Summary
This strain contains 20 protein-level mutations:
- 10 mutations in Spike protein: LGV 141-143 deletion, LA 242-243 deletion, Y265C, E484K, N501Y, D614G, P681H, E1092K, H1101Y, V1176F
- 1 mutation in N protein: 203-204 RG->KR
- 1 mutation in NS9c protein: G50N
- 2 mutations in NSP3 protein: D736G, S1807F
- 2 mutations in NSP4 protein: D217N, L438P
- 1 mutation in NSP7 protein: L71F
- 1 mutation in NSP12 protein: P323L
- 2 mutations in NSP13 protein: L280F, A368V
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
LGV 141-143 deletion
Reference SLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTF
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Mutated SLLIVNNATNVVIKVCEFQFCNDPF‑‑‑YYHKNNKSWMESEFRVYSSANNCTF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:06 | FQFCNDPFL | - | 4 | - |
| HLA-A*02:11 | FQFCNDPFL | - | 6 | - |
| HLA-A*02:06 | FQFCNDPFLGV | - | 7 | - |
| HLA-A*02:02 | FQFCNDPFL | - | 9 | - |
| HLA-A*02:01 | FQFCNDPFL | - | 10 | - |
| HLA-B*15:03 | FQFCNDPFL | - | 16 | - |
| HLA-A*02:11 | FQFCNDPFLGV | - | 17 | - |
| HLA-A*03:01 | GVYYHKNNK | YYHKNNK | 27 | - |
| HLA-A*02:01 | FQFCNDPFLGV | - | 39 | - |
| HLA-A*02:02 | FQFCNDPFLGV | - | 45 | - |
| HLA-A*11:01 | GVYYHKNNK | YYHKNNK | 51 | - |
| HLA-A*30:01 | GVYYHKNNK | YYHKNNK | 51 | - |
| HLA-A*02:06 | EFQFCNDPFLGV | - | 87 | - |
| HLA-A*02:11 | FCNDPFLGV | FCNDPF | 87 | - |
| HLA-A*02:06 | FCNDPFLGV | FCNDPF | 116 | - |
| HLA-A*03:02 | GVYYHKNNK | YYHKNNK | 119 | - |
| HLA-C*03:03 | FQFCNDPFL | - | 120 | - |
| HLA-C*03:04 | FQFCNDPFL | - | 120 | - |
| HLA-A*03:01 | LGVYYHKNNK | YYHKNNK | 131 | - |
| HLA-A*30:02 | KVCEFQFCNDPFLGVY | KVCEFQFCNDPFY | - | 164 |
| HLA-C*03:02 | FCNDPFLGVY | FCNDPFY | 164 | - |
| HLA-A*29:02 | CEFQFCNDPFLGVYY | CEFQFCNDPFYY | - | 181 |
| HLA-A*29:02 | VCEFQFCNDPFLGVYY | VCEFQFCNDPFYY | - | 182 |
| HLA-C*03:02 | FQFCNDPFL | - | 183 | - |
| HLA-A*01:01 | CNDPFLGVYY | CNDPFYY | 202 | - |
| HLA-A*02:06 | FQFCNDPFLG | - | 207 | - |
| HLA-B*38:01 | FQFCNDPFL | - | 221 | - |
| HLA-B*15:03 | KVCEFQFCNDPFLGVY | KVCEFQFCNDPFY | - | 222 |
| HLA-A*02:06 | EFQFCNDPFL | - | 227 | - |
| HLA-C*17:01 | FQFCNDPFL | - | 227 | - |
| HLA-A*23:01 | VYYHKNNKSW | - | 230 | - |
| HLA-A*01:01 | CNDPFLGVY | CNDPFY | 232 | - |
| HLA-A*29:02 | KVCEFQFCNDPFLGVY | KVCEFQFCNDPFY | - | 256 |
| HLA-B*44:03 | CEFQFCNDPFLGVYY | CEFQFCNDPFYY | - | 272 |
| HLA-A*33:03 | DPFLGVYYHK | DPFYYHK | 280 | - |
| HLA-A*68:02 | FCNDPFLGV | FCNDPF | 281 | - |
| HLA-B*15:03 | VCEFQFCNDPFLGVY | VCEFQFCNDPFY | - | 287 |
| HLA-A*30:02 | CNDPFLGVY | CNDPFY | 310 | - |
| HLA-C*12:02 | FQFCNDPFL | - | 315 | - |
| HLA-A*01:01 | FCNDPFLGVY | FCNDPFY | 317 | - |
| HLA-B*15:01 | FQFCNDPFL | - | 321 | - |
| HLA-A*24:02 | VYYHKNNKSW | - | 326 | - |
| HLA-C*14:02 | VYYHKNNKSWM | - | 327 | - |
| HLA-A*02:11 | EFQFCNDPFLGV | - | 334 | - |
| HLA-A*02:02 | EFQFCNDPFL | - | 341 | - |
| HLA-C*12:03 | FQFCNDPFL | - | 350 | - |
| HLA-A*68:01 | GVYYHKNNK | YYHKNNK | 363 | - |
| HLA-A*02:02 | FCNDPFLGV | FCNDPF | 365 | - |
| HLA-A*02:11 | KVCEFQFCNDPFL | - | 366 | - |
| HLA-A*11:01 | FQFCNDPFLGVYYHK | FQFCNDPFYYHK | - | 369 |
| HLA-A*31:01 | GVYYHKNNK | YYHKNNK | 375 | - |
| HLA-A*29:02 | KVCEFQFCNDPFLGVYY | KVCEFQFCNDPFYY | - | 376 |
| HLA-A*02:02 | FQFCNDPFLG | - | 392 | - |
| HLA-A*68:01 | DPFLGVYYHK | DPFYYHK | 398 | - |
| HLA-A*30:02 | FCNDPFLGVY | FCNDPFY | 408 | - |
| HLA-A*29:02 | CNDPFLGVYY | CNDPFYY | 439 | - |
| HLA-A*30:02 | KVCEFQFCNDPFLGVYY | KVCEFQFCNDPFYY | - | 439 |
| HLA-A*31:01 | FQFCNDPFLGVYYHK | FQFCNDPFYYHK | - | 450 |
| HLA-C*16:01 | FQFCNDPFL | - | 473 | - |
| HLA-A*02:02 | EFQFCNDPFLGV | - | 477 | - |
| HLA-A*02:06 | KVCEFQFCNDPFL | - | 477 | - |
| HLA-A*68:01 | LGVYYHKNNK | YYHKNNK | 494 | - |
| HLA-C*14:02 | FLGVYYHKNNKSWM | FYYHKNNKSWM | 11817 | 69 |
| HLA-A*23:01 | FLGVYYHKNNKSW | FYYHKNNKSW | 7046 | 159 |
| HLA-A*03:01 | FLGVYYHKNNK | FYYHKNNK | 455 | 18399 |
| HLA-A*29:02 | FQFCNDPFLGVYY | FQFCNDPFYY | 184 | 5 |
| HLA-A*24:02 | FLGVYYHKNNKSW | FYYHKNNKSW | 8091 | 252 |
| HLA-C*14:02 | FLGVYYHKNNKSW | FYYHKNNKSW | 7891 | 285 |
| HLA-A*30:02 | FQFCNDPFLGVYY | FQFCNDPFYY | 1123 | 45 |
| HLA-A*29:02 | EFQFCNDPFLGVYY | EFQFCNDPFYY | 1507 | 68 |
| HLA-C*02:02 | FQFCNDPFLGVYY | FQFCNDPFYY | 4047 | 227 |
| HLA-C*02:10 | FQFCNDPFLGVYY | FQFCNDPFYY | 4047 | 227 |
| HLA-B*35:01 | FQFCNDPFLGVY | FQFCNDPFY | 1932 | 111 |
| HLA-A*29:02 | FQFCNDPFLGVYYH | FQFCNDPFYYH | 5473 | 318 |
| HLA-C*03:02 | FQFCNDPFLGVY | FQFCNDPFY | 736 | 47 |
| HLA-A*29:02 | EFQFCNDPFLGVY | EFQFCNDPFY | 1629 | 107 |
| HLA-C*03:02 | FQFCNDPFLGVYY | FQFCNDPFYY | 2016 | 144 |
| HLA-A*29:02 | FQFCNDPFLGVY | FQFCNDPFY | 388 | 28 |
| HLA-B*35:01 | FQFCNDPFLGVYY | FQFCNDPFYY | 3992 | 297 |
| HLA-C*16:01 | FQFCNDPFLGVY | FQFCNDPFY | 5396 | 421 |
| HLA-C*12:03 | FQFCNDPFLGVYY | FQFCNDPFYY | 4770 | 486 |
| HLA-C*12:02 | FQFCNDPFLGVYY | FQFCNDPFYY | 2524 | 259 |
| HLA-A*29:02 | QFCNDPFLGVY | QFCNDPFY | 192 | 1821 |
| HLA-A*29:02 | QFCNDPFLGVYY | QFCNDPFYY | 113 | 13 |
| HLA-B*15:03 | EFQFCNDPFLGVYY | EFQFCNDPFYY | 1912 | 249 |
| HLA-C*02:02 | FQFCNDPFLGVY | FQFCNDPFY | 2256 | 303 |
| HLA-C*02:10 | FQFCNDPFLGVY | FQFCNDPFY | 2256 | 303 |
| HLA-C*12:03 | FQFCNDPFLGVY | FQFCNDPFY | 2521 | 346 |
| HLA-B*15:03 | FQFCNDPFLGVYYH | FQFCNDPFYYH | 1280 | 202 |
| HLA-A*68:01 | FCNDPFLGVYYHK | FCNDPFYYHK | 3027 | 499 |
| HLA-B*44:03 | CEFQFCNDPFLGVY | CEFQFCNDPFY | 2838 | 469 |
| HLA-C*12:02 | FQFCNDPFLGVY | FQFCNDPFY | 1193 | 202 |
| HLA-B*15:03 | FQFCNDPFLGVYY | FQFCNDPFYY | 47 | 8 |
| HLA-A*30:02 | QFCNDPFLGVYY | QFCNDPFYY | 1591 | 292 |
| HLA-B*15:01 | FQFCNDPFLGVYY | FQFCNDPFYY | 129 | 25 |
| HLA-A*02:11 | FQFCNDPFLGVY | FQFCNDPFY | 488 | 1987 |
| HLA-B*15:03 | CEFQFCNDPFLGVY | CEFQFCNDPFY | 967 | 301 |
| HLA-A*30:02 | FQFCNDPFLGVY | FQFCNDPFY | 336 | 111 |
| HLA-A*02:06 | FQFCNDPFLGVYY | FQFCNDPFYY | 1024 | 367 |
| HLA-B*15:03 | FQFCNDPFLGVY | FQFCNDPFY | 11 | 4 |
| HLA-A*02:06 | FQFCNDPFLGVY | FQFCNDPFY | 135 | 290 |
| HLA-B*15:01 | EFQFCNDPFLGVY | EFQFCNDPFY | 953 | 470 |
LA 242-243 deletion
Reference PQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVG
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Mutated PQGFSALEPLVDLPIGINITRFQTL‑‑LHRSYLTPGDSSSGWTAGAAACYVG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | ITRFQTLLA | ITRFQTL | 12 | - |
| HLA-A*02:02 | LLALHRSYL | LLHRSYL | 13 | - |
| HLA-A*02:11 | LLALHRSYL | LLHRSYL | 21 | - |
| HLA-B*15:03 | TLLALHRSY | TLLHRSY | 28 | - |
| HLA-B*08:01 | LLALHRSYL | LLHRSYL | 30 | - |
| HLA-B*27:05 | TRFQTLLAL | TRFQTLL | 35 | - |
| HLA-B*27:05 | ITRFQTLLAL | - | 60 | - |
| HLA-C*06:02 | TRFQTLLAL | TRFQTLL | 65 | - |
| HLA-A*29:02 | TLLALHRSY | TLLHRSY | 67 | - |
| HLA-C*07:01 | TRFQTLLAL | TRFQTLL | 71 | - |
| HLA-B*15:03 | TRFQTLLAL | TRFQTLL | 75 | - |
| HLA-C*07:02 | TRFQTLLAL | TRFQTLL | 88 | - |
| HLA-C*14:02 | RFQTLLAL | RFQTLL | 91 | - |
| HLA-B*27:05 | GINITRFQTLLAL | - | 99 | - |
| HLA-C*16:01 | LALHRSYL | LHRSYL | 101 | - |
| HLA-B*15:01 | TLLALHRSY | TLLHRSY | 106 | - |
| HLA-B*14:02 | TRFQTLLAL | TRFQTLL | 120 | - |
| HLA-A*30:01 | ITRFQTLLAL | - | 126 | - |
| HLA-B*42:01 | LLALHRSYL | LLHRSYL | 170 | - |
| HLA-A*30:02 | TLLALHRSY | TLLHRSY | 172 | - |
| HLA-C*14:02 | TRFQTLLAL | TRFQTLL | 203 | - |
| HLA-B*27:05 | INITRFQTLLAL | - | 213 | - |
| HLA-A*30:01 | NITRFQTLLA | - | 214 | - |
| HLA-B*08:01 | LALHRSYL | LHRSYL | 242 | - |
| HLA-A*31:01 | RFQTLLALH | RFQTLLH | 258 | - |
| HLA-B*27:05 | NITRFQTLLAL | - | 258 | - |
| HLA-B*27:05 | GINITRFQTLLALHR | GINITRFQTLLHR | - | 275 |
| HLA-B*15:03 | LLALHRSY | LLHRSY | 313 | - |
| HLA-A*30:02 | RFQTLLALH | RFQTLLH | 338 | - |
| HLA-A*02:01 | LLALHRSYL | LLHRSYL | 353 | - |
| HLA-A*30:01 | INITRFQTLLA | - | 356 | - |
| HLA-C*03:02 | TLLALHRSY | TLLHRSY | 362 | - |
| HLA-B*15:01 | LLALHRSY | LLHRSY | 395 | - |
| HLA-C*14:02 | LLALHRSYL | LLHRSYL | 407 | - |
| HLA-C*16:01 | LLALHRSYL | LLHRSYL | 420 | - |
| HLA-A*02:06 | ITRFQTLLAL | - | 431 | - |
| HLA-B*15:03 | ITRFQTLLAL | - | 451 | - |
| HLA-B*15:03 | LLALHRSYL | LLHRSYL | 458 | - |
| HLA-A*02:02 | TLLALHRSYL | TLLHRSYL | 59 | 4272 |
| HLA-C*03:02 | FQTLLALHRSY | FQTLLHRSY | 2052 | 32 |
| HLA-A*02:11 | TLLALHRSYL | TLLHRSYL | 33 | 2034 |
| HLA-C*12:03 | FQTLLALHRSY | FQTLLHRSY | 6248 | 108 |
| HLA-C*16:01 | FQTLLALHRSY | FQTLLHRSY | 6440 | 113 |
| HLA-A*02:02 | LLALHRSYLT | LLHRSYLT | 306 | 12739 |
| HLA-A*02:01 | TLLALHRSYL | TLLHRSYL | 174 | 6508 |
| HLA-C*16:01 | QTLLALHRSYL | QTLLHRSYL | 8730 | 259 |
| HLA-B*27:05 | INITRFQTLLALHR | INITRFQTLLHR | 11794 | 422 |
| HLA-C*12:02 | FQTLLALHRSY | FQTLLHRSY | 3734 | 139 |
| HLA-B*27:05 | TRFQTLLALH | TRFQTLLH | 147 | 3386 |
| HLA-A*11:01 | ITRFQTLLALHR | ITRFQTLLHR | 5196 | 255 |
| HLA-A*30:01 | QTLLALHRSYL | QTLLHRSYL | 4016 | 201 |
| HLA-A*31:01 | TRFQTLLALHR | TRFQTLLHR | 145 | 2825 |
| HLA-A*02:11 | FQTLLALHRSYLT | FQTLLHRSYLT | 5635 | 296 |
| HLA-A*31:01 | RFQTLLALHRS | RFQTLLHRS | 348 | 6089 |
| HLA-C*14:02 | FQTLLALHRSY | FQTLLHRSY | 6244 | 357 |
| HLA-B*15:03 | RFQTLLALHRSY | RFQTLLHRSY | 340 | 20 |
| HLA-C*02:02 | FQTLLALHRSY | FQTLLHRSY | 6171 | 380 |
| HLA-C*02:10 | FQTLLALHRSY | FQTLLHRSY | 6171 | 380 |
| HLA-A*68:01 | ITRFQTLLALHR | ITRFQTLLHR | 1475 | 107 |
| HLA-B*15:01 | QTLLALHRSY | QTLLHRSY | 363 | 4986 |
| HLA-A*30:01 | ITRFQTLLALH | ITRFQTLLH | 3187 | 238 |
| HLA-A*02:11 | TLLALHRSYLT | TLLHRSYLT | 944 | 85 |
| HLA-B*27:05 | ITRFQTLLALHR | ITRFQTLLHR | 1674 | 151 |
| HLA-A*33:03 | RFQTLLALHR | RFQTLLHR | 231 | 2560 |
| HLA-A*30:02 | QTLLALHRSY | QTLLHRSY | 214 | 2338 |
| HLA-B*15:03 | ITRFQTLLALHRSY | ITRFQTLLHRSY | 2610 | 265 |
| HLA-A*31:01 | RFQTLLALHR | RFQTLLHR | 12 | 109 |
| HLA-B*18:01 | FQTLLALHRSY | FQTLLHRSY | 3454 | 436 |
| HLA-B*15:01 | RFQTLLALHRSY | RFQTLLHRSY | 650 | 84 |
| HLA-A*30:01 | RFQTLLALHRSYL | RFQTLLHRSYL | 2049 | 278 |
| HLA-B*15:03 | FQTLLALHRSY | FQTLLHRSY | 29 | 4 |
| HLA-C*14:02 | RFQTLLALHRSY | RFQTLLHRSY | 1204 | 203 |
| HLA-B*15:03 | FQTLLALHRSYL | FQTLLHRSYL | 592 | 105 |
| HLA-B*15:01 | FQTLLALHRSYL | FQTLLHRSYL | 1826 | 412 |
| HLA-A*33:03 | ITRFQTLLALHR | ITRFQTLLHR | 656 | 156 |
| HLA-B*15:01 | FQTLLALHRSY | FQTLLHRSY | 126 | 32 |
| HLA-B*27:05 | TRFQTLLALHRSYL | TRFQTLLHRSYL | 500 | 145 |
| HLA-A*68:01 | NITRFQTLLALHR | NITRFQTLLHR | 652 | 198 |
| HLA-B*15:03 | TRFQTLLALHRSY | TRFQTLLHRSY | 396 | 124 |
| HLA-A*30:02 | FQTLLALHRSY | FQTLLHRSY | 1459 | 488 |
| HLA-A*31:01 | ITRFQTLLALHR | ITRFQTLLHR | 134 | 45 |
| HLA-A*29:02 | RFQTLLALHRSY | RFQTLLHRSY | 489 | 172 |
| HLA-A*30:02 | RFQTLLALHRSY | RFQTLLHRSY | 140 | 51 |
| HLA-A*02:11 | FQTLLALHRSYL | FQTLLHRSYL | 249 | 628 |
| HLA-A*02:06 | FQTLLALHRSYL | FQTLLHRSYL | 579 | 238 |
| HLA-B*27:05 | TRFQTLLALHR | TRFQTLLHR | 191 | 82 |
| HLA-A*30:02 | ITRFQTLLALHRSY | ITRFQTLLHRSY | 715 | 309 |
| HLA-B*27:05 | TRFQTLLALHRS | TRFQTLLHRS | 1078 | 482 |
| HLA-A*33:03 | NITRFQTLLALHR | NITRFQTLLHR | 548 | 261 |
Y265C
Reference TLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVD
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Mutated TL‑‑LHRSYLTPGDSSSGWTAGAAACYVGYLQPRTFLLKYNENGTITDAVD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:02 | SGWTAGAAAY | SGWTAGAAAC | 100 | 19672 |
| HLA-A*30:02 | SSSGWTAGAAAY | SSSGWTAGAAAC | 108 | 18810 |
| HLA-A*30:02 | SSGWTAGAAAY | SSGWTAGAAAC | 152 | 20737 |
| HLA-B*15:03 | SGWTAGAAAY | SGWTAGAAAC | 127 | 15161 |
| HLA-A*29:02 | SGWTAGAAAY | SGWTAGAAAC | 368 | 32767 |
| HLA-A*29:02 | GWTAGAAAY | GWTAGAAAC | 387 | 32767 |
| HLA-A*01:01 | SSSGWTAGAAAY | SSSGWTAGAAAC | 469 | 29119 |
| HLA-B*15:01 | SGWTAGAAAY | SGWTAGAAAC | 417 | 25851 |
| HLA-C*03:02 | WTAGAAAY | WTAGAAAC | 368 | 19293 |
| HLA-C*14:02 | GWTAGAAAY | GWTAGAAAC | 323 | 13881 |
| HLA-C*14:02 | AYYVGYLQPRTF | ACYVGYLQPRTF | 174 | 5593 |
| HLA-A*23:01 | AYYVGYLQPRTF | ACYVGYLQPRTF | 69 | 1870 |
| HLA-A*23:01 | AYYVGYLQPRTFLL | ACYVGYLQPRTFLL | 373 | 8243 |
| HLA-A*24:02 | AYYVGYLQPRTF | ACYVGYLQPRTF | 215 | 3791 |
| HLA-A*02:11 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 52 | 872 |
| HLA-C*14:02 | YYVGYLQPRTF | CYVGYLQPRTF | 26 | 382 |
| HLA-C*07:02 | YYVGYLQPRTF | CYVGYLQPRTF | 205 | 2945 |
| HLA-C*14:02 | YYVGYLQPRTFL | CYVGYLQPRTFL | 50 | 657 |
| HLA-C*14:02 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 121 | 1546 |
| HLA-A*02:01 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 257 | 3227 |
| HLA-A*02:02 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 88 | 969 |
| HLA-A*26:01 | DSSSGWTAGAAAYY | DSSSGWTAGAAACY | 344 | 3059 |
| HLA-A*68:01 | GWTAGAAAYY | GWTAGAAACY | 500 | 3832 |
| HLA-A*68:01 | WTAGAAAYY | WTAGAAACY | 22 | 167 |
| HLA-A*29:02 | SSGWTAGAAAYY | SSGWTAGAAACY | 301 | 2058 |
| HLA-A*26:01 | SSGWTAGAAAYY | SSGWTAGAAACY | 438 | 2795 |
| HLA-A*26:01 | GWTAGAAAYY | GWTAGAAACY | 318 | 1985 |
| HLA-A*29:02 | SGWTAGAAAYY | SGWTAGAAACY | 239 | 1462 |
| HLA-A*23:01 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 68 | 386 |
| HLA-A*29:02 | GWTAGAAAYY | GWTAGAAACY | 81 | 453 |
| HLA-A*68:02 | TAGAAAYYV | TAGAAACYV | 30 | 166 |
| HLA-C*02:02 | WTAGAAAYY | WTAGAAACY | 66 | 361 |
| HLA-C*02:10 | WTAGAAAYY | WTAGAAACY | 66 | 361 |
| HLA-A*29:02 | SSSGWTAGAAAYY | SSSGWTAGAAACY | 337 | 1807 |
| HLA-A*24:02 | YYVGYLQPRTFLL | CYVGYLQPRTFLL | 89 | 457 |
| HLA-A*02:11 | TAGAAAYYV | TAGAAACYV | 107 | 528 |
| HLA-A*29:02 | WTAGAAAYY | WTAGAAACY | 8 | 39 |
| HLA-C*14:02 | YYVGYLQPR | CYVGYLQPR | 492 | 2217 |
| HLA-A*24:02 | YYVGYLQPRTF | CYVGYLQPRTF | 30 | 130 |
| HLA-A*23:01 | YYVGYLQPRTFL | CYVGYLQPRTFL | 238 | 989 |
| HLA-A*30:02 | GWTAGAAAYY | GWTAGAAACY | 46 | 191 |
| HLA-C*12:03 | WTAGAAAYY | WTAGAAACY | 52 | 203 |
| HLA-A*31:01 | AYYVGYLQPR | ACYVGYLQPR | 19 | 74 |
| HLA-A*26:01 | WTAGAAAYY | WTAGAAACY | 9 | 35 |
| HLA-C*12:03 | GAAAYYVGY | GAAACYVGY | 258 | 995 |
| HLA-A*02:06 | TAGAAAYYV | TAGAAACYV | 186 | 714 |
| HLA-A*23:01 | YYVGYLQPRTF | CYVGYLQPRTF | 17 | 63 |
| HLA-A*24:02 | YYVGYLQPRTFL | CYVGYLQPRTFL | 474 | 1671 |
| HLA-A*68:01 | AAYYVGYLQPR | AACYVGYLQPR | 88 | 305 |
| HLA-A*30:02 | WTAGAAAYY | WTAGAAACY | 25 | 85 |
| HLA-A*25:01 | WTAGAAAYY | WTAGAAACY | 112 | 380 |
| HLA-A*29:02 | WTAGAAAYYVGY | WTAGAAACYVGY | 175 | 588 |
| HLA-A*30:02 | SGWTAGAAAYY | SGWTAGAAACY | 90 | 296 |
| HLA-C*12:02 | WTAGAAAYY | WTAGAAACY | 71 | 232 |
| HLA-C*14:02 | WTAGAAAYY | WTAGAAACY | 249 | 758 |
| HLA-A*30:02 | SSGWTAGAAAYY | SSGWTAGAAACY | 57 | 166 |
| HLA-A*02:02 | TAGAAAYYV | TAGAAACYV | 340 | 979 |
| HLA-A*30:02 | SSSGWTAGAAAYY | SSSGWTAGAAACY | 80 | 226 |
| HLA-A*01:01 | GWTAGAAAYY | GWTAGAAACY | 258 | 695 |
| HLA-B*15:03 | GAAAYYVGY | GAAACYVGY | 46 | 122 |
| HLA-C*16:01 | GAAAYYVGY | GAAACYVGY | 166 | 430 |
| HLA-A*01:01 | SSGWTAGAAAYY | SSGWTAGAAACY | 330 | 853 |
| HLA-A*01:01 | SSSGWTAGAAAYY | SSSGWTAGAAACY | 231 | 593 |
| HLA-C*03:02 | WTAGAAAYY | WTAGAAACY | 16 | 41 |
| HLA-C*03:02 | GAAAYYVGY | GAAACYVGY | 99 | 247 |
| HLA-B*15:03 | WTAGAAAYY | WTAGAAACY | 48 | 116 |
| HLA-C*16:01 | WTAGAAAYY | WTAGAAACY | 52 | 125 |
| HLA-A*68:01 | WTAGAAAYYVGY | WTAGAAACYVGY | 479 | 1146 |
| HLA-C*12:02 | GAAAYYVGY | GAAACYVGY | 356 | 837 |
| HLA-C*03:03 | AAAYYVGYL | AAACYVGYL | 49 | 106 |
| HLA-C*03:04 | AAAYYVGYL | AAACYVGYL | 49 | 106 |
| HLA-A*01:01 | WTAGAAAYYVGY | WTAGAAACYVGY | 174 | 374 |
| HLA-C*12:03 | AAAYYVGYL | AAACYVGYL | 29 | 62 |
| HLA-C*16:01 | AAAYYVGYL | AAACYVGYL | 30 | 63 |
| HLA-C*03:02 | AAAYYVGYL | AAACYVGYL | 117 | 241 |
| HLA-A*01:01 | WTAGAAAYY | WTAGAAACY | 18 | 37 |
| HLA-A*30:02 | WTAGAAAYYVGY | WTAGAAACYVGY | 313 | 634 |
| HLA-A*02:11 | WTAGAAAYYV | WTAGAAACYV | 79 | 160 |
| HLA-B*35:01 | GAAAYYVGY | GAAACYVGY | 487 | 985 |
E484K
Reference SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:02 | VEGFNCYFPL | VKGFNCYFPL | 31 | 7011 |
| HLA-B*40:01 | VEGFNCYFPL | VKGFNCYFPL | 76 | 15210 |
| HLA-A*11:01 | GSTPCNGVE | GSTPCNGVK | 32767 | 444 |
| HLA-A*68:02 | EGFNCYFPL | KGFNCYFPL | 117 | 3877 |
| HLA-A*30:01 | EGFNCYFPL | KGFNCYFPL | 5413 | 164 |
| HLA-A*32:01 | EGFNCYFPL | KGFNCYFPL | 6548 | 210 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQSY | 1806 | 72 |
| HLA-B*15:01 | GVEGFNCYF | GVKGFNCYF | 3125 | 132 |
| HLA-A*31:01 | EGFNCYFPL | KGFNCYFPL | 4227 | 226 |
| HLA-B*15:03 | EGFNCYFPL | KGFNCYFPL | 2980 | 212 |
| HLA-A*02:11 | EGFNCYFPL | KGFNCYFPL | 2508 | 259 |
| HLA-B*15:03 | GVEGFNCYF | GVKGFNCYF | 1657 | 199 |
| HLA-A*02:06 | EGFNCYFPL | KGFNCYFPL | 1266 | 169 |
| HLA-B*35:01 | TPCNGVEGF | TPCNGVKGF | 319 | 1754 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQSY | 1081 | 208 |
| HLA-B*35:01 | NGVEGFNCY | NGVKGFNCY | 346 | 1439 |
| HLA-C*16:01 | EGFNCYFPL | KGFNCYFPL | 1861 | 498 |
| HLA-B*15:03 | VEGFNCYFPL | VKGFNCYFPL | 943 | 280 |
N501Y
Reference GSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*29:02 | QSYGFQPTN | QSYGFQPTY | 26152 | 24 |
| HLA-A*29:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 19514 | 25 |
| HLA-B*15:01 | LQSYGFQPTN | LQSYGFQPTY | 6135 | 8 |
| HLA-B*35:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8715 | 15 |
| HLA-C*03:02 | QSYGFQPTN | QSYGFQPTY | 12903 | 23 |
| HLA-B*15:03 | LQSYGFQPTN | LQSYGFQPTY | 2045 | 4 |
| HLA-B*15:03 | QSYGFQPTN | QSYGFQPTY | 6543 | 13 |
| HLA-C*16:01 | QSYGFQPTN | QSYGFQPTY | 10868 | 27 |
| HLA-B*35:01 | QSYGFQPTN | QSYGFQPTY | 24615 | 71 |
| HLA-C*12:03 | QSYGFQPTN | QSYGFQPTY | 12696 | 38 |
| HLA-B*15:03 | PLQSYGFQPTN | PLQSYGFQPTY | 22802 | 80 |
| HLA-C*14:02 | TNGVGYQPY | TYGVGYQPY | 5468 | 21 |
| HLA-A*29:02 | LQSYGFQPTN | LQSYGFQPTY | 28458 | 133 |
| HLA-C*03:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 23840 | 113 |
| HLA-C*03:02 | LQSYGFQPTN | LQSYGFQPTY | 23421 | 125 |
| HLA-A*30:02 | LQSYGFQPTN | LQSYGFQPTY | 7508 | 42 |
| HLA-A*30:02 | QSYGFQPTN | QSYGFQPTY | 5297 | 30 |
| HLA-B*15:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 20159 | 137 |
| HLA-C*03:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 27639 | 198 |
| HLA-A*29:02 | CYFPLQSYGFQPTN | CYFPLQSYGFQPTY | 27182 | 196 |
| HLA-C*14:02 | SYGFQPTN | SYGFQPTY | 20230 | 162 |
| HLA-A*29:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 32767 | 264 |
| HLA-B*15:01 | QSYGFQPTN | QSYGFQPTY | 21529 | 195 |
| HLA-C*14:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18230 | 169 |
| HLA-B*15:03 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 22168 | 206 |
| HLA-A*11:01 | QSYGFQPTN | QSYGFQPTY | 22068 | 241 |
| HLA-C*16:01 | LQSYGFQPTN | LQSYGFQPTY | 27149 | 326 |
| HLA-C*12:02 | QSYGFQPTN | QSYGFQPTY | 21029 | 267 |
| HLA-B*15:01 | PLQSYGFQPTN | PLQSYGFQPTY | 32767 | 449 |
| HLA-B*35:01 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 28375 | 395 |
| HLA-C*12:03 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27893 | 431 |
| HLA-C*16:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 27733 | 434 |
| HLA-C*02:02 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-C*02:10 | QSYGFQPTN | QSYGFQPTY | 26598 | 425 |
| HLA-A*30:02 | YFPLQSYGFQPTN | YFPLQSYGFQPTY | 18743 | 310 |
| HLA-B*15:03 | LQSYGFQPTNG | LQSYGFQPTYG | 6387 | 109 |
| HLA-B*53:01 | FPLQSYGFQPTN | FPLQSYGFQPTY | 8451 | 151 |
| HLA-C*12:03 | LQSYGFQPTN | LQSYGFQPTY | 26641 | 480 |
| HLA-A*32:01 | QSYGFQPTN | QSYGFQPTY | 16619 | 311 |
| HLA-A*30:02 | FPLQSYGFQPTN | FPLQSYGFQPTY | 21475 | 466 |
| HLA-C*03:02 | NGVGYQPY | YGVGYQPY | 10463 | 451 |
| HLA-B*57:01 | QSYGFQPTN | QSYGFQPTY | 4070 | 215 |
| HLA-B*58:01 | QSYGFQPTN | QSYGFQPTY | 1532 | 83 |
| HLA-A*02:11 | NGVGYQPYRV | YGVGYQPYRV | 5989 | 327 |
| HLA-B*57:01 | LQSYGFQPTN | LQSYGFQPTY | 3839 | 244 |
| HLA-B*58:01 | LQSYGFQPTN | LQSYGFQPTY | 1885 | 155 |
| HLA-B*15:03 | NGVGYQPY | YGVGYQPY | 4677 | 467 |
| HLA-A*31:01 | TNGVGYQPYR | TYGVGYQPYR | 811 | 84 |
| HLA-A*33:03 | TNGVGYQPYR | TYGVGYQPYR | 573 | 62 |
| HLA-A*29:02 | TNGVGYQPY | TYGVGYQPY | 2031 | 237 |
| HLA-A*29:02 | PTNGVGYQPY | PTYGVGYQPY | 1211 | 177 |
| HLA-A*02:11 | YGFQPTNGV | YGFQPTYGV | 307 | 49 |
| HLA-A*02:01 | YGFQPTNGV | YGFQPTYGV | 2160 | 459 |
| HLA-A*68:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1073 | 242 |
| HLA-A*02:11 | LQSYGFQPTNGV | LQSYGFQPTYGV | 1446 | 396 |
| HLA-A*31:01 | PTNGVGYQPYR | PTYGVGYQPYR | 1377 | 406 |
| HLA-A*02:06 | YGFQPTNGV | YGFQPTYGV | 174 | 52 |
| HLA-A*33:03 | PTNGVGYQPYR | PTYGVGYQPYR | 1345 | 407 |
| HLA-A*02:02 | YGFQPTNGV | YGFQPTYGV | 868 | 287 |
| HLA-A*02:06 | QSYGFQPTNGV | QSYGFQPTYGV | 1121 | 407 |
| HLA-A*68:02 | YGFQPTNGV | YGFQPTYGV | 73 | 28 |
| HLA-A*68:02 | QSYGFQPTNGV | QSYGFQPTYGV | 122 | 48 |
| HLA-B*15:01 | GFQPTNGVGY | GFQPTYGVGY | 774 | 311 |
| HLA-A*29:02 | YGFQPTNGVGY | YGFQPTYGVGY | 1158 | 486 |
| HLA-A*31:01 | NGVGYQPYR | YGVGYQPYR | 438 | 185 |
| HLA-A*02:06 | LQSYGFQPTNGV | LQSYGFQPTYGV | 249 | 111 |
| HLA-A*30:02 | PTNGVGYQPY | PTYGVGYQPY | 465 | 210 |
| HLA-B*15:03 | FQPTNGVGY | FQPTYGVGY | 63 | 29 |
| HLA-A*02:02 | LQSYGFQPTNGV | LQSYGFQPTYGV | 594 | 280 |
| HLA-B*15:03 | GFQPTNGVGY | GFQPTYGVGY | 769 | 363 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
P681H
Reference VNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVA
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VNNSYECDIPIGAGICASYQTQTNSHRRARSVASQSIIAYTMSLGAENSVA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*07:02 | SPRRARSVA | SHRRARSVA | 4 | 4721 |
| HLA-B*42:01 | SPRRARSVA | SHRRARSVA | 8 | 6169 |
| HLA-B*07:02 | SPRRARSVASQSI | SHRRARSVASQSI | 17 | 11433 |
| HLA-B*07:02 | SPRRARSV | SHRRARSV | 24 | 12728 |
| HLA-B*07:02 | SPRRARSVAS | SHRRARSVAS | 37 | 14758 |
| HLA-B*07:02 | SPRRARSVASQSII | SHRRARSVASQSII | 55 | 17736 |
| HLA-B*42:01 | SPRRARSV | SHRRARSV | 38 | 11622 |
| HLA-B*42:01 | SPRRARSVASQSI | SHRRARSVASQSI | 48 | 14418 |
| HLA-B*07:02 | TNSPRRARSVA | TNSHRRARSVA | 125 | 20078 |
| HLA-B*42:01 | SPRRARSVAS | SHRRARSVAS | 158 | 17461 |
| HLA-B*07:02 | NSPRRARSVA | NSHRRARSVA | 69 | 7270 |
| HLA-B*42:01 | SPRRARSVASQSII | SHRRARSVASQSII | 321 | 21521 |
| HLA-B*07:02 | TQTNSPRRARSVA | TQTNSHRRARSVA | 270 | 17880 |
| HLA-B*07:02 | QTNSPRRARSVA | QTNSHRRARSVA | 249 | 13539 |
| HLA-B*42:01 | TNSPRRARSVA | TNSHRRARSVA | 437 | 21599 |
| HLA-B*42:01 | NSPRRARSVA | NSHRRARSVA | 217 | 8330 |
| HLA-C*14:02 | SYQTQTNSP | SYQTQTNSH | 3691 | 139 |
| HLA-B*08:01 | NSPRRARSV | NSHRRARSV | 881 | 69 |
| HLA-B*08:01 | SPRRARSVA | SHRRARSVA | 372 | 3831 |
| HLA-B*08:01 | SPRRARSV | SHRRARSV | 428 | 2448 |
| HLA-C*16:01 | NSPRRARSV | NSHRRARSV | 1247 | 314 |
| HLA-C*12:03 | NSPRRARSV | NSHRRARSV | 1110 | 365 |
| HLA-A*11:01 | ASYQTQTNSPR | ASYQTQTNSHR | 346 | 1041 |
| HLA-A*68:01 | CASYQTQTNSPR | CASYQTQTNSHR | 142 | 401 |
| HLA-A*68:01 | TQTNSPRRAR | TQTNSHRRAR | 254 | 710 |
| HLA-A*31:01 | YQTQTNSPR | YQTQTNSHR | 441 | 1214 |
| HLA-A*68:01 | YQTQTNSPRR | YQTQTNSHRR | 206 | 78 |
| HLA-A*33:03 | QTQTNSPRR | QTQTNSHRR | 445 | 176 |
| HLA-A*33:03 | YQTQTNSPRR | YQTQTNSHRR | 967 | 411 |
| HLA-A*68:01 | QTQTNSPRR | QTQTNSHRR | 119 | 51 |
| HLA-A*68:01 | QTNSPRRAR | QTNSHRRAR | 95 | 216 |
| HLA-A*31:01 | ASYQTQTNSPR | ASYQTQTNSHR | 139 | 311 |
E1092K
Reference YVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITT
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated YVPAQEKNFTTAPAICHDGKAHFPRKGVFVSNGTYWFVTQRNFYEPQIITT
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:02 | EGVFVSNGTH | KGVFVSNGTY | 10999 | 20 |
| HLA-B*15:01 | EGVFVSNGTH | KGVFVSNGTY | 16651 | 79 |
| HLA-B*15:03 | EGVFVSNGTH | KGVFVSNGTY | 17087 | 92 |
| HLA-B*44:02 | REGVFVSNGTHW | RKGVFVSNGTYW | 77 | 13384 |
| HLA-B*44:03 | REGVFVSNGTHW | RKGVFVSNGTYW | 137 | 15881 |
| HLA-B*15:03 | REGVFVSNGTH | RKGVFVSNGTY | 3026 | 27 |
| HLA-A*30:01 | HDGKAHFPRE | HDGKAHFPRK | 6388 | 92 |
| HLA-B*35:01 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 2599 | 42 |
| HLA-A*30:02 | REGVFVSNGTH | RKGVFVSNGTY | 3495 | 60 |
| HLA-B*15:01 | REGVFVSNGTH | RKGVFVSNGTY | 10166 | 217 |
| HLA-A*30:02 | PREGVFVSNGTH | PRKGVFVSNGTY | 10319 | 239 |
| HLA-B*15:01 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 12794 | 378 |
| HLA-A*30:02 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 13269 | 467 |
| HLA-A*30:02 | HFPREGVFVSNGTH | HFPRKGVFVSNGTY | 12489 | 498 |
| HLA-B*58:01 | EGVFVSNGTHW | KGVFVSNGTYW | 1949 | 79 |
| HLA-B*57:01 | EGVFVSNGTHW | KGVFVSNGTYW | 2892 | 221 |
| HLA-A*68:02 | EGVFVSNGTHWFV | KGVFVSNGTYWFV | 355 | 4131 |
| HLA-B*07:02 | FPREGVFV | FPRKGVFV | 749 | 262 |
| HLA-B*42:01 | FPREGVFV | FPRKGVFV | 163 | 63 |
| HLA-B*35:01 | FPREGVFVS | FPRKGVFVS | 119 | 302 |
| HLA-B*07:02 | FPREGVFVS | FPRKGVFVS | 424 | 176 |
H1101Y
Reference TTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNC
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TTAPAICHDGKAHFPRKGVFVSNGTYWFVTQRNFYEPQIITTDNTFVSGNC
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:02 | EGVFVSNGTH | KGVFVSNGTY | 10999 | 20 |
| HLA-B*15:01 | EGVFVSNGTH | KGVFVSNGTY | 16651 | 79 |
| HLA-B*15:03 | EGVFVSNGTH | KGVFVSNGTY | 17087 | 92 |
| HLA-B*44:02 | REGVFVSNGTHW | RKGVFVSNGTYW | 77 | 13384 |
| HLA-B*44:03 | REGVFVSNGTHW | RKGVFVSNGTYW | 137 | 15881 |
| HLA-B*15:03 | REGVFVSNGTH | RKGVFVSNGTY | 3026 | 27 |
| HLA-A*29:02 | GVFVSNGTH | GVFVSNGTY | 14554 | 136 |
| HLA-B*15:01 | GVFVSNGTH | GVFVSNGTY | 4414 | 70 |
| HLA-B*35:01 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 2599 | 42 |
| HLA-A*30:02 | GVFVSNGTH | GVFVSNGTY | 2307 | 38 |
| HLA-A*30:02 | REGVFVSNGTH | RKGVFVSNGTY | 3495 | 60 |
| HLA-B*15:03 | GVFVSNGTH | GVFVSNGTY | 2176 | 46 |
| HLA-B*15:01 | REGVFVSNGTH | RKGVFVSNGTY | 10166 | 217 |
| HLA-A*30:02 | PREGVFVSNGTH | PRKGVFVSNGTY | 10319 | 239 |
| HLA-B*15:01 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 12794 | 378 |
| HLA-A*30:02 | FPREGVFVSNGTH | FPRKGVFVSNGTY | 13269 | 467 |
| HLA-C*14:02 | VFVSNGTH | VFVSNGTY | 4648 | 168 |
| HLA-A*30:02 | HFPREGVFVSNGTH | HFPRKGVFVSNGTY | 12489 | 498 |
| HLA-B*58:01 | EGVFVSNGTHW | KGVFVSNGTYW | 1949 | 79 |
| HLA-A*24:02 | THWFVTQRNF | TYWFVTQRNF | 484 | 21 |
| HLA-C*03:02 | GVFVSNGTH | GVFVSNGTY | 8424 | 382 |
| HLA-A*23:01 | THWFVTQRNF | TYWFVTQRNF | 169 | 9 |
| HLA-B*57:01 | EGVFVSNGTHW | KGVFVSNGTYW | 2892 | 221 |
| HLA-A*68:02 | EGVFVSNGTHWFV | KGVFVSNGTYWFV | 355 | 4131 |
| HLA-C*14:02 | THWFVTQRNF | TYWFVTQRNF | 1544 | 137 |
| HLA-A*31:01 | THWFVTQR | TYWFVTQR | 1253 | 133 |
| HLA-A*33:03 | THWFVTQR | TYWFVTQR | 891 | 103 |
| HLA-A*29:02 | THWFVTQRNF | TYWFVTQRNF | 1545 | 189 |
| HLA-A*11:01 | NGTHWFVTQR | NGTYWFVTQR | 1084 | 160 |
| HLA-A*23:01 | GTHWFVTQRNF | GTYWFVTQRNF | 2154 | 318 |
| HLA-A*24:02 | HWFVTQRNF | YWFVTQRNF | 296 | 44 |
| HLA-A*03:01 | GTHWFVTQR | GTYWFVTQR | 511 | 76 |
| HLA-A*23:01 | HWFVTQRNF | YWFVTQRNF | 121 | 19 |
| HLA-B*15:03 | THWFVTQRNF | TYWFVTQRNF | 274 | 1741 |
| HLA-A*29:02 | HWFVTQRNF | YWFVTQRNF | 642 | 106 |
| HLA-A*29:02 | THWFVTQRNFY | TYWFVTQRNFY | 264 | 46 |
| HLA-C*14:02 | HWFVTQRNFY | YWFVTQRNFY | 551 | 97 |
| HLA-A*03:02 | NGTHWFVTQR | NGTYWFVTQR | 2297 | 451 |
| HLA-A*11:01 | SNGTHWFVTQR | SNGTYWFVTQR | 580 | 115 |
| HLA-A*29:02 | HWFVTQRNFY | YWFVTQRNFY | 42 | 9 |
| HLA-C*07:02 | HWFVTQRNF | YWFVTQRNF | 1677 | 394 |
| HLA-A*03:02 | GTHWFVTQR | GTYWFVTQR | 146 | 36 |
| HLA-C*14:02 | HWFVTQRNF | YWFVTQRNF | 68 | 19 |
| HLA-A*29:02 | GTHWFVTQRNFY | GTYWFVTQRNFY | 628 | 183 |
| HLA-A*74:01 | GTHWFVTQR | GTYWFVTQR | 175 | 51 |
| HLA-A*11:01 | GTHWFVTQR | GTYWFVTQR | 34 | 11 |
| HLA-A*02:06 | VSNGTHWFV | VSNGTYWFV | 106 | 35 |
| HLA-A*29:02 | VFVSNGTHWF | VFVSNGTYWF | 746 | 277 |
| HLA-A*68:01 | NGTHWFVTQRN | NGTYWFVTQRN | 1153 | 429 |
| HLA-A*02:01 | VSNGTHWFV | VSNGTYWFV | 617 | 235 |
| HLA-A*29:02 | FVSNGTHWF | FVSNGTYWF | 439 | 171 |
| HLA-A*68:01 | THWFVTQR | TYWFVTQR | 1103 | 440 |
| HLA-A*23:01 | VFVSNGTHWF | VFVSNGTYWF | 62 | 27 |
| HLA-B*15:03 | HWFVTQRNF | YWFVTQRNF | 539 | 236 |
| HLA-A*02:02 | VSNGTHWFV | VSNGTYWFV | 351 | 156 |
| HLA-C*03:03 | FVSNGTHWF | FVSNGTYWF | 137 | 308 |
| HLA-C*03:04 | FVSNGTHWF | FVSNGTYWF | 137 | 308 |
| HLA-A*68:02 | VSNGTHWFV | VSNGTYWFV | 95 | 43 |
| HLA-A*02:11 | FVSNGTHWF | FVSNGTYWF | 1048 | 482 |
| HLA-A*68:01 | VFVSNGTHWFVTQR | VFVSNGTYWFVTQR | 723 | 359 |
| HLA-A*02:11 | VSNGTHWFV | VSNGTYWFV | 42 | 21 |
| HLA-A*30:02 | GTHWFVTQRNFY | GTYWFVTQRNFY | 278 | 139 |
V1176F
Reference ELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQ
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated ELDKYFKNHTSPDVDLGDISGINASFVNIQKEIDRLNEVAKNLNESLIDLQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | DISGINASV | DISGINASF | 10 | 1289 |
| HLA-A*02:11 | GDISGINASV | GDISGINASF | 238 | 15161 |
| HLA-C*14:02 | SVVNIQKEI | SFVNIQKEI | 6508 | 124 |
| HLA-A*02:02 | GDISGINASV | GDISGINASF | 71 | 3625 |
| HLA-A*02:11 | LGDISGINASV | LGDISGINASF | 446 | 19525 |
| HLA-A*68:02 | DLGDISGINASV | DLGDISGINASF | 362 | 15753 |
| HLA-B*15:01 | GDISGINASV | GDISGINASF | 15806 | 370 |
| HLA-A*02:02 | LGDISGINASV | LGDISGINASF | 208 | 8728 |
| HLA-B*35:01 | DISGINASV | DISGINASF | 16065 | 447 |
| HLA-A*68:02 | GDISGINASV | GDISGINASF | 453 | 15954 |
| HLA-A*02:02 | VDLGDISGINASV | VDLGDISGINASF | 413 | 11912 |
| HLA-A*68:01 | SVVNIQKEIDR | SFVNIQKEIDR | 288 | 5363 |
| HLA-A*02:02 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 2908 | 166 |
| HLA-A*25:01 | DISGINASV | DISGINASF | 3057 | 204 |
| HLA-A*02:06 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 871 | 59 |
| HLA-A*26:01 | DISGINASV | DISGINASF | 1865 | 129 |
| HLA-A*02:11 | VVNIQKEIDRLNEV | FVNIQKEIDRLNEV | 2044 | 152 |
| HLA-A*68:01 | VVNIQKEIDR | FVNIQKEIDR | 1917 | 242 |
| HLA-A*02:06 | GINASVVNI | GINASFVNI | 1251 | 237 |
| HLA-A*68:01 | SGINASVVNIQK | SGINASFVNIQK | 913 | 194 |
| HLA-A*68:01 | NASVVNIQKEIDR | NASFVNIQKEIDR | 127 | 30 |
| HLA-A*68:01 | INASVVNIQK | INASFVNIQK | 249 | 62 |
| HLA-A*11:01 | ASVVNIQK | ASFVNIQK | 1597 | 479 |
| HLA-A*68:02 | DISGINASVV | DISGINASFV | 59 | 19 |
| HLA-A*03:02 | NASVVNIQK | NASFVNIQK | 949 | 325 |
| HLA-A*11:01 | NASVVNIQK | NASFVNIQK | 235 | 83 |
| HLA-A*11:01 | SGINASVVNIQK | SGINASFVNIQK | 411 | 150 |
| HLA-A*11:01 | INASVVNIQK | INASFVNIQK | 270 | 101 |
| HLA-A*03:02 | INASVVNIQK | INASFVNIQK | 840 | 335 |
| HLA-A*11:01 | ISGINASVVNIQK | ISGINASFVNIQK | 1056 | 450 |
| HLA-A*68:01 | NASVVNIQK | NASFVNIQK | 13 | 6 |
| HLA-C*16:01 | ISGINASVV | ISGINASFV | 368 | 787 |
N protein
203-204 RG->KR
Reference GGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | NSTPGSSRG | NSTPGSSKR | 32767 | 34 |
| HLA-A*33:03 | NSTPGSSRG | NSTPGSSKR | 32767 | 300 |
| HLA-B*15:03 | SRGTSPARM | SKRTSPARM | 2669 | 106 |
| HLA-A*31:01 | RNSTPGSSRG | RNSTPGSSKR | 7013 | 340 |
| HLA-A*30:01 | SSRNSTPGSSR | SSRNSTPGSSK | 356 | 20 |
| HLA-A*30:01 | GTSPARMAG | RTSPARMAG | 493 | 30 |
| HLA-A*31:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 3757 | 231 |
| HLA-A*30:01 | GTSPARMAGNGG | RTSPARMAGNGG | 6051 | 411 |
| HLA-A*31:01 | SSRNSTPGSSR | SSRNSTPGSSK | 127 | 1853 |
| HLA-A*30:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 4255 | 353 |
| HLA-A*30:01 | RNSTPGSSR | RNSTPGSSK | 2120 | 188 |
| HLA-A*31:01 | RNSTPGSSR | RNSTPGSSK | 123 | 1335 |
| HLA-A*03:01 | SSRNSTPGSSR | SSRNSTPGSSK | 4753 | 442 |
| HLA-A*11:01 | SSRNSTPGSSR | SSRNSTPGSSK | 2231 | 263 |
| HLA-A*68:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 270 | 1385 |
| HLA-A*30:01 | GSSRGTSPA | GSSKRTSPA | 555 | 145 |
| HLA-A*30:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 1625 | 469 |
| HLA-A*30:01 | SSRGTSPARMAG | SSKRTSPARMAG | 449 | 1031 |
NS9c protein
G50N
Reference AAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||||
Mutated AAVKPLLVPHHVVATVQEIQLQAAVNELLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*18:01 | GELLLLEWLAM | NELLLLEWLAM | 4979 | 247 |
| HLA-B*18:01 | GELLLLEW | NELLLLEW | 3915 | 401 |
| HLA-B*40:01 | GELLLLEWL | NELLLLEWL | 28 | 140 |
| HLA-A*02:11 | QLQAAVGEL | QLQAAVNEL | 120 | 27 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVNEL | 338 | 81 |
| HLA-B*40:02 | GELLLLEWL | NELLLLEWL | 52 | 204 |
| HLA-B*40:02 | GELLLLEWLAM | NELLLLEWLAM | 201 | 772 |
| HLA-A*02:01 | QLQAAVGEL | QLQAAVNEL | 1290 | 358 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVNEL | 92 | 28 |
| HLA-B*58:01 | VGELLLLEW | VNELLLLEW | 330 | 1071 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVNEL | 170 | 58 |
| HLA-A*02:01 | LQAAVGELL | LQAAVNELL | 938 | 352 |
| HLA-A*02:02 | LQAAVGELL | LQAAVNELL | 44 | 17 |
| HLA-B*40:06 | GELLLLEWLA | NELLLLEWLA | 268 | 677 |
| HLA-B*40:02 | GELLLLEWLA | NELLLLEWLA | 475 | 1195 |
| HLA-A*02:11 | LQAAVGELL | LQAAVNELL | 386 | 154 |
| HLA-A*02:01 | IQLQAAVGEL | IQLQAAVNEL | 1138 | 475 |
| HLA-A*02:01 | QLQAAVGELL | QLQAAVNELL | 961 | 403 |
| HLA-A*02:01 | GELLLLEWLAMA | NELLLLEWLAMA | 109 | 258 |
| HLA-A*02:02 | QLQAAVGELL | QLQAAVNELL | 35 | 15 |
| HLA-A*02:02 | IQLQAAVGELL | IQLQAAVNELL | 724 | 313 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVNELL | 303 | 131 |
| HLA-B*58:01 | AVGELLLLEW | AVNELLLLEW | 196 | 87 |
| HLA-A*02:11 | GELLLLEWLAMA | NELLLLEWLAMA | 29 | 61 |
| HLA-B*57:01 | AVGELLLLEW | AVNELLLLEW | 432 | 206 |
| HLA-A*68:02 | EIQLQAAVGELL | EIQLQAAVNELL | 697 | 346 |
NSP3 protein
D736G
Reference KRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated KRGDKSVYYTSNPTTFHLDGEVITFGNLKTLLSLREVRTIKVFTTVDNINL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*04:01 | TFDNLKTLL | TFGNLKTLL | 226 | 13025 |
| HLA-B*08:01 | FDNLKTLL | FGNLKTLL | 5539 | 456 |
| HLA-B*08:01 | FDNLKTLLSL | FGNLKTLLSL | 2672 | 260 |
| HLA-A*31:01 | DNLKTLLSLR | GNLKTLLSLR | 941 | 173 |
| HLA-A*03:01 | GEVITFDNLK | GEVITFGNLK | 1301 | 243 |
| HLA-A*33:03 | DNLKTLLSLR | GNLKTLLSLR | 131 | 446 |
| HLA-A*02:11 | ITFDNLKTL | ITFGNLKTL | 135 | 367 |
| HLA-A*11:01 | GEVITFDNLK | GEVITFGNLK | 103 | 39 |
| HLA-A*11:01 | EVITFDNLK | EVITFGNLK | 352 | 139 |
| HLA-A*11:01 | HLDGEVITFDNLK | HLDGEVITFGNLK | 1099 | 478 |
| HLA-A*03:02 | GEVITFDNLK | GEVITFGNLK | 407 | 193 |
S1807F
Reference STFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated STFNVPMEKLKTLVATAEAELAKNVFLDNVLSTFISAARQGFVDSDVETKD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*58:01 | VSLDNVLSTF | VFLDNVLSTF | 27 | 5289 |
| HLA-B*57:01 | VSLDNVLSTF | VFLDNVLSTF | 71 | 9670 |
| HLA-B*44:02 | AEAELAKNVS | AEAELAKNVF | 1684 | 13 |
| HLA-B*44:03 | AEAELAKNVS | AEAELAKNVF | 2231 | 18 |
| HLA-B*40:01 | AEAELAKNVS | AEAELAKNVF | 8476 | 112 |
| HLA-A*23:01 | VSLDNVLSTF | VFLDNVLSTF | 883 | 12 |
| HLA-C*14:02 | VSLDNVLSTF | VFLDNVLSTF | 3791 | 57 |
| HLA-A*24:02 | VSLDNVLSTF | VFLDNVLSTF | 2274 | 37 |
| HLA-B*15:03 | AEAELAKNVS | AEAELAKNVF | 23319 | 437 |
| HLA-B*40:02 | AEAELAKNVS | AEAELAKNVF | 2792 | 114 |
| HLA-A*23:01 | NVSLDNVLSTF | NVFLDNVLSTF | 10817 | 444 |
| HLA-A*29:02 | VSLDNVLSTF | VFLDNVLSTF | 6418 | 423 |
| HLA-A*02:01 | SLDNVLSTF | FLDNVLSTF | 1891 | 131 |
| HLA-C*03:03 | SLDNVLSTF | FLDNVLSTF | 4723 | 329 |
| HLA-C*03:04 | SLDNVLSTF | FLDNVLSTF | 4723 | 329 |
| HLA-A*02:11 | SLDNVLST | FLDNVLST | 3788 | 264 |
| HLA-A*02:06 | SLDNVLSTFI | FLDNVLSTFI | 307 | 22 |
| HLA-B*35:01 | SLDNVLSTF | FLDNVLSTF | 3897 | 287 |
| HLA-C*03:02 | SLDNVLSTF | FLDNVLSTF | 920 | 69 |
| HLA-C*16:01 | VSLDNVLSTF | VFLDNVLSTF | 381 | 4609 |
| HLA-A*02:06 | SLDNVLSTF | FLDNVLSTF | 1267 | 112 |
| HLA-A*02:02 | SLDNVLSTF | FLDNVLSTF | 952 | 85 |
| HLA-C*08:01 | SLDNVLSTF | FLDNVLSTF | 4593 | 414 |
| HLA-A*02:02 | VSLDNVLSTFI | VFLDNVLSTFI | 1936 | 188 |
| HLA-A*02:01 | VSLDNVLSTFI | VFLDNVLSTFI | 2854 | 278 |
| HLA-A*02:01 | SLDNVLSTFI | FLDNVLSTFI | 78 | 8 |
| HLA-A*02:11 | SLDNVLSTFIS | FLDNVLSTFIS | 1152 | 121 |
| HLA-C*03:02 | VSLDNVLSTF | VFLDNVLSTF | 201 | 1898 |
| HLA-A*02:11 | VSLDNVLSTFI | VFLDNVLSTFI | 593 | 63 |
| HLA-A*02:11 | SLDNVLSTF | FLDNVLSTF | 353 | 38 |
| HLA-A*02:01 | SLDNVLSTFIS | FLDNVLSTFIS | 3723 | 409 |
| HLA-C*05:01 | SLDNVLSTFI | FLDNVLSTFI | 515 | 57 |
| HLA-A*02:11 | NVSLDNVLSTFI | NVFLDNVLSTFI | 1761 | 201 |
| HLA-A*02:11 | SLDNVLSTFISAA | FLDNVLSTFISAA | 408 | 49 |
| HLA-A*02:02 | SLDNVLSTFIS | FLDNVLSTFIS | 3121 | 380 |
| HLA-A*02:01 | SLDNVLSTFISAA | FLDNVLSTFISAA | 1298 | 159 |
| HLA-C*12:02 | SLDNVLSTF | FLDNVLSTF | 3581 | 446 |
| HLA-C*17:01 | SLDNVLSTFI | FLDNVLSTFI | 3839 | 492 |
| HLA-A*02:02 | SLDNVLSTFI | FLDNVLSTFI | 35 | 5 |
| HLA-A*02:02 | SLDNVLSTFISAA | FLDNVLSTFISAA | 307 | 47 |
| HLA-C*17:01 | SLDNVLSTF | FLDNVLSTF | 812 | 133 |
| HLA-A*02:02 | SLDNVLSTFISA | FLDNVLSTFISA | 526 | 90 |
| HLA-A*02:06 | SLDNVLSTFISAA | FLDNVLSTFISAA | 2529 | 465 |
| HLA-A*02:02 | NVSLDNVLSTFI | NVFLDNVLSTFI | 2076 | 405 |
| HLA-B*45:01 | AEAELAKNVS | AEAELAKNVF | 173 | 848 |
| HLA-C*08:02 | SLDNVLSTF | FLDNVLSTF | 157 | 34 |
| HLA-B*15:01 | VSLDNVLSTF | VFLDNVLSTF | 279 | 1226 |
| HLA-A*02:11 | SLDNVLSTFISA | FLDNVLSTFISA | 100 | 23 |
| HLA-A*02:11 | SLDNVLSTFI | FLDNVLSTFI | 13 | 3 |
| HLA-A*02:01 | SLDNVLSTFISA | FLDNVLSTFISA | 203 | 49 |
| HLA-A*68:02 | NVSLDNVLSTFI | NVFLDNVLSTFI | 1180 | 286 |
| HLA-C*05:01 | SLDNVLSTF | FLDNVLSTF | 73 | 19 |
| HLA-B*15:03 | VSLDNVLSTF | VFLDNVLSTF | 157 | 493 |
| HLA-C*14:02 | SLDNVLSTF | FLDNVLSTF | 1131 | 400 |
| HLA-A*02:06 | SLDNVLSTFISA | FLDNVLSTFISA | 289 | 108 |
| HLA-C*05:01 | VSLDNVLSTF | VFLDNVLSTF | 256 | 656 |
| HLA-B*40:02 | AEAELAKNVSL | AEAELAKNVFL | 87 | 210 |
| HLA-B*40:01 | AEAELAKNVSL | AEAELAKNVFL | 33 | 72 |
| HLA-B*15:03 | SLDNVLSTF | FLDNVLSTF | 176 | 81 |
| HLA-B*40:02 | AELAKNVSL | AELAKNVFL | 21 | 43 |
NSP4 protein
D217N
Reference VLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWV
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VLMDGSIIQFPNTYLEGSVRVVTTFNSEYCRHGTCERSEAGVCVSTSGRWV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | SVRVVTTFD | SVRVVTTFN | 276 | 44 |
| HLA-C*16:01 | VVTTFDSEY | VVTTFNSEY | 381 | 152 |
| HLA-A*31:01 | TTFDSEYCR | TTFNSEYCR | 52 | 21 |
| HLA-A*31:01 | VTTFDSEYCR | VTTFNSEYCR | 192 | 82 |
| HLA-C*12:03 | VVTTFDSEY | VVTTFNSEY | 881 | 380 |
| HLA-A*31:01 | VVTTFDSEYCR | VVTTFNSEYCR | 398 | 174 |
L438P
Reference EEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSY
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated EEAALCTFLLNKEMYLKLRSDVLLPPTQYNRYLALYNKYKYFSGAMDTTSY
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | KLRSDVLLPL | KLRSDVLLPP | 5 | 1146 |
| HLA-C*06:02 | LRSDVLLPL | LRSDVLLPP | 103 | 17926 |
| HLA-A*02:11 | YLKLRSDVLLPL | YLKLRSDVLLPP | 5 | 788 |
| HLA-A*02:11 | LKLRSDVLLPL | LKLRSDVLLPP | 93 | 14621 |
| HLA-C*07:02 | LRSDVLLPL | LRSDVLLPP | 104 | 15467 |
| HLA-C*07:01 | LRSDVLLPL | LRSDVLLPP | 104 | 15210 |
| HLA-A*02:01 | KLRSDVLLPL | KLRSDVLLPP | 10 | 1434 |
| HLA-A*02:02 | KLRSDVLLPL | KLRSDVLLPP | 6 | 686 |
| HLA-A*02:11 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 82 | 9276 |
| HLA-A*02:02 | LKLRSDVLLPL | LKLRSDVLLPP | 120 | 12620 |
| HLA-A*02:01 | LKLRSDVLLPL | LKLRSDVLLPP | 161 | 15657 |
| HLA-A*02:02 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 49 | 4677 |
| HLA-A*02:02 | LPLTQYNRYL | LPPTQYNRYL | 241 | 20700 |
| HLA-B*08:01 | YLKLRSDVLLPL | YLKLRSDVLLPP | 13 | 1103 |
| HLA-A*02:01 | YLKLRSDVLLPL | YLKLRSDVLLPP | 11 | 889 |
| HLA-A*02:02 | YLKLRSDVLLPL | YLKLRSDVLLPP | 6 | 417 |
| HLA-A*02:06 | KLRSDVLLPL | KLRSDVLLPP | 29 | 1825 |
| HLA-A*02:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 161 | 9143 |
| HLA-C*14:02 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 313 | 17335 |
| HLA-C*08:02 | RSDVLLPL | RSDVLLPP | 400 | 21753 |
| HLA-B*27:05 | LRSDVLLPL | LRSDVLLPP | 81 | 4120 |
| HLA-B*15:03 | LRSDVLLPL | LRSDVLLPP | 149 | 6800 |
| HLA-B*27:05 | KLRSDVLLPL | KLRSDVLLPP | 94 | 4082 |
| HLA-A*02:06 | YLKLRSDVLLPL | YLKLRSDVLLPP | 35 | 1507 |
| HLA-A*02:01 | KLRSDVLLPLT | KLRSDVLLPPT | 144 | 5473 |
| HLA-A*02:02 | KLRSDVLLPLT | KLRSDVLLPPT | 183 | 6897 |
| HLA-B*08:01 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 231 | 8566 |
| HLA-B*38:01 | LRSDVLLPL | LRSDVLLPP | 499 | 18496 |
| HLA-A*02:11 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 400 | 14119 |
| HLA-B*08:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 151 | 5303 |
| HLA-B*27:05 | YLKLRSDVLLPL | YLKLRSDVLLPP | 270 | 9416 |
| HLA-A*32:01 | KLRSDVLLPL | KLRSDVLLPP | 418 | 14267 |
| HLA-A*68:02 | LTQYNRYLA | PTQYNRYLA | 349 | 11388 |
| HLA-A*02:02 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 82 | 2621 |
| HLA-A*02:02 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 322 | 9290 |
| HLA-C*16:01 | LTQYNRYLAL | PTQYNRYLAL | 285 | 8211 |
| HLA-B*27:05 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 378 | 10377 |
| HLA-A*02:11 | KLRSDVLLPLT | KLRSDVLLPPT | 114 | 2763 |
| HLA-A*23:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 466 | 10083 |
| HLA-B*15:01 | LTQYNRYLAL | PTQYNRYLAL | 125 | 2585 |
| HLA-B*15:01 | KLRSDVLLPL | KLRSDVLLPP | 335 | 6450 |
| HLA-A*30:01 | LTQYNRYLA | PTQYNRYLA | 171 | 3143 |
| HLA-A*02:01 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 177 | 2183 |
| HLA-B*15:03 | LTQYNRYLAL | PTQYNRYLAL | 15 | 177 |
| HLA-B*53:01 | LPLTQYNRY | LPPTQYNRY | 497 | 4773 |
| HLA-A*02:06 | LTQYNRYLAL | PTQYNRYLAL | 483 | 4423 |
| HLA-A*11:01 | LTQYNRYLALYNK | PTQYNRYLALYNK | 388 | 3496 |
| HLA-A*02:11 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 115 | 1005 |
| HLA-B*35:01 | LPLTQYNRY | LPPTQYNRY | 66 | 540 |
| HLA-B*08:01 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 258 | 1470 |
| HLA-B*08:01 | LTQYNRYLAL | PTQYNRYLAL | 126 | 711 |
| HLA-A*01:01 | LTQYNRYLALY | PTQYNRYLALY | 400 | 1559 |
| HLA-A*02:11 | LLPLTQYNRYL | LLPPTQYNRYL | 389 | 105 |
| HLA-A*30:01 | KLRSDVLLPL | KLRSDVLLPP | 108 | 390 |
| HLA-A*29:02 | LTQYNRYLALY | PTQYNRYLALY | 212 | 592 |
| HLA-A*02:02 | LLPLTQYNRYL | LLPPTQYNRYL | 400 | 145 |
| HLA-A*02:01 | VLLPLTQYNRYLAL | VLLPPTQYNRYLAL | 343 | 922 |
| HLA-A*02:01 | VLLPLTQYNRYL | VLLPPTQYNRYL | 170 | 445 |
| HLA-A*30:02 | LTQYNRYLALY | PTQYNRYLALY | 92 | 211 |
| HLA-A*02:02 | VLLPLTQYNRYL | VLLPPTQYNRYL | 147 | 335 |
| HLA-B*42:01 | LPLTQYNRYL | LPPTQYNRYL | 72 | 154 |
| HLA-A*02:02 | VLLPLTQYNRYLAL | VLLPPTQYNRYLAL | 381 | 810 |
| HLA-A*29:02 | VLLPLTQYNRY | VLLPPTQYNRY | 143 | 302 |
NSP7 protein
L71F
Reference TEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQ
||||||||||||||||||||||||| ||||||||||||
Mutated TEAFEKMVSLLSVLLSMQGAVDINKFCEEMLDNRATLQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | SMQGAVDINKL | SMQGAVDINKF | 382 | 10562 |
| HLA-B*15:03 | MQGAVDINKL | MQGAVDINKF | 1284 | 60 |
| HLA-A*02:02 | MQGAVDINKL | MQGAVDINKF | 359 | 7270 |
| HLA-B*15:01 | MQGAVDINKL | MQGAVDINKF | 1395 | 73 |
| HLA-A*02:11 | LCEEMLDNRATL | FCEEMLDNRATL | 709 | 64 |
| HLA-B*58:01 | LSMQGAVDINKL | LSMQGAVDINKF | 2789 | 298 |
| HLA-A*02:02 | LCEEMLDNRATL | FCEEMLDNRATL | 1191 | 137 |
| HLA-A*02:01 | LCEEMLDNRATL | FCEEMLDNRATL | 2490 | 324 |
| HLA-A*02:02 | KLCEEMLDNRATL | KFCEEMLDNRATL | 278 | 1870 |
| HLA-A*02:11 | KLCEEMLDNRATL | KFCEEMLDNRATL | 188 | 723 |
| HLA-A*31:01 | KLCEEMLDNR | KFCEEMLDNR | 673 | 240 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |
NSP13 protein
L280F
Reference TLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIV
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Mutated TLNISDEFSSNVANYQKVGMQKYSTFQGPPGTGKSHFAIGLALYYPSARIV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | VGMQKYSTL | VGMQKYSTF | 927 | 41 |
| HLA-B*15:01 | KVGMQKYSTL | KVGMQKYSTF | 3133 | 163 |
| HLA-A*03:01 | TLQGPPGTGK | TFQGPPGTGK | 119 | 2170 |
| HLA-B*15:03 | KVGMQKYSTL | KVGMQKYSTF | 3689 | 214 |
| HLA-B*15:03 | YQKVGMQKYSTL | YQKVGMQKYSTF | 253 | 16 |
| HLA-B*15:01 | YQKVGMQKYSTL | YQKVGMQKYSTF | 512 | 33 |
| HLA-A*23:01 | VGMQKYSTL | VGMQKYSTF | 4632 | 379 |
| HLA-A*03:02 | TLQGPPGTGK | TFQGPPGTGK | 147 | 1741 |
| HLA-B*15:03 | QKVGMQKYSTL | QKVGMQKYSTF | 3733 | 322 |
| HLA-B*15:03 | GMQKYSTL | GMQKYSTF | 5092 | 464 |
| HLA-B*15:03 | NYQKVGMQKYSTL | NYQKVGMQKYSTF | 4567 | 446 |
| HLA-A*68:01 | STLQGPPGTGK | STFQGPPGTGK | 1382 | 141 |
| HLA-A*24:02 | NYQKVGMQKYSTL | NYQKVGMQKYSTF | 1328 | 141 |
| HLA-A*23:01 | NYQKVGMQKYSTL | NYQKVGMQKYSTF | 1415 | 152 |
| HLA-B*08:01 | YQKVGMQKYSTL | YQKVGMQKYSTF | 121 | 733 |
| HLA-B*08:01 | VGMQKYSTL | VGMQKYSTF | 57 | 342 |
| HLA-C*03:02 | VGMQKYSTL | VGMQKYSTF | 970 | 208 |
| HLA-A*32:01 | KVGMQKYSTL | KVGMQKYSTF | 809 | 194 |
| HLA-A*11:01 | TLQGPPGTGK | TFQGPPGTGK | 326 | 1321 |
| HLA-C*03:03 | VGMQKYSTL | VGMQKYSTF | 330 | 1283 |
| HLA-C*03:04 | VGMQKYSTL | VGMQKYSTF | 330 | 1283 |
| HLA-B*08:01 | KVGMQKYSTL | KVGMQKYSTF | 458 | 1769 |
| HLA-A*03:01 | STLQGPPGTGK | STFQGPPGTGK | 284 | 82 |
| HLA-A*30:01 | STLQGPPGTGK | STFQGPPGTGK | 1558 | 466 |
| HLA-A*68:01 | YSTLQGPPGTGK | YSTFQGPPGTGK | 1177 | 494 |
| HLA-A*03:02 | STLQGPPGTGK | STFQGPPGTGK | 428 | 199 |
| HLA-B*15:01 | MQKYSTLQG | MQKYSTFQG | 306 | 617 |
| HLA-A*11:01 | YSTLQGPPGTGK | YSTFQGPPGTGK | 557 | 277 |
A368V
Reference FDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRA
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Mutated FDKFKVNSTLEQYVFCTVNALPETTVDIVVFDEISMATNYDLSVVNARLRA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | YVFCTVNALPETTA | YVFCTVNALPETTV | 2317 | 156 |
| HLA-A*68:02 | TVNALPETTA | TVNALPETTV | 2375 | 204 |
| HLA-A*68:02 | YVFCTVNALPETTA | YVFCTVNALPETTV | 2743 | 468 |
| HLA-A*02:06 | YVFCTVNALPETTA | YVFCTVNALPETTV | 1022 | 197 |
| HLA-A*68:02 | TADIVVFDEI | TVDIVVFDEI | 801 | 158 |
| HLA-A*02:06 | CTVNALPETTA | CTVNALPETTV | 1733 | 348 |
| HLA-A*02:02 | YVFCTVNALPETTA | YVFCTVNALPETTV | 2115 | 432 |
| HLA-A*26:01 | ETTADIVVF | ETTVDIVVF | 179 | 618 |
| HLA-A*68:02 | TTADIVVFDEISM | TTVDIVVFDEISM | 140 | 467 |
| HLA-A*68:02 | TTADIVVFDEI | TTVDIVVFDEI | 26 | 81 |
| HLA-A*25:01 | ETTADIVVF | ETTVDIVVF | 388 | 1063 |
| HLA-A*68:02 | TTADIVVFDEISMA | TTVDIVVFDEISMA | 89 | 220 |
| HLA-A*68:02 | TTADIVVFDEIS | TTVDIVVFDEIS | 432 | 998 |