HLA-B*15:01 | FQFCNDPFLGVY | FQFCNDPFLGVS | 28 | 8965 |
| HLA-C*14:02 | YYHKNNKSW | SNHKNNKSW | 52 | 14588 |
| HLA-A*29:02 | QFCNDPFLGVYY | QFCNDPFLGVSN | 113 | 28056 |
| HLA-A*24:02 | YYHKNNKSW | SNHKNNKSW | 116 | 26669 |
| HLA-A*23:01 | YYHKNNKSW | SNHKNNKSW | 150 | 23574 |
| HLA-A*29:02 | FQFCNDPFLGVYY | FQFCNDPFLGVSN | 184 | 28257 |
| HLA-B*15:03 | FQFCNDPFLGVYY | FQFCNDPFLGVSN | 47 | 6994 |
| HLA-A*01:01 | CNDPFLGVYY | CNDPFLGVSN | 202 | 27140 |
| HLA-A*29:02 | QFCNDPFLGVY | QFCNDPFLGVS | 192 | 25647 |
| HLA-C*03:02 | FCNDPFLGVY | FCNDPFLGVS | 164 | 21177 |
| HLA-A*23:01 | VYYHKNNKSW | VSNHKNNKSW | 230 | 28289 |
| HLA-C*14:02 | YYHKNNKSWMESEF | SNHKNNKSWMESEF | 227 | 26319 |
| HLA-B*15:01 | FQFCNDPFLGVYY | FQFCNDPFLGVSN | 129 | 13148 |
| HLA-B*15:03 | EFQFCNDPFLGVY | EFQFCNDPFLGVS | 253 | 23686 |
| HLA-A*24:02 | VYYHKNNKSW | VSNHKNNKSW | 326 | 30370 |
| HLA-A*01:01 | CNDPFLGVY | CNDPFLGVS | 232 | 20042 |
| HLA-B*58:01 | VYYHKNNKSW | VSNHKNNKSW | 5591 | 71 |
| HLA-A*29:02 | CNDPFLGVYY | CNDPFLGVSN | 439 | 32767 |
| HLA-A*01:01 | FCNDPFLGVY | FCNDPFLGVS | 317 | 22509 |
| HLA-A*29:02 | FQFCNDPFLGVY | FQFCNDPFLGVS | 388 | 25787 |
| HLA-B*57:01 | VYYHKNNKSW | VSNHKNNKSW | 4718 | 79 |
| HLA-A*30:02 | FQFCNDPFLGVY | FQFCNDPFLGVS | 336 | 18481 |
| HLA-A*30:02 | FCNDPFLGVY | FCNDPFLGVS | 408 | 21487 |
| HLA-A*30:02 | CNDPFLGVY | CNDPFLGVS | 310 | 15987 |
| HLA-C*14:02 | VYYHKNNKSWM | VSNHKNNKSWM | 327 | 15977 |
| HLA-A*03:01 | GVYYHKNNK | GVSNHKNNK | 27 | 337 |
| HLA-A*03:01 | LGVYYHKNNK | LGVSNHKNNK | 131 | 1441 |
| HLA-C*14:02 | YHKNNKSWM | NHKNNKSWM | 392 | 3326 |
| HLA-A*30:01 | GVYYHKNNK | GVSNHKNNK | 51 | 326 |
| HLA-A*03:01 | FLGVYYHKNNK | FLGVSNHKNNK | 455 | 2887 |
| HLA-A*31:01 | GVYYHKNNK | GVSNHKNNK | 375 | 2377 |
| HLA-A*68:01 | GVYYHKNNK | GVSNHKNNK | 363 | 1976 |
| HLA-A*33:03 | DPFLGVYYHK | DPFLGVSNHK | 280 | 1466 |
| HLA-A*03:02 | GVYYHKNNK | GVSNHKNNK | 119 | 558 |
| HLA-A*11:01 | GVYYHKNNK | GVSNHKNNK | 51 | 225 |
| HLA-A*68:01 | LGVYYHKNNK | LGVSNHKNNK | 494 | 1972 |
| HLA-A*68:01 | DPFLGVYYHK | DPFLGVSNHK | 398 | 1196 |
R346K
Reference QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNS
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Mutated QPTESIVRFPNITNLCPFGEVFNATKFASVYAWNRKRISNCVADYSVLYNS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*27:05 | TRFASVYAWNRK | TKFASVYAWNRK | 127 | 9178 |
| HLA-B*27:05 | TRFASVYAWNR | TKFASVYAWNR | 253 | 10999 |
| HLA-B*27:05 | ATRFASVYAW | ATKFASVYAW | 325 | 10397 |
| HLA-B*27:05 | TRFASVYAW | TKFASVYAW | 301 | 8433 |
| HLA-B*15:03 | TRFASVYAW | TKFASVYAW | 224 | 50 |
| HLA-A*30:01 | ATRFASVY | ATKFASVY | 303 | 1031 |
| HLA-A*68:01 | FGEVFNATR | FGEVFNATK | 338 | 1140 |
| HLA-A*30:01 | VFNATRFASVYA | VFNATKFASVYA | 112 | 371 |
| HLA-A*30:01 | FNATRFASVYA | FNATKFASVYA | 316 | 1031 |
| HLA-A*68:01 | CPFGEVFNATR | CPFGEVFNATK | 457 | 1485 |
| HLA-A*30:01 | ATRFASVYAW | ATKFASVYAW | 100 | 320 |
| HLA-A*30:01 | NATRFASVYA | NATKFASVYA | 40 | 123 |
| HLA-A*30:01 | ATRFASVYAWNRK | ATKFASVYAWNRK | 45 | 128 |
| HLA-A*03:01 | ATRFASVYAWNRK | ATKFASVYAWNRK | 151 | 308 |
| HLA-A*30:01 | ATRFASVYA | ATKFASVYA | 3 | 6 |
E484K
Reference SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
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Mutated SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:02 | VEGFNCYFPL | VKGFNCYFPL | 31 | 7011 |
| HLA-B*40:01 | VEGFNCYFPL | VKGFNCYFPL | 76 | 15210 |
| HLA-A*11:01 | GSTPCNGVE | GSTPCNGVK | 32767 | 444 |
| HLA-A*68:02 | EGFNCYFPL | KGFNCYFPL | 117 | 3877 |
| HLA-A*30:01 | EGFNCYFPL | KGFNCYFPL | 5413 | 164 |
| HLA-A*32:01 | EGFNCYFPL | KGFNCYFPL | 6548 | 210 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQSY | 1806 | 72 |
| HLA-B*15:01 | GVEGFNCYF | GVKGFNCYF | 3125 | 132 |
| HLA-A*31:01 | EGFNCYFPL | KGFNCYFPL | 4227 | 226 |
| HLA-B*15:03 | EGFNCYFPL | KGFNCYFPL | 2980 | 212 |
| HLA-A*02:11 | EGFNCYFPL | KGFNCYFPL | 2508 | 259 |
| HLA-B*15:03 | GVEGFNCYF | GVKGFNCYF | 1657 | 199 |
| HLA-A*02:06 | EGFNCYFPL | KGFNCYFPL | 1266 | 169 |
| HLA-B*35:01 | TPCNGVEGF | TPCNGVKGF | 319 | 1754 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQSY | 1081 | 208 |
| HLA-B*35:01 | NGVEGFNCY | NGVKGFNCY | 346 | 1439 |
| HLA-C*16:01 | EGFNCYFPL | KGFNCYFPL | 1861 | 498 |
| HLA-B*15:03 | VEGFNCYFPL | VKGFNCYFPL | 943 | 280 |
N501Y
Reference GSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG
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Mutated GSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*29:02 | QSYGFQPTN | QSYGFQPTY | 26152 | 24 |
| HLA-A*29:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 19514 | 25 |
| HLA-B*15:01 | LQSYGFQPTN | LQSYGFQPTY | 6135 | 8 |
| HLA-B*35:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8715 | 15 |
| HLA-C*03:02 | QSYGFQPTN | QSYGFQPTY | 12903 | 23 |
| HLA-B*15:03 | LQSYGFQPTN | LQSYGFQPTY | 2045 | 4 |
| HLA-B*15:03 | QSYGFQPTN | QSYGFQPTY | 6543 | 13 |
| HLA-C*16:01 | QSYGFQPTN | QSYGFQPTY | 10868 | 27 |
| HLA-B*35:01 | QSYGFQPTN | QSYGFQPTY | 24615 | 71 |
| HLA-C*12:03 | QSYGFQPTN | QSYGFQPTY | 12696 | 38 |
| HLA-B*15:03 | PLQSYGFQPTN | PLQSYGFQPTY | 22802 | 80 |
| HLA-C*14:02 | TNGVGYQPY | TYGVGYQPY | 5468 | 21 |
| HLA-A*29:02 | LQSYGFQPTN | LQSYGFQPTY | 28458 | 133 |
| HLA-C*03:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 23840 | 113 |
| HLA-C*03:02 | LQSYGFQPTN | LQSYGFQPTY | 23421 | 125 |
| HLA-A*30:02 | LQSYGFQPTN | LQSYGFQPTY | 7508 | 42 |
| HLA-A*30:02 | QSYGFQPTN | QSYGFQPTY | 5297 | 30 |
| HLA-B*15:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 20159 | 137 |
| HLA-C*03:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 27639 | 198 |
| HLA-A*29:02 | CYFPLQSYGFQPTN | CYFPLQSYGFQPTY | 27182 | 196 |
| HLA-C*14:02 | SYGFQPTN | SYGFQPTY | 20230 | 162 |
| HLA-A*29:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 32767 | 264 |
| HLA-B*15:01 | QSYGFQPTN | QSYGFQPTY | 21529 | 195 |
| HLA-C*14:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18230 | 169 |
| HLA-B*15:03 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 22168 | 206 |
| HLA-A*11:01 | QSYGFQPTN | QSYGFQPTY | 22068 | 241 |
| HLA-C*16:01 | LQSYGFQPTN | LQSYGFQPTY | 27149 | 326 |
| HLA-C*12:02 | QSYGFQPTN | QSYGFQPTY | 21029 | 267 |
| HLA-B*15:01 | PLQSYGFQPTN | PLQSYGFQPTY | 32767 | 449 |
| HLA-B*35:01 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 28375 | 395 |
| HLA-C*12:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27893 | 431 |
| HLA-C*16:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27733 | 434 |
| HLA-C*02:02 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-C*02:10 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-A*30:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18743 | 310 |
| HLA-B*15:03 | LQSYGFQPTNG | LQSYGFQPTYG | 6387 | 109 |
| HLA-B*53:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8451 | 151 |
| HLA-C*12:03 | LQSYGFQPTN | LQSYGFQPTY | 26641 | 480 |
| HLA-A*32:01 | QSYGFQPTN | QSYGFQPTY | 16619 | 311 |
| HLA-A*30:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 21475 | 466 |
| HLA-C*03:02 | NGVGYQPY | YGVGYQPY | 10463 | 451 |
| HLA-B*57:01 | QSYGFQPTN | QSYGFQPTY | 4070 | 215 |
| HLA-B*58:01 | QSYGFQPTN | QSYGFQPTY | 1532 | 83 |
| HLA-A*02:11 | NGVGYQPYRV | YGVGYQPYRV | 5989 | 327 |
| HLA-B*57:01 | LQSYGFQPTN | LQSYGFQPTY | 3839 | 244 |
| HLA-B*58:01 | LQSYGFQPTN | LQSYGFQPTY | 1885 | 155 |
| HLA-B*15:03 | NGVGYQPY | YGVGYQPY | 4677 | 467 |
| HLA-A*31:01 | TNGVGYQPYR | TYGVGYQPYR | 811 | 84 |
| HLA-A*33:03 | TNGVGYQPYR | TYGVGYQPYR | 573 | 62 |
| HLA-A*29:02 | TNGVGYQPY | TYGVGYQPY | 2031 | 237 |
| HLA-A*29:02 | PTNGVGYQPY | PTYGVGYQPY | 1211 | 177 |
| HLA-A*02:11 | YGFQPTNGV | YGFQPTYGV | 307 | 49 |
| HLA-A*02:01 | YGFQPTNGV | YGFQPTYGV | 2160 | 459 |
| HLA-A*68:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1073 | 242 |
| HLA-A*02:11 | LQSYGFQPTNGV | LQSYGFQPTYGV | 1446 | 396 |
| HLA-A*31:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1377 | 406 |
| HLA-A*02:06 | YGFQPTNGV | YGFQPTYGV | 174 | 52 |
| HLA-A*33:03 | PTNGVGYQPYR | PTYGVGYQPYR | 1345 | 407 |
| HLA-A*02:02 | YGFQPTNGV | YGFQPTYGV | 868 | 287 |
| HLA-A*02:06 | QSYGFQPTNGV | QSYGFQPTYGV | 1121 | 407 |
| HLA-A*68:02 | YGFQPTNGV | YGFQPTYGV | 73 | 28 |
| HLA-A*68:02 | QSYGFQPTNGV | QSYGFQPTYGV | 122 | 48 |
| HLA-B*15:01 | GFQPTNGVGY | GFQPTYGVGY | 774 | 311 |
| HLA-A*29:02 | YGFQPTNGVGY | YGFQPTYGVGY | 1158 | 486 |
| HLA-A*31:01 | NGVGYQPYR | YGVGYQPYR | 438 | 185 |
| HLA-A*02:06 | LQSYGFQPTNGV | LQSYGFQPTYGV | 249 | 111 |
| HLA-A*30:02 | PTNGVGYQPY | PTYGVGYQPY | 465 | 210 |
| HLA-B*15:03 | FQPTNGVGY | FQPTYGVGY | 63 | 29 |
| HLA-A*02:02 | LQSYGFQPTNGV | LQSYGFQPTYGV | 594 | 280 |
| HLA-B*15:03 | GFQPTNGVGY | GFQPTYGVGY | 769 | 363 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
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Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
Q677H
Reference GAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAE
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Mutated GAEHVNNSYECDIPIGAGICASYQTHTNSHRRARSVASQSIIAYTMSLGAE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*07:02 | TQTNSPRRARSVA | THTNSHRRARSVA | 270 | 21705 |
| HLA-C*14:02 | SYQTQTNSP | SYQTHTNSH | 3691 | 54 |
| HLA-B*07:02 | QTNSPRRARSVA | HTNSHRRARSVA | 249 | 8263 |
| HLA-A*68:01 | YQTQTNSPRR | YQTHTNSHRR | 206 | 39 |
| HLA-A*68:01 | QTQTNSPRR | QTHTNSHRR | 119 | 24 |
| HLA-A*31:01 | ASYQTQTNSPRR | ASYQTHTNSHRR | 433 | 89 |
| HLA-A*30:01 | QTNSPRRAR | HTNSHRRAR | 1146 | 260 |
| HLA-A*33:03 | QTQTNSPRR | QTHTNSHRR | 445 | 133 |
| HLA-A*68:01 | TQTNSPRRAR | THTNSHRRAR | 254 | 713 |
| HLA-A*33:03 | YQTQTNSPRR | YQTHTNSHRR | 967 | 356 |
| HLA-A*11:01 | ASYQTQTNSPR | ASYQTHTNSHR | 346 | 890 |
| HLA-A*31:01 | QTQTNSPRR | QTHTNSHRR | 355 | 163 |
| HLA-A*31:01 | SYQTQTNSPRR | SYQTHTNSHRR | 437 | 207 |
| HLA-A*30:01 | QTNSPRRARSVA | HTNSHRRARSVA | 662 | 321 |
P681H
Reference VNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVA
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Mutated VNNSYECDIPIGAGICASYQTHTNSHRRARSVASQSIIAYTMSLGAENSVA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*07:02 | SPRRARSVA | SHRRARSVA | 4 | 4721 |
| HLA-B*42:01 | SPRRARSVA | SHRRARSVA | 8 | 6169 |
| HLA-B*07:02 | SPRRARSVASQSI | SHRRARSVASQSI | 17 | 11433 |
| HLA-B*07:02 | SPRRARSV | SHRRARSV | 24 | 12728 |
| HLA-B*07:02 | SPRRARSVAS | SHRRARSVAS | 37 | 14758 |
| HLA-B*07:02 | SPRRARSVASQSII | SHRRARSVASQSII | 55 | 17736 |
| HLA-B*42:01 | SPRRARSV | SHRRARSV | 38 | 11622 |
| HLA-B*42:01 | SPRRARSVASQSI | SHRRARSVASQSI | 48 | 14418 |
| HLA-B*07:02 | TNSPRRARSVA | TNSHRRARSVA | 125 | 20078 |
| HLA-B*42:01 | SPRRARSVAS | SHRRARSVAS | 158 | 17461 |
| HLA-B*07:02 | NSPRRARSVA | NSHRRARSVA | 69 | 7270 |
| HLA-B*07:02 | TQTNSPRRARSVA | THTNSHRRARSVA | 270 | 21705 |
| HLA-C*14:02 | SYQTQTNSP | SYQTHTNSH | 3691 | 54 |
| HLA-B*42:01 | SPRRARSVASQSII | SHRRARSVASQSII | 321 | 21521 |
| HLA-B*42:01 | TNSPRRARSVA | TNSHRRARSVA | 437 | 21599 |
| HLA-B*42:01 | NSPRRARSVA | NSHRRARSVA | 217 | 8330 |
| HLA-B*07:02 | QTNSPRRARSVA | HTNSHRRARSVA | 249 | 8263 |
| HLA-B*08:01 | NSPRRARSV | NSHRRARSV | 881 | 69 |
| HLA-B*08:01 | SPRRARSVA | SHRRARSVA | 372 | 3831 |
| HLA-B*08:01 | SPRRARSV | SHRRARSV | 428 | 2448 |
| HLA-A*68:01 | YQTQTNSPRR | YQTHTNSHRR | 206 | 39 |
| HLA-A*68:01 | QTQTNSPRR | QTHTNSHRR | 119 | 24 |
| HLA-A*31:01 | ASYQTQTNSPRR | ASYQTHTNSHRR | 433 | 89 |
| HLA-A*30:01 | QTNSPRRAR | HTNSHRRAR | 1146 | 260 |
| HLA-C*16:01 | NSPRRARSV | NSHRRARSV | 1247 | 314 |
| HLA-A*33:03 | QTQTNSPRR | QTHTNSHRR | 445 | 133 |
| HLA-C*12:03 | NSPRRARSV | NSHRRARSV | 1110 | 365 |
| HLA-A*68:01 | TQTNSPRRAR | THTNSHRRAR | 254 | 713 |
| HLA-A*33:03 | YQTQTNSPRR | YQTHTNSHRR | 967 | 356 |
| HLA-A*11:01 | ASYQTQTNSPR | ASYQTHTNSHR | 346 | 890 |
| HLA-A*31:01 | QTQTNSPRR | QTHTNSHRR | 355 | 163 |
| HLA-A*31:01 | SYQTQTNSPRR | SYQTHTNSHRR | 437 | 207 |
| HLA-A*30:01 | QTNSPRRARSVA | HTNSHRRARSVA | 662 | 321 |
D950N
Reference NQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated NQFNSAIGKIQDSLSSTASALGKLQNVVNQNAQALNTLVKQLSSNFGAISS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | ALGKLQDV | ALGKLQNV | 2627 | 327 |
| HLA-A*02:11 | SALGKLQDV | SALGKLQNV | 475 | 86 |
| HLA-A*02:06 | SALGKLQDV | SALGKLQNV | 258 | 48 |
| HLA-C*15:02 | SALGKLQDV | SALGKLQNV | 566 | 162 |
| HLA-A*02:11 | KLQDVVNQNA | KLQNVVNQNA | 56 | 185 |
| HLA-A*02:11 | KLQDVVNQNAQA | KLQNVVNQNAQA | 95 | 313 |
| HLA-A*02:02 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 81 | 266 |
| HLA-A*02:02 | KLQDVVNQNAQA | KLQNVVNQNAQA | 318 | 989 |
| HLA-A*68:02 | STASALGKLQDV | STASALGKLQNV | 442 | 147 |
| HLA-C*12:03 | SALGKLQDV | SALGKLQNV | 197 | 66 |
| HLA-A*02:02 | KLQDVVNQNA | KLQNVVNQNA | 129 | 382 |
| HLA-A*02:11 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 71 | 193 |
| HLA-A*02:11 | ALGKLQDVV | ALGKLQNVV | 103 | 44 |
| HLA-A*02:01 | KLQDVVNQNAQAL | KLQNVVNQNAQAL | 435 | 956 |
| HLA-C*16:01 | SALGKLQDV | SALGKLQNV | 160 | 76 |
N protein
T205I
Reference SQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated SQASSRSSSRSRNSSRNSTPGSSRGISPARMAGNGGDAALALLLLDRLNQL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*01:02 | STPGSSRGT | STPGSSRGI | 10810 | 468 |
| HLA-A*30:01 | GTSPARMAG | GISPARMAG | 493 | 1901 |
| HLA-A*68:01 | SSRGTSPAR | SSRGISPAR | 186 | 61 |
| HLA-A*68:01 | STPGSSRGTSPAR | STPGSSRGISPAR | 860 | 288 |
| HLA-A*30:01 | RGTSPARMA | RGISPARMA | 1160 | 422 |
| HLA-A*68:01 | GSSRGTSPAR | GSSRGISPAR | 615 | 248 |
| HLA-A*31:01 | GSSRGTSPAR | GSSRGISPAR | 262 | 111 |
| HLA-A*33:03 | SSRGTSPAR | SSRGISPAR | 106 | 46 |
| HLA-A*33:03 | STPGSSRGTSPAR | STPGSSRGISPAR | 968 | 473 |
Q241K
Reference DAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATK
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated DAALALLLLDRLNQLESKMSGKGQQKQGQTVTKKSAAEASKKPRQKRTATK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*03:01 | KMSGKGQQQ | KMSGKGQQK | 11204 | 32 |
| HLA-A*03:02 | KMSGKGQQQ | KMSGKGQQK | 14761 | 97 |
| HLA-A*03:01 | SKMSGKGQQQ | SKMSGKGQQK | 28438 | 299 |
| HLA-A*11:01 | QQQGQTVTK | QKQGQTVTK | 279 | 18561 |
| HLA-A*11:01 | KMSGKGQQQ | KMSGKGQQK | 27870 | 481 |
| HLA-A*30:01 | KMSGKGQQQ | KMSGKGQQK | 9030 | 203 |
| HLA-A*03:01 | QQGQTVTKK | KQGQTVTKK | 4266 | 478 |
NS3 protein
Q57H
Reference TATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCN
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TATIPIQASLPFGWLIVGVALLAVFHSASKIITLKKRWQLALSKGVHFVCN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:01 | FQSASKIITL | FHSASKIITL | 156 | 6196 |
| HLA-A*02:06 | FQSASKIITL | FHSASKIITL | 43 | 1441 |
| HLA-A*02:01 | FQSASKIITL | FHSASKIITL | 205 | 5629 |
| HLA-A*02:11 | FQSASKIITL | FHSASKIITL | 79 | 2010 |
| HLA-A*02:02 | FQSASKIITL | FHSASKIITL | 38 | 957 |
| HLA-B*38:01 | FQSASKIITL | FHSASKIITL | 2071 | 94 |
| HLA-B*15:03 | FQSASKIITL | FHSASKIITL | 88 | 851 |
| HLA-C*03:03 | QSASKIITL | HSASKIITL | 285 | 37 |
| HLA-C*03:04 | QSASKIITL | HSASKIITL | 285 | 37 |
| HLA-C*03:02 | QSASKIITL | HSASKIITL | 724 | 94 |
| HLA-C*12:03 | QSASKIITL | HSASKIITL | 250 | 34 |
| HLA-C*12:02 | QSASKIITL | HSASKIITL | 1730 | 284 |
| HLA-C*02:02 | QSASKIITL | HSASKIITL | 2653 | 440 |
| HLA-C*02:10 | QSASKIITL | HSASKIITL | 2653 | 440 |
| HLA-A*68:02 | QSASKIITL | HSASKIITL | 1085 | 232 |
| HLA-C*17:01 | QSASKIITL | HSASKIITL | 453 | 102 |
| HLA-A*30:01 | QSASKIITLK | HSASKIITLK | 727 | 164 |
| HLA-A*30:01 | LAVFQSASK | LAVFHSASK | 1169 | 264 |
| HLA-C*15:02 | QSASKIITL | HSASKIITL | 222 | 51 |
| HLA-C*16:01 | QSASKIITL | HSASKIITL | 52 | 12 |
| HLA-A*68:01 | QSASKIITLKK | HSASKIITLKK | 1356 | 322 |
| HLA-A*30:01 | QSASKIITLKK | HSASKIITLKK | 1813 | 486 |
| HLA-A*68:01 | QSASKIITLKKR | HSASKIITLKKR | 683 | 195 |
| HLA-A*68:01 | QSASKIITLK | HSASKIITLK | 35 | 10 |
| HLA-A*02:11 | LLAVFQSA | LLAVFHSA | 1121 | 356 |
| HLA-A*31:01 | QSASKIITLKKR | HSASKIITLKKR | 968 | 344 |
| HLA-A*03:01 | QSASKIITLK | HSASKIITLK | 214 | 83 |
| HLA-A*03:01 | AVFQSASKIITLKK | AVFHSASKIITLKK | 227 | 90 |
| HLA-A*02:02 | LLAVFQSASKII | LLAVFHSASKII | 298 | 727 |
| HLA-A*03:01 | AVFQSASK | AVFHSASK | 795 | 335 |
| HLA-A*03:01 | AVFQSASKIITLK | AVFHSASKIITLK | 74 | 32 |
| HLA-A*03:02 | AVFQSASKIITLKK | AVFHSASKIITLKK | 619 | 272 |
| HLA-A*02:11 | GVALLAVFQSA | GVALLAVFHSA | 895 | 401 |
| HLA-A*68:01 | AVFQSASKIITLK | AVFHSASKIITLK | 808 | 368 |
| HLA-A*03:02 | AVFQSASKIITLK | AVFHSASKIITLK | 170 | 82 |
| HLA-A*02:11 | VALLAVFQSA | VALLAVFHSA | 173 | 85 |
| HLA-A*02:11 | ALLAVFQSAS | ALLAVFHSAS | 691 | 340 |
| HLA-A*02:01 | ALLAVFQSA | ALLAVFHSA | 42 | 21 |
| HLA-B*58:01 | QSASKIITLKKRW | HSASKIITLKKRW | 142 | 71 |
NS8 protein
T11K
Reference MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDP
|||||||||| |||||||||||||||||||||||||
Mutated MKFLVFLGIIKTVAAFHQECSLQSCTQHQPYVVDDP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*03:01 | KFLVFLGIIT | KFLVFLGIIK | 22676 | 110 |
| HLA-A*03:02 | KFLVFLGIIT | KFLVFLGIIK | 23455 | 187 |
| HLA-A*31:01 | KFLVFLGIIT | KFLVFLGIIK | 15005 | 439 |
| HLA-A*68:02 | TTVAAFHQECSL | KTVAAFHQECSL | 288 | 4926 |
| HLA-A*02:06 | FLGIITTV | FLGIIKTV | 134 | 1023 |
| HLA-A*02:01 | FLGIITTV | FLGIIKTV | 42 | 300 |
| HLA-A*02:11 | VFLGIITTV | VFLGIIKTV | 90 | 610 |
| HLA-A*02:01 | KFLVFLGIITTV | KFLVFLGIIKTV | 138 | 863 |
| HLA-A*02:11 | MKFLVFLGIITTV | MKFLVFLGIIKTV | 205 | 1222 |
| HLA-A*02:02 | FLGIITTV | FLGIIKTV | 29 | 172 |
| HLA-A*02:01 | FLVFLGIITTVA | FLVFLGIIKTVA | 184 | 1037 |
| HLA-A*02:01 | FLGIITTVA | FLGIIKTVA | 118 | 654 |
| HLA-A*02:02 | VFLGIITTVA | VFLGIIKTVA | 336 | 1841 |
| HLA-A*02:06 | KFLVFLGIITTV | KFLVFLGIIKTV | 382 | 2032 |
| HLA-A*02:11 | KFLVFLGIITTV | KFLVFLGIIKTV | 36 | 191 |
| HLA-A*02:11 | FLGIITTVA | FLGIIKTVA | 20 | 106 |
| HLA-A*02:11 | FLVFLGIITTVA | FLVFLGIIKTVA | 56 | 288 |
| HLA-A*02:02 | KFLVFLGIITTV | KFLVFLGIIKTV | 215 | 1042 |
| HLA-A*02:06 | FLGIITTVA | FLGIIKTVA | 391 | 1833 |
| HLA-A*02:11 | FLGIITTV | FLGIIKTV | 9 | 42 |
| HLA-A*68:02 | LVFLGIITTV | LVFLGIIKTV | 66 | 308 |
| HLA-A*02:02 | FLGIITTVA | FLGIIKTVA | 27 | 121 |
| HLA-A*02:06 | LVFLGIITTV | LVFLGIIKTV | 31 | 136 |
| HLA-A*02:06 | FLVFLGIITTV | FLVFLGIIKTV | 64 | 271 |
| HLA-A*02:02 | LVFLGIITTV | LVFLGIIKTV | 94 | 380 |
| HLA-A*02:02 | FLVFLGIITTV | FLVFLGIIKTV | 57 | 216 |
| HLA-A*02:11 | LVFLGIITTV | LVFLGIIKTV | 25 | 94 |
| HLA-A*02:01 | FLVFLGIITTV | FLVFLGIIKTV | 25 | 86 |
| HLA-A*02:01 | LVFLGIITTV | LVFLGIIKTV | 96 | 330 |
| HLA-A*02:02 | FLVFLGIITTVA | FLVFLGIIKTVA | 263 | 830 |
| HLA-A*02:06 | FLVFLGIITT | FLVFLGIIKT | 234 | 554 |
| HLA-A*02:06 | LVFLGIITT | LVFLGIIKT | 366 | 863 |
| HLA-A*02:11 | FLVFLGIITTV | FLVFLGIIKTV | 7 | 16 |
| HLA-A*02:01 | FLVFLGIITT | FLVFLGIIKT | 83 | 180 |
P38S
Reference VAAFHQECSLQSCTQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VAAFHQECSLQSCTQHQPYVVDDPCSIHFYSKWYIRVGARKSAPLIELCVD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | PIHFYSKWYI | SIHFYSKWYI | 2551 | 18 |
| HLA-B*57:01 | CPIHFYSKW | CSIHFYSKW | 2170 | 19 |
| HLA-B*58:01 | CPIHFYSKW | CSIHFYSKW | 874 | 10 |
| HLA-A*68:02 | PIHFYSKWYI | SIHFYSKWYI | 5320 | 69 |
| HLA-B*53:01 | CPIHFYSKW | CSIHFYSKW | 13 | 746 |
| HLA-A*02:01 | PIHFYSKWYI | SIHFYSKWYI | 8653 | 181 |
| HLA-A*02:02 | PIHFYSKWYI | SIHFYSKWYI | 4176 | 91 |
| HLA-B*15:03 | PIHFYSKWY | SIHFYSKWY | 11497 | 283 |
| HLA-A*02:06 | PIHFYSKWYI | SIHFYSKWYI | 9158 | 230 |
| HLA-B*57:01 | VDDPCPIHFYSKW | VDDPCSIHFYSKW | 10172 | 276 |
| HLA-B*58:01 | VDDPCPIHFYSKW | VDDPCSIHFYSKW | 8644 | 283 |
| HLA-B*15:01 | PIHFYSKWY | SIHFYSKWY | 9315 | 397 |
| HLA-A*32:01 | CPIHFYSKW | CSIHFYSKW | 7542 | 353 |
| HLA-B*57:01 | PCPIHFYSKW | PCSIHFYSKW | 5974 | 281 |
| HLA-B*58:01 | PCPIHFYSKW | PCSIHFYSKW | 6211 | 342 |
| HLA-A*30:02 | PIHFYSKWY | SIHFYSKWY | 2072 | 126 |
| HLA-A*29:02 | PIHFYSKWY | SIHFYSKWY | 3955 | 247 |
| HLA-A*68:01 | PIHFYSKWYIR | SIHFYSKWYIR | 5448 | 357 |
| HLA-A*31:01 | PIHFYSKWYIRV | SIHFYSKWYIRV | 5629 | 391 |
| HLA-A*30:02 | CPIHFYSKWY | CSIHFYSKWY | 2544 | 177 |
| HLA-B*38:01 | PIHFYSKWYI | SIHFYSKWYI | 5241 | 468 |
| HLA-A*31:01 | PIHFYSKWYIR | SIHFYSKWYIR | 158 | 19 |
| HLA-A*33:03 | PIHFYSKWYIR | SIHFYSKWYIR | 398 | 70 |
| HLA-A*68:02 | YVVDDPCPI | YVVDDPCSI | 57 | 233 |
| HLA-A*02:01 | YVVDDPCPI | YVVDDPCSI | 59 | 230 |
| HLA-A*29:02 | CPIHFYSKWY | CSIHFYSKWY | 1756 | 460 |
| HLA-A*02:06 | YVVDDPCPI | YVVDDPCSI | 10 | 31 |
| HLA-A*02:11 | YVVDDPCPI | YVVDDPCSI | 13 | 37 |
| HLA-A*68:01 | CPIHFYSKWYIR | CSIHFYSKWYIR | 1111 | 419 |
| HLA-A*02:02 | YVVDDPCPI | YVVDDPCSI | 29 | 74 |
| HLA-A*31:01 | CPIHFYSKWYIR | CSIHFYSKWYIR | 74 | 30 |
| HLA-A*31:01 | PCPIHFYSKWYIR | PCSIHFYSKWYIR | 725 | 314 |
| HLA-A*01:01 | VVDDPCPIHFY | VVDDPCSIHFY | 225 | 99 |
S67F
Reference YSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQE
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated YSKWYIRVGARKSAPLIELCVDEAGFKSPIQYIDIGNYTVSCLPFTINCQE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | GSKSPIQYI | GFKSPIQYI | 47 | 578 |
| HLA-B*15:03 | AGSKSPIQY | AGFKSPIQY | 820 | 77 |
| HLA-B*18:01 | DEAGSKSPIQY | DEAGFKSPIQY | 754 | 87 |
| HLA-B*18:01 | DEAGSKSPI | DEAGFKSPI | 1348 | 162 |
| HLA-A*68:01 | ELCVDEAGSK | ELCVDEAGFK | 1733 | 333 |
| HLA-A*30:01 | AGSKSPIQYI | AGFKSPIQYI | 192 | 878 |
| HLA-A*30:02 | AGSKSPIQY | AGFKSPIQY | 726 | 295 |
| HLA-C*16:01 | AGSKSPIQY | AGFKSPIQY | 1080 | 457 |
NS9b protein
R32L
Reference EMHPALRLVDPQIQLAVTRMENAVGRDQNNVGPKVYPIILRLGSPLSLNMA
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated EMHPALRLVDPQIQLAVTRMENAVGLDQNNVGPKVYPIILRLGSPLSLNMA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | VTRMENAVGR | VTRMENAVGL | 28 | 17011 |
| HLA-A*31:01 | VTRMENAVGR | VTRMENAVGL | 113 | 18879 |
| HLA-A*02:11 | QLAVTRMENAVGR | QLAVTRMENAVGL | 19795 | 123 |
| HLA-A*68:01 | LAVTRMENAVGR | LAVTRMENAVGL | 207 | 30953 |
| HLA-A*33:03 | VTRMENAVGR | VTRMENAVGL | 218 | 25621 |
| HLA-C*06:02 | TRMENAVGR | TRMENAVGL | 14329 | 155 |
| HLA-A*11:01 | VTRMENAVGR | VTRMENAVGL | 268 | 21072 |
| HLA-A*02:02 | QLAVTRMENAVGR | QLAVTRMENAVGL | 19025 | 243 |
| HLA-B*38:01 | TRMENAVGR | TRMENAVGL | 29996 | 403 |
| HLA-C*07:01 | TRMENAVGR | TRMENAVGL | 10136 | 175 |
| HLA-C*07:02 | TRMENAVGR | TRMENAVGL | 11506 | 255 |
| HLA-A*02:11 | GRDQNNVGPKV | GLDQNNVGPKV | 13729 | 321 |
| HLA-A*02:11 | GRDQNNVGPKVYPI | GLDQNNVGPKVYPI | 13047 | 313 |
| HLA-A*68:02 | VTRMENAVGR | VTRMENAVGL | 17661 | 439 |
| HLA-C*14:02 | TRMENAVGR | TRMENAVGL | 15120 | 469 |
| HLA-A*68:02 | NAVGRDQNNV | NAVGLDQNNV | 1423 | 248 |
| HLA-A*30:01 | VTRMENAVGR | VTRMENAVGL | 1092 | 473 |
NS9c protein
L52F
Reference VKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||
Mutated VKPLLVPHHVVATVQEIQLQAAVGEFLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | QLQAAVGEL | QLQAAVGEF | 120 | 5031 |
| HLA-A*02:11 | GELLLLEWLA | GEFLLLEWLA | 267 | 9767 |
| HLA-B*15:01 | QLQAAVGEL | QLQAAVGEF | 2048 | 65 |
| HLA-A*02:01 | GELLLLEWLA | GEFLLLEWLA | 409 | 12481 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVGEF | 92 | 2789 |
| HLA-B*15:03 | QLQAAVGEL | QLQAAVGEF | 2231 | 81 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVGEF | 170 | 4413 |
| HLA-A*02:11 | ELLLLEWLA | EFLLLEWLA | 345 | 8719 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVGEF | 338 | 7506 |
| HLA-B*15:01 | IQLQAAVGEL | IQLQAAVGEF | 467 | 24 |
| HLA-B*15:03 | IQLQAAVGEL | IQLQAAVGEF | 168 | 10 |
| HLA-B*15:03 | LQAAVGEL | LQAAVGEF | 295 | 18 |
| HLA-B*15:03 | VQEIQLQAAVGEL | VQEIQLQAAVGEF | 6575 | 430 |
| HLA-A*02:11 | LLLLEWLAM | FLLLEWLAM | 283 | 20 |
| HLA-B*40:01 | QEIQLQAAVGEL | QEIQLQAAVGEF | 201 | 2708 |
| HLA-B*15:01 | LQAAVGEL | LQAAVGEF | 1712 | 138 |
| HLA-A*02:01 | LLLLEWLAM | FLLLEWLAM | 573 | 54 |
| HLA-A*02:11 | LLLLEWLA | FLLLEWLA | 844 | 82 |
| HLA-A*02:02 | LLLLEWLAM | FLLLEWLAM | 1379 | 150 |
| HLA-B*44:03 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1386 | 164 |
| HLA-A*02:06 | LLLLEWLAM | FLLLEWLAM | 763 | 94 |
| HLA-A*02:01 | LLLLEWLA | FLLLEWLA | 1287 | 163 |
| HLA-B*44:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1403 | 179 |
| HLA-A*02:02 | LLLLEWLA | FLLLEWLA | 2866 | 389 |
| HLA-C*03:02 | LLLLEWLAM | FLLLEWLAM | 2566 | 358 |
| HLA-A*02:06 | IQLQAAVGEL | IQLQAAVGEF | 280 | 1893 |
| HLA-A*02:06 | LLLLEWLA | FLLLEWLA | 2803 | 489 |
| HLA-A*02:02 | LLLLEWLAMA | FLLLEWLAMA | 67 | 13 |
| HLA-A*02:01 | ELLLLEWLAMA | EFLLLEWLAMA | 1276 | 266 |
| HLA-A*02:11 | QLQAAVGELLL | QLQAAVGEFLL | 733 | 157 |
| HLA-B*40:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 334 | 1488 |
| HLA-A*02:06 | LLLLEWLAMA | FLLLEWLAMA | 49 | 11 |
| HLA-A*02:11 | LLLLEWLAMAVM | FLLLEWLAMAVM | 926 | 212 |
| HLA-A*02:02 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 714 | 164 |
| HLA-A*02:01 | LLLLEWLAMAVML | FLLLEWLAMAVML | 1353 | 320 |
| HLA-A*02:11 | LLLLEWLAMAVML | FLLLEWLAMAVML | 385 | 97 |
| HLA-A*02:11 | LQAAVGELLL | LQAAVGEFLL | 1331 | 346 |
| HLA-A*02:01 | LLLLEWLAMA | FLLLEWLAMA | 23 | 6 |
| HLA-A*02:11 | ELLLLEWLAMA | EFLLLEWLAMA | 299 | 79 |
| HLA-A*02:11 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 544 | 146 |
| HLA-A*02:01 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 1361 | 373 |
| HLA-B*40:01 | GELLLLEWLAM | GEFLLLEWLAM | 791 | 222 |
| HLA-B*15:03 | AAVGELLLL | AAVGEFLLL | 1475 | 426 |
| HLA-A*02:01 | QLQAAVGELLL | QLQAAVGEFLL | 1401 | 441 |
| HLA-A*02:02 | LLLLEWLAMAV | FLLLEWLAMAV | 453 | 147 |
| HLA-C*03:03 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-C*03:04 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-A*02:02 | QLQAAVGELLL | QLQAAVGEFLL | 530 | 186 |
| HLA-B*50:01 | GELLLLEWLA | GEFLLLEWLA | 1298 | 484 |
| HLA-A*02:11 | LLLLEWLAMA | FLLLEWLAMA | 8 | 3 |
| HLA-A*02:01 | LQAAVGELLL | LQAAVGEFLL | 917 | 347 |
| HLA-C*03:03 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-C*03:04 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-A*02:06 | LQAAVGELLL | LQAAVGEFLL | 162 | 63 |
| HLA-B*40:06 | GELLLLEWLA | GEFLLLEWLA | 268 | 106 |
| HLA-B*40:02 | GELLLLEWLAM | GEFLLLEWLAM | 201 | 83 |
| HLA-A*02:02 | LQAAVGELLL | LQAAVGEFLL | 100 | 42 |
| HLA-C*03:02 | AAVGELLLL | AAVGEFLLL | 269 | 116 |
| HLA-A*02:01 | LLLLEWLAMAV | FLLLEWLAMAV | 97 | 44 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVGEFL | 303 | 139 |
| HLA-B*15:01 | LQAAVGELLL | LQAAVGEFLL | 109 | 237 |
| HLA-B*15:03 | LQAAVGELLL | LQAAVGEFLL | 98 | 210 |
| HLA-A*02:11 | LQAAVGELL | LQAAVGEFL | 386 | 183 |
| HLA-B*40:02 | GELLLLEWLAMAV | GEFLLLEWLAMAV | 815 | 391 |
| HLA-B*40:02 | GELLLLEWLA | GEFLLLEWLA | 475 | 231 |
| HLA-A*02:06 | IQLQAAVGELLL | IQLQAAVGEFLL | 626 | 310 |
| HLA-C*16:01 | AAVGELLLL | AAVGEFLLL | 205 | 102 |
NSP3 protein
T237A
Reference GYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATN
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GYLKLTDNVYIKNADIVEEAKKVKPAVVVNAANVYLKHGGGVAGALNKATN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | TVVVNAANVYLK | AVVVNAANVYLK | 60 | 770 |
| HLA-B*45:01 | EEAKKVKPT | EEAKKVKPA | 1486 | 130 |
| HLA-A*68:02 | TVVVNAANV | AVVVNAANV | 33 | 362 |
| HLA-B*07:02 | KPTVVVNAA | KPAVVVNAA | 298 | 89 |
| HLA-B*42:01 | KPTVVVNAA | KPAVVVNAA | 84 | 33 |
| HLA-B*42:01 | KPTVVVNAANV | KPAVVVNAANV | 1172 | 486 |
| HLA-B*42:01 | KPTVVVNAANVYL | KPAVVVNAANVYL | 321 | 137 |
| HLA-A*02:11 | TVVVNAANV | AVVVNAANV | 617 | 282 |
| HLA-B*07:02 | KPTVVVNAANVYL | KPAVVVNAANVYL | 770 | 354 |
T720I
Reference DWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated DWSYSGQSTQLGIEFLKRGDKSVYYISNPTTFHLDGEVITFDNLKTLLSLR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | RGDKSVYYT | RGDKSVYYI | 9902 | 417 |
| HLA-A*02:11 | EFLKRGDKSVYYT | EFLKRGDKSVYYI | 2936 | 129 |
| HLA-A*02:11 | FLKRGDKSVYYTS | FLKRGDKSVYYIS | 3436 | 227 |
| HLA-A*02:02 | FLKRGDKSVYYT | FLKRGDKSVYYI | 435 | 34 |
| HLA-A*02:01 | FLKRGDKSVYYT | FLKRGDKSVYYI | 420 | 35 |
| HLA-A*02:11 | FLKRGDKSVYYT | FLKRGDKSVYYI | 61 | 6 |
| HLA-A*02:06 | FLKRGDKSVYYT | FLKRGDKSVYYI | 1489 | 152 |
| HLA-A*02:11 | YTSNPTTFHL | YISNPTTFHL | 158 | 19 |
| HLA-C*16:01 | YTSNPTTF | YISNPTTF | 90 | 744 |
| HLA-A*02:01 | YTSNPTTFHL | YISNPTTFHL | 679 | 87 |
| HLA-B*58:01 | TSNPTTFHL | ISNPTTFHL | 184 | 24 |
| HLA-A*23:01 | YYTSNPTTFH | YYISNPTTFH | 721 | 97 |
| HLA-B*57:01 | TSNPTTFHL | ISNPTTFHL | 1577 | 259 |
| HLA-A*68:02 | YTSNPTTFHL | YISNPTTFHL | 31 | 164 |
| HLA-A*68:02 | SVYYTSNPT | SVYYISNPT | 244 | 49 |
| HLA-A*02:02 | YTSNPTTFHL | YISNPTTFHL | 64 | 13 |
| HLA-A*24:02 | YYTSNPTTFH | YYISNPTTFH | 530 | 108 |
| HLA-A*02:11 | YTSNPTTFHLDGEV | YISNPTTFHLDGEV | 2432 | 496 |
| HLA-A*02:02 | YTSNPTTFHLDGEV | YISNPTTFHLDGEV | 2099 | 434 |
| HLA-A*23:01 | YYTSNPTTFHLD | YYISNPTTFHLD | 1800 | 381 |
| HLA-A*02:02 | YYTSNPTTFHL | YYISNPTTFHL | 1864 | 396 |
| HLA-A*23:01 | YYTSNPTTFHL | YYISNPTTFHL | 105 | 23 |
| HLA-A*68:01 | YTSNPTTFH | YISNPTTFH | 392 | 1768 |
| HLA-A*23:01 | KSVYYTSNPTTF | KSVYYISNPTTF | 436 | 99 |
| HLA-A*24:02 | KSVYYTSNPTTF | KSVYYISNPTTF | 453 | 105 |
| HLA-A*68:02 | TSNPTTFHL | ISNPTTFHL | 410 | 1689 |
| HLA-A*68:02 | SVYYTSNPTT | SVYYISNPTT | 1475 | 367 |
| HLA-B*15:03 | YYTSNPTTF | YYISNPTTF | 197 | 54 |
| HLA-A*68:02 | YTSNPTTFHLDGEV | YISNPTTFHLDGEV | 200 | 718 |
| HLA-C*03:03 | TSNPTTFHL | ISNPTTFHL | 172 | 49 |
| HLA-C*03:04 | TSNPTTFHL | ISNPTTFHL | 172 | 49 |
| HLA-C*12:02 | YYTSNPTTF | YYISNPTTF | 1053 | 306 |
| HLA-B*15:03 | TSNPTTFHL | ISNPTTFHL | 968 | 290 |
| HLA-C*03:02 | YYTSNPTTF | YYISNPTTF | 226 | 69 |
| HLA-A*23:01 | YYTSNPTTF | YYISNPTTF | 19 | 6 |
| HLA-C*14:02 | YYTSNPTT | YYISNPTT | 1456 | 460 |
| HLA-A*29:02 | YYTSNPTTF | YYISNPTTF | 372 | 118 |
| HLA-C*14:02 | YYTSNPTTFH | YYISNPTTFH | 91 | 29 |
| HLA-B*35:01 | YYTSNPTTF | YYISNPTTF | 1149 | 373 |
| HLA-C*03:02 | TSNPTTFHL | ISNPTTFHL | 249 | 82 |
| HLA-C*12:03 | YYTSNPTTF | YYISNPTTF | 546 | 185 |
| HLA-C*14:02 | YTSNPTTF | YISNPTTF | 641 | 229 |
| HLA-C*14:02 | KSVYYTSNPTTF | KSVYYISNPTTF | 591 | 212 |
| HLA-A*24:02 | SVYYTSNPTTF | SVYYISNPTTF | 304 | 116 |
| HLA-A*24:02 | YYTSNPTTFHL | YYISNPTTFHL | 110 | 42 |
| HLA-C*12:02 | TSNPTTFHL | ISNPTTFHL | 914 | 356 |
| HLA-C*17:01 | TSNPTTFHL | ISNPTTFHL | 161 | 63 |
| HLA-C*15:02 | TSNPTTFHL | ISNPTTFHL | 91 | 36 |
| HLA-C*03:02 | YTSNPTTFH | YISNPTTFH | 313 | 790 |
| HLA-C*02:02 | TSNPTTFHL | ISNPTTFHL | 810 | 332 |
| HLA-C*02:10 | TSNPTTFHL | ISNPTTFHL | 810 | 332 |
| HLA-C*14:02 | YYTSNPTTFHL | YYISNPTTFHL | 50 | 21 |
| HLA-A*02:06 | SVYYTSNPT | SVYYISNPT | 926 | 389 |
| HLA-A*68:02 | SVYYTSNPTTFHL | SVYYISNPTTFHL | 904 | 386 |
| HLA-A*24:02 | YYTSNPTTF | YYISNPTTF | 14 | 6 |
| HLA-A*23:01 | SVYYTSNPTTF | SVYYISNPTTF | 201 | 88 |
| HLA-C*16:01 | TSNPTTFHL | ISNPTTFHL | 66 | 29 |
| HLA-C*03:02 | YTSNPTTF | YISNPTTF | 110 | 247 |
| HLA-C*14:02 | SVYYTSNPTTF | SVYYISNPTTF | 156 | 71 |
| HLA-C*16:01 | YYTSNPTTF | YYISNPTTF | 378 | 177 |
| HLA-B*15:01 | SVYYTSNPTTF | SVYYISNPTTF | 107 | 227 |
E997K
Reference GKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHIT
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GKQATKYLVQQESPFVMMSAPPAQYKLKHGTFTCASEYTGNYQCGHYKHIT
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | MMSAPPAQYE | MMSAPPAQYK | 11142 | 11 |
| HLA-A*11:01 | MSAPPAQYE | MSAPPAQYK | 7985 | 8 |
| HLA-A*11:01 | VMMSAPPAQYE | VMMSAPPAQYK | 16642 | 21 |
| HLA-A*03:01 | MMSAPPAQYE | MMSAPPAQYK | 4305 | 7 |
| HLA-A*03:02 | VMMSAPPAQYE | VMMSAPPAQYK | 10146 | 18 |
| HLA-A*11:01 | FVMMSAPPAQYE | FVMMSAPPAQYK | 20158 | 37 |
| HLA-A*03:02 | MMSAPPAQYE | MMSAPPAQYK | 3256 | 6 |
| HLA-A*03:01 | MSAPPAQYE | MSAPPAQYK | 15404 | 32 |
| HLA-A*03:01 | VMMSAPPAQYE | VMMSAPPAQYK | 8547 | 20 |
| HLA-A*03:02 | MSAPPAQYE | MSAPPAQYK | 9402 | 29 |
| HLA-A*03:02 | FVMMSAPPAQYE | FVMMSAPPAQYK | 18663 | 67 |
| HLA-A*68:01 | FVMMSAPPAQYE | FVMMSAPPAQYK | 6251 | 30 |
| HLA-A*03:01 | FVMMSAPPAQYE | FVMMSAPPAQYK | 18583 | 112 |
| HLA-A*68:01 | MMSAPPAQYE | MMSAPPAQYK | 2018 | 13 |
| HLA-A*11:01 | SPFVMMSAPPAQYE | SPFVMMSAPPAQYK | 31065 | 215 |
| HLA-A*68:01 | MSAPPAQYE | MSAPPAQYK | 607 | 6 |
| HLA-A*30:01 | MSAPPAQYE | MSAPPAQYK | 3626 | 41 |
| HLA-B*18:01 | YELKHGTF | YKLKHGTF | 197 | 16931 |
| HLA-A*11:01 | MSAPPAQYEL | MSAPPAQYKL | 16224 | 201 |
| HLA-A*30:01 | ELKHGTFTCA | KLKHGTFTCA | 12076 | 155 |
| HLA-B*40:02 | YELKHGTFTC | YKLKHGTFTC | 285 | 21340 |
| HLA-A*31:01 | MSAPPAQYE | MSAPPAQYK | 11290 | 155 |
| HLA-B*40:02 | YELKHGTFT | YKLKHGTFT | 333 | 23741 |
| HLA-A*30:01 | MMSAPPAQYE | MMSAPPAQYK | 7805 | 113 |
| HLA-A*31:01 | MMSAPPAQYE | MMSAPPAQYK | 9351 | 143 |
| HLA-A*74:01 | MMSAPPAQYE | MMSAPPAQYK | 16063 | 297 |
| HLA-A*68:01 | PFVMMSAPPAQYE | PFVMMSAPPAQYK | 23690 | 446 |
| HLA-A*03:02 | MMSAPPAQYEL | MMSAPPAQYKL | 13746 | 268 |
| HLA-A*68:01 | VMMSAPPAQYE | VMMSAPPAQYK | 20285 | 415 |
| HLA-A*30:01 | VMMSAPPAQYE | VMMSAPPAQYK | 15000 | 307 |
| HLA-A*03:02 | VMMSAPPAQYEL | VMMSAPPAQYKL | 14616 | 312 |
| HLA-A*03:01 | MMSAPPAQYEL | MMSAPPAQYKL | 16300 | 377 |
| HLA-A*31:01 | VMMSAPPAQYE | VMMSAPPAQYK | 14950 | 366 |
| HLA-A*30:01 | ELKHGTFTC | KLKHGTFTC | 18686 | 478 |
| HLA-B*50:01 | YELKHGTFTCA | YKLKHGTFTCA | 459 | 15951 |
| HLA-A*03:01 | VMMSAPPAQYEL | VMMSAPPAQYKL | 14996 | 432 |
| HLA-B*50:01 | YELKHGTFT | YKLKHGTFT | 389 | 12837 |
| HLA-A*33:03 | MSAPPAQYE | MSAPPAQYK | 9728 | 371 |
| HLA-A*02:11 | ELKHGTFTC | KLKHGTFTC | 5683 | 238 |
| HLA-A*68:01 | MSAPPAQYEL | MSAPPAQYKL | 4777 | 201 |
| HLA-A*33:03 | MMSAPPAQYE | MMSAPPAQYK | 9695 | 419 |
| HLA-A*02:11 | ELKHGTFTCA | KLKHGTFTCA | 5810 | 352 |
| HLA-A*30:02 | ELKHGTFTCASEY | KLKHGTFTCASEY | 1031 | 65 |
| HLA-B*15:01 | ELKHGTFTCASEY | KLKHGTFTCASEY | 4014 | 316 |
| HLA-A*02:02 | ELKHGTFTCA | KLKHGTFTCA | 3758 | 456 |
| HLA-B*15:03 | YELKHGTF | YKLKHGTF | 588 | 100 |
| HLA-C*03:03 | SAPPAQYEL | SAPPAQYKL | 99 | 524 |
| HLA-C*03:04 | SAPPAQYEL | SAPPAQYKL | 99 | 524 |
| HLA-B*15:03 | YELKHGTFTCASEY | YKLKHGTFTCASEY | 793 | 190 |
| HLA-C*03:02 | SAPPAQYEL | SAPPAQYKL | 430 | 1595 |
| HLA-C*16:01 | SAPPAQYEL | SAPPAQYKL | 69 | 249 |
| HLA-A*02:01 | MMSAPPAQYEL | MMSAPPAQYKL | 172 | 556 |
| HLA-C*01:02 | MSAPPAQYEL | MSAPPAQYKL | 278 | 821 |
| HLA-A*02:11 | MMSAPPAQYEL | MMSAPPAQYKL | 29 | 85 |
| HLA-A*02:02 | FVMMSAPPAQYEL | FVMMSAPPAQYKL | 100 | 267 |
| HLA-C*12:03 | SAPPAQYEL | SAPPAQYKL | 193 | 506 |
| HLA-C*14:02 | SAPPAQYEL | SAPPAQYKL | 433 | 1081 |
| HLA-A*02:02 | VMMSAPPAQYEL | VMMSAPPAQYKL | 37 | 92 |
| HLA-A*02:11 | FVMMSAPPAQYEL | FVMMSAPPAQYKL | 58 | 144 |
| HLA-C*01:02 | SAPPAQYEL | SAPPAQYKL | 21 | 49 |
| HLA-A*68:01 | FVMMSAPPAQYELK | FVMMSAPPAQYKLK | 276 | 644 |
| HLA-A*02:02 | MMSAPPAQYEL | MMSAPPAQYKL | 18 | 41 |
| HLA-A*02:01 | FVMMSAPPAQYEL | FVMMSAPPAQYKL | 283 | 636 |
| HLA-A*02:11 | VMMSAPPAQYEL | VMMSAPPAQYKL | 21 | 47 |
| HLA-A*02:02 | MMSAPPAQYELK | MMSAPPAQYKLK | 338 | 747 |
| HLA-A*02:01 | VMMSAPPAQYEL | VMMSAPPAQYKL | 82 | 180 |
| HLA-A*02:06 | FVMMSAPPAQYEL | FVMMSAPPAQYKL | 86 | 187 |
| HLA-C*03:03 | MSAPPAQYEL | MSAPPAQYKL | 92 | 188 |
| HLA-C*03:04 | MSAPPAQYEL | MSAPPAQYKL | 92 | 188 |
| HLA-C*17:01 | SAPPAQYEL | SAPPAQYKL | 424 | 852 |
NSP4 protein
T492I
Reference ACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQ
||||||||||||||||||||||||| ||||||||
Mutated ACCHLAKALNDFSNSGSDVLYQPPQISITSAVLQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*16:01 | QTSITSAVL | QISITSAVL | 201 | 1829 |
| HLA-A*68:02 | QTSITSAVL | QISITSAVL | 206 | 1702 |
| HLA-A*02:06 | QPPQTSITSAV | QPPQISITSAV | 3097 | 393 |
| HLA-A*02:06 | LYQPPQTSITSAV | LYQPPQISITSAV | 1485 | 214 |
| HLA-A*02:06 | YQPPQTSITSAV | YQPPQISITSAV | 156 | 26 |
| HLA-A*02:01 | YQPPQTSITSAV | YQPPQISITSAV | 1613 | 341 |
| HLA-A*02:11 | YQPPQTSITSAV | YQPPQISITSAV | 498 | 120 |
| HLA-C*03:03 | TSITSAVL | ISITSAVL | 532 | 198 |
| HLA-C*03:04 | TSITSAVL | ISITSAVL | 532 | 198 |
| HLA-C*03:03 | QTSITSAVL | QISITSAVL | 311 | 831 |
| HLA-C*03:04 | QTSITSAVL | QISITSAVL | 311 | 831 |
| HLA-C*16:01 | TSITSAVL | ISITSAVL | 381 | 156 |
| HLA-A*02:01 | VLYQPPQTSI | VLYQPPQISI | 1084 | 474 |
| HLA-A*23:01 | VLYQPPQTSI | VLYQPPQISI | 979 | 462 |
| HLA-A*23:01 | LYQPPQTSI | LYQPPQISI | 278 | 132 |
NSP6 protein
Q160R
Reference GARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GARRVWTLMNVLTLVYKVYYGNALDRAISMWALIISVTSNYSGVVTTVMFL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*31:01 | KVYYGNALDQ | KVYYGNALDR | 12952 | 19 |
| HLA-A*68:01 | KVYYGNALDQ | KVYYGNALDR | 12680 | 70 |
| HLA-A*31:01 | VYYGNALDQ | VYYGNALDR | 24021 | 253 |
| HLA-A*31:01 | YKVYYGNALDQ | YKVYYGNALDR | 30658 | 331 |
| HLA-A*74:01 | KVYYGNALDQ | KVYYGNALDR | 14615 | 163 |
| HLA-A*33:03 | KVYYGNALDQ | KVYYGNALDR | 28338 | 322 |
| HLA-A*03:02 | KVYYGNALDQ | KVYYGNALDR | 5360 | 75 |
| HLA-A*31:01 | VYKVYYGNALDQ | VYKVYYGNALDR | 25960 | 377 |
| HLA-A*11:01 | KVYYGNALDQ | KVYYGNALDR | 8219 | 130 |
| HLA-A*31:01 | LVYKVYYGNALDQ | LVYKVYYGNALDR | 25391 | 422 |
| HLA-A*02:06 | LDQAISMWAL | LDRAISMWAL | 163 | 7690 |
| HLA-A*03:01 | KVYYGNALDQ | KVYYGNALDR | 3533 | 76 |
| HLA-B*27:05 | LDQAISMWAL | LDRAISMWAL | 10137 | 255 |
| HLA-B*15:01 | LDQAISMWAL | LDRAISMWAL | 431 | 11074 |
| HLA-A*02:02 | LDQAISMWAL | LDRAISMWAL | 355 | 8142 |
| HLA-A*30:01 | QAISMWALI | RAISMWALI | 2792 | 137 |
| HLA-B*08:01 | NALDQAISM | NALDRAISM | 7234 | 389 |
| HLA-A*32:01 | QAISMWALI | RAISMWALI | 1158 | 66 |
| HLA-A*32:01 | QAISMWALII | RAISMWALII | 4377 | 270 |
| HLA-A*68:02 | QAISMWALI | RAISMWALI | 48 | 661 |
| HLA-C*15:02 | QAISMWALI | RAISMWALI | 1322 | 143 |
| HLA-C*15:02 | QAISMWALII | RAISMWALII | 2907 | 334 |
| HLA-B*57:01 | QAISMWALI | RAISMWALI | 1392 | 181 |
| HLA-B*57:01 | QAISMWALII | RAISMWALII | 2295 | 301 |
| HLA-B*58:01 | QAISMWALII | RAISMWALII | 582 | 79 |
| HLA-B*58:01 | QAISMWALI | RAISMWALI | 316 | 47 |
| HLA-A*68:02 | QAISMWALII | RAISMWALII | 489 | 3077 |
| HLA-B*15:03 | QAISMWALI | RAISMWALI | 1722 | 291 |
| HLA-B*14:02 | DQAISMWAL | DRAISMWAL | 770 | 144 |
| HLA-C*16:01 | QAISMWAL | RAISMWAL | 755 | 142 |
| HLA-A*02:11 | QAISMWALIISVT | RAISMWALIISVT | 1866 | 440 |
| HLA-C*16:01 | YGNALDQAISM | YGNALDRAISM | 507 | 120 |
| HLA-A*23:01 | QAISMWALI | RAISMWALI | 1647 | 390 |
| HLA-C*16:01 | YYGNALDQAISM | YYGNALDRAISM | 721 | 174 |
| HLA-C*16:01 | QAISMWALI | RAISMWALI | 895 | 227 |
| HLA-C*16:01 | NALDQAISM | NALDRAISM | 45 | 12 |
| HLA-C*17:01 | QAISMWALI | RAISMWALI | 1535 | 419 |
| HLA-A*02:06 | QAISMWALIISV | RAISMWALIISV | 442 | 126 |
| HLA-A*02:11 | QAISMWALI | RAISMWALI | 1483 | 423 |
| HLA-A*02:06 | QAISMWALI | RAISMWALI | 385 | 111 |
| HLA-A*02:01 | QAISMWALIISV | RAISMWALIISV | 163 | 50 |
| HLA-C*12:03 | NALDQAISM | NALDRAISM | 101 | 33 |
| HLA-C*14:02 | NALDQAISM | NALDRAISM | 859 | 284 |
| HLA-A*02:11 | QAISMWALIISV | RAISMWALIISV | 38 | 13 |
| HLA-C*12:03 | YGNALDQAISM | YGNALDRAISM | 1344 | 490 |
| HLA-B*35:01 | NALDQAISM | NALDRAISM | 35 | 89 |
| HLA-C*12:02 | NALDQAISM | NALDRAISM | 511 | 212 |
| HLA-B*35:03 | NALDQAISM | NALDRAISM | 336 | 803 |
| HLA-C*12:03 | QAISMWALI | RAISMWALI | 585 | 247 |
| HLA-A*24:02 | YYGNALDQAI | YYGNALDRAI | 129 | 279 |
| HLA-B*57:01 | NALDQAISMW | NALDRAISMW | 207 | 96 |
| HLA-C*14:02 | YYGNALDQAISM | YYGNALDRAISM | 67 | 32 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |
NSP13 protein
P419S
Reference KHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRC
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Mutated KHYVYIGDPAQLPAPRTLLTKGTLESEYFNSVCRLMKTIGPDMFLGTCRRC
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:01 | PEYFNSVCRL | SEYFNSVCRL | 3543 | 44 |
| HLA-B*40:02 | PEYFNSVCRL | SEYFNSVCRL | 2542 | 37 |
| HLA-C*14:02 | PEYFNSVCRL | SEYFNSVCRL | 4465 | 76 |
| HLA-B*44:03 | PEYFNSVCRL | SEYFNSVCRL | 10714 | 199 |
| HLA-B*44:02 | PEYFNSVCRL | SEYFNSVCRL | 11676 | 232 |
| HLA-B*44:02 | PEYFNSVCRLM | SEYFNSVCRLM | 13895 | 299 |
| HLA-A*68:01 | EPEYFNSVCR | ESEYFNSVCR | 521 | 12 |
| HLA-B*40:01 | PEYFNSVCRLM | SEYFNSVCRLM | 11464 | 297 |
| HLA-B*44:03 | PEYFNSVCRLM | SEYFNSVCRLM | 13813 | 359 |
| HLA-B*40:02 | PEYFNSVCRLM | SEYFNSVCRLM | 8403 | 229 |
| HLA-B*45:01 | LEPEYFNSV | LESEYFNSV | 2619 | 205 |
| HLA-B*50:01 | LEPEYFNSV | LESEYFNSV | 1963 | 170 |
| HLA-B*40:01 | LEPEYFNSV | LESEYFNSV | 1052 | 104 |
| HLA-B*18:01 | LEPEYFNSV | LESEYFNSV | 2322 | 235 |
| HLA-A*33:03 | EPEYFNSVCR | ESEYFNSVCR | 1806 | 198 |
| HLA-B*40:06 | LEPEYFNSV | LESEYFNSV | 1912 | 255 |
| HLA-B*40:02 | LEPEYFNSV | LESEYFNSV | 328 | 49 |
| HLA-A*23:01 | PEYFNSVCRL | SEYFNSVCRL | 989 | 387 |
| HLA-A*02:06 | TLEPEYFNSV | TLESEYFNSV | 33 | 71 |
L581F
Reference CNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ
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Mutated CNVNRFNVAITRAKVGILCIMSDRDFYDKLQFTSLEIPRRNVATLQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | RDLYDKLQFT | RDFYDKLQFT | 46 | 11160 |
| HLA-A*02:11 | SDRDLYDKLQFT | SDRDFYDKLQFT | 144 | 18628 |
| HLA-A*02:02 | RDLYDKLQFT | RDFYDKLQFT | 118 | 12779 |
| HLA-A*02:11 | MSDRDLYDKLQFT | MSDRDFYDKLQFT | 142 | 12614 |
| HLA-A*02:01 | RDLYDKLQFT | RDFYDKLQFT | 287 | 21033 |
| HLA-A*02:11 | DLYDKLQFT | DFYDKLQFT | 234 | 12554 |
| HLA-A*02:02 | SDRDLYDKLQFT | SDRDFYDKLQFT | 394 | 19577 |
| HLA-A*02:02 | MSDRDLYDKLQFT | MSDRDFYDKLQFT | 262 | 11073 |
| HLA-A*02:02 | ILCIMSDRDL | ILCIMSDRDF | 487 | 8105 |
| HLA-A*02:11 | LYDKLQFTSLEI | FYDKLQFTSLEI | 701 | 85 |
| HLA-B*08:01 | DLYDKLQFTSL | DFYDKLQFTSL | 316 | 1860 |
| HLA-C*07:02 | LYDKLQFTSL | FYDKLQFTSL | 2129 | 377 |
| HLA-A*02:01 | LYDKLQFTSLEI | FYDKLQFTSLEI | 1679 | 308 |
| HLA-A*02:11 | IMSDRDLYDKL | IMSDRDFYDKL | 240 | 45 |
| HLA-A*02:02 | LYDKLQFTSLEI | FYDKLQFTSLEI | 1328 | 256 |
| HLA-A*02:01 | IMSDRDLYDKL | IMSDRDFYDKL | 498 | 101 |
| HLA-A*31:01 | IMSDRDLYDK | IMSDRDFYDK | 1369 | 279 |
| HLA-C*14:02 | LYDKLQFTSL | FYDKLQFTSL | 222 | 50 |
| HLA-A*02:02 | IMSDRDLYDKL | IMSDRDFYDKL | 84 | 27 |
| HLA-A*68:01 | IMSDRDLYDK | IMSDRDFYDK | 1412 | 456 |
| HLA-A*33:03 | LYDKLQFTSLEIPR | FYDKLQFTSLEIPR | 1400 | 492 |
| HLA-B*15:03 | IMSDRDLYDKLQF | IMSDRDFYDKLQF | 970 | 355 |
| HLA-B*08:01 | LYDKLQFTSL | FYDKLQFTSL | 837 | 357 |
| HLA-B*57:01 | MSDRDLYDKLQF | MSDRDFYDKLQF | 309 | 145 |
NSP14 protein
V125F
Reference AVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLI
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Mutated AVGTNLPLQLGFSTGVNLVAVPTGYFDTPNNTDFSRVSAKPPPGDQFKHLI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | NLVAVPTGYV | NLVAVPTGYF | 21 | 4908 |
| HLA-A*68:02 | LVAVPTGYV | LVAVPTGYF | 7 | 1599 |
| HLA-A*68:02 | NLVAVPTGYV | NLVAVPTGYF | 17 | 3266 |
| HLA-A*02:11 | LVAVPTGYV | LVAVPTGYF | 56 | 7394 |
| HLA-B*15:01 | LVAVPTGYV | LVAVPTGYF | 6154 | 49 |
| HLA-A*68:02 | TGVNLVAVPTGYV | TGVNLVAVPTGYF | 130 | 12806 |
| HLA-A*02:01 | NLVAVPTGYV | NLVAVPTGYF | 79 | 7221 |
| HLA-B*15:03 | LVAVPTGYV | LVAVPTGYF | 5599 | 66 |
| HLA-A*02:02 | NLVAVPTGYV | NLVAVPTGYF | 26 | 1579 |
| HLA-A*68:02 | STGVNLVAVPTGYV | STGVNLVAVPTGYF | 302 | 15058 |
| HLA-A*02:02 | LVAVPTGYV | LVAVPTGYF | 78 | 3748 |
| HLA-A*02:06 | LVAVPTGYV | LVAVPTGYF | 90 | 3829 |
| HLA-C*03:02 | LVAVPTGYV | LVAVPTGYF | 1768 | 47 |
| HLA-C*14:02 | YVDTPNNTDF | YFDTPNNTDF | 3208 | 96 |
| HLA-B*15:01 | NLVAVPTGYV | NLVAVPTGYF | 7587 | 277 |
| HLA-C*04:01 | YVDTPNNTDF | YFDTPNNTDF | 4100 | 154 |
| HLA-A*02:06 | NLVAVPTGYV | NLVAVPTGYF | 285 | 6331 |
| HLA-C*03:02 | VAVPTGYV | VAVPTGYF | 3578 | 177 |
| HLA-C*05:01 | YVDTPNNTDF | YFDTPNNTDF | 48 | 729 |
| HLA-A*68:01 | YVDTPNNTDFSR | YFDTPNNTDFSR | 104 | 1380 |
| HLA-C*08:02 | YVDTPNNTDF | YFDTPNNTDF | 106 | 1309 |
| HLA-A*26:01 | LVAVPTGYV | LVAVPTGYF | 3838 | 335 |
| HLA-C*15:02 | LVAVPTGYV | LVAVPTGYF | 404 | 4180 |
| HLA-A*31:01 | GYVDTPNNTDFSR | GYFDTPNNTDFSR | 937 | 171 |
| HLA-C*03:02 | YVDTPNNTDF | YFDTPNNTDF | 453 | 2249 |
| HLA-C*02:02 | LVAVPTGYV | LVAVPTGYF | 1063 | 223 |
| HLA-C*02:10 | LVAVPTGYV | LVAVPTGYF | 1063 | 223 |
| HLA-A*23:01 | GYVDTPNNTDF | GYFDTPNNTDF | 2026 | 426 |
| HLA-C*03:03 | LVAVPTGYV | LVAVPTGYF | 2006 | 438 |
| HLA-C*03:04 | LVAVPTGYV | LVAVPTGYF | 2006 | 438 |
| HLA-C*16:01 | VAVPTGYV | VAVPTGYF | 1614 | 358 |
| HLA-A*30:02 | LVAVPTGYV | LVAVPTGYF | 2003 | 446 |
| HLA-C*12:02 | LVAVPTGYV | LVAVPTGYF | 1221 | 302 |
| HLA-C*16:01 | LVAVPTGYV | LVAVPTGYF | 1447 | 366 |
| HLA-A*33:03 | YVDTPNNTDFSR | YFDTPNNTDFSR | 375 | 163 |