Summary
This strain contains 23 protein-level mutations:
- 12 mutations in Spike protein: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F
- 2 mutations in N protein: P80R, 203-204 RG->KR
- 1 mutation in NS3 protein: S253P
- 1 mutation in NS8 protein: E92K
- 1 mutation in NS9b protein: Q77E
- 1 mutation in NS9c protein: 49-50 VG->LN
- 2 mutations in NSP3 protein: S370L, K977Q
- 1 mutation in NSP6 protein: SGF 106-108 deletion
- 1 mutation in NSP12 protein: P323L
- 1 mutation in NSP13 protein: E341D
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
L18F
Reference MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVF
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Mutated MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | LVSSQCVNL | LVSSQCVNF | 240 | 8391 |
| HLA-C*14:02 | NLTTRTQL | NFTNRTQL | 11573 | 415 |
| HLA-A*02:11 | VLLPLVSSQCVNL | VLLPLVSSQCVNF | 145 | 3640 |
| HLA-B*15:01 | LVSSQCVNL | LVSSQCVNF | 5243 | 220 |
| HLA-A*02:02 | LLPLVSSQCVNL | LLPLVSSQCVNF | 254 | 5928 |
| HLA-A*02:02 | VLLPLVSSQCVNL | VLLPLVSSQCVNF | 417 | 8137 |
| HLA-C*03:02 | LTTRTQLPPAY | FTNRTQLPSAY | 3676 | 208 |
| HLA-B*15:03 | LVSSQCVNL | LVSSQCVNF | 4324 | 259 |
| HLA-A*30:01 | VNLTTRTQLPPA | VNFTNRTQLPSA | 254 | 4040 |
| HLA-A*02:01 | VLLPLVSSQCVNL | VLLPLVSSQCVNF | 418 | 6527 |
| HLA-A*01:01 | LTTRTQLPPAY | FTNRTQLPSAY | 827 | 115 |
| HLA-B*15:01 | LTTRTQLPPAY | FTNRTQLPSAY | 2378 | 368 |
| HLA-A*29:02 | LTTRTQLPPAY | FTNRTQLPSAY | 1225 | 223 |
| HLA-B*42:01 | LPLVSSQCVNL | LPLVSSQCVNF | 415 | 1797 |
| HLA-A*30:01 | LTTRTQLPPA | FTNRTQLPSA | 82 | 318 |
| HLA-B*15:03 | LTTRTQLPPAY | FTNRTQLPSAY | 1683 | 495 |
| HLA-B*58:01 | VSSQCVNLTT | VSSQCVNFTN | 1219 | 416 |
| HLA-A*33:03 | SSQCVNLTTR | SSQCVNFTNR | 283 | 123 |
| HLA-A*31:01 | SQCVNLTTR | SQCVNFTNR | 105 | 47 |
| HLA-A*30:02 | LTTRTQLPPAY | FTNRTQLPSAY | 686 | 324 |
T20N
Reference MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRS
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Mutated MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVFRS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | NLTTRTQL | NFTNRTQL | 11573 | 415 |
| HLA-A*30:01 | TTRTQLPPA | TNRTQLPSA | 9 | 240 |
| HLA-A*30:01 | TTRTQLPPAY | TNRTQLPSAY | 263 | 4995 |
| HLA-C*03:02 | LTTRTQLPPAY | FTNRTQLPSAY | 3676 | 208 |
| HLA-A*30:01 | VNLTTRTQLPPA | VNFTNRTQLPSA | 254 | 4040 |
| HLA-A*01:01 | LTTRTQLPPAY | FTNRTQLPSAY | 827 | 115 |
| HLA-B*15:01 | LTTRTQLPPAY | FTNRTQLPSAY | 2378 | 368 |
| HLA-A*29:02 | LTTRTQLPPAY | FTNRTQLPSAY | 1225 | 223 |
| HLA-A*30:01 | LTTRTQLPPA | FTNRTQLPSA | 82 | 318 |
| HLA-B*15:03 | LTTRTQLPPAY | FTNRTQLPSAY | 1683 | 495 |
| HLA-A*30:02 | TTRTQLPPAY | TNRTQLPSAY | 288 | 883 |
| HLA-B*58:01 | VSSQCVNLTT | VSSQCVNFTN | 1219 | 416 |
| HLA-B*15:03 | TRTQLPPAY | NRTQLPSAY | 158 | 364 |
| HLA-A*33:03 | SSQCVNLTTR | SSQCVNFTNR | 283 | 123 |
| HLA-A*31:01 | SQCVNLTTR | SQCVNFTNR | 105 | 47 |
| HLA-A*30:02 | LTTRTQLPPAY | FTNRTQLPSAY | 686 | 324 |
P26S
Reference MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHST
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Mutated MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVFRSSVLHST
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | PAYTNSFTR | SAYTNSFTR | 2670 | 33 |
| HLA-B*15:03 | PAYTNSFTRGVY | SAYTNSFTRGVY | 4473 | 78 |
| HLA-C*16:01 | PAYTNSFTRGVY | SAYTNSFTRGVY | 6195 | 147 |
| HLA-C*03:02 | PAYTNSFTRGVY | SAYTNSFTRGVY | 5003 | 124 |
| HLA-B*35:01 | PAYTNSFTRGVY | SAYTNSFTRGVY | 13891 | 421 |
| HLA-C*16:01 | PAYTNSFTRGVYY | SAYTNSFTRGVYY | 7665 | 237 |
| HLA-A*31:01 | PAYTNSFTR | SAYTNSFTR | 764 | 25 |
| HLA-C*03:02 | PAYTNSFTRGVYY | SAYTNSFTRGVYY | 7472 | 253 |
| HLA-A*30:01 | TTRTQLPPA | TNRTQLPSA | 9 | 240 |
| HLA-A*68:01 | PAYTNSFTRG | SAYTNSFTRG | 10689 | 474 |
| HLA-B*15:03 | PAYTNSFTRGVYY | SAYTNSFTRGVYY | 5996 | 271 |
| HLA-A*03:02 | PAYTNSFTR | SAYTNSFTR | 4531 | 206 |
| HLA-A*68:01 | PAYTNSFTR | SAYTNSFTR | 165 | 8 |
| HLA-B*53:01 | LPPAYTNSF | LPSAYTNSF | 366 | 18 |
| HLA-A*30:01 | TTRTQLPPAY | TNRTQLPSAY | 263 | 4995 |
| HLA-C*12:03 | PAYTNSFTRGVY | SAYTNSFTRGVY | 7698 | 431 |
| HLA-C*03:02 | LTTRTQLPPAY | FTNRTQLPSAY | 3676 | 208 |
| HLA-B*15:01 | PAYTNSFTRGVY | SAYTNSFTRGVY | 4911 | 286 |
| HLA-A*30:01 | VNLTTRTQLPPA | VNFTNRTQLPSA | 254 | 4040 |
| HLA-A*68:02 | PAYTNSFTRGV | SAYTNSFTRGV | 6906 | 453 |
| HLA-A*33:03 | PAYTNSFTR | SAYTNSFTR | 509 | 39 |
| HLA-B*35:01 | QLPPAYTNSF | QLPSAYTNSF | 3994 | 360 |
| HLA-B*35:01 | LPPAYTNSF | LPSAYTNSF | 43 | 4 |
| HLA-A*30:02 | PAYTNSFTRGVYY | SAYTNSFTRGVYY | 1100 | 108 |
| HLA-A*29:02 | PAYTNSFTRGVY | SAYTNSFTRGVY | 3169 | 338 |
| HLA-A*29:02 | PAYTNSFTRGVYY | SAYTNSFTRGVYY | 925 | 100 |
| HLA-B*07:02 | RTQLPPAYTNSF | RTQLPSAYTNSF | 1180 | 150 |
| HLA-A*30:02 | PAYTNSFTRGVY | SAYTNSFTRGVY | 616 | 82 |
| HLA-A*01:01 | LTTRTQLPPAY | FTNRTQLPSAY | 827 | 115 |
| HLA-A*68:01 | PPAYTNSFTR | PSAYTNSFTR | 864 | 129 |
| HLA-B*35:01 | LPPAYTNSFTRGVY | LPSAYTNSFTRGVY | 1763 | 264 |
| HLA-B*15:01 | LTTRTQLPPAY | FTNRTQLPSAY | 2378 | 368 |
| HLA-B*15:03 | LPPAYTNSF | LPSAYTNSF | 788 | 125 |
| HLA-B*07:02 | LPPAYTNSF | LPSAYTNSF | 293 | 50 |
| HLA-A*29:02 | LTTRTQLPPAY | FTNRTQLPSAY | 1225 | 223 |
| HLA-B*35:03 | LPPAYTNSF | LPSAYTNSF | 1350 | 252 |
| HLA-B*42:01 | QLPPAYTNSF | QLPSAYTNSF | 1191 | 244 |
| HLA-B*42:01 | LPPAYTNSF | LPSAYTNSF | 65 | 14 |
| HLA-B*15:03 | QLPPAYTNSF | QLPSAYTNSF | 1522 | 341 |
| HLA-B*42:01 | RTQLPPAYTNSF | RTQLPSAYTNSF | 825 | 185 |
| HLA-A*30:01 | LTTRTQLPPA | FTNRTQLPSA | 82 | 318 |
| HLA-B*15:03 | LTTRTQLPPAY | FTNRTQLPSAY | 1683 | 495 |
| HLA-A*30:02 | TTRTQLPPAY | TNRTQLPSAY | 288 | 883 |
| HLA-B*15:01 | QLPPAYTNSF | QLPSAYTNSF | 300 | 103 |
| HLA-C*03:02 | LPPAYTNSF | LPSAYTNSF | 1157 | 412 |
| HLA-B*15:03 | TRTQLPPAY | NRTQLPSAY | 158 | 364 |
| HLA-A*30:02 | LTTRTQLPPAY | FTNRTQLPSAY | 686 | 324 |
| HLA-B*58:01 | RTQLPPAYTNSF | RTQLPSAYTNSF | 1040 | 492 |
D138Y
Reference KTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSA
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Mutated KTQSLLIVNNATNVVIKVCEFQFCNYPFLGVYYHKNNKSWMESEFRVYSSA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | NDPFLGVYY | NYPFLGVYY | 18829 | 39 |
| HLA-A*30:02 | KVCEFQFCND | KVCEFQFCNY | 17923 | 53 |
| HLA-A*29:02 | KVCEFQFCND | KVCEFQFCNY | 32767 | 165 |
| HLA-B*18:01 | CEFQFCND | CEFQFCNY | 11492 | 65 |
| HLA-A*11:01 | KVCEFQFCND | KVCEFQFCNY | 30138 | 228 |
| HLA-C*14:02 | NDPFLGVY | NYPFLGVY | 20112 | 191 |
| HLA-C*07:02 | NDPFLGVYY | NYPFLGVYY | 23019 | 404 |
| HLA-A*03:02 | KVCEFQFCND | KVCEFQFCNY | 25740 | 454 |
| HLA-B*35:01 | DPFLGVYYH | YPFLGVYYH | 992 | 21 |
| HLA-B*35:01 | DPFLGVYY | YPFLGVYY | 1593 | 35 |
| HLA-B*15:03 | CNDPFLGVY | CNYPFLGVY | 1048 | 37 |
| HLA-A*01:01 | CNDPFLGVY | CNYPFLGVY | 232 | 4976 |
| HLA-A*29:02 | NDPFLGVYY | NYPFLGVYY | 2130 | 101 |
| HLA-B*15:01 | CNDPFLGVY | CNYPFLGVY | 9489 | 500 |
| HLA-A*01:01 | CNDPFLGVYY | CNYPFLGVYY | 202 | 3648 |
| HLA-B*15:03 | CNDPFLGVYY | CNYPFLGVYY | 4293 | 346 |
| HLA-A*23:01 | EFQFCNDPF | EFQFCNYPF | 1283 | 109 |
| HLA-A*31:01 | DPFLGVYYHK | YPFLGVYYHK | 2222 | 224 |
| HLA-A*01:01 | FCNDPFLGVY | FCNYPFLGVY | 317 | 3011 |
| HLA-A*68:01 | DPFLGVYYHK | YPFLGVYYHK | 398 | 46 |
| HLA-A*29:02 | EFQFCNDPF | EFQFCNYPF | 2275 | 288 |
| HLA-A*24:02 | EFQFCNDPF | EFQFCNYPF | 1400 | 194 |
| HLA-B*15:03 | FCNDPFLGVY | FCNYPFLGVY | 567 | 79 |
| HLA-A*29:02 | CNDPFLGVYY | CNYPFLGVYY | 439 | 68 |
| HLA-A*29:02 | CNDPFLGVY | CNYPFLGVY | 566 | 93 |
| HLA-A*30:02 | CNDPFLGVY | CNYPFLGVY | 310 | 53 |
| HLA-C*03:02 | CNDPFLGVY | CNYPFLGVY | 2382 | 465 |
| HLA-A*30:02 | CNDPFLGVYY | CNYPFLGVYY | 549 | 121 |
| HLA-A*23:01 | CEFQFCNDPF | CEFQFCNYPF | 2202 | 488 |
| HLA-A*68:01 | DPFLGVYYHKNNK | YPFLGVYYHKNNK | 1317 | 340 |
| HLA-C*06:02 | FQFCNDPFL | FQFCNYPFL | 1653 | 434 |
| HLA-B*18:01 | FQFCNDPF | FQFCNYPF | 1795 | 488 |
| HLA-C*12:03 | FQFCNDPFL | FQFCNYPFL | 350 | 98 |
| HLA-A*29:02 | QFCNDPFLGVY | QFCNYPFLGVY | 192 | 57 |
| HLA-C*14:02 | FQFCNDPFL | FQFCNYPFL | 638 | 191 |
| HLA-C*07:02 | FQFCNDPFL | FQFCNYPFL | 1155 | 365 |
| HLA-A*29:02 | FCNDPFLGVY | FCNYPFLGVY | 587 | 189 |
| HLA-C*14:02 | EFQFCNDPF | EFQFCNYPF | 664 | 232 |
| HLA-B*15:01 | FCNDPFLGVY | FCNYPFLGVY | 887 | 330 |
| HLA-A*02:11 | FQFCNDPFLG | FQFCNYPFLG | 604 | 226 |
| HLA-B*18:01 | CEFQFCNDPF | CEFQFCNYPF | 102 | 39 |
| HLA-C*03:02 | FQFCNDPF | FQFCNYPF | 1019 | 391 |
| HLA-A*30:02 | FCNDPFLGVY | FCNYPFLGVY | 408 | 158 |
| HLA-B*44:03 | CEFQFCNDPF | CEFQFCNYPF | 89 | 37 |
| HLA-A*02:06 | FCNDPFLGV | FCNYPFLGV | 116 | 272 |
| HLA-B*40:02 | CEFQFCNDPF | CEFQFCNYPF | 245 | 105 |
| HLA-A*02:11 | EFQFCNDPFL | EFQFCNYPFL | 509 | 221 |
| HLA-B*50:01 | CEFQFCNDP | CEFQFCNYP | 934 | 406 |
| HLA-B*45:01 | CEFQFCNDPF | CEFQFCNYPF | 1099 | 486 |
| HLA-A*02:06 | FQFCNDPFLG | FQFCNYPFLG | 207 | 92 |
| HLA-A*02:06 | EFQFCNDPFLGV | EFQFCNYPFLGV | 87 | 194 |
| HLA-C*12:02 | FQFCNDPFL | FQFCNYPFL | 315 | 142 |
| HLA-A*02:06 | FQFCNDPFLGVY | FQFCNYPFLGVY | 135 | 295 |
| HLA-A*29:02 | FQFCNDPFLGVY | FQFCNYPFLGVY | 388 | 179 |
| HLA-A*02:06 | EFQFCNDPFL | EFQFCNYPFL | 227 | 107 |
| HLA-B*45:01 | CEFQFCNDP | CEFQFCNYP | 303 | 145 |
| HLA-A*32:01 | KVCEFQFCNDPF | KVCEFQFCNYPF | 376 | 180 |
| HLA-C*16:01 | FQFCNDPFL | FQFCNYPFL | 473 | 230 |
| HLA-C*02:02 | FQFCNDPFL | FQFCNYPFL | 522 | 254 |
| HLA-C*02:10 | FQFCNDPFL | FQFCNYPFL | 522 | 254 |
| HLA-B*40:06 | CEFQFCNDP | CEFQFCNYP | 782 | 384 |
| HLA-A*30:02 | QFCNDPFLGVY | QFCNYPFLGVY | 678 | 334 |
| HLA-A*02:02 | EFQFCNDPFLGV | EFQFCNYPFLGV | 477 | 964 |
| HLA-B*40:02 | FQFCNDPFL | FQFCNYPFL | 662 | 329 |
| HLA-A*02:11 | FQFCNDPFL | FQFCNYPFL | 6 | 3 |
| HLA-B*44:02 | CEFQFCNDPF | CEFQFCNYPF | 126 | 63 |
R190S
Reference NCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRD
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Mutated NCTFEYVSQPFLMDLEGKQGNFKNLSEFVFKNIDGYFKIYSKHTPINLVRD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*31:01 | KQGNFKNLR | KQGNFKNLS | 17 | 20961 |
| HLA-A*01:01 | LREFVFKNIDGY | LSEFVFKNIDGY | 14233 | 179 |
| HLA-A*31:01 | GKQGNFKNLR | GKQGNFKNLS | 475 | 32767 |
| HLA-A*02:01 | NLREFVFKNI | NLSEFVFKNI | 798 | 36 |
| HLA-A*02:02 | NLREFVFKNI | NLSEFVFKNI | 275 | 14 |
| HLA-A*02:06 | NLREFVFKNI | NLSEFVFKNI | 2450 | 205 |
| HLA-A*02:11 | KNLREFVFKNI | KNLSEFVFKNI | 3605 | 365 |
| HLA-A*02:11 | NLREFVFKNI | NLSEFVFKNI | 76 | 8 |
| HLA-A*68:02 | NLREFVFKNI | NLSEFVFKNI | 3406 | 369 |
| HLA-B*18:01 | REFVFKNIDGY | SEFVFKNIDGY | 1577 | 297 |
| HLA-B*15:01 | KQGNFKNLREF | KQGNFKNLSEF | 516 | 120 |
| HLA-B*44:03 | REFVFKNIDGY | SEFVFKNIDGY | 489 | 134 |
| HLA-B*44:02 | REFVFKNIDGY | SEFVFKNIDGY | 915 | 274 |
| HLA-B*15:03 | NFKNLREFVF | NFKNLSEFVF | 746 | 233 |
| HLA-B*44:03 | REFVFKNIDGYF | SEFVFKNIDGYF | 599 | 188 |
| HLA-B*44:02 | REFVFKNIDGYF | SEFVFKNIDGYF | 916 | 295 |
| HLA-B*40:02 | REFVFKNIDGYFKI | SEFVFKNIDGYFKI | 272 | 807 |
| HLA-A*30:02 | REFVFKNIDGY | SEFVFKNIDGY | 485 | 1323 |
| HLA-B*40:02 | REFVFKNIDGYF | SEFVFKNIDGYF | 254 | 675 |
| HLA-B*15:03 | REFVFKNIDGYF | SEFVFKNIDGYF | 402 | 1041 |
| HLA-B*15:03 | GKQGNFKNLREF | GKQGNFKNLSEF | 548 | 228 |
| HLA-B*15:03 | REFVFKNIDGY | SEFVFKNIDGY | 494 | 1162 |
| HLA-A*24:02 | NFKNLREFVF | NFKNLSEFVF | 786 | 356 |
| HLA-B*15:03 | GNFKNLREF | GNFKNLSEF | 136 | 62 |
| HLA-A*23:01 | NFKNLREFVF | NFKNLSEFVF | 405 | 200 |
| HLA-B*15:03 | KQGNFKNLREF | KQGNFKNLSEF | 42 | 21 |
K417T
Reference FTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLD
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Mutated FTNVYADSFVIRGDEVRQIAPGQTGTIADYNYKLPDDFTGCVIAWNSNNLD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | GKIADYNYK | GTIADYNYK | 8565 | 6 |
| HLA-A*03:01 | RQIAPGQTGK | RQIAPGQTGT | 49 | 23113 |
| HLA-A*68:01 | GKIADYNYK | GTIADYNYK | 12087 | 32 |
| HLA-A*03:02 | GKIADYNYK | GTIADYNYK | 14291 | 44 |
| HLA-A*11:01 | RQIAPGQTGK | RQIAPGQTGT | 103 | 30673 |
| HLA-A*03:02 | RQIAPGQTGK | RQIAPGQTGT | 123 | 25727 |
| HLA-A*11:01 | QIAPGQTGK | QIAPGQTGT | 165 | 32767 |
| HLA-A*68:01 | EVRQIAPGQTGK | EVRQIAPGQTGT | 144 | 27700 |
| HLA-A*68:01 | QIAPGQTGK | QIAPGQTGT | 136 | 20478 |
| HLA-A*03:01 | GKIADYNYK | GTIADYNYK | 15362 | 118 |
| HLA-A*03:02 | QIAPGQTGK | QIAPGQTGT | 278 | 32767 |
| HLA-A*11:01 | GKIADYNYKL | GTIADYNYKL | 30153 | 269 |
| HLA-A*03:01 | QIAPGQTGK | QIAPGQTGT | 296 | 32213 |
| HLA-A*11:01 | APGQTGKIADYNYK | APGQTGTIADYNYK | 15790 | 166 |
| HLA-A*68:01 | TGKIADYNYK | TGTIADYNYK | 957 | 13 |
| HLA-A*11:01 | TGKIADYNYK | TGTIADYNYK | 1113 | 19 |
| HLA-A*68:02 | KIADYNYKL | TIADYNYKL | 1274 | 34 |
| HLA-A*30:01 | GKIADYNYK | GTIADYNYK | 5364 | 151 |
| HLA-A*32:01 | KIADYNYKL | TIADYNYKL | 58 | 1664 |
| HLA-A*31:01 | GKIADYNYK | GTIADYNYK | 11055 | 392 |
| HLA-A*30:01 | RQIAPGQTGK | RQIAPGQTGT | 275 | 7151 |
| HLA-A*11:01 | GQTGKIADYNYK | GQTGTIADYNYK | 1342 | 63 |
| HLA-A*03:02 | TGKIADYNYK | TGTIADYNYK | 3525 | 241 |
| HLA-C*01:02 | IAPGQTGKI | IAPGQTGTI | 529 | 115 |
| HLA-A*03:02 | KIADYNYK | TIADYNYK | 476 | 2050 |
| HLA-A*30:01 | TGKIADYNYK | TGTIADYNYK | 292 | 948 |
| HLA-C*12:03 | IAPGQTGKI | IAPGQTGTI | 806 | 253 |
| HLA-A*02:01 | KIADYNYKL | TIADYNYKL | 23 | 69 |
| HLA-A*02:06 | KIADYNYKL | TIADYNYKL | 20 | 51 |
| HLA-A*30:02 | GQTGKIADY | GQTGTIADY | 337 | 135 |
| HLA-A*02:11 | KIADYNYKL | TIADYNYKL | 5 | 12 |
| HLA-A*01:01 | QTGKIADYNY | QTGTIADYNY | 580 | 276 |
| HLA-B*07:02 | APGQTGKIA | APGQTGTIA | 507 | 248 |
E484K
Reference SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
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Mutated SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:02 | VEGFNCYFPL | VKGFNCYFPL | 31 | 7011 |
| HLA-B*40:01 | VEGFNCYFPL | VKGFNCYFPL | 76 | 15210 |
| HLA-A*11:01 | GSTPCNGVE | GSTPCNGVK | 32767 | 444 |
| HLA-A*68:02 | EGFNCYFPL | KGFNCYFPL | 117 | 3877 |
| HLA-A*30:01 | EGFNCYFPL | KGFNCYFPL | 5413 | 164 |
| HLA-A*32:01 | EGFNCYFPL | KGFNCYFPL | 6548 | 210 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQSY | 1806 | 72 |
| HLA-B*15:01 | GVEGFNCYF | GVKGFNCYF | 3125 | 132 |
| HLA-A*31:01 | EGFNCYFPL | KGFNCYFPL | 4227 | 226 |
| HLA-B*15:03 | EGFNCYFPL | KGFNCYFPL | 2980 | 212 |
| HLA-A*02:11 | EGFNCYFPL | KGFNCYFPL | 2508 | 259 |
| HLA-B*15:03 | GVEGFNCYF | GVKGFNCYF | 1657 | 199 |
| HLA-A*02:06 | EGFNCYFPL | KGFNCYFPL | 1266 | 169 |
| HLA-B*35:01 | TPCNGVEGF | TPCNGVKGF | 319 | 1754 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQSY | 1081 | 208 |
| HLA-B*35:01 | NGVEGFNCY | NGVKGFNCY | 346 | 1439 |
| HLA-C*16:01 | EGFNCYFPL | KGFNCYFPL | 1861 | 498 |
| HLA-B*15:03 | VEGFNCYFPL | VKGFNCYFPL | 943 | 280 |
N501Y
Reference GSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*29:02 | QSYGFQPTN | QSYGFQPTY | 26152 | 24 |
| HLA-A*29:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 19514 | 25 |
| HLA-B*15:01 | LQSYGFQPTN | LQSYGFQPTY | 6135 | 8 |
| HLA-B*35:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8715 | 15 |
| HLA-C*03:02 | QSYGFQPTN | QSYGFQPTY | 12903 | 23 |
| HLA-B*15:03 | LQSYGFQPTN | LQSYGFQPTY | 2045 | 4 |
| HLA-B*15:03 | QSYGFQPTN | QSYGFQPTY | 6543 | 13 |
| HLA-C*16:01 | QSYGFQPTN | QSYGFQPTY | 10868 | 27 |
| HLA-B*35:01 | QSYGFQPTN | QSYGFQPTY | 24615 | 71 |
| HLA-C*12:03 | QSYGFQPTN | QSYGFQPTY | 12696 | 38 |
| HLA-B*15:03 | PLQSYGFQPTN | PLQSYGFQPTY | 22802 | 80 |
| HLA-C*14:02 | TNGVGYQPY | TYGVGYQPY | 5468 | 21 |
| HLA-A*29:02 | LQSYGFQPTN | LQSYGFQPTY | 28458 | 133 |
| HLA-C*03:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 23840 | 113 |
| HLA-C*03:02 | LQSYGFQPTN | LQSYGFQPTY | 23421 | 125 |
| HLA-A*30:02 | LQSYGFQPTN | LQSYGFQPTY | 7508 | 42 |
| HLA-A*30:02 | QSYGFQPTN | QSYGFQPTY | 5297 | 30 |
| HLA-B*15:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 20159 | 137 |
| HLA-C*03:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 27639 | 198 |
| HLA-A*29:02 | CYFPLQSYGFQPTN | CYFPLQSYGFQPTY | 27182 | 196 |
| HLA-C*14:02 | SYGFQPTN | SYGFQPTY | 20230 | 162 |
| HLA-A*29:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 32767 | 264 |
| HLA-B*15:01 | QSYGFQPTN | QSYGFQPTY | 21529 | 195 |
| HLA-C*14:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18230 | 169 |
| HLA-B*15:03 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 22168 | 206 |
| HLA-A*11:01 | QSYGFQPTN | QSYGFQPTY | 22068 | 241 |
| HLA-C*16:01 | LQSYGFQPTN | LQSYGFQPTY | 27149 | 326 |
| HLA-C*12:02 | QSYGFQPTN | QSYGFQPTY | 21029 | 267 |
| HLA-B*15:01 | PLQSYGFQPTN | PLQSYGFQPTY | 32767 | 449 |
| HLA-B*35:01 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 28375 | 395 |
| HLA-C*12:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27893 | 431 |
| HLA-C*16:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27733 | 434 |
| HLA-C*02:02 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-C*02:10 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-A*30:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18743 | 310 |
| HLA-B*15:03 | LQSYGFQPTNG | LQSYGFQPTYG | 6387 | 109 |
| HLA-B*53:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8451 | 151 |
| HLA-C*12:03 | LQSYGFQPTN | LQSYGFQPTY | 26641 | 480 |
| HLA-A*32:01 | QSYGFQPTN | QSYGFQPTY | 16619 | 311 |
| HLA-A*30:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 21475 | 466 |
| HLA-C*03:02 | NGVGYQPY | YGVGYQPY | 10463 | 451 |
| HLA-B*57:01 | QSYGFQPTN | QSYGFQPTY | 4070 | 215 |
| HLA-B*58:01 | QSYGFQPTN | QSYGFQPTY | 1532 | 83 |
| HLA-A*02:11 | NGVGYQPYRV | YGVGYQPYRV | 5989 | 327 |
| HLA-B*57:01 | LQSYGFQPTN | LQSYGFQPTY | 3839 | 244 |
| HLA-B*58:01 | LQSYGFQPTN | LQSYGFQPTY | 1885 | 155 |
| HLA-B*15:03 | NGVGYQPY | YGVGYQPY | 4677 | 467 |
| HLA-A*31:01 | TNGVGYQPYR | TYGVGYQPYR | 811 | 84 |
| HLA-A*33:03 | TNGVGYQPYR | TYGVGYQPYR | 573 | 62 |
| HLA-A*29:02 | TNGVGYQPY | TYGVGYQPY | 2031 | 237 |
| HLA-A*29:02 | PTNGVGYQPY | PTYGVGYQPY | 1211 | 177 |
| HLA-A*02:11 | YGFQPTNGV | YGFQPTYGV | 307 | 49 |
| HLA-A*02:01 | YGFQPTNGV | YGFQPTYGV | 2160 | 459 |
| HLA-A*68:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1073 | 242 |
| HLA-A*02:11 | LQSYGFQPTNGV | LQSYGFQPTYGV | 1446 | 396 |
| HLA-A*31:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1377 | 406 |
| HLA-A*02:06 | YGFQPTNGV | YGFQPTYGV | 174 | 52 |
| HLA-A*33:03 | PTNGVGYQPYR | PTYGVGYQPYR | 1345 | 407 |
| HLA-A*02:02 | YGFQPTNGV | YGFQPTYGV | 868 | 287 |
| HLA-A*02:06 | QSYGFQPTNGV | QSYGFQPTYGV | 1121 | 407 |
| HLA-A*68:02 | YGFQPTNGV | YGFQPTYGV | 73 | 28 |
| HLA-A*68:02 | QSYGFQPTNGV | QSYGFQPTYGV | 122 | 48 |
| HLA-B*15:01 | GFQPTNGVGY | GFQPTYGVGY | 774 | 311 |
| HLA-A*29:02 | YGFQPTNGVGY | YGFQPTYGVGY | 1158 | 486 |
| HLA-A*31:01 | NGVGYQPYR | YGVGYQPYR | 438 | 185 |
| HLA-A*02:06 | LQSYGFQPTNGV | LQSYGFQPTYGV | 249 | 111 |
| HLA-A*30:02 | PTNGVGYQPY | PTYGVGYQPY | 465 | 210 |
| HLA-B*15:03 | FQPTNGVGY | FQPTYGVGY | 63 | 29 |
| HLA-A*02:02 | LQSYGFQPTNGV | LQSYGFQPTYGV | 594 | 280 |
| HLA-B*15:03 | GFQPTNGVGY | GFQPTYGVGY | 769 | 363 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
H655Y
Reference TPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TPTWRVYSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTNS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:01 | RAGCLIGAEH | RAGCLIGAEY | 7377 | 156 |
| HLA-A*30:02 | RAGCLIGAEH | RAGCLIGAEY | 1881 | 43 |
| HLA-B*15:01 | FQTRAGCLIGAEH | FQTRAGCLIGAEY | 7512 | 178 |
| HLA-B*15:03 | FQTRAGCLIGAEH | FQTRAGCLIGAEY | 5076 | 125 |
| HLA-A*30:02 | AGCLIGAEH | AGCLIGAEY | 5331 | 179 |
| HLA-B*15:03 | RAGCLIGAEH | RAGCLIGAEY | 5254 | 179 |
| HLA-A*30:02 | QTRAGCLIGAEH | QTRAGCLIGAEY | 8425 | 342 |
| HLA-B*15:03 | AGCLIGAEH | AGCLIGAEY | 9025 | 461 |
| HLA-A*02:11 | HVNNSYECDIPI | YVNNSYECDIPI | 1593 | 109 |
| HLA-A*02:01 | HVNNSYECDIPI | YVNNSYECDIPI | 2384 | 226 |
| HLA-A*02:06 | HVNNSYECDIPI | YVNNSYECDIPI | 837 | 92 |
| HLA-A*02:02 | HVNNSYECDIPI | YVNNSYECDIPI | 1466 | 187 |
| HLA-A*02:11 | CLIGAEHV | CLIGAEYV | 983 | 221 |
| HLA-B*15:03 | AEHVNNSY | AEYVNNSY | 986 | 267 |
| HLA-B*18:01 | AEHVNNSY | AEYVNNSY | 902 | 286 |
T1027I
Reference QSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated QSLQTYVTQQLIRAAEIRASANLAAIKMSECVLGQSKRVDFCGKGYHLMSF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*03:03 | RASANLAAT | RASANLAAI | 4611 | 62 |
| HLA-C*03:04 | RASANLAAT | RASANLAAI | 4611 | 62 |
| HLA-A*32:01 | RASANLAAT | RASANLAAI | 22711 | 434 |
| HLA-C*12:03 | RASANLAAT | RASANLAAI | 2694 | 60 |
| HLA-C*17:01 | RASANLAAT | RASANLAAI | 11235 | 278 |
| HLA-C*15:02 | RASANLAAT | RASANLAAI | 6895 | 180 |
| HLA-C*16:01 | RASANLAAT | RASANLAAI | 924 | 25 |
| HLA-B*40:01 | AEIRASANLAAT | AEIRASANLAAI | 8508 | 344 |
| HLA-C*03:02 | RASANLAAT | RASANLAAI | 2899 | 129 |
| HLA-B*58:01 | RASANLAAT | RASANLAAI | 5883 | 333 |
| HLA-B*15:03 | RASANLAAT | RASANLAAI | 3151 | 184 |
| HLA-B*44:02 | AEIRASANLAAT | AEIRASANLAAI | 6113 | 368 |
| HLA-C*12:02 | RASANLAAT | RASANLAAI | 5044 | 305 |
| HLA-B*40:02 | AEIRASANLAAT | AEIRASANLAAI | 1973 | 232 |
| HLA-A*02:06 | RASANLAAT | RASANLAAI | 2857 | 336 |
| HLA-A*30:01 | ATKMSECVL | AIKMSECVL | 173 | 1150 |
| HLA-C*16:01 | ATKMSECVL | AIKMSECVL | 476 | 2866 |
| HLA-A*30:01 | ATKMSECVLGQSK | AIKMSECVLGQSK | 464 | 2739 |
| HLA-A*30:01 | RASANLAAT | RASANLAAI | 1238 | 321 |
| HLA-A*02:02 | NLAATKMSECVL | NLAAIKMSECVL | 877 | 285 |
| HLA-A*02:02 | NLAATKMSECV | NLAAIKMSECV | 702 | 236 |
| HLA-A*02:11 | NLAATKMSECV | NLAAIKMSECV | 647 | 232 |
V1176F
Reference ELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQ
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated ELDKYFKNHTSPDVDLGDISGINASFVNIQKEIDRLNEVAKNLNESLIDLQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | DISGINASV | DISGINASF | 10 | 1289 |
| HLA-A*02:11 | GDISGINASV | GDISGINASF | 238 | 15161 |
| HLA-C*14:02 | SVVNIQKEI | SFVNIQKEI | 6508 | 124 |
| HLA-A*02:02 | GDISGINASV | GDISGINASF | 71 | 3625 |
| HLA-A*02:11 | LGDISGINASV | LGDISGINASF | 446 | 19525 |
| HLA-A*68:02 | DLGDISGINASV | DLGDISGINASF | 362 | 15753 |
| HLA-B*15:01 | GDISGINASV | GDISGINASF | 15806 | 370 |
| HLA-A*02:02 | LGDISGINASV | LGDISGINASF | 208 | 8728 |
| HLA-B*35:01 | DISGINASV | DISGINASF | 16065 | 447 |
| HLA-A*68:02 | GDISGINASV | GDISGINASF | 453 | 15954 |
| HLA-A*02:02 | VDLGDISGINASV | VDLGDISGINASF | 413 | 11912 |
| HLA-A*68:01 | SVVNIQKEIDR | SFVNIQKEIDR | 288 | 5363 |
| HLA-A*02:02 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 2908 | 166 |
| HLA-A*25:01 | DISGINASV | DISGINASF | 3057 | 204 |
| HLA-A*02:06 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 871 | 59 |
| HLA-A*26:01 | DISGINASV | DISGINASF | 1865 | 129 |
| HLA-A*02:11 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 2044 | 152 |
| HLA-A*68:01 | VVNIQKEIDR | FVNIQKEIDR | 1917 | 242 |
| HLA-A*02:06 | GINASVVNI | GINASFVNI | 1251 | 237 |
| HLA-A*68:01 | SGINASVVNIQK | SGINASFVNIQK | 913 | 194 |
| HLA-A*68:01 | NASVVNIQKEIDR | NASFVNIQKEIDR | 127 | 30 |
| HLA-A*68:01 | INASVVNIQK | INASFVNIQK | 249 | 62 |
| HLA-A*11:01 | ASVVNIQK | ASFVNIQK | 1597 | 479 |
| HLA-A*68:02 | DISGINASVV | DISGINASFV | 59 | 19 |
| HLA-A*03:02 | NASVVNIQK | NASFVNIQK | 949 | 325 |
| HLA-A*11:01 | NASVVNIQK | NASFVNIQK | 235 | 83 |
| HLA-A*11:01 | SGINASVVNIQK | SGINASFVNIQK | 411 | 150 |
| HLA-A*11:01 | INASVVNIQK | INASFVNIQK | 270 | 101 |
| HLA-A*03:02 | INASVVNIQK | INASFVNIQK | 840 | 335 |
| HLA-A*11:01 | ISGINASVVNIQK | ISGINASFVNIQK | 1056 | 450 |
| HLA-A*68:01 | NASVVNIQK | NASFVNIQK | 13 | 6 |
| HLA-C*16:01 | ISGINASVV | ISGINASFV | 368 | 787 |
N protein
P80R
Reference ALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated ALTQHGKEDLKFPRGQGVPINTNSSRDDQIGYYRRATRRIRGGDGKMKDLS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*35:01 | SPDDQIGYY | SRDDQIGYY | 101 | 17780 |
| HLA-A*68:01 | GVPINTNSSP | GVPINTNSSR | 32767 | 293 |
| HLA-A*31:01 | RGQGVPINTNSSP | RGQGVPINTNSSR | 32767 | 464 |
| HLA-A*31:01 | PDDQIGYYRRATRR | RDDQIGYYRRATRR | 9418 | 219 |
| HLA-A*31:01 | PDDQIGYYRRATR | RDDQIGYYRRATR | 7216 | 180 |
| HLA-A*31:01 | SSPDDQIGYYR | SSRDDQIGYYR | 625 | 27 |
| HLA-A*30:02 | SSPDDQIGY | SSRDDQIGY | 2069 | 139 |
| HLA-B*15:03 | SSPDDQIGY | SSRDDQIGY | 4130 | 376 |
| HLA-C*03:02 | SSPDDQIGY | SSRDDQIGY | 2626 | 388 |
| HLA-A*33:03 | SSPDDQIGYYR | SSRDDQIGYYR | 890 | 132 |
| HLA-C*16:01 | SSPDDQIGY | SSRDDQIGY | 1829 | 337 |
| HLA-A*30:02 | SSPDDQIGYY | SSRDDQIGYY | 247 | 48 |
| HLA-A*31:01 | SSPDDQIGYYRR | SSRDDQIGYYRR | 481 | 108 |
| HLA-A*68:01 | NSSPDDQIGYYRR | NSSRDDQIGYYRR | 487 | 1247 |
| HLA-A*31:01 | NTNSSPDDQIGYYR | NTNSSRDDQIGYYR | 356 | 175 |
203-204 RG->KR
Reference GGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | NSTPGSSRG | NSTPGSSKR | 32767 | 34 |
| HLA-A*33:03 | NSTPGSSRG | NSTPGSSKR | 32767 | 300 |
| HLA-B*15:03 | SRGTSPARM | SKRTSPARM | 2669 | 106 |
| HLA-A*31:01 | RNSTPGSSRG | RNSTPGSSKR | 7013 | 340 |
| HLA-A*30:01 | SSRNSTPGSSR | SSRNSTPGSSK | 356 | 20 |
| HLA-A*30:01 | GTSPARMAG | RTSPARMAG | 493 | 30 |
| HLA-A*31:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 3757 | 231 |
| HLA-A*30:01 | GTSPARMAGNGG | RTSPARMAGNGG | 6051 | 411 |
| HLA-A*31:01 | SSRNSTPGSSR | SSRNSTPGSSK | 127 | 1853 |
| HLA-A*30:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 4255 | 353 |
| HLA-A*30:01 | RNSTPGSSR | RNSTPGSSK | 2120 | 188 |
| HLA-A*31:01 | RNSTPGSSR | RNSTPGSSK | 123 | 1335 |
| HLA-A*03:01 | SSRNSTPGSSR | SSRNSTPGSSK | 4753 | 442 |
| HLA-A*11:01 | SSRNSTPGSSR | SSRNSTPGSSK | 2231 | 263 |
| HLA-A*68:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 270 | 1385 |
| HLA-A*30:01 | GSSRGTSPA | GSSKRTSPA | 555 | 145 |
| HLA-A*30:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 1625 | 469 |
| HLA-A*30:01 | SSRGTSPARMAG | SSKRTSPARMAG | 449 | 1031 |
NS3 protein
S253P
Reference VTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL
||||||||||||||||||||||||| ||||||||||||||||||||||
Mutated VTFFIYNKIVDEPEEHVQIHTIDGSPGVVNPVMEPIYDEPTTTTSVPL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*42:01 | SSGVVNPVM | SPGVVNPVM | 7497 | 28 |
| HLA-B*07:02 | SSGVVNPVM | SPGVVNPVM | 13017 | 70 |
| HLA-B*35:01 | SSGVVNPVM | SPGVVNPVM | 6921 | 73 |
| HLA-B*42:01 | SSGVVNPV | SPGVVNPV | 22475 | 317 |
| HLA-B*35:03 | SSGVVNPVM | SPGVVNPVM | 13571 | 355 |
| HLA-C*16:01 | SSGVVNPVM | SPGVVNPVM | 119 | 4324 |
| HLA-C*15:02 | GSSGVVNPV | GSPGVVNPV | 410 | 2450 |
| HLA-A*68:02 | DGSSGVVNPV | DGSPGVVNPV | 20 | 98 |
| HLA-A*02:01 | HTIDGSSGV | HTIDGSPGV | 658 | 140 |
| HLA-A*02:02 | GSSGVVNPV | GSPGVVNPV | 173 | 736 |
| HLA-A*68:02 | GSSGVVNPV | GSPGVVNPV | 52 | 214 |
| HLA-A*68:02 | VQIHTIDGSSGV | VQIHTIDGSPGV | 242 | 782 |
| HLA-A*02:02 | TIDGSSGVVNPV | TIDGSPGVVNPV | 314 | 1001 |
| HLA-A*02:11 | HTIDGSSGV | HTIDGSPGV | 81 | 26 |
| HLA-A*02:06 | IHTIDGSSGV | IHTIDGSPGV | 263 | 90 |
| HLA-A*68:02 | IHTIDGSSGV | IHTIDGSPGV | 40 | 114 |
| HLA-A*02:06 | HTIDGSSGV | HTIDGSPGV | 33 | 12 |
| HLA-A*68:02 | QIHTIDGSSGV | QIHTIDGSPGV | 118 | 318 |
| HLA-A*68:02 | TIDGSSGVVNPV | TIDGSPGVVNPV | 61 | 163 |
| HLA-A*68:02 | HVQIHTIDGSSGV | HVQIHTIDGSPGV | 63 | 159 |
| HLA-A*68:02 | EHVQIHTIDGSSGV | EHVQIHTIDGSPGV | 61 | 153 |
| HLA-A*02:06 | HTIDGSSGVV | HTIDGSPGVV | 589 | 238 |
| HLA-A*02:11 | IHTIDGSSGV | IHTIDGSPGV | 681 | 279 |
| HLA-A*26:01 | HTIDGSSGV | HTIDGSPGV | 149 | 361 |
| HLA-A*02:06 | VQIHTIDGSSGV | VQIHTIDGSPGV | 121 | 50 |
| HLA-A*02:11 | VQIHTIDGSSGV | VQIHTIDGSPGV | 931 | 444 |
| HLA-A*02:06 | GSSGVVNPV | GSPGVVNPV | 87 | 174 |
NS8 protein
E92K
Reference SKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRCSFYEDFLEYHDVRVV
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated SKSPIQYIDIGNYTVSCLPFTINCQKPKLGSLVVRCSFYEDFLEYHDVRVV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | TVSCLPFTINCQE | TVSCLPFTINCQK | 13170 | 48 |
| HLA-A*11:01 | TVSCLPFTINCQE | TVSCLPFTINCQK | 31119 | 132 |
| HLA-A*68:01 | YTVSCLPFTINCQE | YTVSCLPFTINCQK | 14537 | 66 |
| HLA-A*03:02 | CLPFTINCQE | CLPFTINCQK | 18017 | 93 |
| HLA-A*03:01 | CLPFTINCQE | CLPFTINCQK | 22259 | 120 |
| HLA-A*03:02 | TVSCLPFTINCQE | TVSCLPFTINCQK | 31679 | 311 |
| HLA-A*11:01 | CLPFTINCQE | CLPFTINCQK | 28982 | 308 |
| HLA-A*11:01 | VSCLPFTINCQE | VSCLPFTINCQK | 28194 | 308 |
| HLA-A*68:01 | CLPFTINCQE | CLPFTINCQK | 15343 | 247 |
| HLA-A*68:01 | LPFTINCQE | LPFTINCQK | 17124 | 303 |
| HLA-B*07:02 | EPKLGSLVV | KPKLGSLVV | 685 | 18 |
| HLA-B*08:01 | CQEPKLGSL | CQKPKLGSL | 3079 | 81 |
| HLA-B*07:02 | EPKLGSLVVRCSF | KPKLGSLVVRCSF | 8372 | 301 |
| HLA-B*07:02 | EPKLGSLV | KPKLGSLV | 11398 | 438 |
| HLA-B*08:01 | NCQEPKLGSL | NCQKPKLGSL | 5460 | 397 |
| HLA-B*42:01 | EPKLGSLV | KPKLGSLV | 3548 | 271 |
| HLA-B*42:01 | EPKLGSLVVRCSF | KPKLGSLVVRCSF | 2752 | 283 |
| HLA-B*15:01 | CQEPKLGSL | CQKPKLGSL | 1956 | 239 |
| HLA-B*42:01 | EPKLGSLVV | KPKLGSLVV | 118 | 15 |
| HLA-A*02:02 | FTINCQEPKL | FTINCQKPKL | 140 | 765 |
| HLA-A*68:01 | PFTINCQEPK | PFTINCQKPK | 130 | 626 |
| HLA-A*68:02 | FTINCQEPKL | FTINCQKPKL | 113 | 534 |
| HLA-A*68:01 | FTINCQEPKL | FTINCQKPKL | 395 | 1765 |
| HLA-A*02:06 | FTINCQEPKL | FTINCQKPKL | 134 | 511 |
| HLA-B*15:03 | CQEPKLGSL | CQKPKLGSL | 432 | 137 |
| HLA-A*31:01 | CQEPKLGSLVVR | CQKPKLGSLVVR | 986 | 334 |
| HLA-A*68:01 | FTINCQEPK | FTINCQKPK | 7 | 15 |
| HLA-A*03:02 | FTINCQEPK | FTINCQKPK | 121 | 245 |
NS9b protein
Q77E
Reference LSLNMARKTLNSLEDKAFQLTPIAVQMTKLATTEELPDEFVVVTVK
||||||||||||||||||||||||| ||||||||||||||||||||
Mutated LSLNMARKTLNSLEDKAFQLTPIAVEMTKLATTEELPDEFVVVTVK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:06 | VQMTKLATT | VEMTKLATT | 120 | 10924 |
| HLA-B*40:01 | VQMTKLATTEEL | VEMTKLATTEEL | 11510 | 296 |
| HLA-B*45:01 | VQMTKLATT | VEMTKLATT | 11925 | 346 |
| HLA-B*40:02 | VQMTKLATT | VEMTKLATT | 6271 | 199 |
| HLA-B*45:01 | AVQMTKLATT | AVEMTKLATT | 11108 | 369 |
| HLA-B*40:02 | VQMTKLATTEEL | VEMTKLATTEEL | 10699 | 438 |
| HLA-B*15:03 | VQMTKLATT | VEMTKLATT | 344 | 6412 |
| HLA-A*03:01 | LTPIAVQMTK | LTPIAVEMTK | 105 | 337 |
| HLA-B*07:02 | TPIAVQMTKL | TPIAVEMTKL | 278 | 648 |
| HLA-A*03:01 | KAFQLTPIAVQMTK | KAFQLTPIAVEMTK | 425 | 895 |
| HLA-B*42:01 | TPIAVQMTKL | TPIAVEMTKL | 86 | 173 |
| HLA-A*11:01 | LTPIAVQMTK | LTPIAVEMTK | 23 | 46 |
NS9c protein
49-50 VG->LN
Reference EAAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||||
Mutated EAAVKPLLVPHHVVATVQEIQLQAALNELLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | AVGELLLLEWL | ALNELLLLEWL | 3883 | 38 |
| HLA-A*02:02 | AVGELLLLEWL | ALNELLLLEWL | 3830 | 57 |
| HLA-A*02:11 | AVGELLLL | ALNELLLL | 10065 | 162 |
| HLA-A*02:01 | AVGELLLLEWL | ALNELLLLEWL | 6165 | 133 |
| HLA-A*02:02 | AVGELLLL | ALNELLLL | 7231 | 159 |
| HLA-A*02:11 | AVGELLLLEWLA | ALNELLLLEWLA | 837 | 32 |
| HLA-A*02:02 | AVGELLLLEWLA | ALNELLLLEWLA | 2812 | 125 |
| HLA-B*18:01 | GELLLLEWLAM | NELLLLEWLAM | 4979 | 247 |
| HLA-A*02:11 | AVGELLLLEWLAM | ALNELLLLEWLAM | 8089 | 438 |
| HLA-A*02:01 | AVGELLLLEWLA | ALNELLLLEWLA | 2242 | 175 |
| HLA-B*40:01 | VQEIQLQAAV | VQEIQLQAAL | 747 | 59 |
| HLA-B*40:01 | ATVQEIQLQAAV | ATVQEIQLQAAL | 1322 | 126 |
| HLA-B*40:01 | TVQEIQLQAAV | TVQEIQLQAAL | 2519 | 242 |
| HLA-B*18:01 | GELLLLEW | NELLLLEW | 3915 | 401 |
| HLA-B*45:01 | QEIQLQAAV | QEIQLQAAL | 51 | 496 |
| HLA-A*02:06 | QLQAAVGEL | QLQAALNEL | 2794 | 314 |
| HLA-B*15:01 | VQEIQLQAAV | VQEIQLQAAL | 1183 | 208 |
| HLA-A*02:11 | QLQAAVGEL | QLQAALNEL | 120 | 22 |
| HLA-B*15:03 | VQEIQLQAAV | VQEIQLQAAL | 975 | 183 |
| HLA-A*02:01 | QLQAAVGEL | QLQAALNEL | 1290 | 245 |
| HLA-B*50:01 | QEIQLQAAV | QEIQLQAAL | 260 | 1357 |
| HLA-B*40:01 | GELLLLEWL | NELLLLEWL | 28 | 140 |
| HLA-B*40:01 | QEIQLQAAV | QEIQLQAAL | 42 | 9 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAALNEL | 338 | 75 |
| HLA-B*40:06 | QEIQLQAAV | QEIQLQAAL | 210 | 901 |
| HLA-A*02:02 | QLQAAVGEL | QLQAALNEL | 92 | 23 |
| HLA-B*40:02 | GELLLLEWL | NELLLLEWL | 52 | 204 |
| HLA-B*40:02 | GELLLLEWLAM | NELLLLEWLAM | 201 | 772 |
| HLA-C*03:03 | AAVGELLLL | AALNELLLL | 107 | 409 |
| HLA-C*03:04 | AAVGELLLL | AALNELLLL | 107 | 409 |
| HLA-A*02:01 | QLQAAVGELLLL | QLQAALNELLLL | 748 | 203 |
| HLA-A*02:01 | IQLQAAVGEL | IQLQAALNEL | 1138 | 318 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAALNEL | 170 | 49 |
| HLA-A*02:06 | VQEIQLQAAV | VQEIQLQAAL | 286 | 958 |
| HLA-B*40:02 | ATVQEIQLQAAV | ATVQEIQLQAAL | 575 | 174 |
| HLA-C*12:03 | AAVGELLLL | AALNELLLL | 229 | 705 |
| HLA-B*40:02 | VQEIQLQAAV | VQEIQLQAAL | 270 | 89 |
| HLA-B*57:01 | AVGELLLLEW | ALNELLLLEW | 432 | 1281 |
| HLA-B*40:02 | TVQEIQLQAAV | TVQEIQLQAAL | 1139 | 394 |
| HLA-A*02:02 | AVGELLLLEWLAMA | ALNELLLLEWLAMA | 1165 | 403 |
| HLA-C*03:02 | AAVGELLLL | AALNELLLL | 269 | 710 |
| HLA-B*58:01 | AVGELLLLEW | ALNELLLLEW | 196 | 513 |
| HLA-A*02:11 | QLQAAVGELLLL | QLQAALNELLLL | 563 | 217 |
| HLA-B*40:06 | GELLLLEWLA | NELLLLEWLA | 268 | 677 |
| HLA-B*40:02 | GELLLLEWLA | NELLLLEWLA | 475 | 1195 |
| HLA-B*58:01 | VGELLLLEW | LNELLLLEW | 330 | 796 |
| HLA-A*02:01 | GELLLLEWLAMA | NELLLLEWLAMA | 109 | 258 |
| HLA-A*02:01 | QLQAAVGELL | QLQAALNELL | 961 | 420 |
| HLA-B*18:01 | QEIQLQAAV | QEIQLQAAL | 316 | 141 |
| HLA-B*15:03 | LQAAVGELL | LQAALNELL | 25 | 54 |
| HLA-A*02:11 | GELLLLEWLAMA | NELLLLEWLAMA | 29 | 61 |
| HLA-B*15:01 | LQAAVGELLL | LQAALNELLL | 109 | 229 |
| HLA-A*02:02 | LQAAVGELL | LQAALNELL | 44 | 21 |
| HLA-A*02:02 | QLQAAVGELL | QLQAALNELL | 35 | 17 |
| HLA-B*15:01 | LQAAVGELL | LQAALNELL | 105 | 213 |
NSP3 protein
S370L
Reference RVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKP
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated RVCVDTVRTNVYLAVFDKNLYDKLVLSFLEMKSEKQVEQKIAEIPKEEVKP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | VSSFLEMKSEKQV | VLSFLEMKSEKQV | 6974 | 166 |
| HLA-A*02:11 | VSSFLEMKSEKQV | VLSFLEMKSEKQV | 9606 | 232 |
| HLA-B*08:01 | NLYDKLVS | NLYDKLVL | 11807 | 327 |
| HLA-A*02:11 | NLYDKLVS | NLYDKLVL | 5915 | 413 |
| HLA-A*02:11 | YDKLVSSFLEM | YDKLVLSFLEM | 216 | 2385 |
| HLA-A*02:02 | YDKLVSSFLEM | YDKLVLSFLEM | 366 | 3365 |
| HLA-A*02:01 | YDKLVSSFLEM | YDKLVLSFLEM | 363 | 3123 |
| HLA-A*03:02 | VSSFLEMKSEK | VLSFLEMKSEK | 1460 | 190 |
| HLA-A*02:06 | YDKLVSSFLEM | YDKLVLSFLEM | 489 | 3706 |
| HLA-A*02:11 | KLVSSFLEM | KLVLSFLEM | 12 | 77 |
| HLA-A*03:01 | VSSFLEMKSEK | VLSFLEMKSEK | 2465 | 391 |
| HLA-A*68:01 | LVSSFLEMK | LVLSFLEMK | 23 | 125 |
| HLA-A*68:01 | YDKLVSSFLEMK | YDKLVLSFLEMK | 207 | 1110 |
| HLA-A*02:02 | KLVSSFLEM | KLVLSFLEM | 58 | 309 |
| HLA-A*02:01 | KLVSSFLEM | KLVLSFLEM | 58 | 291 |
| HLA-A*03:01 | DKLVSSFLEMK | DKLVLSFLEMK | 215 | 1065 |
| HLA-A*03:01 | KLVSSFLEMKS | KLVLSFLEMKS | 470 | 2283 |
| HLA-A*02:06 | KLVSSFLEM | KLVLSFLEM | 46 | 223 |
| HLA-C*03:03 | KLVSSFLEM | KLVLSFLEM | 299 | 1350 |
| HLA-C*03:04 | KLVSSFLEM | KLVLSFLEM | 299 | 1350 |
| HLA-C*03:02 | KLVSSFLEM | KLVLSFLEM | 141 | 565 |
| HLA-A*68:01 | KLVSSFLEMK | KLVLSFLEMK | 497 | 1977 |
| HLA-A*68:01 | DKLVSSFLEMK | DKLVLSFLEMK | 191 | 757 |
| HLA-C*14:02 | KLVSSFLEM | KLVLSFLEM | 407 | 1572 |
| HLA-A*11:01 | SSFLEMKSEK | LSFLEMKSEK | 25 | 96 |
| HLA-A*68:01 | LVSSFLEMKSEK | LVLSFLEMKSEK | 399 | 1478 |
| HLA-A*11:01 | KLVSSFLEMKSEK | KLVLSFLEMKSEK | 399 | 1396 |
| HLA-C*14:02 | LYDKLVSSFLEM | LYDKLVLSFLEM | 307 | 1057 |
| HLA-A*03:01 | KLVSSFLEMKSEK | KLVLSFLEMKSEK | 80 | 273 |
| HLA-A*03:02 | KLVSSFLEMKSEK | KLVLSFLEMKSEK | 150 | 420 |
| HLA-A*30:01 | SSFLEMKSEK | LSFLEMKSEK | 247 | 617 |
| HLA-B*15:01 | KLVSSFLEM | KLVLSFLEM | 38 | 93 |
| HLA-B*15:03 | KLVSSFLEM | KLVLSFLEM | 12 | 29 |
| HLA-C*14:02 | LYDKLVSSF | LYDKLVLSF | 29 | 70 |
| HLA-A*23:01 | NLYDKLVSSF | NLYDKLVLSF | 760 | 320 |
| HLA-A*32:01 | KLVSSFLEM | KLVLSFLEM | 237 | 559 |
| HLA-C*14:02 | LYDKLVSSFL | LYDKLVLSFL | 231 | 502 |
| HLA-A*03:01 | KLVSSFLEMK | KLVLSFLEMK | 13 | 28 |
| HLA-A*03:02 | SSFLEMKSEK | LSFLEMKSEK | 232 | 490 |
| HLA-C*07:02 | LYDKLVSSF | LYDKLVLSF | 234 | 474 |
K977Q
Reference YMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGT
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Mutated YMGTLSYEQFKKGVQIPCTCGKQATQYLVQQESPFVMMSAPPAQYELKHGT
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | CTCGKQATK | CTCGKQATQ | 217 | 18007 |
| HLA-A*68:01 | CTCGKQATK | CTCGKQATQ | 128 | 7381 |
| HLA-A*03:02 | CTCGKQATK | CTCGKQATQ | 400 | 19435 |
| HLA-B*15:01 | TKYLVQQESPF | TQYLVQQESPF | 2003 | 83 |
| HLA-A*02:06 | TKYLVQQESPFV | TQYLVQQESPFV | 3595 | 354 |
| HLA-B*15:03 | TKYLVQQESPFVM | TQYLVQQESPFVM | 491 | 80 |
| HLA-B*15:03 | ATKYLVQQESPF | ATQYLVQQESPF | 1335 | 317 |
| HLA-B*15:03 | KYLVQQESPFVM | QYLVQQESPFVM | 253 | 1053 |
| HLA-B*15:03 | TKYLVQQESPF | TQYLVQQESPF | 65 | 16 |
| HLA-B*15:03 | KYLVQQESPF | QYLVQQESPF | 286 | 791 |
| HLA-B*15:01 | KYLVQQESPF | QYLVQQESPF | 95 | 262 |
| HLA-A*23:01 | KYLVQQESPFVM | QYLVQQESPFVM | 410 | 1103 |
| HLA-A*24:02 | KYLVQQESPF | QYLVQQESPF | 145 | 373 |
| HLA-A*23:01 | KYLVQQESPF | QYLVQQESPF | 98 | 247 |
NSP6 protein
SGF 106-108 deletion
Reference FNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FNMVYMPASWVMRIMTWLDMVDTSL‑‑‑KLKDCVMYASAVVLLILMTARTVYD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | FKLKDCVMYA | - | 8 | - |
| HLA-A*02:11 | FKLKDCVMYA | - | 12 | - |
| HLA-A*68:01 | MVDTSLSGFK | MVDTSLK | 13 | - |
| HLA-B*15:03 | FKLKDCVMY | - | 15 | - |
| HLA-A*11:01 | MVDTSLSGFK | MVDTSLK | 21 | - |
| HLA-A*11:01 | TSLSGFKLK | TSLKLK | 22 | - |
| HLA-A*03:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 25 |
| HLA-A*02:01 | FKLKDCVMYA | - | 27 | - |
| HLA-A*68:01 | DTSLSGFKLK | DTSLKLK | 33 | - |
| HLA-A*03:02 | MVDTSLSGFK | MVDTSLK | 35 | - |
| HLA-A*68:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 37 |
| HLA-A*03:01 | MVDTSLSGFK | MVDTSLK | 54 | - |
| HLA-A*03:02 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 59 |
| HLA-C*05:01 | MVDTSLSGF | MVDTSL | 64 | - |
| HLA-A*11:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 71 |
| HLA-A*02:06 | FKLKDCVMYA | - | 90 | - |
| HLA-A*30:01 | TSLSGFKLK | TSLKLK | 107 | - |
| HLA-A*02:02 | SGFKLKDCVMYA | - | 126 | - |
| HLA-A*11:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 128 |
| HLA-A*03:02 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 147 |
| HLA-C*08:02 | MVDTSLSGF | MVDTSL | 159 | - |
| HLA-A*03:02 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 168 |
| HLA-A*02:11 | SGFKLKDCVMYA | - | 200 | - |
| HLA-A*02:02 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 202 |
| HLA-A*01:01 | MVDTSLSGFKLKDCVMY | MVDTSLKLKDCVMY | - | 207 |
| HLA-C*03:02 | MVDTSLSGF | MVDTSL | 211 | - |
| HLA-A*02:02 | GFKLKDCVMYA | - | 239 | - |
| HLA-A*02:02 | TSLSGFKLKDCVMYA | TSLKLKDCVMYA | - | 244 |
| HLA-A*03:01 | TSLSGFKLK | TSLKLK | 244 | - |
| HLA-B*15:03 | MVDTSLSGF | MVDTSL | 276 | - |
| HLA-A*68:01 | TSLSGFKLK | TSLKLK | 286 | - |
| HLA-A*03:02 | TSLSGFKLK | TSLKLK | 289 | - |
| HLA-A*03:01 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 309 |
| HLA-A*68:02 | DTSLSGFKL | DTSLKL | 313 | - |
| HLA-A*02:11 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 346 |
| HLA-B*15:01 | DMVDTSLSGF | DMVDTSL | 362 | - |
| HLA-A*23:01 | TWLDMVDTSLSGF | - | 387 | - |
| HLA-A*02:11 | GFKLKDCVMYA | - | 394 | - |
| HLA-A*03:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 415 |
| HLA-A*26:01 | DMVDTSLSGF | DMVDTSL | 417 | - |
| HLA-B*08:01 | MVDTSLSGFKLKDCVM | MVDTSLKLKDCVM | - | 426 |
| HLA-A*02:02 | WLDMVDTSLS | - | 441 | - |
| HLA-A*02:11 | FKLKDCVMYASAVV | - | 481 | - |
| HLA-A*11:01 | DTSLSGFKLK | DTSLKLK | 488 | - |
| HLA-B*35:01 | MVDTSLSGF | MVDTSL | 490 | - |
| HLA-A*31:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 493 |
| HLA-B*15:03 | LSGFKLKDCVMY | LKLKDCVMY | 3466 | 22 |
| HLA-A*02:11 | LDMVDTSLSGFKL | LDMVDTSLKL | 16396 | 166 |
| HLA-A*02:02 | LDMVDTSLSGFKL | LDMVDTSLKL | 9971 | 106 |
| HLA-A*02:11 | SLSGFKLKDCV | SLKLKDCV | 122 | 9934 |
| HLA-A*02:01 | LDMVDTSLSGFKL | LDMVDTSLKL | 20579 | 311 |
| HLA-B*08:01 | SLSGFKLKDCVM | SLKLKDCVM | 929 | 36 |
| HLA-A*02:02 | SLSGFKLKDCV | SLKLKDCV | 408 | 10216 |
| HLA-C*05:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 8375 | 417 |
| HLA-B*15:01 | SLSGFKLKDCVMY | SLKLKDCVMY | 2952 | 158 |
| HLA-B*08:01 | TSLSGFKLKDCVM | TSLKLKDCVM | 4628 | 289 |
| HLA-B*15:01 | SLSGFKLKDCVM | SLKLKDCVM | 2654 | 197 |
| HLA-A*68:01 | DMVDTSLSGFK | DMVDTSLK | 366 | 4919 |
| HLA-A*02:11 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 1342 | 108 |
| HLA-C*08:02 | MVDTSLSGFKL | MVDTSLKL | 2134 | 174 |
| HLA-B*15:03 | SLSGFKLKDCVM | SLKLKDCVM | 3634 | 297 |
| HLA-A*02:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 2214 | 195 |
| HLA-A*03:02 | WLDMVDTSLSGFK | WLDMVDTSLK | 1756 | 156 |
| HLA-A*02:02 | LSGFKLKDCVMYA | LKLKDCVMYA | 305 | 28 |
| HLA-A*02:01 | LSGFKLKDCVMYA | LKLKDCVMYA | 1788 | 165 |
| HLA-A*02:11 | LSGFKLKDCVMYA | LKLKDCVMYA | 578 | 54 |
| HLA-B*15:03 | SLSGFKLKDCVMY | SLKLKDCVMY | 3626 | 349 |
| HLA-A*03:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 2900 | 287 |
| HLA-C*05:01 | MVDTSLSGFKL | MVDTSLKL | 1125 | 124 |
| HLA-A*11:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 4020 | 492 |
| HLA-A*02:02 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 788 | 116 |
| HLA-A*68:01 | DMVDTSLSGFKLK | DMVDTSLKLK | 1679 | 251 |
| HLA-A*02:01 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 203 | 853 |
| HLA-A*02:11 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 64 | 223 |
| HLA-A*02:02 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 55 | 171 |
| HLA-A*29:02 | SLSGFKLKDCVMY | SLKLKDCVMY | 404 | 1192 |
| HLA-A*11:01 | MVDTSLSGFKLK | MVDTSLKLK | 143 | 50 |
| HLA-A*03:02 | MVDTSLSGFKLK | MVDTSLKLK | 312 | 127 |
| HLA-A*68:01 | MVDTSLSGFKLK | MVDTSLKLK | 602 | 246 |
| HLA-A*03:01 | MVDTSLSGFKLK | MVDTSLKLK | 441 | 190 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |
NSP13 protein
E341D
Reference ALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPET
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Mutated ALCEKALKYLPIDKCSRIIPARARVDCFDKFKVNSTLEQYVFCTVNALPET
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*42:01 | IPARARVEC | IPARARVDC | 60 | 315 |
| HLA-B*07:02 | IPARARVEC | IPARARVDC | 98 | 491 |
| HLA-A*02:11 | RVECFDKFKV | RVDCFDKFKV | 1174 | 238 |
| HLA-A*30:01 | RVECFDKFK | RVDCFDKFK | 337 | 1022 |
| HLA-A*02:06 | RVECFDKFKV | RVDCFDKFKV | 1311 | 435 |
| HLA-B*07:02 | IPARARVECF | IPARARVDCF | 45 | 117 |
| HLA-A*11:01 | ARVECFDKFK | ARVDCFDKFK | 876 | 354 |
| HLA-B*42:01 | IPARARVECF | IPARARVDCF | 45 | 96 |
| HLA-A*11:01 | RVECFDKFK | RVDCFDKFK | 195 | 92 |