Summary

This strain contains 49 protein-level mutations:

• 26 mutations in Spike protein: T19I, G142D, V213G, G339D, S371F, S373P, 375-376 ST->FA, D405N, R408S, K417N, N440K, L452R, 477-478 ST->NK, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K
• 4 mutations in N protein: P13L, R32C, 203-204 RG->KR, S413R
• 3 mutations in M protein: D3N, Q19E, A63T
• 1 mutation in E protein: T9I
• 1 mutation in NS3 protein: T223I
• 1 mutation in NS9b protein: P10S
• 1 mutation in NS9c protein: G50N
• 1 mutation in NSP1 protein: S135R
• 2 mutations in NSP3 protein: T24I, G489S
• 3 mutations in NSP4 protein: L264F, T327I, T492I
• 1 mutation in NSP5 protein: P132H
• 1 mutation in NSP6 protein: F108L
• 1 mutation in NSP12 protein: P323L
• 2 mutations in NSP13 protein: R392C, T481M
• 1 mutation in NSP15 protein: T112I

---

We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.

Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:

1. Tight binding (IC₅₀ affinity ≤ 50 nM)
2. Moderate binding (50 nM < IC₅₀ affinity ≤ 500 nM)
3. Weak/no binding (IC₅₀ affinity > 500 nM)

Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.

The total number of HLA-peptide interactions affected by the mutations

For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).

While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC₅₀ affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.

Spike protein

T19I

Reference  MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFR
           |||||||||||||||||| |||||||||||||||||||||||||
Mutated    MFVFLVLLPLVSSQCVNLITRTQLPPAYTNSFTRGVYYPDKVFR

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*58:01	VSSQCVNLT	VSSQCVNLI	3986	159
HLA-C*15:02	VSSQCVNLT	VSSQCVNLI	10208	479
HLA-A*02:02	LVSSQCVNLT	LVSSQCVNLI	3376	271
HLA-A*02:11	VLLPLVSSQCVNLT	VLLPLVSSQCVNLI	4460	394
HLA-A*02:11	NLTTRTQLPPA	NLITRTQLPPA	4965	468
HLA-B*08:01	NLTTRTQL	NLITRTQL	531	100
HLA-B*15:03	TTRTQLPPAY	ITRTQLPPAY	888	426

G142D

Reference  LLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCT
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    LLIVNNATNVVIKVCEFQFCNDPFLDVYYHKNNKSWMESEFRVYSSANNCT

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*03:01	GVYYHKNNK	DVYYHKNNK	27	2841
HLA-A*30:01	GVYYHKNNK	DVYYHKNNK	51	3035
HLA-A*11:01	GVYYHKNNK	DVYYHKNNK	51	1768
HLA-A*03:02	GVYYHKNNK	DVYYHKNNK	119	3887
HLA-A*31:01	GVYYHKNNK	DVYYHKNNK	375	5203
HLA-A*68:01	GVYYHKNNK	DVYYHKNNK	363	28
HLA-A*68:02	FCNDPFLGV	FCNDPFLDV	281	3555
HLA-A*33:03	GVYYHKNNK	DVYYHKNNK	3253	287
HLA-A*02:06	FCNDPFLGV	FCNDPFLDV	116	1132
HLA-A*02:11	FCNDPFLGV	FCNDPFLDV	87	751
HLA-A*02:06	EFQFCNDPFLGV	EFQFCNDPFLDV	87	554
HLA-A*30:02	CNDPFLGVY	CNDPFLDVY	310	1959
HLA-A*02:02	FCNDPFLGV	FCNDPFLDV	365	2187
HLA-A*02:06	FQFCNDPFLGVY	FQFCNDPFLDVY	135	784
HLA-A*30:02	FCNDPFLGVY	FCNDPFLDVY	408	1738
HLA-A*02:11	EFQFCNDPFLGV	EFQFCNDPFLDV	334	1214
HLA-A*02:02	EFQFCNDPFLGV	EFQFCNDPFLDV	477	1725
HLA-A*02:11	FQFCNDPFLGVY	FQFCNDPFLDVY	488	1737
HLA-A*02:06	FQFCNDPFLGV	FQFCNDPFLDV	7	21
HLA-A*02:11	FQFCNDPFLGV	FQFCNDPFLDV	17	49
HLA-A*02:01	FQFCNDPFLGV	FQFCNDPFLDV	39	111
HLA-A*30:02	FQFCNDPFLGVY	FQFCNDPFLDVY	336	923
HLA-A*02:02	FQFCNDPFLGV	FQFCNDPFLDV	45	122
HLA-B*15:01	FQFCNDPFLGVY	FQFCNDPFLDVY	28	56

V213G

Reference  NLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRF
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    NLREFVFKNIDGYFKIYSKHTPINLGRDLPQGFSALEPLVDLPIGINITRF

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:02	YSKHTPINLV	YSKHTPINLG	199	16298
HLA-A*02:11	KIYSKHTPINLV	KIYSKHTPINLG	104	6984
HLA-B*15:01	LVRDLPQGF	LGRDLPQGF	160	2486
HLA-C*01:02	VRDLPQGFSAL	GRDLPQGFSAL	431	3580
HLA-C*03:02	LVRDLPQGF	LGRDLPQGF	259	1765
HLA-B*15:03	LVRDLPQGF	LGRDLPQGF	254	793
HLA-B*07:02	HTPINLVRDL	HTPINLGRDL	892	311
HLA-B*27:05	VRDLPQGFSA	GRDLPQGFSA	1057	375
HLA-A*68:01	YSKHTPINLVR	YSKHTPINLGR	362	130
HLA-B*07:02	TPINLVRDL	TPINLGRDL	213	81
HLA-A*68:01	HTPINLVR	HTPINLGR	480	202
HLA-A*33:03	YSKHTPINLVR	YSKHTPINLGR	762	337

G339D

Reference  QTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVAD
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    QTSNFRVQPTESIVRFPNITNLCPFDEVFNATRFASVYAWNRKRISNCVAD

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*40:01	GEVFNATRF	DEVFNATRF	164	7112
HLA-B*18:01	GEVFNATRFASVY	DEVFNATRFASVY	2165	64
HLA-B*18:01	GEVFNATRF	DEVFNATRF	971	32
HLA-B*50:01	GEVFNATRFA	DEVFNATRFA	147	4233
HLA-B*40:02	GEVFNATRF	DEVFNATRF	283	7625
HLA-B*40:06	GEVFNATRFA	DEVFNATRFA	201	5089
HLA-B*15:03	GEVFNATRF	DEVFNATRF	359	4698
HLA-A*68:01	FGEVFNATR	FDEVFNATR	338	2409
HLA-B*45:01	GEVFNATRFA	DEVFNATRFA	116	688
HLA-B*44:02	GEVFNATRF	DEVFNATRF	158	889
HLA-B*44:03	GEVFNATRF	DEVFNATRF	136	673
HLA-B*15:01	TNLCPFGEVF	TNLCPFDEVF	368	1059
HLA-B*15:03	TNLCPFGEVF	TNLCPFDEVF	442	1176
HLA-B*15:03	NLCPFGEVF	NLCPFDEVF	130	341
HLA-A*68:02	GEVFNATRFA	DEVFNATRFA	879	342
HLA-B*15:01	NLCPFGEVF	NLCPFDEVF	315	773

S371F

Reference  RFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVY
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    RFASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVY

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*32:01	VLYNSASFS	VLYNFAPFF	22186	33
HLA-A*23:01	VLYNSASFS	VLYNFAPFF	32767	84
HLA-B*15:03	VLYNSASFS	VLYNFAPFF	3280	11
HLA-A*29:02	VLYNSASFS	VLYNFAPFF	10439	66
HLA-A*23:01	LYNSASFS	LYNFAPFF	22508	183
HLA-C*05:01	VADYSVLYNS	VADYSVLYNF	11802	122
HLA-A*24:02	LYNSASFS	LYNFAPFF	25164	266
HLA-A*32:01	SVLYNSASFS	SVLYNFAPFF	14340	178
HLA-A*24:02	VLYNSASFS	VLYNFAPFF	32767	429
HLA-A*29:02	SVLYNSASFS	SVLYNFAPFF	16185	256
HLA-B*15:01	VLYNSASFS	VLYNFAPFF	8189	139
HLA-C*03:02	VLYNSASFS	VLYNFAPFF	13004	222
HLA-C*03:02	VADYSVLYNS	VADYSVLYNF	20606	367
HLA-A*23:01	SVLYNSASFS	SVLYNFAPFF	24524	482
HLA-C*14:02	LYNSASFS	LYNFAPFF	5693	117
HLA-B*58:01	VADYSVLYNS	VADYSVLYNF	6124	129
HLA-B*15:03	SVLYNSASFS	SVLYNFAPFF	2850	70
HLA-A*01:01	NSASFSTFKCY	NFAPFFAFKCY	328	13051
HLA-A*23:01	DYSVLYNSASFS	DYSVLYNFAPFF	14260	374
HLA-C*16:01	NSASFSTF	NFAPFFAF	243	8562
HLA-C*08:02	VADYSVLYNS	VADYSVLYNF	16435	470
HLA-A*02:11	SVLYNSASFST	SVLYNFAPFFA	3830	120
HLA-C*14:02	VLYNSASFS	VLYNFAPFF	11085	354
HLA-A*02:01	VLYNSASFST	VLYNFAPFFA	243	8
HLA-B*57:01	VADYSVLYNS	VADYSVLYNF	11273	384
HLA-A*23:01	NSASFSTF	NFAPFFAF	12240	424
HLA-A*29:02	YNSASFSTF	YNFAPFFAF	1671	60
HLA-B*58:01	YSVLYNSASFS	YSVLYNFAPFF	6920	296
HLA-A*68:02	NSASFSTFKCYGV	NFAPFFAFKCYGV	48	1043
HLA-C*14:02	NSASFSTF	NFAPFFAF	2544	120
HLA-B*15:01	SVLYNSASFS	SVLYNFAPFF	2838	139
HLA-A*02:02	VLYNSASFST	VLYNFAPFFA	202	10
HLA-C*03:02	SVLYNSASFS	SVLYNFAPFF	8888	443
HLA-A*02:06	VLYNSASFST	VLYNFAPFFA	710	36
HLA-B*15:03	YSVLYNSASFS	YSVLYNFAPFF	3170	164
HLA-A*02:11	VLYNSASFST	VLYNFAPFFA	77	4
HLA-A*02:01	SVLYNSASFST	SVLYNFAPFFA	5674	301
HLA-A*11:01	LYNSASFSTFK	LYNFAPFFAFK	43	797
HLA-B*40:02	ADYSVLYNS	ADYSVLYNF	4645	259
HLA-A*30:02	SASFSTFKCY	FAPFFAFKCY	71	1241
HLA-A*02:11	YSVLYNSASFST	YSVLYNFAPFFA	7859	469
HLA-A*32:01	YNSASFSTF	YNFAPFFAF	4333	294
HLA-A*33:03	YNSASFSTFK	YNFAPFFAFK	823	57
HLA-A*02:06	SVLYNSASFST	SVLYNFAPFFA	4097	319
HLA-B*18:01	YNSASFSTF	YNFAPFFAF	3495	284
HLA-A*33:03	NSASFSTFK	NFAPFFAFK	181	16
HLA-A*02:11	VLYNSASFSTF	VLYNFAPFFAF	2537	236
HLA-A*11:01	NSASFSTFK	NFAPFFAFK	9	94
HLA-A*29:02	NSASFSTFKCY	NFAPFFAFKCY	662	64
HLA-A*29:02	YNSASFSTFKCY	YNFAPFFAFKCY	842	86
HLA-A*02:01	VLYNSASFSTF	VLYNFAPFFAF	4199	455
HLA-C*03:02	YSVLYNSASFS	YSVLYNFAPFF	2405	264
HLA-A*02:06	SVLYNSASF	SVLYNFAPF	2112	234
HLA-B*15:01	VLYNSASFSTF	VLYNFAPFFAF	50	420
HLA-A*31:01	YNSASFSTFK	YNFAPFFAFK	607	73
HLA-A*30:01	LYNSASFST	LYNFAPFFA	3467	433
HLA-A*33:03	SVLYNSASFSTFK	SVLYNFAPFFAFK	2778	357
HLA-B*35:01	SASFSTFKCY	FAPFFAFKCY	490	65
HLA-A*31:01	NSASFSTFK	NFAPFFAFK	293	47
HLA-A*29:02	LYNSASFSTF	LYNFAPFFAF	387	63
HLA-A*30:02	VLYNSASFS	VLYNFAPFF	2919	476
HLA-A*31:01	SVLYNSASFSTFK	SVLYNFAPFFAFK	857	148
HLA-A*23:01	YNSASFSTF	YNFAPFFAF	352	63
HLA-A*03:01	LYNSASFSTFK	LYNFAPFFAFK	295	1614
HLA-A*23:01	SVLYNSASF	SVLYNFAPF	990	197
HLA-A*02:11	SVLYNSASFS	SVLYNFAPFF	2012	435
HLA-A*03:01	NSASFSTFK	NFAPFFAFK	113	494
HLA-B*35:01	YNSASFSTF	YNFAPFFAF	760	180
HLA-A*03:02	LYNSASFSTFK	LYNFAPFFAFK	202	831
HLA-A*02:01	VLYNSASFS	VLYNFAPFF	1548	377
HLA-A*02:11	VLYNSASFS	VLYNFAPFF	258	63
HLA-A*01:01	SASFSTFKCY	FAPFFAFKCY	461	1849
HLA-A*11:01	SASFSTFK	FAPFFAFK	452	1795
HLA-A*68:01	SASFSTFK	FAPFFAFK	1114	290
HLA-B*15:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	164	629
HLA-A*03:02	NSASFSTFK	NFAPFFAFK	37	135
HLA-A*29:02	SVLYNSASF	SVLYNFAPF	1130	313
HLA-A*30:02	NSASFSTFKCY	NFAPFFAFKCY	387	1395
HLA-C*12:03	SASFSTFKCY	FAPFFAFKCY	498	139
HLA-C*14:02	LYNSASFSTF	LYNFAPFFAF	20	69
HLA-A*30:02	VLYNSASFSTFKCY	VLYNFAPFFAFKCY	272	923
HLA-A*32:01	SVLYNSASFSTF	SVLYNFAPFFAF	1624	488
HLA-A*31:01	VLYNSASFSTFK	VLYNFAPFFAFK	565	170
HLA-A*68:01	NSASFSTFK	NFAPFFAFK	4	13
HLA-A*31:01	LYNSASFSTFK	LYNFAPFFAFK	299	92
HLA-A*11:01	YNSASFSTFK	YNFAPFFAFK	12	38
HLA-A*24:02	LYNSASFSTFK	LYNFAPFFAFK	356	1101
HLA-A*26:01	SVLYNSASF	SVLYNFAPF	1313	431
HLA-A*33:03	LYNSASFSTFK	LYNFAPFFAFK	1158	383
HLA-A*24:02	VLYNSASFSTF	VLYNFAPFFAF	186	551
HLA-A*03:01	VLYNSASFSTFKC	VLYNFAPFFAFKC	312	920
HLA-A*02:06	SASFSTFKCYGV	FAPFFAFKCYGV	1416	481
HLA-C*03:02	YSVLYNSASFSTF	YSVLYNFAPFFAF	113	329
HLA-A*23:01	YSVLYNSASF	YSVLYNFAPF	1120	403
HLA-B*58:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	429	1160
HLA-A*29:02	LYNSASFSTFKCY	LYNFAPFFAFKCY	297	115
HLA-C*12:02	SASFSTFKCY	FAPFFAFKCY	710	275
HLA-B*15:03	VLYNSASFSTF	VLYNFAPFFAF	38	96
HLA-A*68:01	NSASFSTFKC	NFAPFFAFKC	281	702
HLA-A*03:01	VLYNSASFSTFK	VLYNFAPFFAFK	7	17
HLA-A*03:01	YNSASFSTFK	YNFAPFFAFK	80	194
HLA-A*11:01	VLYNSASFSTFK	VLYNFAPFFAFK	22	51
HLA-B*15:01	SVLYNSASFSTF	SVLYNFAPFFAF	221	509
HLA-B*15:01	LYNSASFSTF	LYNFAPFFAF	468	1064
HLA-B*15:01	SASFSTFKCY	FAPFFAFKCY	256	561
HLA-C*03:02	SASFSTFKCY	FAPFFAFKCY	212	97
HLA-A*23:01	DYSVLYNSASF	DYSVLYNFAPF	1039	482
HLA-A*68:01	YSVLYNSASFSTFK	YSVLYNFAPFFAFK	144	67

S373P

Reference  ASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYAD
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    ASVYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYAD

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*32:01	VLYNSASFS	VLYNFAPFF	22186	33
HLA-A*23:01	VLYNSASFS	VLYNFAPFF	32767	84
HLA-B*15:03	VLYNSASFS	VLYNFAPFF	3280	11
HLA-A*29:02	VLYNSASFS	VLYNFAPFF	10439	66
HLA-A*23:01	LYNSASFS	LYNFAPFF	22508	183
HLA-A*24:02	LYNSASFS	LYNFAPFF	25164	266
HLA-A*32:01	SVLYNSASFS	SVLYNFAPFF	14340	178
HLA-A*24:02	VLYNSASFS	VLYNFAPFF	32767	429
HLA-A*29:02	SVLYNSASFS	SVLYNFAPFF	16185	256
HLA-A*30:02	ASFSTFKCY	APFFAFKCY	43	2647
HLA-B*15:01	VLYNSASFS	VLYNFAPFF	8189	139
HLA-C*03:02	VLYNSASFS	VLYNFAPFF	13004	222
HLA-B*15:01	ASFSTFKCY	APFFAFKCY	107	6085
HLA-A*23:01	SVLYNSASFS	SVLYNFAPFF	24524	482
HLA-A*02:06	ASFSTFKCYGV	APFFAFKCYGV	231	11537
HLA-C*14:02	LYNSASFS	LYNFAPFF	5693	117
HLA-C*16:01	ASFSTFKCY	APFFAFKCY	68	3117
HLA-C*12:03	ASFSTFKCY	APFFAFKCY	115	5066
HLA-C*03:02	ASFSTFKCY	APFFAFKCY	64	2656
HLA-B*15:03	SVLYNSASFS	SVLYNFAPFF	2850	70
HLA-A*01:01	NSASFSTFKCY	NFAPFFAFKCY	328	13051
HLA-A*23:01	DYSVLYNSASFS	DYSVLYNFAPFF	14260	374
HLA-C*16:01	NSASFSTF	NFAPFFAF	243	8562
HLA-A*11:01	ASFSTFKCY	APFFAFKCY	379	12606
HLA-A*02:11	SVLYNSASFST	SVLYNFAPFFA	3830	120
HLA-C*14:02	VLYNSASFS	VLYNFAPFF	11085	354
HLA-A*02:01	VLYNSASFST	VLYNFAPFFA	243	8
HLA-A*23:01	NSASFSTF	NFAPFFAF	12240	424
HLA-A*29:02	YNSASFSTF	YNFAPFFAF	1671	60
HLA-B*35:01	ASFSTFKCY	APFFAFKCY	2345	92
HLA-C*12:02	ASFSTFKCY	APFFAFKCY	338	8222
HLA-B*58:01	YSVLYNSASFS	YSVLYNFAPFF	6920	296
HLA-A*68:02	NSASFSTFKCYGV	NFAPFFAFKCYGV	48	1043
HLA-C*14:02	NSASFSTF	NFAPFFAF	2544	120
HLA-B*15:03	ASFSTFKCY	APFFAFKCY	25	529
HLA-B*15:01	SVLYNSASFS	SVLYNFAPFF	2838	139
HLA-A*02:02	VLYNSASFST	VLYNFAPFFA	202	10
HLA-C*03:02	SVLYNSASFS	SVLYNFAPFF	8888	443
HLA-A*02:06	VLYNSASFST	VLYNFAPFFA	710	36
HLA-B*15:03	YSVLYNSASFS	YSVLYNFAPFF	3170	164
HLA-A*02:11	VLYNSASFST	VLYNFAPFFA	77	4
HLA-A*02:01	SVLYNSASFST	SVLYNFAPFFA	5674	301
HLA-A*11:01	LYNSASFSTFK	LYNFAPFFAFK	43	797
HLA-A*30:02	SASFSTFKCY	FAPFFAFKCY	71	1241
HLA-A*02:11	YSVLYNSASFST	YSVLYNFAPFFA	7859	469
HLA-C*14:02	SFSTFKCY	PFFAFKCY	386	6449
HLA-A*32:01	YNSASFSTF	YNFAPFFAF	4333	294
HLA-A*33:03	YNSASFSTFK	YNFAPFFAFK	823	57
HLA-A*02:06	SVLYNSASFST	SVLYNFAPFFA	4097	319
HLA-B*18:01	YNSASFSTF	YNFAPFFAF	3495	284
HLA-A*33:03	NSASFSTFK	NFAPFFAFK	181	16
HLA-A*02:11	VLYNSASFSTF	VLYNFAPFFAF	2537	236
HLA-A*11:01	NSASFSTFK	NFAPFFAFK	9	94
HLA-A*29:02	NSASFSTFKCY	NFAPFFAFKCY	662	64
HLA-A*29:02	YNSASFSTFKCY	YNFAPFFAFKCY	842	86
HLA-A*02:01	VLYNSASFSTF	VLYNFAPFFAF	4199	455
HLA-C*03:02	YSVLYNSASFS	YSVLYNFAPFF	2405	264
HLA-A*29:02	ASFSTFKCY	APFFAFKCY	201	1830
HLA-A*02:06	SVLYNSASF	SVLYNFAPF	2112	234
HLA-B*15:01	VLYNSASFSTF	VLYNFAPFFAF	50	420
HLA-A*31:01	YNSASFSTFK	YNFAPFFAFK	607	73
HLA-A*30:01	LYNSASFST	LYNFAPFFA	3467	433
HLA-A*33:03	SVLYNSASFSTFK	SVLYNFAPFFAFK	2778	357
HLA-B*35:01	SASFSTFKCY	FAPFFAFKCY	490	65
HLA-A*31:01	NSASFSTFK	NFAPFFAFK	293	47
HLA-A*29:02	LYNSASFSTF	LYNFAPFFAF	387	63
HLA-A*30:02	VLYNSASFS	VLYNFAPFF	2919	476
HLA-A*31:01	SVLYNSASFSTFK	SVLYNFAPFFAFK	857	148
HLA-A*23:01	YNSASFSTF	YNFAPFFAF	352	63
HLA-A*03:01	LYNSASFSTFK	LYNFAPFFAFK	295	1614
HLA-A*23:01	SVLYNSASF	SVLYNFAPF	990	197
HLA-A*02:11	SVLYNSASFS	SVLYNFAPFF	2012	435
HLA-A*03:01	NSASFSTFK	NFAPFFAFK	113	494
HLA-B*35:01	YNSASFSTF	YNFAPFFAF	760	180
HLA-A*03:02	LYNSASFSTFK	LYNFAPFFAFK	202	831
HLA-A*02:01	VLYNSASFS	VLYNFAPFF	1548	377
HLA-A*02:11	VLYNSASFS	VLYNFAPFF	258	63
HLA-A*01:01	SASFSTFKCY	FAPFFAFKCY	461	1849
HLA-A*11:01	SASFSTFK	FAPFFAFK	452	1795
HLA-A*68:01	SASFSTFK	FAPFFAFK	1114	290
HLA-B*15:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	164	629
HLA-A*03:02	NSASFSTFK	NFAPFFAFK	37	135
HLA-A*29:02	SVLYNSASF	SVLYNFAPF	1130	313
HLA-A*30:02	NSASFSTFKCY	NFAPFFAFKCY	387	1395
HLA-C*12:03	SASFSTFKCY	FAPFFAFKCY	498	139
HLA-C*14:02	LYNSASFSTF	LYNFAPFFAF	20	69
HLA-A*30:02	VLYNSASFSTFKCY	VLYNFAPFFAFKCY	272	923
HLA-A*32:01	SVLYNSASFSTF	SVLYNFAPFFAF	1624	488
HLA-A*31:01	VLYNSASFSTFK	VLYNFAPFFAFK	565	170
HLA-A*68:01	NSASFSTFK	NFAPFFAFK	4	13
HLA-A*31:01	LYNSASFSTFK	LYNFAPFFAFK	299	92
HLA-A*11:01	YNSASFSTFK	YNFAPFFAFK	12	38
HLA-A*24:02	LYNSASFSTFK	LYNFAPFFAFK	356	1101
HLA-A*26:01	SVLYNSASF	SVLYNFAPF	1313	431
HLA-A*33:03	LYNSASFSTFK	LYNFAPFFAFK	1158	383
HLA-A*24:02	VLYNSASFSTF	VLYNFAPFFAF	186	551
HLA-A*03:01	VLYNSASFSTFKC	VLYNFAPFFAFKC	312	920
HLA-A*02:06	SASFSTFKCYGV	FAPFFAFKCYGV	1416	481
HLA-C*03:02	YSVLYNSASFSTF	YSVLYNFAPFFAF	113	329
HLA-A*23:01	YSVLYNSASF	YSVLYNFAPF	1120	403
HLA-B*58:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	429	1160
HLA-A*29:02	LYNSASFSTFKCY	LYNFAPFFAFKCY	297	115
HLA-C*12:02	SASFSTFKCY	FAPFFAFKCY	710	275
HLA-B*15:03	VLYNSASFSTF	VLYNFAPFFAF	38	96
HLA-A*68:01	NSASFSTFKC	NFAPFFAFKC	281	702
HLA-A*03:01	VLYNSASFSTFK	VLYNFAPFFAFK	7	17
HLA-A*03:01	YNSASFSTFK	YNFAPFFAFK	80	194
HLA-A*11:01	VLYNSASFSTFK	VLYNFAPFFAFK	22	51
HLA-B*15:01	SVLYNSASFSTF	SVLYNFAPFFAF	221	509
HLA-B*15:01	LYNSASFSTF	LYNFAPFFAF	468	1064
HLA-B*15:01	SASFSTFKCY	FAPFFAFKCY	256	561
HLA-C*03:02	SASFSTFKCY	FAPFFAFKCY	212	97
HLA-A*23:01	DYSVLYNSASF	DYSVLYNFAPF	1039	482
HLA-A*68:01	YSVLYNSASFSTFK	YSVLYNFAPFFAFK	144	67

375-376 ST->FA

Reference  VYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFV
           |||||||||||||||||||||||||  |||||||||||||||||||||||||
Mutated    VYAWNRKRISNCVADYSVLYNFAPFFAFKCYGVSPTKLNDLCFTNVYADSFV

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*32:01	VLYNSASFS	VLYNFAPFF	22186	33
HLA-A*23:01	VLYNSASFS	VLYNFAPFF	32767	84
HLA-B*15:03	VLYNSASFS	VLYNFAPFF	3280	11
HLA-A*29:02	VLYNSASFS	VLYNFAPFF	10439	66
HLA-A*23:01	LYNSASFS	LYNFAPFF	22508	183
HLA-A*24:02	LYNSASFS	LYNFAPFF	25164	266
HLA-A*32:01	SVLYNSASFS	SVLYNFAPFF	14340	178
HLA-A*24:02	VLYNSASFS	VLYNFAPFF	32767	429
HLA-A*29:02	SVLYNSASFS	SVLYNFAPFF	16185	256
HLA-A*30:02	ASFSTFKCY	APFFAFKCY	43	2647
HLA-B*15:01	VLYNSASFS	VLYNFAPFF	8189	139
HLA-C*03:02	VLYNSASFS	VLYNFAPFF	13004	222
HLA-B*15:01	ASFSTFKCY	APFFAFKCY	107	6085
HLA-A*23:01	SVLYNSASFS	SVLYNFAPFF	24524	482
HLA-A*02:06	ASFSTFKCYGV	APFFAFKCYGV	231	11537
HLA-C*14:02	LYNSASFS	LYNFAPFF	5693	117
HLA-C*15:02	FSTFKCYGV	FFAFKCYGV	431	20837
HLA-C*16:01	ASFSTFKCY	APFFAFKCY	68	3117
HLA-C*12:03	ASFSTFKCY	APFFAFKCY	115	5066
HLA-C*03:02	ASFSTFKCY	APFFAFKCY	64	2656
HLA-A*11:01	STFKCYGVSPTK	FAFKCYGVSPTK	25	1030
HLA-B*15:03	SVLYNSASFS	SVLYNFAPFF	2850	70
HLA-A*01:01	NSASFSTFKCY	NFAPFFAFKCY	328	13051
HLA-A*23:01	DYSVLYNSASFS	DYSVLYNFAPFF	14260	374
HLA-C*16:01	NSASFSTF	NFAPFFAF	243	8562
HLA-A*11:01	ASFSTFKCY	APFFAFKCY	379	12606
HLA-A*02:11	SVLYNSASFST	SVLYNFAPFFA	3830	120
HLA-C*14:02	VLYNSASFS	VLYNFAPFF	11085	354
HLA-A*02:01	VLYNSASFST	VLYNFAPFFA	243	8
HLA-A*23:01	NSASFSTF	NFAPFFAF	12240	424
HLA-A*29:02	YNSASFSTF	YNFAPFFAF	1671	60
HLA-C*16:01	FSTFKCYGV	FFAFKCYGV	302	8277
HLA-B*35:01	ASFSTFKCY	APFFAFKCY	2345	92
HLA-C*12:02	ASFSTFKCY	APFFAFKCY	338	8222
HLA-B*58:01	YSVLYNSASFS	YSVLYNFAPFF	6920	296
HLA-A*03:01	STFKCYGVSPTK	FAFKCYGVSPTK	83	1880
HLA-A*68:02	NSASFSTFKCYGV	NFAPFFAFKCYGV	48	1043
HLA-C*14:02	NSASFSTF	NFAPFFAF	2544	120
HLA-B*15:03	ASFSTFKCY	APFFAFKCY	25	529
HLA-B*15:01	SVLYNSASFS	SVLYNFAPFF	2838	139
HLA-A*02:02	VLYNSASFST	VLYNFAPFFA	202	10
HLA-C*03:02	SVLYNSASFS	SVLYNFAPFF	8888	443
HLA-C*03:02	STFKCYGVS	FAFKCYGVS	8370	424
HLA-A*02:06	VLYNSASFST	VLYNFAPFFA	710	36
HLA-B*15:03	YSVLYNSASFS	YSVLYNFAPFF	3170	164
HLA-A*02:11	VLYNSASFST	VLYNFAPFFA	77	4
HLA-A*02:01	SVLYNSASFST	SVLYNFAPFFA	5674	301
HLA-A*11:01	LYNSASFSTFK	LYNFAPFFAFK	43	797
HLA-A*03:02	STFKCYGVSPTK	FAFKCYGVSPTK	142	2541
HLA-A*30:02	SASFSTFKCY	FAPFFAFKCY	71	1241
HLA-A*02:11	YSVLYNSASFST	YSVLYNFAPFFA	7859	469
HLA-C*14:02	SFSTFKCY	PFFAFKCY	386	6449
HLA-A*30:01	STFKCYGVS	FAFKCYGVS	438	6895
HLA-A*68:02	FSTFKCYGV	FFAFKCYGV	17	261
HLA-A*32:01	YNSASFSTF	YNFAPFFAF	4333	294
HLA-A*02:06	FSTFKCYGV	FFAFKCYGV	61	882
HLA-A*33:03	YNSASFSTFK	YNFAPFFAFK	823	57
HLA-A*11:01	FSTFKCYGVSPTK	FFAFKCYGVSPTK	426	5482
HLA-A*02:06	SVLYNSASFST	SVLYNFAPFFA	4097	319
HLA-B*18:01	YNSASFSTF	YNFAPFFAF	3495	284
HLA-A*33:03	NSASFSTFK	NFAPFFAFK	181	16
HLA-C*14:02	FSTFKCYGV	FFAFKCYGV	4043	371
HLA-A*02:11	VLYNSASFSTF	VLYNFAPFFAF	2537	236
HLA-A*11:01	NSASFSTFK	NFAPFFAFK	9	94
HLA-A*29:02	NSASFSTFKCY	NFAPFFAFKCY	662	64
HLA-A*29:02	YNSASFSTFKCY	YNFAPFFAFKCY	842	86
HLA-A*02:01	VLYNSASFSTF	VLYNFAPFFAF	4199	455
HLA-C*03:02	YSVLYNSASFS	YSVLYNFAPFF	2405	264
HLA-A*29:02	ASFSTFKCY	APFFAFKCY	201	1830
HLA-B*15:01	VLYNSASFSTF	VLYNFAPFFAF	50	420
HLA-A*31:01	YNSASFSTFK	YNFAPFFAFK	607	73
HLA-A*30:01	LYNSASFST	LYNFAPFFA	3467	433
HLA-A*33:03	SVLYNSASFSTFK	SVLYNFAPFFAFK	2778	357
HLA-B*35:01	SASFSTFKCY	FAPFFAFKCY	490	65
HLA-A*30:01	STFKCYGVSPTK	FAFKCYGVSPTK	194	1428
HLA-A*31:01	NSASFSTFK	NFAPFFAFK	293	47
HLA-A*29:02	LYNSASFSTF	LYNFAPFFAF	387	63
HLA-A*30:02	VLYNSASFS	VLYNFAPFF	2919	476
HLA-A*31:01	SVLYNSASFSTFK	SVLYNFAPFFAFK	857	148
HLA-A*23:01	YNSASFSTF	YNFAPFFAF	352	63
HLA-A*03:01	LYNSASFSTFK	LYNFAPFFAFK	295	1614
HLA-A*02:11	SVLYNSASFS	SVLYNFAPFF	2012	435
HLA-A*03:01	NSASFSTFK	NFAPFFAFK	113	494
HLA-B*35:01	YNSASFSTF	YNFAPFFAF	760	180
HLA-A*03:02	LYNSASFSTFK	LYNFAPFFAFK	202	831
HLA-A*02:01	VLYNSASFS	VLYNFAPFF	1548	377
HLA-A*02:11	VLYNSASFS	VLYNFAPFF	258	63
HLA-A*01:01	SASFSTFKCY	FAPFFAFKCY	461	1849
HLA-A*11:01	SASFSTFK	FAPFFAFK	452	1795
HLA-A*68:01	SASFSTFK	FAPFFAFK	1114	290
HLA-B*15:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	164	629
HLA-A*03:02	NSASFSTFK	NFAPFFAFK	37	135
HLA-A*30:02	NSASFSTFKCY	NFAPFFAFKCY	387	1395
HLA-C*12:03	SASFSTFKCY	FAPFFAFKCY	498	139
HLA-C*14:02	LYNSASFSTF	LYNFAPFFAF	20	69
HLA-A*30:02	VLYNSASFSTFKCY	VLYNFAPFFAFKCY	272	923
HLA-A*32:01	SVLYNSASFSTF	SVLYNFAPFFAF	1624	488
HLA-A*31:01	VLYNSASFSTFK	VLYNFAPFFAFK	565	170
HLA-A*68:01	NSASFSTFK	NFAPFFAFK	4	13
HLA-A*31:01	LYNSASFSTFK	LYNFAPFFAFK	299	92
HLA-A*11:01	YNSASFSTFK	YNFAPFFAFK	12	38
HLA-A*24:02	LYNSASFSTFK	LYNFAPFFAFK	356	1101
HLA-A*33:03	LYNSASFSTFK	LYNFAPFFAFK	1158	383
HLA-A*24:02	VLYNSASFSTF	VLYNFAPFFAF	186	551
HLA-A*03:01	VLYNSASFSTFKC	VLYNFAPFFAFKC	312	920
HLA-A*02:06	SASFSTFKCYGV	FAPFFAFKCYGV	1416	481
HLA-C*03:02	YSVLYNSASFSTF	YSVLYNFAPFFAF	113	329
HLA-A*68:01	FSTFKCYGVSPTK	FFAFKCYGVSPTK	288	832
HLA-B*58:01	YSVLYNSASFSTF	YSVLYNFAPFFAF	429	1160
HLA-A*29:02	LYNSASFSTFKCY	LYNFAPFFAFKCY	297	115
HLA-C*12:02	SASFSTFKCY	FAPFFAFKCY	710	275
HLA-B*15:03	VLYNSASFSTF	VLYNFAPFFAF	38	96
HLA-A*68:01	NSASFSTFKC	NFAPFFAFKC	281	702
HLA-A*03:01	VLYNSASFSTFK	VLYNFAPFFAFK	7	17
HLA-A*03:01	YNSASFSTFK	YNFAPFFAFK	80	194
HLA-A*11:01	VLYNSASFSTFK	VLYNFAPFFAFK	22	51
HLA-B*15:01	SVLYNSASFSTF	SVLYNFAPFFAF	221	509
HLA-B*15:01	LYNSASFSTF	LYNFAPFFAF	468	1064
HLA-B*15:01	SASFSTFKCY	FAPFFAFKCY	256	561
HLA-C*03:02	SASFSTFKCY	FAPFFAFKCY	212	97
HLA-A*68:01	YSVLYNSASFSTFK	YSVLYNFAPFFAFK	144	67
HLA-A*30:01	TFKCYGVSPTK	AFKCYGVSPTK	319	155
HLA-A*02:01	FSTFKCYGV	FFAFKCYGV	457	926

D405N

Reference  YGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFT
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    YGVSPTKLNDLCFTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFT

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:01	FVIRGDEVR	FVIRGNEVS	23	15265
HLA-A*68:01	SFVIRGDEVR	SFVIRGNEVS	186	29639
HLA-A*68:01	DSFVIRGDEVR	DSFVIRGNEVS	220	29286
HLA-A*33:03	FVIRGDEVR	FVIRGNEVS	320	29448
HLA-A*33:03	SFVIRGDEVR	SFVIRGNEVS	459	31986
HLA-C*14:02	SFVIRGDEV	SFVIRGNEV	216	46
HLA-C*06:02	IRGDEVRQI	IRGNEVSQI	147	642

R408S

Reference  SPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCV
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    SPTKLNDLCFTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCV

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:01	FVIRGDEVR	FVIRGNEVS	23	15265
HLA-A*03:01	RQIAPGQTGK	SQIAPGQTGN	49	32429
HLA-A*11:01	RQIAPGQTGK	SQIAPGQTGN	103	32387
HLA-A*03:02	RQIAPGQTGK	SQIAPGQTGN	123	32767
HLA-A*68:01	EVRQIAPGQTGK	EVSQIAPGQTGN	144	32361
HLA-A*68:01	SFVIRGDEVR	SFVIRGNEVS	186	29639
HLA-A*68:01	DSFVIRGDEVR	DSFVIRGNEVS	220	29286
HLA-A*33:03	FVIRGDEVR	FVIRGNEVS	320	29448
HLA-A*33:03	SFVIRGDEVR	SFVIRGNEVS	459	31986
HLA-A*30:01	RQIAPGQTGK	SQIAPGQTGN	275	18394
HLA-C*06:02	IRGDEVRQI	IRGNEVSQI	147	642
HLA-B*15:03	RQIAPGQT	SQIAPGQT	478	1764
HLA-B*15:03	RQIAPGQTGKIADY	SQIAPGQTGNIADY	17	55
HLA-A*30:02	RQIAPGQTGKIADY	SQIAPGQTGNIADY	324	951
HLA-B*15:03	RQIAPGQTGKI	SQIAPGQTGNI	236	691
HLA-B*15:03	RQIAPGQTG	SQIAPGQTG	39	107

K417N

Reference  FTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLD
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    FTNVYADSFVIRGNEVSQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNKLD

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*03:01	RQIAPGQTGK	SQIAPGQTGN	49	32429
HLA-A*11:01	RQIAPGQTGK	SQIAPGQTGN	103	32387
HLA-A*03:02	RQIAPGQTGK	SQIAPGQTGN	123	32767
HLA-A*68:01	EVRQIAPGQTGK	EVSQIAPGQTGN	144	32361
HLA-A*68:01	QIAPGQTGK	QIAPGQTGN	136	27997
HLA-A*11:01	QIAPGQTGK	QIAPGQTGN	165	32767
HLA-A*03:02	QIAPGQTGK	QIAPGQTGN	278	32767
HLA-A*03:01	QIAPGQTGK	QIAPGQTGN	296	32767
HLA-A*32:01	KIADYNYKL	NIADYNYKL	58	4480
HLA-A*30:01	RQIAPGQTGK	SQIAPGQTGN	275	18394
HLA-A*68:02	KIADYNYKL	NIADYNYKL	1274	39
HLA-A*03:02	KIADYNYK	NIADYNYK	476	6850
HLA-A*02:06	KIADYNYKL	NIADYNYKL	20	101
HLA-A*02:01	KIADYNYKL	NIADYNYKL	23	110
HLA-C*17:01	KIADYNYKL	NIADYNYKL	155	669
HLA-A*30:01	TGKIADYNYK	TGNIADYNYK	292	1164
HLA-A*02:11	KIADYNYKL	NIADYNYKL	5	19
HLA-B*15:03	RQIAPGQTGKIADY	SQIAPGQTGNIADY	17	55
HLA-A*30:02	RQIAPGQTGKIADY	SQIAPGQTGNIADY	324	951
HLA-B*15:03	RQIAPGQTGKI	SQIAPGQTGNI	236	691
HLA-A*68:01	TGKIADYNYK	TGNIADYNYK	957	343
HLA-A*01:01	QTGKIADYNY	QTGNIADYNY	580	270

N440K

Reference  TGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFE
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    TGNIADYNYKLPDDFTGCVIAWNSNKLDSKVGGNYNYRYRLFRKSNLKPFE

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*11:01	CVIAWNSNN	CVIAWNSNK	24486	26
HLA-A*68:01	CVIAWNSNN	CVIAWNSNK	11802	16
HLA-A*68:01	FTGCVIAWNSNN	FTGCVIAWNSNK	25655	59
HLA-A*03:01	CVIAWNSNN	CVIAWNSNK	24643	61
HLA-A*03:02	CVIAWNSNN	CVIAWNSNK	22621	61
HLA-A*11:01	GCVIAWNSNN	GCVIAWNSNK	32767	94
HLA-A*03:01	GCVIAWNSNN	GCVIAWNSNK	32767	128
HLA-A*11:01	TGCVIAWNSNN	TGCVIAWNSNK	32767	153
HLA-A*68:01	TGCVIAWNSNN	TGCVIAWNSNK	32767	180
HLA-A*68:01	DFTGCVIAWNSNN	DFTGCVIAWNSNK	32767	255
HLA-A*03:02	GCVIAWNSNN	GCVIAWNSNK	32767	277
HLA-A*31:01	NLDSKVGGNYNYL	KLDSKVGGNYNYR	31768	301
HLA-A*11:01	FTGCVIAWNSNN	FTGCVIAWNSNK	32767	408
HLA-A*31:01	CVIAWNSNN	CVIAWNSNK	18631	279
HLA-A*33:03	CVIAWNSNN	CVIAWNSNK	20952	493
HLA-A*30:01	CVIAWNSNN	CVIAWNSNK	11396	467
HLA-A*01:01	NLDSKVGGNYNYLY	KLDSKVGGNYNYRY	193	2088
HLA-A*30:02	NLDSKVGGNYNY	KLDSKVGGNYNY	3839	409
HLA-A*30:02	NLDSKVGGNY	KLDSKVGGNY	422	52
HLA-B*15:01	NLDSKVGGNY	KLDSKVGGNY	1404	179
HLA-A*01:01	NLDSKVGGNY	KLDSKVGGNY	371	819
HLA-A*02:11	VIAWNSNNL	VIAWNSNKL	269	586
HLA-A*68:02	FTGCVIAWNSNNL	FTGCVIAWNSNKL	461	960

L452R

Reference  DDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGS
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    DDFTGCVIAWNSNKLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGN

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*31:01	KVGGNYNYL	KVGGNYNYR	5813	13
HLA-A*02:11	YLYRLFRKSNL	YRYRLFRKSNL	82	22451
HLA-A*68:01	SKVGGNYNYL	SKVGGNYNYR	24692	119
HLA-B*27:05	YLYRLFRK	YRYRLFRK	9806	50
HLA-A*02:11	YLYRLFRKS	YRYRLFRKS	179	28031
HLA-A*02:02	YLYRLFRKSNL	YRYRLFRKSNL	188	23354
HLA-A*02:11	KVGGNYNYL	KVGGNYNYR	111	13729
HLA-B*27:05	YLYRLFRKS	YRYRLFRKS	13862	117
HLA-A*31:01	NLDSKVGGNYNYL	KLDSKVGGNYNYR	31768	301
HLA-A*33:03	DSKVGGNYNYL	DSKVGGNYNYR	30679	292
HLA-B*27:05	YLYRLFRKSNLK	YRYRLFRKSNLK	2218	22
HLA-A*31:01	SKVGGNYNYL	SKVGGNYNYR	21677	233
HLA-A*03:01	YLYRLFRK	YRYRLFRK	204	18425
HLA-A*68:01	KVGGNYNYL	KVGGNYNYR	25432	286
HLA-A*33:03	KVGGNYNYL	KVGGNYNYR	22954	269
HLA-A*11:01	KVGGNYNYL	KVGGNYNYR	16150	194
HLA-A*02:02	KVGGNYNYL	KVGGNYNYR	279	23086
HLA-A*68:01	DSKVGGNYNYL	DSKVGGNYNYR	28915	378
HLA-A*03:02	YLYRLFRK	YRYRLFRK	327	22983
HLA-A*02:01	YLYRLFRKSNL	YRYRLFRKSNL	471	28211
HLA-A*33:03	SKVGGNYNYL	SKVGGNYNYR	27973	469
HLA-A*03:02	KVGGNYNYL	KVGGNYNYR	12848	234
HLA-A*02:06	KVGGNYNYL	KVGGNYNYR	452	18600
HLA-B*27:05	YLYRLFRKSNLKP	YRYRLFRKSNLKP	13004	347
HLA-B*27:05	YLYRLFRKSNLKPF	YRYRLFRKSNLKPF	7101	214
HLA-B*27:05	YLYRLFRKSNL	YRYRLFRKSNL	1360	48
HLA-B*15:01	YLYRLFRKSNLKPF	YRYRLFRKSNLKPF	476	9205
HLA-B*08:01	YLYRLFRKSNL	YRYRLFRKSNL	160	1946
HLA-A*30:01	LYRLFRKSN	RYRLFRKSN	1444	120
HLA-A*30:01	LYRLFRKSNL	RYRLFRKSNL	1104	93
HLA-A*02:11	KVGGNYNYLYRL	KVGGNYNYRYRL	274	3087
HLA-A*30:01	LYRLFRKSNLKP	RYRLFRKSNLKP	3059	277
HLA-A*01:01	NLDSKVGGNYNYLY	KLDSKVGGNYNYRY	193	2088
HLA-A*30:01	LYRLFRKSNLK	RYRLFRKSNLK	192	21
HLA-A*03:01	LYRLFRKSNLKP	RYRLFRKSNLKP	3564	430
HLA-A*30:01	NYLYRLFRK	NYRYRLFRK	512	64
HLA-B*27:05	LYRLFRKSNL	RYRLFRKSNL	932	123
HLA-A*23:01	GNYNYLYRLF	GNYNYRYRLF	181	1119
HLA-A*23:01	NYNYLYRLF	NYNYRYRLF	20	119
HLA-A*23:01	KVGGNYNYLYRLF	KVGGNYNYRYRLF	410	2404
HLA-A*23:01	VGGNYNYLYRLF	VGGNYNYRYRLF	222	1236
HLA-A*29:02	VGGNYNYLY	VGGNYNYRY	94	518
HLA-A*31:01	LYRLFRKSNLK	RYRLFRKSNLK	356	67
HLA-A*30:01	YLYRLFRKSNLK	YRYRLFRKSNLK	1354	258
HLA-A*24:02	NYNYLYRLF	NYNYRYRLF	21	108
HLA-A*29:02	KVGGNYNYLY	KVGGNYNYRY	100	505
HLA-A*24:02	GNYNYLYRLF	GNYNYRYRLF	325	1597
HLA-A*24:02	VGGNYNYLYRLF	VGGNYNYRYRLF	391	1596
HLA-A*03:01	LYRLFRKSNLK	RYRLFRKSNLK	65	18
HLA-A*03:02	LYRLFRKSNLK	RYRLFRKSNLK	242	72
HLA-A*30:01	YNYLYRLFRK	YNYRYRLFRK	379	125
HLA-A*11:01	LYRLFRKSNLK	RYRLFRKSNLK	952	401
HLA-B*15:03	SKVGGNYNYLY	SKVGGNYNYRY	313	720
HLA-A*30:02	VGGNYNYLY	VGGNYNYRY	45	93

477-478 ST->NK

Reference  LYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGV
           |||||||||||||||||||||||||  |||||||||||||||||||||||||
Mutated    RYRLFRKSNLKPFERDISTEIYQAGNKPCNGVAGVNCYFPLQSYGFRPTYGV

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*11:01	STEIYQAGST	STEIYQAGNK	30358	67
HLA-A*68:01	STEIYQAGST	STEIYQAGNK	22142	157
HLA-A*02:02	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	9447	68
HLA-A*03:01	STEIYQAGST	STEIYQAGNK	32241	308
HLA-A*02:11	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	17568	184
HLA-B*35:01	TPCNGVEGF	KPCNGVAGV	319	21824
HLA-A*02:06	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	5118	87
HLA-B*15:03	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	217	12581
HLA-B*15:01	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	408	12442
HLA-A*02:11	YQAGSTPCNGV	YQAGNKPCNGV	173	678
HLA-B*50:01	TEIYQAGSTP	TEIYQAGNKP	464	1807
HLA-A*02:06	YQAGSTPCNGV	YQAGNKPCNGV	39	146
HLA-A*02:01	YQAGSTPCNGV	YQAGNKPCNGV	420	1490
HLA-C*14:02	IYQAGSTPC	IYQAGNKPC	134	459
HLA-A*02:02	YQAGSTPCNGV	YQAGNKPCNGV	107	233

E484A

Reference  SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    SNLKPFERDISTEIYQAGNKPCNGVAGVNCYFPLQSYGFRPTYGVGHQPYR

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*40:02	VEGFNCYFPL	VAGVNCYFPL	31	19248
HLA-B*40:01	VEGFNCYFPL	VAGVNCYFPL	76	26041
HLA-A*02:02	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	9447	68
HLA-A*02:11	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	17568	184
HLA-B*35:01	TPCNGVEGF	KPCNGVAGV	319	21824
HLA-A*02:06	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	5118	87
HLA-B*15:03	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	217	12581
HLA-A*68:02	EGFNCYFPL	AGVNCYFPL	117	4903
HLA-B*15:01	GVEGFNCYF	GVAGVNCYF	3125	98
HLA-B*15:01	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	408	12442
HLA-B*15:03	GVEGFNCYF	GVAGVNCYF	1657	235
HLA-A*30:02	EGFNCYFPLQSY	AGVNCYFPLQSY	1806	447
HLA-A*02:02	GVEGFNCYFPL	GVAGVNCYFPL	666	171
HLA-B*35:01	NGVEGFNCY	NGVAGVNCY	346	866

F486V

Reference  LKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVV
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    LKPFERDISTEIYQAGNKPCNGVAGVNCYFPLQSYGFRPTYGVGHQPYRVV

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*40:02	VEGFNCYFPL	VAGVNCYFPL	31	19248
HLA-B*40:01	VEGFNCYFPL	VAGVNCYFPL	76	26041
HLA-A*02:02	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	9447	68
HLA-A*02:11	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	17568	184
HLA-B*35:01	TPCNGVEGF	KPCNGVAGV	319	21824
HLA-A*02:06	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	5118	87
HLA-B*15:03	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	217	12581
HLA-A*68:02	EGFNCYFPL	AGVNCYFPL	117	4903
HLA-B*15:01	GVEGFNCYF	GVAGVNCYF	3125	98
HLA-B*15:01	YQAGSTPCNGVEGF	YQAGNKPCNGVAGV	408	12442
HLA-C*03:02	FNCYFPLQSY	VNCYFPLQSY	225	1964
HLA-B*15:03	GVEGFNCYF	GVAGVNCYF	1657	235
HLA-A*30:02	EGFNCYFPLQSY	AGVNCYFPLQSY	1806	447
HLA-A*02:02	GVEGFNCYFPL	GVAGVNCYFPL	666	171
HLA-A*29:02	GFNCYFPLQSY	GVNCYFPLQSY	191	742
HLA-A*29:02	FNCYFPLQSY	VNCYFPLQSY	73	227
HLA-B*35:01	NGVEGFNCY	NGVAGVNCY	346	866
HLA-B*15:03	FNCYFPLQSY	VNCYFPLQSY	101	240

Q498R

Reference  YQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPAT
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    YQAGNKPCNGVAGVNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAPAT

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*03:02	QSYGFQPTN	QSYGFRPTY	12903	18
HLA-B*15:01	LQSYGFQPTN	LQSYGFRPTY	6135	9
HLA-C*16:01	QSYGFQPTN	QSYGFRPTY	10868	16
HLA-A*29:02	QSYGFQPTN	QSYGFRPTY	26152	43
HLA-B*15:03	QSYGFQPTN	QSYGFRPTY	6543	11
HLA-B*15:03	LQSYGFQPTN	LQSYGFRPTY	2045	4
HLA-C*12:03	QSYGFQPTN	QSYGFRPTY	12696	25
HLA-A*29:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	19514	47
HLA-B*15:03	PLQSYGFQPTN	PLQSYGFRPTY	22802	60
HLA-B*35:01	FPLQSYGFQPTN	FPLQSYGFRPTY	8715	27
HLA-A*33:03	YFPLQSYGFQ	YFPLQSYGFR	11148	35
HLA-C*14:02	SYGFQPTN	SYGFRPTY	20230	66
HLA-A*31:01	CYFPLQSYGFQ	CYFPLQSYGFR	8535	34
HLA-C*03:02	FPLQSYGFQPTN	FPLQSYGFRPTY	23840	111
HLA-C*14:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	18230	85
HLA-A*33:03	CYFPLQSYGFQ	CYFPLQSYGFR	11311	54
HLA-C*03:02	LQSYGFQPTN	LQSYGFRPTY	23421	125
HLA-B*15:01	FQPTNGVGY	FRPTYGVGH	166	29043
HLA-A*30:02	QSYGFQPTN	QSYGFRPTY	5297	32
HLA-A*33:03	FPLQSYGFQ	FPLQSYGFR	23527	146
HLA-B*15:03	FPLQSYGFQPTN	FPLQSYGFRPTY	20159	126
HLA-A*31:01	YFPLQSYGFQ	YFPLQSYGFR	16290	103
HLA-B*35:01	QSYGFQPTN	QSYGFRPTY	24615	156
HLA-C*03:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	27639	176
HLA-C*16:01	LQSYGFQPTN	LQSYGFRPTY	27149	187
HLA-A*30:02	LQSYGFQPTN	LQSYGFRPTY	7508	54
HLA-B*15:01	QSYGFQPTN	QSYGFRPTY	21529	157
HLA-C*12:02	QSYGFQPTN	QSYGFRPTY	21029	169
HLA-B*15:03	YFPLQSYGFQPTN	YFPLQSYGFRPTY	22168	189
HLA-C*16:01	FPLQSYGFQPTN	FPLQSYGFRPTY	27733	237
HLA-A*29:02	CYFPLQSYGFQPTN	CYFPLQSYGFRPTY	27182	240
HLA-A*29:02	LQSYGFQPTN	LQSYGFRPTY	28458	265
HLA-B*15:03	FQPTNGVGY	FRPTYGVGH	63	6390
HLA-A*29:02	GFQPTNGVGY	GFRPTYGVGH	188	18753
HLA-C*16:01	YFPLQSYGFQPTN	YFPLQSYGFRPTY	29358	306
HLA-C*12:03	FPLQSYGFQPTN	FPLQSYGFRPTY	27893	295
HLA-C*14:02	QSYGFQPTN	QSYGFRPTY	25941	277
HLA-A*31:01	NCYFPLQSYGFQ	NCYFPLQSYGFR	21321	230
HLA-A*33:03	NCYFPLQSYGFQ	NCYFPLQSYGFR	22134	257
HLA-C*12:03	LQSYGFQPTN	LQSYGFRPTY	26641	325
HLA-B*15:03	LQSYGFQPTNG	LQSYGFRPTYG	6387	82
HLA-A*32:01	QSYGFQPTN	QSYGFRPTY	16619	226
HLA-A*11:01	QSYGFQPTN	QSYGFRPTY	22068	301
HLA-A*68:01	FPLQSYGFQ	FPLQSYGFR	17691	247
HLA-C*02:02	QSYGFQPTN	QSYGFRPTY	26598	412
HLA-C*02:10	QSYGFQPTN	QSYGFRPTY	26598	412
HLA-A*68:01	YFPLQSYGFQ	YFPLQSYGFR	14480	278
HLA-C*14:02	CYFPLQSYGFQPTN	CYFPLQSYGFRPTY	24158	500
HLA-A*30:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	18743	390
HLA-B*15:01	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	180	8335
HLA-B*15:01	FQPTNGVGYQPY	FRPTYGVGHQPY	290	11634
HLA-B*15:03	YGFQPTNGVGY	YGFRPTYGVGH	368	10641
HLA-A*30:02	GFQPTNGVGY	GFRPTYGVGH	169	4730
HLA-A*30:02	QSYGFQPTNGVGY	QSYGFRPTYGVGH	258	7114
HLA-B*15:03	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	113	2917
HLA-B*53:01	FPLQSYGFQPTN	FPLQSYGFRPTY	8451	351
HLA-C*07:02	FQPTNGVGYQPY	FRPTYGVGHQPY	6169	356
HLA-B*57:01	QSYGFQPTN	QSYGFRPTY	4070	293
HLA-A*30:01	QSYGFQPTN	QSYGFRPTY	3720	308
HLA-B*57:01	LQSYGFQPTN	LQSYGFRPTY	3839	375
HLA-C*14:02	FQPTNGVGYQPY	FRPTYGVGHQPY	3107	366
HLA-B*15:03	FQPTNGVGYQPY	FRPTYGVGHQPY	183	1236
HLA-A*31:01	QPTNGVGYQPYR	RPTYGVGHQPYR	3100	478
HLA-B*58:01	QSYGFQPTN	QSYGFRPTY	1532	249
HLA-A*23:01	SYGFQPTNGV	SYGFRPTYGV	2697	483
HLA-A*02:11	YGFQPTNGV	YGFRPTYGV	307	59
HLA-B*15:03	LQSYGFQPTNGV	LQSYGFRPTYGV	1971	442
HLA-A*02:02	YGFQPTNGV	YGFRPTYGV	868	281
HLA-A*31:01	GFQPTNGVGYQPYR	GFRPTYGVGHQPYR	616	201
HLA-A*02:06	YGFQPTNGV	YGFRPTYGV	174	61
HLA-A*68:02	YGFQPTNGV	YGFRPTYGV	73	26
HLA-C*12:03	YGFQPTNGV	YGFRPTYGV	127	355
HLA-A*02:06	QSYGFQPTNGV	QSYGFRPTYGV	1121	425
HLA-A*68:02	QSYGFQPTNGV	QSYGFRPTYGV	122	47
HLA-A*02:06	LQSYGFQPT	LQSYGFRPT	339	871

N501Y

Reference  GSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCG
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    GNKPCNGVAGVNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAPATVCG

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*03:02	QSYGFQPTN	QSYGFRPTY	12903	18
HLA-B*15:01	LQSYGFQPTN	LQSYGFRPTY	6135	9
HLA-C*16:01	QSYGFQPTN	QSYGFRPTY	10868	16
HLA-A*29:02	QSYGFQPTN	QSYGFRPTY	26152	43
HLA-B*15:03	QSYGFQPTN	QSYGFRPTY	6543	11
HLA-B*15:03	LQSYGFQPTN	LQSYGFRPTY	2045	4
HLA-C*12:03	QSYGFQPTN	QSYGFRPTY	12696	25
HLA-A*29:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	19514	47
HLA-B*15:03	PLQSYGFQPTN	PLQSYGFRPTY	22802	60
HLA-C*14:02	TNGVGYQPY	TYGVGHQPY	5468	16
HLA-B*35:01	FPLQSYGFQPTN	FPLQSYGFRPTY	8715	27
HLA-C*14:02	SYGFQPTN	SYGFRPTY	20230	66
HLA-C*03:02	FPLQSYGFQPTN	FPLQSYGFRPTY	23840	111
HLA-C*14:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	18230	85
HLA-C*03:02	LQSYGFQPTN	LQSYGFRPTY	23421	125
HLA-B*15:01	FQPTNGVGY	FRPTYGVGH	166	29043
HLA-A*30:02	QSYGFQPTN	QSYGFRPTY	5297	32
HLA-B*15:03	FPLQSYGFQPTN	FPLQSYGFRPTY	20159	126
HLA-B*35:01	QSYGFQPTN	QSYGFRPTY	24615	156
HLA-C*03:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	27639	176
HLA-C*16:01	LQSYGFQPTN	LQSYGFRPTY	27149	187
HLA-A*30:02	LQSYGFQPTN	LQSYGFRPTY	7508	54
HLA-B*15:01	QSYGFQPTN	QSYGFRPTY	21529	157
HLA-C*12:02	QSYGFQPTN	QSYGFRPTY	21029	169
HLA-B*15:03	YFPLQSYGFQPTN	YFPLQSYGFRPTY	22168	189
HLA-C*16:01	FPLQSYGFQPTN	FPLQSYGFRPTY	27733	237
HLA-A*29:02	CYFPLQSYGFQPTN	CYFPLQSYGFRPTY	27182	240
HLA-A*29:02	LQSYGFQPTN	LQSYGFRPTY	28458	265
HLA-B*15:03	FQPTNGVGY	FRPTYGVGH	63	6390
HLA-A*29:02	GFQPTNGVGY	GFRPTYGVGH	188	18753
HLA-C*16:01	YFPLQSYGFQPTN	YFPLQSYGFRPTY	29358	306
HLA-C*12:03	FPLQSYGFQPTN	FPLQSYGFRPTY	27893	295
HLA-C*14:02	QSYGFQPTN	QSYGFRPTY	25941	277
HLA-C*12:03	LQSYGFQPTN	LQSYGFRPTY	26641	325
HLA-B*15:03	LQSYGFQPTNG	LQSYGFRPTYG	6387	82
HLA-A*32:01	QSYGFQPTN	QSYGFRPTY	16619	226
HLA-A*11:01	QSYGFQPTN	QSYGFRPTY	22068	301
HLA-C*02:02	QSYGFQPTN	QSYGFRPTY	26598	412
HLA-C*02:10	QSYGFQPTN	QSYGFRPTY	26598	412
HLA-C*14:02	CYFPLQSYGFQPTN	CYFPLQSYGFRPTY	24158	500
HLA-A*30:02	YFPLQSYGFQPTN	YFPLQSYGFRPTY	18743	390
HLA-B*15:01	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	180	8335
HLA-B*15:01	FQPTNGVGYQPY	FRPTYGVGHQPY	290	11634
HLA-C*03:02	NGVGYQPY	YGVGHQPY	10463	322
HLA-B*15:03	YGFQPTNGVGY	YGFRPTYGVGH	368	10641
HLA-A*30:02	GFQPTNGVGY	GFRPTYGVGH	169	4730
HLA-A*30:02	QSYGFQPTNGVGY	QSYGFRPTYGVGH	258	7114
HLA-B*15:03	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	113	2917
HLA-B*53:01	FPLQSYGFQPTN	FPLQSYGFRPTY	8451	351
HLA-C*16:01	NGVGYQPY	YGVGHQPY	8669	488
HLA-C*07:02	FQPTNGVGYQPY	FRPTYGVGHQPY	6169	356
HLA-B*57:01	QSYGFQPTN	QSYGFRPTY	4070	293
HLA-A*30:01	QSYGFQPTN	QSYGFRPTY	3720	308
HLA-A*02:11	NGVGYQPYRV	YGVGHQPYRV	5989	500
HLA-B*15:03	NGVGYQPY	YGVGHQPY	4677	418
HLA-B*57:01	LQSYGFQPTN	LQSYGFRPTY	3839	375
HLA-C*14:02	FQPTNGVGYQPY	FRPTYGVGHQPY	3107	366
HLA-A*29:02	TNGVGYQPY	TYGVGHQPY	2031	243
HLA-A*31:01	TNGVGYQPYR	TYGVGHQPYR	811	103
HLA-A*33:03	TNGVGYQPYR	TYGVGHQPYR	573	79
HLA-B*15:03	FQPTNGVGYQPY	FRPTYGVGHQPY	183	1236
HLA-A*31:01	QPTNGVGYQPYR	RPTYGVGHQPYR	3100	478
HLA-B*58:01	QSYGFQPTN	QSYGFRPTY	1532	249
HLA-A*23:01	SYGFQPTNGV	SYGFRPTYGV	2697	483
HLA-A*02:11	YGFQPTNGV	YGFRPTYGV	307	59
HLA-B*15:03	LQSYGFQPTNGV	LQSYGFRPTYGV	1971	442
HLA-A*68:01	PTNGVGYQPYR	PTYGVGHQPYR	1073	303
HLA-A*33:03	PTNGVGYQPYR	PTYGVGHQPYR	1345	417
HLA-A*02:02	YGFQPTNGV	YGFRPTYGV	868	281
HLA-A*31:01	GFQPTNGVGYQPYR	GFRPTYGVGHQPYR	616	201
HLA-A*29:02	PTNGVGYQPY	PTYGVGHQPY	1211	401
HLA-A*31:01	PTNGVGYQPYR	PTYGVGHQPYR	1377	470
HLA-A*02:06	YGFQPTNGV	YGFRPTYGV	174	61
HLA-A*68:01	TNGVGYQPYR	TYGVGHQPYR	263	740
HLA-A*68:02	YGFQPTNGV	YGFRPTYGV	73	26
HLA-C*12:03	YGFQPTNGV	YGFRPTYGV	127	355
HLA-A*02:06	QSYGFQPTNGV	QSYGFRPTYGV	1121	425
HLA-A*68:02	QSYGFQPTNGV	QSYGFRPTYGV	122	47
HLA-A*30:02	PTNGVGYQPY	PTYGVGHQPY	465	205
HLA-A*31:01	NGVGYQPYR	YGVGHQPYR	438	207

Y505H

Reference  CNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKS
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    CNGVAGVNCYFPLQSYGFRPTYGVGHQPYRVVVLSFELLHAPATVCGPKKS

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*14:02	TNGVGYQPY	TYGVGHQPY	5468	16
HLA-B*15:01	FQPTNGVGY	FRPTYGVGH	166	29043
HLA-B*15:03	FQPTNGVGY	FRPTYGVGH	63	6390
HLA-A*29:02	GFQPTNGVGY	GFRPTYGVGH	188	18753
HLA-B*15:01	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	180	8335
HLA-C*14:02	GYQPYRVVV	GHQPYRVVV	78	3539
HLA-B*15:01	FQPTNGVGYQPY	FRPTYGVGHQPY	290	11634
HLA-C*03:02	NGVGYQPY	YGVGHQPY	10463	322
HLA-A*24:02	GYQPYRVVVLSF	GHQPYRVVVLSF	220	6955
HLA-A*23:01	GYQPYRVVVLSF	GHQPYRVVVLSF	107	3241
HLA-B*15:03	YGFQPTNGVGY	YGFRPTYGVGH	368	10641
HLA-A*30:02	GFQPTNGVGY	GFRPTYGVGH	169	4730
HLA-A*30:02	QSYGFQPTNGVGY	QSYGFRPTYGVGH	258	7114
HLA-B*15:03	LQSYGFQPTNGVGY	LQSYGFRPTYGVGH	113	2917
HLA-C*14:02	GYQPYRVVVL	GHQPYRVVVL	213	4430
HLA-C*14:02	VGYQPYRVVV	VGHQPYRVVV	413	7561
HLA-C*16:01	NGVGYQPY	YGVGHQPY	8669	488
HLA-C*07:02	FQPTNGVGYQPY	FRPTYGVGHQPY	6169	356
HLA-A*02:11	YQPYRVVVL	HQPYRVVVL	332	4009
HLA-A*02:11	NGVGYQPYRV	YGVGHQPYRV	5989	500
HLA-B*15:03	NGVGYQPY	YGVGHQPY	4677	418
HLA-A*02:06	YQPYRVVVL	HQPYRVVVL	223	2206
HLA-C*14:02	FQPTNGVGYQPY	FRPTYGVGHQPY	3107	366
HLA-A*29:02	TNGVGYQPY	TYGVGHQPY	2031	243
HLA-A*31:01	TNGVGYQPYR	TYGVGHQPYR	811	103
HLA-A*02:02	YQPYRVVVL	HQPYRVVVL	360	2683
HLA-A*33:03	TNGVGYQPYR	TYGVGHQPYR	573	79
HLA-B*15:03	FQPTNGVGYQPY	FRPTYGVGHQPY	183	1236
HLA-A*31:01	QPTNGVGYQPYR	RPTYGVGHQPYR	3100	478
HLA-C*01:02	YQPYRVVVL	HQPYRVVVL	41	241
HLA-A*02:06	YQPYRVVVLSFEL	HQPYRVVVLSFEL	430	2495
HLA-C*03:03	YQPYRVVVL	HQPYRVVVL	410	2128
HLA-C*03:04	YQPYRVVVL	HQPYRVVVL	410	2128
HLA-C*07:02	YQPYRVVVL	HQPYRVVVL	135	590
HLA-C*12:03	YQPYRVVVL	HQPYRVVVL	132	555
HLA-A*68:01	PTNGVGYQPYR	PTYGVGHQPYR	1073	303
HLA-C*14:02	YQPYRVVVL	HQPYRVVVL	102	347
HLA-C*16:01	YQPYRVVVL	HQPYRVVVL	214	726
HLA-A*33:03	PTNGVGYQPYR	PTYGVGHQPYR	1345	417
HLA-A*31:01	GFQPTNGVGYQPYR	GFRPTYGVGHQPYR	616	201
HLA-A*29:02	PTNGVGYQPY	PTYGVGHQPY	1211	401
HLA-C*12:03	VGYQPYRVV	VGHQPYRVV	398	1196
HLA-A*31:01	PTNGVGYQPYR	PTYGVGHQPYR	1377	470
HLA-A*68:01	TNGVGYQPYR	TYGVGHQPYR	263	740
HLA-A*23:01	YQPYRVVVLSF	HQPYRVVVLSF	465	1280
HLA-A*30:02	PTNGVGYQPY	PTYGVGHQPY	465	205
HLA-A*31:01	NGVGYQPYR	YGVGHQPYR	438	207

D614G

Reference  PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*05:01	YQDVNCTEV	YQGVNCTEV	82	8597
HLA-C*08:02	YQDVNCTEV	YQGVNCTEV	89	8066
HLA-A*02:11	DVNCTEVPV	GVNCTEVPV	2140	72
HLA-A*02:02	DVNCTEVPV	GVNCTEVPV	4099	213
HLA-A*68:02	DVNCTEVPV	GVNCTEVPV	36	659
HLA-A*02:06	DVNCTEVPV	GVNCTEVPV	3807	208
HLA-A*68:02	QVAVLYQDV	QVAVLYQGV	183	13
HLA-A*68:02	NQVAVLYQDV	NQVAVLYQGV	650	52
HLA-A*02:06	QVAVLYQDV	QVAVLYQGV	1312	148
HLA-A*02:11	QVAVLYQDV	QVAVLYQGV	2169	268
HLA-A*68:02	NTSNQVAVLYQDV	NTSNQVAVLYQGV	1858	231
HLA-A*68:02	TSNQVAVLYQDV	TSNQVAVLYQGV	3281	457
HLA-A*02:02	QVAVLYQDV	QVAVLYQGV	1107	188
HLA-A*02:01	YQDVNCTEV	YQGVNCTEV	75	315
HLA-A*02:06	YQDVNCTEV	YQGVNCTEV	17	57
HLA-A*02:11	VLYQDVNCT	VLYQGVNCT	224	74
HLA-A*02:11	YQDVNCTEV	YQGVNCTEV	31	93
HLA-A*02:11	VLYQDVNCTEVP	VLYQGVNCTEVP	1000	402
HLA-A*02:02	NQVAVLYQDV	NQVAVLYQGV	269	116
HLA-A*02:11	AVLYQDVNCTEV	AVLYQGVNCTEV	500	238
HLA-A*02:01	NQVAVLYQDV	NQVAVLYQGV	558	267
HLA-A*02:06	NQVAVLYQDV	NQVAVLYQGV	36	18

H655Y

Reference  TPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNS
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    TPTWRVYSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTKS

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*15:01	RAGCLIGAEH	RAGCLIGAEY	7377	156
HLA-A*30:02	RAGCLIGAEH	RAGCLIGAEY	1881	43
HLA-B*15:01	FQTRAGCLIGAEH	FQTRAGCLIGAEY	7512	178
HLA-B*15:03	FQTRAGCLIGAEH	FQTRAGCLIGAEY	5076	125
HLA-A*30:02	AGCLIGAEH	AGCLIGAEY	5331	179
HLA-B*15:03	RAGCLIGAEH	RAGCLIGAEY	5254	179
HLA-A*30:02	QTRAGCLIGAEH	QTRAGCLIGAEY	8425	342
HLA-B*15:03	AGCLIGAEH	AGCLIGAEY	9025	461
HLA-A*02:11	HVNNSYECDIPI	YVNNSYECDIPI	1593	109
HLA-A*02:01	HVNNSYECDIPI	YVNNSYECDIPI	2384	226
HLA-A*02:06	HVNNSYECDIPI	YVNNSYECDIPI	837	92
HLA-A*02:02	HVNNSYECDIPI	YVNNSYECDIPI	1466	187
HLA-A*02:11	CLIGAEHV	CLIGAEYV	983	221
HLA-B*15:03	AEHVNNSY	AEYVNNSY	986	267
HLA-B*18:01	AEHVNNSY	AEYVNNSY	902	286

N679K

Reference  EHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENS
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    EYVNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIAYTMSLGAENS

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*30:01	NSPRRARSV	KSHRRARSV	7195	22
HLA-A*68:01	CASYQTQTN	CASYQTQTK	28102	123
HLA-B*07:02	TNSPRRARSVA	TKSHRRARSVA	125	19196
HLA-B*07:02	NSPRRARSVA	KSHRRARSVA	69	5330
HLA-A*11:01	CASYQTQTN	CASYQTQTK	32767	431
HLA-B*07:02	TQTNSPRRARSVA	TQTKSHRRARSVA	270	17591
HLA-B*07:02	QTNSPRRARSVA	QTKSHRRARSVA	249	13621
HLA-B*42:01	TNSPRRARSVA	TKSHRRARSVA	437	22045
HLA-B*42:01	NSPRRARSVA	KSHRRARSVA	217	9050
HLA-A*30:01	NSPRRARSVAS	KSHRRARSVAS	4546	185
HLA-A*30:01	NSPRRARSVA	KSHRRARSVA	630	27
HLA-C*15:02	NSPRRARSV	KSHRRARSV	4094	215
HLA-A*68:01	QTNSPRRAR	QTKSHRRAR	95	1735
HLA-C*14:02	SYQTQTNSP	SYQTQTKSH	3691	214
HLA-A*68:01	TQTNSPRRAR	TQTKSHRRAR	254	4244
HLA-A*68:01	CASYQTQTNSPR	CASYQTQTKSHR	142	713
HLA-C*16:01	NSPRRARSV	KSHRRARSV	1247	297
HLA-A*11:01	ASYQTQTNSPR	ASYQTQTKSHR	346	1426
HLA-A*31:01	QTQTNSPRR	QTQTKSHRR	355	96
HLA-A*33:03	YQTQTNSPRR	YQTQTKSHRR	967	273
HLA-A*33:03	YQTQTNSPRRAR	YQTQTKSHRRAR	306	1077
HLA-A*31:01	YQTQTNSPRR	YQTQTKSHRR	965	276
HLA-A*33:03	TQTNSPRRAR	TQTKSHRRAR	195	659
HLA-A*33:03	QTQTNSPRR	QTQTKSHRR	445	143
HLA-A*31:01	ASYQTQTNSPRR	ASYQTQTKSHRR	433	144
HLA-A*33:03	QTNSPRRAR	QTKSHRRAR	48	144
HLA-A*30:01	QTNSPRRAR	QTKSHRRAR	1146	463
HLA-A*31:01	YQTQTNSPRRAR	YQTQTKSHRRAR	317	771
HLA-A*31:01	SYQTQTNSPR	SYQTQTKSHR	62	147
HLA-A*33:03	SYQTQTNSPR	SYQTQTKSHR	130	304
HLA-A*31:01	TQTNSPRRAR	TQTKSHRRAR	144	336
HLA-A*31:01	QTNSPRRAR	QTKSHRRAR	34	79
HLA-A*31:01	ASYQTQTNSPR	ASYQTQTKSHR	139	315
HLA-A*31:01	YQTQTNSPR	YQTQTKSHR	441	982
HLA-A*31:01	SYQTQTNSPRRAR	SYQTQTKSHRRAR	193	427
HLA-A*33:03	SYQTQTNSPRRAR	SYQTQTKSHRRAR	448	972

P681H

Reference  VNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVA
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    VNNSYECDIPIGAGICASYQTQTKSHRRARSVASQSIIAYTMSLGAENSVA

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*07:02	SPRRARSVA	SHRRARSVA	4	4721
HLA-B*42:01	SPRRARSVA	SHRRARSVA	8	6169
HLA-B*07:02	SPRRARSVASQSI	SHRRARSVASQSI	17	11433
HLA-B*07:02	SPRRARSV	SHRRARSV	24	12728
HLA-B*07:02	SPRRARSVAS	SHRRARSVAS	37	14758
HLA-A*30:01	NSPRRARSV	KSHRRARSV	7195	22
HLA-B*07:02	SPRRARSVASQSII	SHRRARSVASQSII	55	17736
HLA-B*42:01	SPRRARSV	SHRRARSV	38	11622
HLA-B*42:01	SPRRARSVASQSI	SHRRARSVASQSI	48	14418
HLA-B*07:02	TNSPRRARSVA	TKSHRRARSVA	125	19196
HLA-B*42:01	SPRRARSVAS	SHRRARSVAS	158	17461
HLA-B*07:02	NSPRRARSVA	KSHRRARSVA	69	5330
HLA-B*42:01	SPRRARSVASQSII	SHRRARSVASQSII	321	21521
HLA-B*07:02	TQTNSPRRARSVA	TQTKSHRRARSVA	270	17591
HLA-B*07:02	QTNSPRRARSVA	QTKSHRRARSVA	249	13621
HLA-B*42:01	TNSPRRARSVA	TKSHRRARSVA	437	22045
HLA-B*42:01	NSPRRARSVA	KSHRRARSVA	217	9050
HLA-A*30:01	NSPRRARSVAS	KSHRRARSVAS	4546	185
HLA-A*30:01	NSPRRARSVA	KSHRRARSVA	630	27
HLA-C*15:02	NSPRRARSV	KSHRRARSV	4094	215
HLA-A*68:01	QTNSPRRAR	QTKSHRRAR	95	1735
HLA-C*14:02	SYQTQTNSP	SYQTQTKSH	3691	214
HLA-A*68:01	TQTNSPRRAR	TQTKSHRRAR	254	4244
HLA-B*08:01	SPRRARSVA	SHRRARSVA	372	3831
HLA-B*08:01	SPRRARSV	SHRRARSV	428	2448
HLA-A*68:01	CASYQTQTNSPR	CASYQTQTKSHR	142	713
HLA-C*16:01	NSPRRARSV	KSHRRARSV	1247	297
HLA-A*11:01	ASYQTQTNSPR	ASYQTQTKSHR	346	1426
HLA-A*31:01	QTQTNSPRR	QTQTKSHRR	355	96
HLA-A*33:03	YQTQTNSPRR	YQTQTKSHRR	967	273
HLA-A*33:03	YQTQTNSPRRAR	YQTQTKSHRRAR	306	1077
HLA-A*31:01	YQTQTNSPRR	YQTQTKSHRR	965	276
HLA-A*33:03	TQTNSPRRAR	TQTKSHRRAR	195	659
HLA-A*33:03	QTQTNSPRR	QTQTKSHRR	445	143
HLA-A*31:01	ASYQTQTNSPRR	ASYQTQTKSHRR	433	144
HLA-A*33:03	QTNSPRRAR	QTKSHRRAR	48	144
HLA-A*30:01	QTNSPRRAR	QTKSHRRAR	1146	463
HLA-A*31:01	YQTQTNSPRRAR	YQTQTKSHRRAR	317	771
HLA-A*31:01	SYQTQTNSPR	SYQTQTKSHR	62	147
HLA-A*33:03	SYQTQTNSPR	SYQTQTKSHR	130	304
HLA-A*31:01	TQTNSPRRAR	TQTKSHRRAR	144	336
HLA-A*31:01	QTNSPRRAR	QTKSHRRAR	34	79
HLA-A*31:01	ASYQTQTNSPR	ASYQTQTKSHR	139	315
HLA-A*31:01	YQTQTNSPR	YQTQTKSHR	441	982
HLA-A*31:01	SYQTQTNSPRRAR	SYQTQTKSHRRAR	193	427
HLA-A*33:03	SYQTQTNSPRRAR	SYQTQTKSHRRAR	448	972

N764K

Reference  TMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIY
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    TMYICGDSTECSNLLLQYGSFCTQLKRALTGIAVEQDKNTQEVFAQVKQIY

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*03:02	LLQYGSFCTQLN	LLQYGSFCTQLK	29153	102
HLA-A*11:01	LQYGSFCTQLN	LQYGSFCTQLK	32767	191
HLA-A*03:01	LQYGSFCTQLN	LQYGSFCTQLK	28204	214
HLA-A*68:01	YGSFCTQLN	YGSFCTQLK	23773	187
HLA-A*03:02	LQYGSFCTQLN	LQYGSFCTQLK	31227	331
HLA-A*03:02	LLLQYGSFCTQLN	LLLQYGSFCTQLK	32767	377
HLA-A*11:01	YGSFCTQLN	YGSFCTQLK	32767	386
HLA-A*03:01	LLQYGSFCTQLN	LLQYGSFCTQLK	29424	350
HLA-A*03:01	LLLQYGSFCTQLN	LLLQYGSFCTQLK	32355	410
HLA-A*02:11	QLNRALTGI	QLKRALTGI	27	440
HLA-A*02:11	FCTQLNRALTGI	FCTQLKRALTGI	63	898
HLA-B*08:01	CTQLNRAL	CTQLKRAL	3325	279
HLA-A*02:01	FCTQLNRALTGI	FCTQLKRALTGI	493	4878
HLA-A*02:02	FCTQLNRALTGI	FCTQLKRALTGI	62	503
HLA-A*02:02	QLNRALTGI	QLKRALTGI	45	307
HLA-C*03:03	FCTQLNRAL	FCTQLKRAL	138	752
HLA-C*03:04	FCTQLNRAL	FCTQLKRAL	138	752
HLA-A*02:02	TQLNRALTGI	TQLKRALTGI	180	882
HLA-A*02:11	TQLNRALTGI	TQLKRALTGI	267	1284
HLA-B*27:05	NRALTGIAV	KRALTGIAV	1251	276
HLA-A*02:11	QLNRALTGIAV	QLKRALTGIAV	259	1123
HLA-C*03:02	FCTQLNRAL	FCTQLKRAL	361	1438
HLA-C*14:02	SFCTQLNRAL	SFCTQLKRAL	234	511
HLA-A*02:06	TQLNRALTGI	TQLKRALTGI	54	113

D796Y

Reference  AVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDL
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    AVEQDKNTQEVFAQVKQIYKTPPIKYFGGFNFSQILPDPSKPSKRSFIEDL

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*23:01	KDFGGFNF	KYFGGFNF	15419	23
HLA-A*23:01	KDFGGFNFSQI	KYFGGFNFSQI	15932	37
HLA-A*24:02	KDFGGFNF	KYFGGFNF	21008	55
HLA-C*14:02	IYKTPPIKD	IYKTPPIKY	16538	55
HLA-A*24:02	KDFGGFNFSQI	KYFGGFNFSQI	20251	68
HLA-B*15:03	KQIYKTPPIKD	KQIYKTPPIKY	7773	29
HLA-A*23:01	KDFGGFNFSQIL	KYFGGFNFSQIL	15831	63
HLA-C*14:02	KDFGGFNF	KYFGGFNF	22081	90
HLA-A*24:02	KDFGGFNFSQIL	KYFGGFNFSQIL	21565	126
HLA-B*15:01	KQIYKTPPIKD	KQIYKTPPIKY	22193	141
HLA-C*14:02	KDFGGFNFSQIL	KYFGGFNFSQIL	14873	165
HLA-A*30:01	KDFGGFNFS	KYFGGFNFS	9496	156
HLA-C*14:02	DFGGFNFSQIL	YFGGFNFSQIL	6960	139
HLA-A*30:02	KQIYKTPPIKD	KQIYKTPPIKY	15917	386
HLA-B*15:03	VKQIYKTPPIKD	VKQIYKTPPIKY	16977	446
HLA-B*15:03	IKDFGGFNF	IKYFGGFNF	97	4
HLA-B*15:03	PIKDFGGFNF	PIKYFGGFNF	7546	321
HLA-B*15:03	TPPIKDFGGFNF	TPPIKYFGGFNF	10071	484
HLA-B*15:03	KTPPIKDFGGFNF	KTPPIKYFGGFNF	6427	331
HLA-A*32:01	KTPPIKDFGGFNF	KTPPIKYFGGFNF	6031	381
HLA-A*23:01	DFGGFNFSQI	YFGGFNFSQI	5424	461
HLA-B*15:03	YKTPPIKDFGGFNF	YKTPPIKYFGGFNF	2588	394
HLA-C*06:02	YKTPPIKDF	YKTPPIKYF	2488	460
HLA-C*07:02	YKTPPIKDF	YKTPPIKYF	1804	378
HLA-A*23:01	IYKTPPIKDF	IYKTPPIKYF	273	98
HLA-A*24:02	IYKTPPIKDF	IYKTPPIKYF	375	147
HLA-C*14:02	IYKTPPIKDF	IYKTPPIKYF	341	158
HLA-B*15:03	YKTPPIKDF	YKTPPIKYF	92	43

Q954H

Reference  SAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLND
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    SAIGKIQDSLSSTASALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLND

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*38:01	NQNAQALNTL	NHNAQALNTL	2805	97
HLA-A*68:02	DVVNQNAQA	DVVNHNAQA	703	344

N969K

Reference  ALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQID
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    ALGKLQDVVNHNAQALNTLVKQLSSKFGAISSVLNDILSRLDKVEAEVQID

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*03:01	TLVKQLSSN	TLVKQLSSK	29207	56
HLA-A*68:01	NTLVKQLSSN	NTLVKQLSSK	28063	86
HLA-A*11:01	TLVKQLSSN	TLVKQLSSK	32767	125
HLA-A*03:02	TLVKQLSSN	TLVKQLSSK	31915	131
HLA-A*11:01	NTLVKQLSSN	NTLVKQLSSK	32767	147
HLA-A*03:01	ALNTLVKQLSSN	ALNTLVKQLSSK	32767	154
HLA-A*03:02	ALNTLVKQLSSN	ALNTLVKQLSSK	32767	304
HLA-A*03:01	NTLVKQLSSN	NTLVKQLSSK	32767	482
HLA-A*68:02	SNFGAISSV	SKFGAISSV	117	5329
HLA-B*15:03	SNFGAISSVL	SKFGAISSVL	688	25
HLA-B*08:01	QLSSNFGAI	QLSSKFGAI	4755	265
HLA-C*15:02	SNFGAISSV	SKFGAISSV	374	5700
HLA-A*30:01	SSNFGAISS	SSKFGAISS	1320	88
HLA-B*15:03	SNFGAISSV	SKFGAISSV	727	49
HLA-A*30:01	SSNFGAISSV	SSKFGAISSV	587	71
HLA-A*02:06	KQLSSNFGA	KQLSSKFGA	62	269
HLA-A*02:01	KQLSSNFGAI	KQLSSKFGAI	407	1567
HLA-A*02:11	KQLSSNFGA	KQLSSKFGA	228	807
HLA-A*02:06	SNFGAISSV	SKFGAISSV	361	1100
HLA-C*06:02	SNFGAISSV	SKFGAISSV	1423	485
HLA-A*02:11	KQLSSNFGAI	KQLSSKFGAI	152	428
HLA-A*02:06	KQLSSNFGAI	KQLSSKFGAI	58	159
HLA-A*02:02	KQLSSNFGAI	KQLSSKFGAI	261	668
HLA-B*15:03	KQLSSNFGA	KQLSSKFGA	497	1076

N protein

P13L

Reference  MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSK
           |||||||||||| |||||||||||||||||||||||||
Mutated    MSDNGPQNQRNALRITFGGPSDSTGSNQNGECSGARSK

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*42:01	MSDNGPQNQRNAP	MSDNGPQNQRNAL	11146	27
HLA-B*07:02	GPQNQRNAP	GPQNQRNAL	2464	6
HLA-B*42:01	GPQNQRNAP	GPQNQRNAL	3035	8
HLA-B*07:02	SDNGPQNQRNAP	SDNGPQNQRNAL	11353	32
HLA-B*42:01	SDNGPQNQRNAP	SDNGPQNQRNAL	13042	39
HLA-B*42:01	NGPQNQRNAP	NGPQNQRNAL	12875	41
HLA-B*07:02	MSDNGPQNQRNAP	MSDNGPQNQRNAL	13138	58
HLA-B*07:02	NGPQNQRNAP	NGPQNQRNAL	14898	72
HLA-B*07:02	APRITFGGPS	ALRITFGGPS	191	17534
HLA-B*42:01	PQNQRNAP	PQNQRNAL	30142	436
HLA-B*07:02	GPQNQRNAPR	GPQNQRNALR	13964	345
HLA-B*27:05	QRNAPRITF	QRNALRITF	509	83
HLA-B*27:05	NQRNAPRITF	NQRNALRITF	1716	350
HLA-B*27:05	GPQNQRNAPRITF	GPQNQRNALRITF	2076	442
HLA-B*15:01	NQRNAPRITF	NQRNALRITF	96	40

R32C

Reference  QNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALT
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    QNQRNALRITFGGPSDSTGSNQNGECSGARSKQRRPQGLPNNTASWFTALT

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:01	STGSNQNGER	STGSNQNGEC	137	32767
HLA-A*31:01	RSGARSKQR	CSGARSKQR	77	451
HLA-A*31:01	RSGARSKQRR	CSGARSKQRR	254	980

203-204 RG->KR

Reference  GGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ
           |||||||||||||||||||||||||  |||||||||||||||||||||||||
Mutated    GGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMAGNGGDAALALLLLDRLNQ

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:01	NSTPGSSRG	NSTPGSSKR	32767	34
HLA-A*33:03	NSTPGSSRG	NSTPGSSKR	32767	300
HLA-B*15:03	SRGTSPARM	SKRTSPARM	2669	106
HLA-A*31:01	RNSTPGSSRG	RNSTPGSSKR	7013	340
HLA-A*30:01	SSRNSTPGSSR	SSRNSTPGSSK	356	20
HLA-A*30:01	GTSPARMAG	RTSPARMAG	493	30
HLA-A*31:01	SSRNSTPGSSRG	SSRNSTPGSSKR	3757	231
HLA-A*30:01	GTSPARMAGNGG	RTSPARMAGNGG	6051	411
HLA-A*31:01	SSRNSTPGSSR	SSRNSTPGSSK	127	1853
HLA-A*30:01	NSSRNSTPGSSR	NSSRNSTPGSSK	4255	353
HLA-A*30:01	RNSTPGSSR	RNSTPGSSK	2120	188
HLA-A*31:01	RNSTPGSSR	RNSTPGSSK	123	1335
HLA-A*03:01	SSRNSTPGSSR	SSRNSTPGSSK	4753	442
HLA-A*11:01	SSRNSTPGSSR	SSRNSTPGSSK	2231	263
HLA-A*68:01	NSSRNSTPGSSR	NSSRNSTPGSSK	270	1385
HLA-A*30:01	GSSRGTSPA	GSSKRTSPA	555	145
HLA-A*30:01	SSRNSTPGSSRG	SSRNSTPGSSKR	1625	469
HLA-A*30:01	SSRGTSPARMAG	SSKRTSPARMAG	449	1031

S413R

Reference  KQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
           ||||||||||||||||||||||||| ||||||
Mutated    KQQTVTLLPAADLDDFSKQLQQSMSRADSTQA

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*31:01	KQLQQSMSS	KQLQQSMSR	20016	55
HLA-A*30:01	MSSADSTQA	MSRADSTQA	2953	37
HLA-A*33:03	DFSKQLQQSMSS	DFSKQLQQSMSR	32767	494
HLA-B*15:03	KQLQQSMSS	KQLQQSMSR	236	5105
HLA-A*30:01	SMSSADSTQA	SMSRADSTQA	6453	416
HLA-A*02:02	FSKQLQQSMSSA	FSKQLQQSMSRA	345	1138
HLA-A*02:11	FSKQLQQSMSSA	FSKQLQQSMSRA	353	1118
HLA-A*02:11	QLQQSMSSA	QLQQSMSRA	436	1175
HLA-A*02:02	SMSSADSTQA	SMSRADSTQA	355	725

M protein

D3N

Reference  MADSNGTITVEELKKLLEQWNLVIGFLF
           || |||||||||||||||||||||||||
Mutated    MANSNGTITVEELKKLLEEWNLVIGFLF

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*05:01	MADSNGTITV	MANSNGTITV	73	6019
HLA-C*08:02	MADSNGTI	MANSNGTI	136	10296
HLA-C*08:02	MADSNGTITV	MANSNGTITV	94	6648
HLA-C*05:01	MADSNGTI	MANSNGTI	235	12988
HLA-C*08:02	MADSNGTITVEEL	MANSNGTITVEEL	237	12268
HLA-C*05:01	MADSNGTITVEEL	MANSNGTITVEEL	380	15464
HLA-C*12:03	MADSNGTITV	MANSNGTITV	454	169
HLA-C*16:01	MADSNGTITV	MANSNGTITV	308	151

Q19E

Reference  MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNR
           |||||||||||||||||| |||||||||||||||||||||||||
Mutated    MANSNGTITVEELKKLLEEWNLVIGFLFLTWICLLQFAYANRNR

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*44:03	EQWNLVIGF	EEWNLVIGF	1268	15
HLA-B*44:02	EQWNLVIGF	EEWNLVIGF	2396	34
HLA-B*44:03	EQWNLVIGFLF	EEWNLVIGFLF	5200	85
HLA-B*44:03	EQWNLVIGFL	EEWNLVIGFL	8038	135
HLA-B*44:03	EQWNLVIGFLFLTW	EEWNLVIGFLFLTW	5966	126
HLA-B*44:02	EQWNLVIGFLF	EEWNLVIGFLF	6607	162
HLA-B*44:02	EQWNLVIGFL	EEWNLVIGFL	9054	237
HLA-B*44:02	EQWNLVIGFLFLTW	EEWNLVIGFLFLTW	5922	170
HLA-B*15:01	LEQWNLVIGF	LEEWNLVIGF	256	8100
HLA-B*18:01	EQWNLVIGF	EEWNLVIGF	482	16
HLA-A*02:06	EQWNLVIGFL	EEWNLVIGFL	418	10916
HLA-B*15:03	EQWNLVIGF	EEWNLVIGF	101	2397
HLA-B*18:01	EQWNLVIGFLF	EEWNLVIGFLF	3471	190
HLA-B*40:02	EQWNLVIGFL	EEWNLVIGFL	3775	245
HLA-B*15:03	LEQWNLVIGF	LEEWNLVIGF	88	1227
HLA-B*40:02	EQWNLVIGF	EEWNLVIGF	1765	242
HLA-B*15:03	KLLEQWNLVIGF	KLLEEWNLVIGF	365	2285
HLA-A*02:11	LLEQWNLVI	LLEEWNLVI	142	24
HLA-A*02:11	LLEQWNLVIGFL	LLEEWNLVIGFL	1120	210
HLA-A*02:01	LLEQWNLVI	LLEEWNLVI	644	131
HLA-A*29:02	QWNLVIGFLF	EWNLVIGFLF	159	780
HLA-A*24:02	QWNLVIGFLF	EWNLVIGFLF	53	246
HLA-A*23:01	QWNLVIGFLF	EWNLVIGFLF	18	79
HLA-A*02:02	LLEQWNLVI	LLEEWNLVI	781	187
HLA-A*02:06	LLEQWNLVI	LLEEWNLVI	1869	487
HLA-B*40:02	LEQWNLVI	LEEWNLVI	367	1301
HLA-A*02:02	LLEQWNLVIGFL	LLEEWNLVIGFL	1047	329

A63T

Reference  AYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLV
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    AYANRNRFLYIIKLIFLWLLWPVTLTCFVLAAVYRINWITGGIAIAMACLV

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*35:01	LACFVLAAVY	LTCFVLAAVY	121	1943
HLA-A*68:01	ACFVLAAVYR	TCFVLAAVYR	694	80
HLA-B*35:01	ACFVLAAVY	TCFVLAAVY	2289	331
HLA-C*03:02	LACFVLAAVY	LTCFVLAAVY	145	935
HLA-C*16:01	LACFVLAAV	LTCFVLAAV	346	1994
HLA-A*02:01	WLLWPVTLA	WLLWPVTLT	33	190
HLA-A*02:02	WLLWPVTLA	WLLWPVTLT	32	180
HLA-A*02:11	LWLLWPVTLA	LWLLWPVTLT	66	363
HLA-A*02:11	IFLWLLWPVTLA	IFLWLLWPVTLT	190	1034
HLA-A*02:11	LLWPVTLA	LLWPVTLT	242	1313
HLA-A*33:03	ACFVLAAVYR	TCFVLAAVYR	252	47
HLA-A*02:02	LWLLWPVTLA	LWLLWPVTLT	304	1623
HLA-C*12:03	LACFVLAAV	LTCFVLAAV	214	1116
HLA-A*02:06	WLLWPVTLA	WLLWPVTLT	60	302
HLA-A*02:01	LWLLWPVTLA	LWLLWPVTLT	228	1142
HLA-A*02:02	TLACFVLAAVY	TLTCFVLAAVY	337	1467
HLA-A*02:02	VTLACFVLAAV	VTLTCFVLAAV	160	678
HLA-A*68:02	LACFVLAAV	LTCFVLAAV	44	11
HLA-A*02:02	PVTLACFVLAAV	PVTLTCFVLAAV	479	1907
HLA-A*02:11	LIFLWLLWPVTLA	LIFLWLLWPVTLT	406	1602
HLA-A*02:02	TLACFVLAAVYRI	TLTCFVLAAVYRI	293	1148
HLA-A*02:11	LACFVLAAV	LTCFVLAAV	122	32
HLA-A*02:11	WLLWPVTLA	WLLWPVTLT	6	22
HLA-B*15:03	LACFVLAAVY	LTCFVLAAVY	81	284
HLA-A*68:02	TLACFVLAA	TLTCFVLAA	395	1376
HLA-A*02:02	FLWLLWPVTLACFV	FLWLLWPVTLTCFV	485	146
HLA-A*68:01	LACFVLAAVY	LTCFVLAAVY	1184	359
HLA-A*02:02	TLACFVLAA	TLTCFVLAA	13	41
HLA-A*02:11	TLACFVLAAVY	TLTCFVLAAVY	310	929
HLA-A*02:02	FLWLLWPVTLA	FLWLLWPVTLT	291	867
HLA-A*02:11	KLIFLWLLWPVTLA	KLIFLWLLWPVTLT	290	859
HLA-A*29:02	LACFVLAAVY	LTCFVLAAVY	232	81
HLA-A*02:02	VTLACFVLAA	VTLTCFVLAA	100	285
HLA-A*02:06	TLACFVLAAV	TLTCFVLAAV	16	45
HLA-A*68:02	VTLACFVLAA	VTLTCFVLAA	481	1321
HLA-B*15:01	TLACFVLAAVY	TLTCFVLAAVY	414	1136
HLA-A*23:01	LWPVTLACF	LWPVTLTCF	57	155
HLA-A*24:02	LWPVTLACF	LWPVTLTCF	49	133
HLA-A*02:11	FLWLLWPVTLA	FLWLLWPVTLT	25	67
HLA-A*02:06	TLACFVLAA	TLTCFVLAA	40	107
HLA-A*02:01	LACFVLAAV	LTCFVLAAV	471	179
HLA-A*02:02	TLACFVLAAV	TLTCFVLAAV	5	13
HLA-A*02:11	VTLACFVLAA	VTLTCFVLAA	97	245
HLA-A*02:01	FLWLLWPVTLA	FLWLLWPVTLT	107	269
HLA-A*02:06	FLWLLWPVTLA	FLWLLWPVTLT	495	1240
HLA-A*02:11	TLACFVLAA	TLTCFVLAA	8	20
HLA-A*02:11	VTLACFVLAAV	VTLTCFVLAAV	128	318
HLA-A*02:01	TLACFVLAAV	TLTCFVLAAV	11	27
HLA-A*02:02	LACFVLAAV	LTCFVLAAV	198	81
HLA-A*02:01	TLACFVLAA	TLTCFVLAA	25	61
HLA-A*02:01	VTLACFVLAAV	VTLTCFVLAAV	436	1029
HLA-B*07:02	WPVTLACFVL	WPVTLTCFVL	362	155
HLA-A*02:01	FLWLLWPVTLACFV	FLWLLWPVTLTCFV	329	142
HLA-A*02:11	TLACFVLAAV	TLTCFVLAAV	4	9
HLA-A*30:02	LACFVLAAVY	LTCFVLAAVY	218	98
HLA-A*02:06	LACFVLAAV	LTCFVLAAV	51	23
HLA-A*03:02	TLACFVLAAVYR	TLTCFVLAAVYR	480	1047
HLA-A*68:02	TLACFVLAAV	TLTCFVLAAV	26	12
HLA-B*42:01	WPVTLACFV	WPVTLTCFV	209	102
HLA-A*32:01	LLWPVTLACF	LLWPVTLTCF	427	212

E protein

T9I

Reference  MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAIL
           |||||||| |||||||||||||||||||||||||
Mutated    MYSFVSEEIGTLIVNSVLLFLAFVVFLLVTLAIL

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*24:02	MYSFVSEET	MYSFVSEEI	5532	18
HLA-A*23:01	MYSFVSEET	MYSFVSEEI	5604	31
HLA-C*16:01	YSFVSEET	YSFVSEEI	7157	226
HLA-C*14:02	MYSFVSEET	MYSFVSEEI	901	30
HLA-C*12:03	YSFVSEET	YSFVSEEI	10273	457
HLA-A*68:02	MYSFVSEET	MYSFVSEEI	3879	472
HLA-A*68:02	ETGTLIVNSV	EIGTLIVNSV	13	91
HLA-A*68:02	FVSEETGTLI	FVSEEIGTLI	247	60
HLA-A*68:02	TGTLIVNSV	IGTLIVNSV	491	1976
HLA-A*02:11	FVSEETGTLI	FVSEEIGTLI	307	89
HLA-B*40:01	EETGTLIVNSVL	EEIGTLIVNSVL	1578	499
HLA-A*02:01	FVSEETGTL	FVSEEIGTL	575	186
HLA-A*02:11	FVSEETGTL	FVSEEIGTL	97	32
HLA-A*02:06	FVSEETGTLI	FVSEEIGTLI	423	144
HLA-A*02:06	FVSEETGTL	FVSEEIGTL	120	41
HLA-A*02:01	FVSEETGTLI	FVSEEIGTLI	1293	442
HLA-A*02:06	FVSEETGTLIV	FVSEEIGTLIV	670	266
HLA-C*03:03	YSFVSEETGTL	YSFVSEEIGTL	293	711
HLA-C*03:04	YSFVSEETGTL	YSFVSEEIGTL	293	711
HLA-B*44:03	EETGTLIVNSVLLF	EEIGTLIVNSVLLF	877	375
HLA-A*02:11	FVSEETGTLIV	FVSEEIGTLIV	538	251
HLA-A*02:02	FVSEETGTLI	FVSEEIGTLI	85	40
HLA-A*68:02	YSFVSEETGTLI	YSFVSEEIGTLI	1008	485

NS3 protein

T223I

Reference  KDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHT
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    KDCVVLHSYFTSDYYQLYSTQLSTDIGVEHVTFFIYNKIVDEPEEHVQIHT

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*03:03	YSTQLSTDT	YSTQLSTDI	12349	241
HLA-C*03:04	YSTQLSTDT	YSTQLSTDI	12349	241
HLA-C*12:03	YSTQLSTDT	YSTQLSTDI	11751	430
HLA-C*16:01	YSTQLSTDT	YSTQLSTDI	7819	294
HLA-C*03:02	YSTQLSTDT	YSTQLSTDI	6466	347
HLA-A*02:06	YQLYSTQLSTDT	YQLYSTQLSTDI	4179	287
HLA-A*02:11	QLYSTQLSTDT	QLYSTQLSTDI	5151	437
HLA-A*68:01	TGVEHVTFFIYNK	IGVEHVTFFIYNK	213	1427
HLA-A*68:02	LSTDTGVEHV	LSTDIGVEHV	1765	265
HLA-A*68:02	TGVEHVTFFI	IGVEHVTFFI	212	1093
HLA-A*02:01	TQLSTDTGV	TQLSTDIGV	1084	283
HLA-A*02:01	YSTQLSTDTGV	YSTQLSTDIGV	1288	379
HLA-A*02:11	STQLSTDTGV	STQLSTDIGV	1485	443
HLA-A*25:01	DTGVEHVTFF	DIGVEHVTFF	180	596
HLA-A*02:11	TQLSTDTGV	TQLSTDIGV	436	133
HLA-A*02:06	STQLSTDTGV	STQLSTDIGV	264	83
HLA-A*02:11	YSTQLSTDTGV	YSTQLSTDIGV	1184	381
HLA-A*26:01	DTGVEHVTFF	DIGVEHVTFF	37	108
HLA-A*02:06	YSTQLSTDTGV	YSTQLSTDIGV	157	56
HLA-C*03:02	TGVEHVTFF	IGVEHVTFF	237	88
HLA-A*02:06	TQLSTDTGV	TQLSTDIGV	70	26
HLA-B*15:03	TGVEHVTFF	IGVEHVTFF	305	115
HLA-A*02:06	LYSTQLSTDTGV	LYSTQLSTDIGV	855	330
HLA-C*16:01	TGVEHVTFF	IGVEHVTFF	392	185

NS9b protein

P10S

Reference  MDPKISEMHPALRLVDPQIQLAVTRMENAVGRDQN
           ||||||||| |||||||||||||||||||||||||
Mutated    MDPKISEMHSALRLVDPQIQLAVTRMENAVGRDQN

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*42:01	HPALRLVDPQIQL	HSALRLVDPQIQL	148	15057
HLA-B*42:01	HPALRLVDPQI	HSALRLVDPQI	297	15937
HLA-A*02:11	PALRLVDPQIQL	SALRLVDPQIQL	3683	325
HLA-B*38:01	MHPALRLVDPQI	MHSALRLVDPQI	3064	395
HLA-A*02:02	PALRLVDPQIQL	SALRLVDPQIQL	2220	388
HLA-A*02:01	KISEMHPAL	KISEMHSAL	139	774
HLA-A*02:06	KISEMHPAL	KISEMHSAL	62	310
HLA-B*07:02	KISEMHPAL	KISEMHSAL	436	134
HLA-A*02:11	KISEMHPAL	KISEMHSAL	14	42
HLA-C*08:02	ISEMHPAL	ISEMHSAL	896	414
HLA-B*15:03	SEMHPALRL	SEMHSALRL	493	245

NS9c protein

G50N

Reference  AAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
           ||||||||||||||||||||||||| |||||||||||||||||||||||
Mutated    AAVKPLLVPHHVVATVQEIQLQAAVNELLLLEWLAMAVMLLLLCCCLTD

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*18:01	GELLLLEWLAM	NELLLLEWLAM	4979	247
HLA-B*18:01	GELLLLEW	NELLLLEW	3915	401
HLA-B*40:01	GELLLLEWL	NELLLLEWL	28	140
HLA-A*02:11	QLQAAVGEL	QLQAAVNEL	120	27
HLA-A*02:11	IQLQAAVGEL	IQLQAAVNEL	338	81
HLA-B*40:02	GELLLLEWL	NELLLLEWL	52	204
HLA-B*40:02	GELLLLEWLAM	NELLLLEWLAM	201	772
HLA-A*02:01	QLQAAVGEL	QLQAAVNEL	1290	358
HLA-A*02:02	QLQAAVGEL	QLQAAVNEL	92	28
HLA-B*58:01	VGELLLLEW	VNELLLLEW	330	1071
HLA-A*02:02	IQLQAAVGEL	IQLQAAVNEL	170	58
HLA-A*02:01	LQAAVGELL	LQAAVNELL	938	352
HLA-A*02:02	LQAAVGELL	LQAAVNELL	44	17
HLA-B*40:06	GELLLLEWLA	NELLLLEWLA	268	677
HLA-B*40:02	GELLLLEWLA	NELLLLEWLA	475	1195
HLA-A*02:11	LQAAVGELL	LQAAVNELL	386	154
HLA-A*02:01	IQLQAAVGEL	IQLQAAVNEL	1138	475
HLA-A*02:01	QLQAAVGELL	QLQAAVNELL	961	403
HLA-A*02:01	GELLLLEWLAMA	NELLLLEWLAMA	109	258
HLA-A*02:02	QLQAAVGELL	QLQAAVNELL	35	15
HLA-A*02:02	IQLQAAVGELL	IQLQAAVNELL	724	313
HLA-A*02:11	QLQAAVGELL	QLQAAVNELL	303	131
HLA-B*58:01	AVGELLLLEW	AVNELLLLEW	196	87
HLA-A*02:11	GELLLLEWLAMA	NELLLLEWLAMA	29	61
HLA-B*57:01	AVGELLLLEW	AVNELLLLEW	432	206
HLA-A*68:02	EIQLQAAVGELL	EIQLQAAVNELL	697	346

NSP1 protein

S135R

Reference  HVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQEN
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    HVGEIPVAYRKVLLRKNGNKGAGGHRYGADLKSFDLGDELGTDPYEDFQEN

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Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*58:01	HSYGADLKSF	HRYGADLKSF	59	18326
HLA-B*27:05	HSYGADLKSF	HRYGADLKSF	15851	60
HLA-B*57:01	HSYGADLKSF	HRYGADLKSF	158	23790
HLA-B*27:05	HSYGADLKSFDL	HRYGADLKSFDL	23268	171
HLA-B*15:01	HSYGADLKSF	HRYGADLKSF	94	6548
HLA-C*03:02	HSYGADLKSF	HRYGADLKSF	94	6500
HLA-C*16:01	HSYGADLKSF	HRYGADLKSF	329	14251
HLA-B*15:03	RKNGNKGAGGHSY	RKNGNKGAGGHRY	183	1186
HLA-B*15:03	NKGAGGHSY	NKGAGGHRY	129	774
HLA-B*15:03	GNKGAGGHSY	GNKGAGGHRY	478	2611

NSP3 protein

T24I

Reference  APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTE
           ||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    APTKVTFGDDTVIEVQGYKSVNIIFELDERIDKVLNEKCSAYTVELGTE

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*58:01	ITFELDERI	IIFELDERI	270	4454
HLA-B*15:03	VQGYKSVNIT	VQGYKSVNII	3702	395
HLA-A*02:01	ITFELDERI	IIFELDERI	1637	223
HLA-A*02:11	ITFELDERI	IIFELDERI	261	37
HLA-A*02:02	ITFELDERI	IIFELDERI	428	65
HLA-A*24:02	VQGYKSVNITF	VQGYKSVNIIF	194	811
HLA-A*24:02	IEVQGYKSVNITF	IEVQGYKSVNIIF	366	1499
HLA-A*68:02	ITFELDERI	IIFELDERI	253	1036
HLA-A*68:01	NITFELDER	NIIFELDER	68	20
HLA-A*33:03	NITFELDER	NIIFELDER	874	262
HLA-A*23:01	IEVQGYKSVNITF	IEVQGYKSVNIIF	191	635
HLA-C*03:03	YKSVNITFEL	YKSVNIIFEL	305	1007
HLA-C*03:04	YKSVNITFEL	YKSVNIIFEL	305	1007
HLA-A*23:01	VIEVQGYKSVNITF	VIEVQGYKSVNIIF	413	1337
HLA-A*23:01	VQGYKSVNITF	VQGYKSVNIIF	111	353
HLA-A*02:11	KSVNITFEL	KSVNIIFEL	169	55
HLA-C*14:02	GYKSVNITF	GYKSVNIIF	53	160
HLA-A*11:01	ITFELDERIDK	IIFELDERIDK	446	1259
HLA-A*02:01	KSVNITFEL	KSVNIIFEL	863	307
HLA-C*03:02	YKSVNITFEL	YKSVNIIFEL	299	784
HLA-A*68:01	VNITFELDER	VNIIFELDER	1032	394
HLA-A*02:06	KSVNITFEL	KSVNIIFEL	80	31
HLA-C*16:01	YKSVNITFEL	YKSVNIIFEL	310	793
HLA-A*68:02	NITFELDERI	NIIFELDERI	209	533
HLA-A*24:02	GYKSVNITF	GYKSVNIIF	83	192
HLA-A*02:11	ITFELDERIDKV	IIFELDERIDKV	397	175
HLA-A*68:01	SVNITFELDER	SVNIIFELDER	405	180
HLA-A*24:02	QGYKSVNITF	QGYKSVNIIF	208	455
HLA-A*02:06	ITFELDERI	IIFELDERI	402	185
HLA-A*02:11	YKSVNITFEL	YKSVNIIFEL	715	337
HLA-C*03:03	KSVNITFEL	KSVNIIFEL	115	233
HLA-C*03:04	KSVNITFEL	KSVNIIFEL	115	233

G489S

Reference  DAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQ
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    DAPYIVGDVVQEGVLTAVVIPTKKASGTTEMLAKALRKVPTDNYITTYPGQ

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*11:01	AGGTTEMLAK	ASGTTEMLAK	1254	37
HLA-A*03:01	AGGTTEMLAK	ASGTTEMLAK	3665	277
HLA-A*03:02	AGGTTEMLAK	ASGTTEMLAK	2689	228
HLA-C*03:03	KAGGTTEML	KASGTTEML	2418	212
HLA-C*03:04	KAGGTTEML	KASGTTEML	2418	212
HLA-C*16:01	KAGGTTEML	KASGTTEML	1012	116
HLA-C*17:01	KAGGTTEML	KASGTTEML	1802	287
HLA-A*31:01	AGGTTEMLAKALR	ASGTTEMLAKALR	787	133
HLA-B*58:01	KAGGTTEML	KASGTTEML	2117	440
HLA-C*16:01	KAGGTTEM	KASGTTEM	863	191
HLA-A*03:02	GGTTEMLAKALRK	SGTTEMLAKALRK	1693	453
HLA-A*11:01	KAGGTTEMLAK	KASGTTEMLAK	589	203
HLA-A*03:01	KAGGTTEMLAK	KASGTTEMLAK	1236	449
HLA-A*03:01	GGTTEMLAKALRK	SGTTEMLAKALRK	467	181
HLA-A*31:01	GGTTEMLAKALR	SGTTEMLAKALR	677	335

NSP4 protein

L264F

Reference  GRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGI
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    GRWVLNNDYYRSLPGVFCGVDAVNLFTNMFTPLIQPIGALDISASIVAGGI

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*02:11	LLTNMFTPL	LFTNMFTPL	5	2700
HLA-A*02:01	LLTNMFTPL	LFTNMFTPL	16	6477
HLA-A*02:02	LLTNMFTPL	LFTNMFTPL	5	1518
HLA-A*02:06	LLTNMFTPL	LFTNMFTPL	28	3590
HLA-A*02:11	LLTNMFTPLI	LFTNMFTPLI	17	1231
HLA-A*02:01	LLTNMFTPLI	LFTNMFTPLI	55	3749
HLA-C*14:02	LLTNMFTPL	LFTNMFTPL	453	7
HLA-A*02:02	LLTNMFTPLI	LFTNMFTPLI	22	967
HLA-B*15:01	LLTNMFTPL	LFTNMFTPL	228	5949
HLA-C*03:03	LTNMFTPL	FTNMFTPL	1113	61
HLA-C*03:04	LTNMFTPL	FTNMFTPL	1113	61
HLA-A*23:01	VFCGVDAVNLL	VFCGVDAVNLF	2671	151
HLA-A*02:02	LLTNMFTPLIQPI	LFTNMFTPLIQPI	294	4718
HLA-A*02:02	GVFCGVDAVNLL	GVFCGVDAVNLF	439	6440
HLA-C*14:02	NLLTNMFTPL	NLFTNMFTPL	4861	337
HLA-B*15:03	CGVDAVNLL	CGVDAVNLF	6087	490
HLA-A*02:11	LLTNMFTPLIQPI	LFTNMFTPLIQPI	297	3332
HLA-C*03:02	LTNMFTPL	FTNMFTPL	829	77
HLA-C*03:03	LTNMFTPLI	FTNMFTPLI	2207	207
HLA-C*03:04	LTNMFTPLI	FTNMFTPLI	2207	207
HLA-A*02:01	LTNMFTPLI	FTNMFTPLI	1097	103
HLA-A*02:11	LTNMFTPLI	FTNMFTPLI	136	13
HLA-C*12:03	LTNMFTPLI	FTNMFTPLI	430	42
HLA-A*02:06	LLTNMFTPLI	LFTNMFTPLI	136	1345
HLA-A*02:06	LTNMFTPLI	FTNMFTPLI	223	23
HLA-C*12:02	LTNMFTPLI	FTNMFTPLI	1404	157
HLA-A*02:02	LTNMFTPLI	FTNMFTPLI	364	41
HLA-A*68:02	LTNMFTPLIQPI	FTNMFTPLIQPI	1086	129
HLA-C*12:03	LTNMFTPL	FTNMFTPL	2677	324
HLA-C*03:02	LTNMFTPLI	FTNMFTPLI	716	87
HLA-C*02:02	LTNMFTPLI	FTNMFTPLI	1268	158
HLA-C*02:10	LTNMFTPLI	FTNMFTPLI	1268	158
HLA-C*17:01	LTNMFTPLI	FTNMFTPLI	599	86
HLA-A*68:02	LTNMFTPLI	FTNMFTPLI	82	12
HLA-C*03:03	LLTNMFTPL	LFTNMFTPL	253	1728
HLA-C*03:04	LLTNMFTPL	LFTNMFTPL	253	1728
HLA-C*12:02	LTNMFTPL	FTNMFTPL	3002	450
HLA-B*15:03	LLTNMFTPL	LFTNMFTPL	108	704
HLA-A*68:02	NLLTNMFTPL	NLFTNMFTPL	2741	480
HLA-C*14:02	LTNMFTPL	FTNMFTPL	2589	467
HLA-A*02:06	LTNMFTPLIQPI	FTNMFTPLIQPI	2171	394
HLA-C*16:01	LTNMFTPL	FTNMFTPL	293	61
HLA-C*16:01	LTNMFTPLI	FTNMFTPLI	267	56
HLA-A*30:01	LTNMFTPLI	FTNMFTPLI	144	648
HLA-A*02:02	AVNLLTNMFTPL	AVNLFTNMFTPL	249	948
HLA-B*08:01	LLTNMFTPL	LFTNMFTPL	271	983
HLA-A*02:11	LTNMFTPLIQPI	FTNMFTPLIQPI	1413	409
HLA-C*03:02	LLTNMFTPL	LFTNMFTPL	307	951
HLA-A*02:02	NLLTNMFTPLI	NLFTNMFTPLI	658	224
HLA-A*02:02	NLLTNMFTPL	NLFTNMFTPL	58	21
HLA-C*15:02	LTNMFTPLI	FTNMFTPLI	67	25
HLA-A*02:11	AVNLLTNMFTPL	AVNLFTNMFTPL	390	1009
HLA-A*02:02	VNLLTNMFTPL	VNLFTNMFTPL	375	919
HLA-A*02:11	NLLTNMFTP	NLFTNMFTP	1166	476
HLA-B*15:03	AVNLLTNMF	AVNLFTNMF	390	172
HLA-A*02:11	NLLTNMFTPLIQPI	NLFTNMFTPLIQPI	274	129

T327I

Reference  MRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFY
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    MRFRRAFGEYSHVVAFNTLLFLMSFIVLCLTPVYSFLPGVYSVIYLYLTFY

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*02:11	NTLLFLMSFT	NTLLFLMSFI	1059	51
HLA-A*68:02	NTLLFLMSFT	NTLLFLMSFI	741	41
HLA-A*02:11	FNTLLFLMSFT	FNTLLFLMSFI	1509	110
HLA-A*02:11	TLLFLMSFT	TLLFLMSFI	82	6
HLA-A*68:02	TLLFLMSFT	TLLFLMSFI	3858	286
HLA-A*68:02	FTVLCLTPVYSFL	FIVLCLTPVYSFL	129	1580
HLA-A*02:11	AFNTLLFLMSFT	AFNTLLFLMSFI	4598	420
HLA-A*68:02	FTVLCLTPV	FIVLCLTPV	8	83
HLA-A*68:02	SFTVLCLTPV	SFIVLCLTPV	63	597
HLA-A*02:01	TLLFLMSFT	TLLFLMSFI	239	26
HLA-A*02:01	NTLLFLMSFT	NTLLFLMSFI	1593	179
HLA-A*02:02	TLLFLMSFT	TLLFLMSFI	235	28
HLA-B*15:01	LLFLMSFTVL	LLFLMSFIVL	229	1839
HLA-A*02:11	LFLMSFTV	LFLMSFIV	279	2222
HLA-A*02:02	NTLLFLMSFT	NTLLFLMSFI	1462	186
HLA-A*02:11	LLFLMSFT	LLFLMSFI	2935	377
HLA-A*32:01	LLFLMSFTV	LLFLMSFIV	369	2631
HLA-A*02:06	LLFLMSFTV	LLFLMSFIV	34	231
HLA-C*12:03	FTVLCLTPV	FIVLCLTPV	161	1041
HLA-C*14:02	LFLMSFTVL	LFLMSFIVL	18	112
HLA-A*02:11	AFNTLLFLMSFTV	AFNTLLFLMSFIV	74	448
HLA-C*16:01	FTVLCLTPVY	FIVLCLTPVY	395	2359
HLA-A*02:06	TLLFLMSFT	TLLFLMSFI	397	68
HLA-A*02:01	LLFLMSFTV	LLFLMSFIV	9	50
HLA-A*02:11	VAFNTLLFLMSFTV	VAFNTLLFLMSFIV	225	1206
HLA-A*02:01	AFNTLLFLMSFTV	AFNTLLFLMSFIV	357	1842
HLA-A*68:02	NTLLFLMSFTV	NTLLFLMSFIV	106	539
HLA-A*02:11	NTLLFLMSFTV	NTLLFLMSFIV	59	300
HLA-A*02:01	NTLLFLMSFTV	NTLLFLMSFIV	212	1074
HLA-C*03:03	FTVLCLTPV	FIVLCLTPV	302	1526
HLA-C*03:04	FTVLCLTPV	FIVLCLTPV	302	1526
HLA-B*15:03	LLFLMSFTVL	LLFLMSFIVL	318	1563
HLA-A*02:02	LLFLMSFTV	LLFLMSFIV	11	53
HLA-A*02:06	NTLLFLMSFT	NTLLFLMSFI	945	197
HLA-A*02:11	SFTVLCLTPV	SFIVLCLTPV	250	53
HLA-A*02:11	FNTLLFLMSFTV	FNTLLFLMSFIV	23	108
HLA-A*02:01	FNTLLFLMSFTV	FNTLLFLMSFIV	131	602
HLA-C*03:02	FTVLCLTPV	FIVLCLTPV	198	902
HLA-C*14:02	FLMSFTVL	FLMSFIVL	475	2138
HLA-C*12:02	FTVLCLTPV	FIVLCLTPV	218	961
HLA-C*03:03	FLMSFTVL	FLMSFIVL	309	1359
HLA-C*03:04	FLMSFTVL	FLMSFIVL	309	1359
HLA-A*26:01	FTVLCLTPVY	FIVLCLTPVY	176	760
HLA-A*02:01	SFTVLCLTPV	SFIVLCLTPV	470	111
HLA-B*15:01	FLMSFTVLCL	FLMSFIVLCL	455	1914
HLA-B*15:03	LFLMSFTVL	LFLMSFIVL	353	1462
HLA-A*68:01	FTVLCLTPVY	FIVLCLTPVY	485	1972
HLA-A*02:02	FNTLLFLMSFTV	FNTLLFLMSFIV	186	704
HLA-B*15:01	LMSFTVLCL	LMSFIVLCL	110	408
HLA-C*03:02	FLMSFTVL	FLMSFIVL	352	1285
HLA-A*02:02	NTLLFLMSFTV	NTLLFLMSFIV	424	1515
HLA-A*02:11	LLFLMSFTV	LLFLMSFIV	2	7
HLA-C*03:02	FTVLCLTPVY	FIVLCLTPVY	50	175
HLA-B*15:03	LMSFTVLCL	LMSFIVLCL	54	186
HLA-C*12:02	FTVLCLTPVY	FIVLCLTPVY	331	1137
HLA-A*02:02	LLFLMSFTVL	LLFLMSFIVL	42	144
HLA-A*68:02	MSFTVLCLTPV	MSFIVLCLTPV	34	114
HLA-C*03:02	LMSFTVLCL	LMSFIVLCL	467	1520
HLA-A*02:01	LLFLMSFTVL	LLFLMSFIVL	74	240
HLA-A*02:11	LLFLMSFTVL	LLFLMSFIVL	25	80
HLA-A*02:02	SFTVLCLTPV	SFIVLCLTPV	261	84
HLA-A*02:02	LFLMSFTVLCL	LFLMSFIVLCL	73	216
HLA-A*02:06	LLFLMSFTVL	LLFLMSFIVL	331	973
HLA-C*16:01	MSFTVLCL	MSFIVLCL	171	496
HLA-A*68:02	FLMSFTVLCLTPV	FLMSFIVLCLTPV	331	927
HLA-A*68:02	MSFTVLCLT	MSFIVLCLT	68	186
HLA-A*02:06	MSFTVLCLT	MSFIVLCLT	239	650
HLA-A*02:11	TVLCLTPVYSFL	IVLCLTPVYSFL	533	199
HLA-B*15:03	FLMSFTVL	FLMSFIVL	371	993
HLA-A*02:02	FLMSFTVLCL	FLMSFIVLCL	3	8
HLA-A*02:02	LLFLMSFTVLCL	LLFLMSFIVLCL	134	356
HLA-A*02:01	LLFLMSFTVLCL	LLFLMSFIVLCL	237	602
HLA-A*02:01	LFLMSFTVLCL	LFLMSFIVLCL	73	185
HLA-A*02:06	FLMSFTVLCL	FLMSFIVLCL	15	38
HLA-C*03:02	TVLCLTPVY	IVLCLTPVY	136	54
HLA-A*23:01	LFLMSFTVL	LFLMSFIVL	411	1026
HLA-A*02:06	TLLFLMSFTV	TLLFLMSFIV	47	116
HLA-B*08:01	FLMSFTVL	FLMSFIVL	118	291
HLA-A*02:06	NTLLFLMSFTV	NTLLFLMSFIV	373	906
HLA-A*02:01	TLLFLMSFTV	TLLFLMSFIV	14	33
HLA-A*02:06	VAFNTLLFLMSFTV	VAFNTLLFLMSFIV	479	1101
HLA-A*02:06	MSFTVLCLTPV	MSFIVLCLTPV	48	110
HLA-B*58:01	MSFTVLCLT	MSFIVLCLT	412	943
HLA-A*02:01	FLMSFTVLCL	FLMSFIVLCL	4	9
HLA-A*02:02	FLMSFTVLCLTP	FLMSFIVLCLTP	194	430
HLA-A*02:11	TLLFLMSFTV	TLLFLMSFIV	5	11
HLA-A*02:02	FTVLCLTPV	FIVLCLTPV	23	11
HLA-A*02:01	FTVLCLTPV	FIVLCLTPV	25	12
HLA-A*30:02	FTVLCLTPVY	FIVLCLTPVY	327	671
HLA-A*02:02	TVLCLTPVYSFL	IVLCLTPVYSFL	176	86
HLA-B*58:01	MSFTVLCLTP	MSFIVLCLTP	497	1015
HLA-A*02:11	FTVLCLTPV	FIVLCLTPV	12	6

T492I

Reference  ACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQ
           ||||||||||||||||||||||||| ||||||||
Mutated    ACCHLAKALNDFSNSGSDVLYQPPQISITSAVLQ

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-C*16:01	QTSITSAVL	QISITSAVL	201	1829
HLA-A*68:02	QTSITSAVL	QISITSAVL	206	1702
HLA-A*02:06	QPPQTSITSAV	QPPQISITSAV	3097	393
HLA-A*02:06	LYQPPQTSITSAV	LYQPPQISITSAV	1485	214
HLA-A*02:06	YQPPQTSITSAV	YQPPQISITSAV	156	26
HLA-A*02:01	YQPPQTSITSAV	YQPPQISITSAV	1613	341
HLA-A*02:11	YQPPQTSITSAV	YQPPQISITSAV	498	120
HLA-C*03:03	TSITSAVL	ISITSAVL	532	198
HLA-C*03:04	TSITSAVL	ISITSAVL	532	198
HLA-C*03:03	QTSITSAVL	QISITSAVL	311	831
HLA-C*03:04	QTSITSAVL	QISITSAVL	311	831
HLA-C*16:01	TSITSAVL	ISITSAVL	381	156
HLA-A*02:01	VLYQPPQTSI	VLYQPPQISI	1084	474
HLA-A*23:01	VLYQPPQTSI	VLYQPPQISI	979	462
HLA-A*23:01	LYQPPQTSI	LYQPPQISI	278	132

NSP5 protein

P132H

Reference  QPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCV
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    QPGQTFSVLACYNGSPSGVYQCAMRHNFTIKGSFLNGSCGSVGFNIDYDCV

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*07:02	RPNFTIKGSF	RHNFTIKGSF	14	15756
HLA-B*07:02	RPNFTIKGSFL	RHNFTIKGSFL	24	18650
HLA-B*42:01	RPNFTIKGSF	RHNFTIKGSF	31	15129
HLA-B*42:01	RPNFTIKGSFL	RHNFTIKGSFL	39	17143
HLA-B*15:03	PNFTIKGSF	HNFTIKGSF	7728	117
HLA-A*03:01	GVYQCAMRP	GVYQCAMRH	20799	417
HLA-B*08:01	CAMRPNFTI	CAMRHNFTI	3487	242
HLA-B*27:05	MRPNFTIKG	MRHNFTIKG	4762	366
HLA-B*27:05	MRPNFTIKGSFL	MRHNFTIKGSFL	827	86
HLA-B*15:03	RPNFTIKGSF	RHNFTIKGSF	432	54
HLA-B*27:05	MRPNFTIK	MRHNFTIK	2132	276
HLA-B*15:03	MRPNFTIKGSF	MRHNFTIKGSF	397	96
HLA-C*07:02	MRPNFTIKGSF	MRHNFTIKGSF	361	1054
HLA-B*27:05	MRPNFTIKGSF	MRHNFTIKGSF	218	78
HLA-A*30:01	QCAMRPNFTIK	QCAMRHNFTIK	199	513
HLA-A*68:02	CAMRPNFTI	CAMRHNFTI	199	493
HLA-C*16:01	CAMRPNFTI	CAMRHNFTI	125	52
HLA-A*30:01	AMRPNFTIKG	AMRHNFTIKG	123	279
HLA-C*12:03	CAMRPNFTI	CAMRHNFTI	317	143
HLA-A*30:01	VYQCAMRPNFTIK	VYQCAMRHNFTIK	316	683
HLA-A*11:01	AMRPNFTIK	AMRHNFTIK	175	376
HLA-A*11:01	CAMRPNFTIK	CAMRHNFTIK	42	88
HLA-A*68:01	CAMRPNFTIK	CAMRHNFTIK	127	254

NSP6 protein

F108L

Reference  MVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYD
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    MVYMPASWVMRIMTWLDMVDTSLSGLKLKDCVMYASAVVLLILMTARTVYD

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*02:11	MVDTSLSGF	MVDTSLSGL	4111	89
HLA-A*02:02	MVDTSLSGF	MVDTSLSGL	4757	170
HLA-A*02:11	WLDMVDTSLSGF	WLDMVDTSLSGL	6135	222
HLA-A*02:02	WLDMVDTSLSGF	WLDMVDTSLSGL	4418	169
HLA-B*15:01	GFKLKDCVMY	GLKLKDCVMY	7051	330
HLA-A*68:02	MVDTSLSGF	MVDTSLSGL	9003	422
HLA-B*15:01	GFKLKDCVM	GLKLKDCVM	9405	463
HLA-B*15:01	DMVDTSLSGF	DMVDTSLSGL	362	5850
HLA-A*02:06	MVDTSLSGF	MVDTSLSGL	1359	89
HLA-B*15:03	MVDTSLSGF	MVDTSLSGL	276	4185
HLA-B*35:01	MVDTSLSGF	MVDTSLSGL	490	6822
HLA-A*26:01	DMVDTSLSGF	DMVDTSLSGL	417	4499
HLA-A*23:01	TWLDMVDTSLSGF	TWLDMVDTSLSGL	387	4048
HLA-A*02:06	FKLKDCVMYA	LKLKDCVMYA	90	586
HLA-A*02:01	FKLKDCVMYA	LKLKDCVMYA	27	165
HLA-C*17:01	MVDTSLSGF	MVDTSLSGL	1293	215
HLA-B*08:01	GFKLKDCVM	GLKLKDCVM	1477	282
HLA-A*68:02	DTSLSGFKL	DTSLSGLKL	313	1612
HLA-C*03:02	MVDTSLSGF	MVDTSLSGL	211	952
HLA-A*02:11	FKLKDCVMYA	LKLKDCVMYA	12	54
HLA-B*08:01	SGFKLKDCVM	SGLKLKDCVM	920	237
HLA-A*02:02	FKLKDCVMYA	LKLKDCVMYA	8	28
HLA-C*08:02	MVDTSLSGF	MVDTSLSGL	159	47
HLA-A*68:01	DTSLSGFKLK	DTSLSGLKLK	33	101
HLA-C*03:03	MVDTSLSGF	MVDTSLSGL	1448	478
HLA-C*03:04	MVDTSLSGF	MVDTSLSGL	1448	478
HLA-A*02:11	SLSGFKLKDCV	SLSGLKLKDCV	122	354
HLA-A*02:11	FKLKDCVMYASAVV	LKLKDCVMYASAVV	481	1324
HLA-A*30:01	TSLSGFKLK	TSLSGLKLK	107	266
HLA-A*02:02	SLSGFKLKDCV	SLSGLKLKDCV	408	853

NSP12 protein

P323L

Reference  CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*02:01	FPPTSFGPLV	FPLTSFGPLV	8999	21
HLA-A*02:02	FPPTSFGPLV	FPLTSFGPLV	7237	18
HLA-A*02:11	FPPTSFGPLV	FPLTSFGPLV	3405	9
HLA-A*32:01	VLFSTVFPP	VLFSTVFPL	5415	27
HLA-A*02:11	LFSTVFPP	LFSTVFPL	6706	35
HLA-A*02:11	ANFNVLFSTVFPP	ANFNVLFSTVFPL	1827	12
HLA-A*02:11	NFNVLFSTVFPP	NFNVLFSTVFPL	1779	13
HLA-A*02:11	NVLFSTVFPP	NVLFSTVFPL	739	6
HLA-C*03:03	VLFSTVFPP	VLFSTVFPL	28468	265
HLA-C*03:04	VLFSTVFPP	VLFSTVFPL	28468	265
HLA-A*02:11	FNVLFSTVFPP	FNVLFSTVFPL	413	4
HLA-C*14:02	VLFSTVFPP	VLFSTVFPL	21407	220
HLA-A*02:11	FNVLFSTVFPPT	FNVLFSTVFPLT	2618	27
HLA-A*02:01	LFSTVFPP	LFSTVFPL	7475	81
HLA-C*14:02	LFSTVFPP	LFSTVFPL	11979	138
HLA-B*15:03	VLFSTVFPP	VLFSTVFPL	3115	36
HLA-A*02:11	VLFSTVFPP	VLFSTVFPL	78	1
HLA-C*03:02	VLFSTVFPP	VLFSTVFPL	20533	273
HLA-A*02:01	FNVLFSTVFPP	FNVLFSTVFPL	656	9
HLA-A*02:01	ANFNVLFSTVFPP	ANFNVLFSTVFPL	2569	37
HLA-A*02:11	FNVLFSTVFPPTS	FNVLFSTVFPLTS	9448	142
HLA-A*02:02	NVLFSTVFPP	NVLFSTVFPL	1088	17
HLA-A*02:11	CANFNVLFSTVFPP	CANFNVLFSTVFPL	6143	96
HLA-A*02:02	FNVLFSTVFPP	FNVLFSTVFPL	1014	16
HLA-A*68:02	NVLFSTVFPP	NVLFSTVFPL	2213	37
HLA-A*02:02	ANFNVLFSTVFPP	ANFNVLFSTVFPL	3126	53
HLA-A*02:11	PPTSFGPLV	PLTSFGPLV	28329	483
HLA-C*16:01	VLFSTVFPP	VLFSTVFPL	24733	432
HLA-C*17:01	VLFSTVFPP	VLFSTVFPL	25087	439
HLA-A*02:01	FNVLFSTVFPPT	FNVLFSTVFPLT	3765	66
HLA-A*02:02	LFSTVFPP	LFSTVFPL	9311	167
HLA-A*02:01	NVLFSTVFPP	NVLFSTVFPL	928	17
HLA-A*02:02	NFNVLFSTVFPP	NFNVLFSTVFPL	2542	47
HLA-A*02:02	VLFSTVFPP	VLFSTVFPL	269	5
HLA-A*02:01	NFNVLFSTVFPP	NFNVLFSTVFPL	2286	44
HLA-A*02:06	FPPTSFGPLV	FPLTSFGPLV	3379	73
HLA-A*02:01	VLFSTVFPP	VLFSTVFPL	179	4
HLA-A*02:06	FNVLFSTVFPP	FNVLFSTVFPL	1609	37
HLA-A*02:02	FNVLFSTVFPPT	FNVLFSTVFPLT	4116	110
HLA-A*02:11	FSTVFPPTSFGPLV	FSTVFPLTSFGPLV	6695	180
HLA-A*02:01	FNVLFSTVFPPTS	FNVLFSTVFPLTS	11451	311
HLA-A*02:06	VLFSTVFPP	VLFSTVFPL	368	10
HLA-A*02:11	ANFNVLFSTVFPPT	ANFNVLFSTVFPLT	14639	409
HLA-A*02:01	FSTVFPPTSFGPLV	FSTVFPLTSFGPLV	12596	425
HLA-A*68:01	PTSFGPLVR	LTSFGPLVR	908	36
HLA-A*02:06	NVLFSTVFPP	NVLFSTVFPL	1611	66
HLA-B*15:01	VLFSTVFPP	VLFSTVFPL	5731	243
HLA-B*35:01	FPPTSFGPL	FPLTSFGPL	304	13
HLA-B*53:01	FPPTSFGPL	FPLTSFGPL	3581	154
HLA-C*01:02	FPPTSFGPL	FPLTSFGPL	217	4855
HLA-A*02:01	CANFNVLFSTVFPP	CANFNVLFSTVFPL	8519	384
HLA-A*02:02	FNVLFSTVFPPTS	FNVLFSTVFPLTS	10920	497
HLA-A*02:02	FSTVFPPTSFGPLV	FSTVFPLTSFGPLV	6958	322
HLA-A*02:11	VFPPTSFGPLV	VFPLTSFGPLV	4242	199
HLA-A*02:02	CANFNVLFSTVFPP	CANFNVLFSTVFPL	8183	391
HLA-A*02:11	NFNVLFSTVFPPT	NFNVLFSTVFPLT	9111	472
HLA-A*02:06	ANFNVLFSTVFPP	ANFNVLFSTVFPL	5191	304
HLA-A*02:06	NFNVLFSTVFPP	NFNVLFSTVFPL	4220	274
HLA-A*02:06	FNVLFSTVFPPT	FNVLFSTVFPLT	4868	388
HLA-B*35:03	FPPTSFGPL	FPLTSFGPL	286	24
HLA-A*03:01	PTSFGPLVRK	LTSFGPLVRK	554	47
HLA-A*31:01	PTSFGPLVR	LTSFGPLVR	4402	390
HLA-A*11:01	PTSFGPLVR	LTSFGPLVR	4068	379
HLA-A*03:02	PTSFGPLVRK	LTSFGPLVRK	868	88
HLA-B*07:02	STVFPPTSFGPL	STVFPLTSFGPL	777	79
HLA-A*68:01	PTSFGPLVRK	LTSFGPLVRK	308	34
HLA-B*07:02	TVFPPTSFGPL	TVFPLTSFGPL	1498	171
HLA-A*02:11	STVFPPTSFGPLV	STVFPLTSFGPLV	2427	285
HLA-A*02:11	NVLFSTVFPPT	NVLFSTVFPLT	1183	139
HLA-A*33:03	PTSFGPLVR	LTSFGPLVR	3228	381
HLA-A*26:01	STVFPPTSF	STVFPLTSF	786	95
HLA-B*07:02	VFPPTSFGPL	VFPLTSFGPL	339	43
HLA-A*02:01	NVLFSTVFPPT	NVLFSTVFPLT	2111	290
HLA-B*42:01	FSTVFPPTSFGPL	FSTVFPLTSFGPL	1320	185
HLA-B*42:01	STVFPPTSFGPL	STVFPLTSFGPL	738	111
HLA-A*02:01	VLFSTVFPPTSF	VLFSTVFPLTSF	2225	342
HLA-B*08:01	FPPTSFGPL	FPLTSFGPL	973	153
HLA-B*42:01	TVFPPTSFGPL	TVFPLTSFGPL	843	142
HLA-A*11:01	PTSFGPLVRK	LTSFGPLVRK	123	21
HLA-B*42:01	VFPPTSFGPL	VFPLTSFGPL	274	47
HLA-A*02:01	VLFSTVFPPTS	VLFSTVFPLTS	708	124
HLA-B*07:02	FSTVFPPTSFGPL	FSTVFPLTSFGPL	2537	466
HLA-B*07:02	FPPTSFGPL	FPLTSFGPL	42	8
HLA-A*02:11	VLFSTVFPPTSF	VLFSTVFPLTSF	1019	195
HLA-A*25:01	STVFPPTSF	STVFPLTSF	1533	296
HLA-B*07:02	FPPTSFGPLV	FPLTSFGPLV	1131	236
HLA-B*42:01	FPPTSFGPL	FPLTSFGPL	21	5
HLA-A*26:01	FSTVFPPTSF	FSTVFPLTSF	1667	398
HLA-B*51:01	FPPTSFGPL	FPLTSFGPL	1809	433
HLA-A*02:11	VLFSTVFPPTS	VLFSTVFPLTS	309	74
HLA-B*42:01	FPPTSFGPLV	FPLTSFGPLV	368	89
HLA-A*02:02	VLFSTVFPPTS	VLFSTVFPLTS	1210	377
HLA-C*03:02	FPPTSFGPL	FPLTSFGPL	960	313
HLA-A*32:01	STVFPPTSF	STVFPLTSF	230	86
HLA-B*58:01	FSTVFPPTSF	FSTVFPLTSF	88	33
HLA-B*15:01	STVFPPTSF	STVFPLTSF	130	49
HLA-A*02:01	VLFSTVFPPT	VLFSTVFPLT	61	23
HLA-C*03:03	FPPTSFGPL	FPLTSFGPL	375	145
HLA-C*03:04	FPPTSFGPL	FPLTSFGPL	375	145
HLA-A*02:02	VLFSTVFPPT	VLFSTVFPLT	113	44
HLA-C*16:01	FSTVFPPTS	FSTVFPLTS	1127	460
HLA-C*14:02	LFSTVFPPTSF	LFSTVFPLTSF	396	950
HLA-C*01:02	VFPPTSFGPL	VFPLTSFGPL	429	997
HLA-A*02:02	TVFPPTSFGPLV	TVFPLTSFGPLV	789	343
HLA-A*68:02	STVFPPTSFGPLV	STVFPLTSFGPLV	169	74
HLA-A*02:11	VLFSTVFPPT	VLFSTVFPLT	25	11
HLA-A*03:01	TVFPPTSFGPLVRK	TVFPLTSFGPLVRK	92	198
HLA-C*14:02	VFPPTSFGPL	VFPLTSFGPL	47	99
HLA-A*23:01	LFSTVFPPTSF	LFSTVFPLTSF	370	179

NSP13 protein

R392C

Reference  TADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTL
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    TADIVVFDEISMATNYDLSVVNARLCAKHYVYIGDPAQLPAPRTLLTKGTL

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*68:01	DLSVVNARLR	DLSVVNARLC	44	30873
HLA-C*06:02	LRAKHYVYI	LCAKHYVYI	36	16159
HLA-A*68:01	LSVVNARLR	LSVVNARLC	140	32767
HLA-A*33:03	DLSVVNARLR	DLSVVNARLC	154	32767
HLA-A*31:01	LSVVNARLR	LSVVNARLC	261	30993
HLA-C*07:01	LRAKHYVYI	LCAKHYVYI	133	15165
HLA-B*27:05	LRAKHYVYI	LCAKHYVYI	379	32767
HLA-A*30:01	RLRAKHYVY	RLCAKHYVY	27	1483
HLA-B*27:05	RLRAKHYVYI	RLCAKHYVYI	208	10096
HLA-C*07:02	LRAKHYVYI	LCAKHYVYI	433	17614
HLA-A*30:01	RAKHYVYIG	CAKHYVYIG	76	2630
HLA-A*68:01	TNYDLSVVNARLR	TNYDLSVVNARLC	477	15989
HLA-A*30:01	RLRAKHYV	RLCAKHYV	218	5721
HLA-A*30:01	RLRAKHYVYI	RLCAKHYVYI	17	427
HLA-A*30:01	RAKHYVYIGDPA	CAKHYVYIGDPA	188	4601
HLA-A*30:01	RAKHYVYI	CAKHYVYI	211	4134
HLA-B*08:01	RLRAKHYV	RLCAKHYV	363	6671
HLA-A*30:01	ARLRAKHYVYI	ARLCAKHYVYI	422	6081
HLA-B*08:01	RLRAKHYVYI	RLCAKHYVYI	230	2547
HLA-B*15:03	LRAKHYVY	LCAKHYVY	395	4119
HLA-A*29:02	RLRAKHYVY	RLCAKHYVY	535	101
HLA-A*02:11	RLRAKHYV	RLCAKHYV	1483	352
HLA-A*68:01	VVNARLRAK	VVNARLCAK	1428	381
HLA-A*11:01	VVNARLRAK	VVNARLCAK	145	44
HLA-A*03:02	VVNARLRAK	VVNARLCAK	257	79
HLA-B*15:01	ARLRAKHYVY	ARLCAKHYVY	402	1223
HLA-B*15:01	RLRAKHYVY	RLCAKHYVY	49	133
HLA-A*68:01	SVVNARLRAK	SVVNARLCAK	158	63
HLA-A*03:02	SVVNARLRAK	SVVNARLCAK	196	86

T481M

Reference  VYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAW
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    VYDNKLKAHKDKSAQCFKMFYKGVIMHDVSSAINRPQIGVVREFLTRNPAW

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-B*15:03	FKMFYKGVIT	FKMFYKGVIM	2559	18
HLA-C*14:02	MFYKGVIT	MFYKGVIM	3030	23
HLA-A*32:01	KMFYKGVIT	KMFYKGVIM	5348	42
HLA-B*15:01	KMFYKGVIT	KMFYKGVIM	3740	34
HLA-A*02:11	ITHDVSSAI	IMHDVSSAI	510	5
HLA-B*15:03	KMFYKGVIT	KMFYKGVIM	366	4
HLA-A*02:01	ITHDVSSAI	IMHDVSSAI	5544	63
HLA-A*02:02	ITHDVSSAI	IMHDVSSAI	591	8
HLA-A*02:11	YKGVITHDVSSAI	YKGVIMHDVSSAI	6591	94
HLA-B*15:01	FKMFYKGVIT	FKMFYKGVIM	7290	118
HLA-C*03:02	KMFYKGVIT	KMFYKGVIM	12717	219
HLA-C*14:02	KMFYKGVIT	KMFYKGVIM	9015	161
HLA-A*02:02	YKGVITHDVSSAI	YKGVIMHDVSSAI	3265	62
HLA-C*03:02	FKMFYKGVIT	FKMFYKGVIM	14570	282
HLA-A*02:11	VITHDVSSA	VIMHDVSSA	3858	76
HLA-A*68:02	ITHDVSSAI	IMHDVSSAI	46	2218
HLA-B*15:03	ITHDVSSAI	IMHDVSSAI	819	17
HLA-B*15:03	AQCFKMFYKGVIT	AQCFKMFYKGVIM	13026	280
HLA-C*16:01	KMFYKGVIT	KMFYKGVIM	11102	239
HLA-A*02:02	FYKGVITHDVSSAI	FYKGVIMHDVSSAI	4510	105
HLA-A*02:11	FYKGVITHDVSSAI	FYKGVIMHDVSSAI	8883	225
HLA-A*02:11	KGVITHDVSSAI	KGVIMHDVSSAI	11207	293
HLA-A*68:02	YKGVITHDVSSAI	YKGVIMHDVSSAI	261	8680
HLA-A*02:06	VITHDVSSA	VIMHDVSSA	3344	112
HLA-A*02:11	YKGVITHDVSSA	YKGVIMHDVSSA	13228	476
HLA-A*02:11	VITHDVSSAI	VIMHDVSSAI	1875	69
HLA-A*02:02	ITHDVSSAIN	IMHDVSSAIN	12028	482
HLA-C*12:03	KMFYKGVIT	KMFYKGVIM	10576	438
HLA-A*02:02	KGVITHDVSSAI	KGVIMHDVSSAI	6748	316
HLA-B*15:01	ITHDVSSAI	IMHDVSSAI	1472	70
HLA-C*15:02	ITHDVSSAI	IMHDVSSAI	134	2740
HLA-A*68:01	ITHDVSSAINR	IMHDVSSAINR	87	1539
HLA-A*02:02	VITHDVSSA	VIMHDVSSA	3007	205
HLA-A*02:02	YKGVITHDVSSA	YKGVIMHDVSSA	6712	498
HLA-A*02:06	VITHDVSSAI	VIMHDVSSAI	3355	268
HLA-A*02:02	FYKGVITHDVSSA	FYKGVIMHDVSSA	6020	495
HLA-A*02:01	VITHDVSSAI	VIMHDVSSAI	5432	465
HLA-A*02:02	VITHDVSSAI	VIMHDVSSAI	632	73
HLA-B*15:01	VITHDVSSAI	VIMHDVSSAI	3267	379
HLA-C*14:02	ITHDVSSAI	IMHDVSSAI	1082	149
HLA-A*02:06	ITHDVSSAI	IMHDVSSAI	846	185
HLA-C*14:02	MFYKGVITH	MFYKGVIMH	110	477
HLA-A*31:01	VITHDVSSAINR	VIMHDVSSAINR	1939	452
HLA-C*16:01	ITHDVSSAI	IMHDVSSAI	69	285
HLA-B*38:01	THDVSSAI	MHDVSSAI	1298	317
HLA-B*15:03	MFYKGVITH	MFYKGVIMH	333	1338
HLA-A*02:11	KMFYKGVIT	KMFYKGVIM	201	59
HLA-A*32:01	ITHDVSSAI	IMHDVSSAI	1049	323
HLA-A*02:02	KMFYKGVIT	KMFYKGVIM	821	290
HLA-C*12:03	ITHDVSSAI	IMHDVSSAI	29	81
HLA-A*30:01	KMFYKGVIT	KMFYKGVIM	551	216
HLA-C*02:02	ITHDVSSAI	IMHDVSSAI	375	833
HLA-C*02:10	ITHDVSSAI	IMHDVSSAI	375	833
HLA-A*02:11	FKMFYKGVIT	FKMFYKGVIM	322	152

NSP15 protein

T112I

Reference  DYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNA
           ||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated    DYKRDAPAHISTIGVCSMTDIAKKPIETICAPLTVFFDGRVDGQVDLFRNA

Export table to csv 

Allele	Reference peptide	Mutated peptide	Reference affinity (IC50, nM)	Mutated affinity (IC50, nM)
HLA-A*02:02	SMTDIAKKPT	SMTDIAKKPI	3353	375
HLA-A*68:02	TETICAPLTV	IETICAPLTV	64	161
HLA-C*03:03	IAKKPTETI	IAKKPIETI	497	1187
HLA-C*03:04	IAKKPTETI	IAKKPIETI	497	1187
HLA-A*02:11	SMTDIAKKPTETI	SMTDIAKKPIETI	560	263