Summary
This strain contains 25 protein-level mutations:
- 7 mutations in Spike protein: EFR 156-158 deletion, E484K, S494P, D614G, E780A, D839V, T1027I
- 3 mutations in N protein: A12S, 203-204 RG->KR, G212C
- 1 mutation in M protein: I82T
- 2 mutations in NS3 protein: S171L, V201F
- 1 mutation in NS9b protein: M8I
- 1 mutation in NS9c protein: G50N
- 1 mutation in NSP2 protein: N269D
- 3 mutations in NSP3 protein: M84V, R1297I, S1375Y
- 1 mutation in NSP6 protein: V84F
- 1 mutation in NSP10 protein: T111I
- 2 mutations in NSP12 protein: S229N, P323L
- 2 mutations in NSP13 protein: P77L, L455M
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
EFR 156-158 deletion
Reference CEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQ
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Mutated CEFQFCNDPFLGVYYHKNNKSWMES‑‑‑VYSSANNCTFEYVSQPFLMDLEGKQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | WMESEFRVY | WMESVY | 10 | - |
| HLA-B*45:01 | SEFRVYSSA | SVYSSA | 10 | - |
| HLA-B*18:01 | MESEFRVY | MESVY | 11 | - |
| HLA-B*50:01 | SEFRVYSSA | SVYSSA | 14 | - |
| HLA-A*31:01 | KSWMESEFR | KSWMES | 17 | - |
| HLA-A*29:02 | SEFRVYSSANNCTFEY | SVYSSANNCTFEY | - | 19 |
| HLA-B*40:06 | SEFRVYSSA | SVYSSA | 21 | - |
| HLA-A*30:02 | RVYSSANNCTFEY | - | 27 | - |
| HLA-B*40:02 | SEFRVYSSA | SVYSSA | 29 | - |
| HLA-A*29:02 | RVYSSANNCTFEY | - | 38 | - |
| HLA-B*15:03 | RVYSSANNCTF | - | 41 | - |
| HLA-A*29:02 | SWMESEFRVY | SWMESVY | 49 | - |
| HLA-B*45:01 | MESEFRVYS | MESVYS | 54 | - |
| HLA-B*15:01 | WMESEFRVY | WMESVY | 67 | - |
| HLA-A*68:01 | NKSWMESEFR | NKSWMES | 75 | - |
| HLA-B*15:01 | RVYSSANNCTF | - | 75 | - |
| HLA-A*32:01 | RVYSSANNCTF | - | 80 | - |
| HLA-C*03:02 | WMESEFRVY | WMESVY | 80 | - |
| HLA-A*30:02 | SEFRVYSSANNCTFEY | SVYSSANNCTFEY | - | 82 |
| HLA-A*29:02 | WMESEFRVY | WMESVY | 109 | - |
| HLA-C*14:02 | SWMESEFRVY | SWMESVY | 111 | - |
| HLA-A*31:01 | KNNKSWMESEFR | - | 126 | - |
| HLA-B*15:03 | NKSWMESEF | NKSWMES | 126 | - |
| HLA-B*58:01 | RVYSSANNCTF | - | 126 | - |
| HLA-B*15:03 | FRVYSSANNCTF | - | 131 | - |
| HLA-A*11:01 | SEFRVYSSANNCTFEY | SVYSSANNCTFEY | - | 138 |
| HLA-B*45:01 | ESEFRVYSSA | ESVYSSA | 138 | - |
| HLA-B*45:01 | MESEFRVYSS | MESVYSS | 143 | - |
| HLA-B*50:01 | MESEFRVYS | MESVYS | 146 | - |
| HLA-A*03:01 | RVYSSANNCTFEY | - | 151 | - |
| HLA-A*11:01 | RVYSSANNCTFEY | - | 151 | - |
| HLA-A*02:11 | WMESEFRV | WMESV | 156 | - |
| HLA-B*15:03 | MESEFRVY | MESVY | 180 | - |
| HLA-B*15:01 | RVYSSANNCTFEY | - | 182 | - |
| HLA-A*30:02 | SWMESEFRVY | SWMESVY | 183 | - |
| HLA-A*68:02 | ESEFRVYSSA | ESVYSSA | 187 | - |
| HLA-B*58:01 | KSWMESEFRV | KSWMESV | 187 | - |
| HLA-B*15:03 | SWMESEFRVY | SWMESVY | 196 | - |
| HLA-B*15:03 | RVYSSANNCTFEY | - | 203 | - |
| HLA-A*02:11 | KSWMESEFRV | KSWMESV | 212 | - |
| HLA-B*15:03 | MESEFRVYSSANNCTF | MESVYSSANNCTF | - | 219 |
| HLA-A*02:06 | SEFRVYSSANNCTFEYV | SVYSSANNCTFEYV | - | 223 |
| HLA-C*14:02 | YYHKNNKSWMESEFRVY | YYHKNNKSWMESVY | - | 223 |
| HLA-C*14:02 | YYHKNNKSWMESEF | - | 227 | - |
| HLA-B*15:03 | YHKNNKSWMESEFRVY | YHKNNKSWMESVY | - | 237 |
| HLA-A*02:11 | SEFRVYSSANNCTFEYV | SVYSSANNCTFEYV | - | 249 |
| HLA-A*30:02 | FRVYSSANNCTFEY | - | 249 | - |
| HLA-A*30:01 | RVYSSANNC | - | 250 | - |
| HLA-B*15:03 | HKNNKSWMESEFRVY | HKNNKSWMESVY | - | 252 |
| HLA-A*02:06 | KSWMESEFRV | KSWMESV | 255 | - |
| HLA-A*02:11 | SWMESEFRV | SWMESV | 262 | - |
| HLA-A*02:11 | RVYSSANNCTFEYV | - | 267 | - |
| HLA-B*58:01 | MESEFRVYSSANNCTF | MESVYSSANNCTF | - | 267 |
| HLA-A*02:06 | RVYSSANNCTFEYV | - | 274 | - |
| HLA-A*11:01 | KSWMESEFR | KSWMES | 279 | - |
| HLA-A*03:02 | RVYSSANNCTFEY | - | 282 | - |
| HLA-A*02:02 | WMESEFRVYS | WMESVYS | 286 | - |
| HLA-A*68:02 | SEFRVYSSANNCTFEYV | SVYSSANNCTFEYV | - | 289 |
| HLA-A*33:03 | NKSWMESEFR | NKSWMES | 293 | - |
| HLA-B*40:06 | MESEFRVYS | MESVYS | 299 | - |
| HLA-B*18:01 | SEFRVYSSA | SVYSSA | 303 | - |
| HLA-B*15:01 | SWMESEFRVY | SWMESVY | 308 | - |
| HLA-B*50:01 | ESEFRVYSSA | ESVYSSA | 314 | - |
| HLA-A*02:02 | WMESEFRV | WMESV | 320 | - |
| HLA-B*35:01 | WMESEFRVY | WMESVY | 336 | - |
| HLA-B*18:01 | MESEFRVYS | MESVYS | 338 | - |
| HLA-A*33:03 | NNKSWMESEFR | - | 340 | - |
| HLA-A*30:02 | WMESEFRVY | WMESVY | 342 | - |
| HLA-B*40:02 | MESEFRVYS | MESVYS | 383 | - |
| HLA-B*45:01 | SEFRVYSSAN | SVYSSAN | 384 | - |
| HLA-C*14:02 | WMESEFRVY | WMESVY | 387 | - |
| HLA-A*31:01 | NKSWMESEFR | NKSWMES | 402 | - |
| HLA-B*40:02 | SEFRVYSS | SVYSS | 414 | - |
| HLA-B*50:01 | SEFRVYSSAN | SVYSSAN | 423 | - |
| HLA-B*44:03 | MESEFRVY | MESVY | 439 | - |
| HLA-B*15:01 | SEFRVYSSANNCTFEY | SVYSSANNCTFEY | - | 457 |
| HLA-A*23:01 | SWMESEFRV | SWMESV | 458 | - |
| HLA-B*57:01 | KSWMESEFRV | KSWMESV | 461 | - |
| HLA-A*03:02 | KSWMESEFR | KSWMES | 465 | - |
| HLA-B*58:01 | KSWMESEF | KSWMES | 465 | - |
| HLA-B*57:01 | RVYSSANNCTF | - | 493 | - |
| HLA-B*15:03 | NKSWMESEFRVY | NKSWMESVY | 1077 | 29 |
| HLA-B*15:01 | SEFRVYSSANNCTF | SVYSSANNCTF | 3491 | 137 |
| HLA-B*15:03 | NNKSWMESEFRVY | NNKSWMESVY | 6334 | 342 |
| HLA-B*15:03 | KNNKSWMESEFRVY | KNNKSWMESVY | 2936 | 190 |
| HLA-A*02:02 | WMESEFRVYSSA | WMESVYSSA | 1287 | 97 |
| HLA-A*02:11 | WMESEFRVYSSA | WMESVYSSA | 1678 | 151 |
| HLA-B*57:01 | KSWMESEFRVY | KSWMESVY | 181 | 1527 |
| HLA-B*58:01 | KSWMESEFRVY | KSWMESVY | 167 | 832 |
| HLA-B*15:03 | SEFRVYSSANNCTF | SVYSSANNCTF | 563 | 125 |
| HLA-A*30:02 | KNNKSWMESEFRVY | KNNKSWMESVY | 963 | 285 |
| HLA-A*30:01 | KSWMESEFRVYS | KSWMESVYS | 1192 | 439 |
| HLA-B*45:01 | MESEFRVYSSA | MESVYSSA | 73 | 177 |
| HLA-B*40:06 | MESEFRVYSSA | MESVYSSA | 248 | 549 |
| HLA-B*40:02 | MESEFRVYSSA | MESVYSSA | 453 | 951 |
E484K
Reference SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
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Mutated SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQPYGFQPTNGVGYQPYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:02 | VEGFNCYFPL | VKGFNCYFPL | 31 | 7011 |
| HLA-B*40:01 | VEGFNCYFPL | VKGFNCYFPL | 76 | 15210 |
| HLA-A*11:01 | GSTPCNGVE | GSTPCNGVK | 32767 | 444 |
| HLA-A*68:02 | EGFNCYFPL | KGFNCYFPL | 117 | 3877 |
| HLA-A*30:01 | EGFNCYFPL | KGFNCYFPL | 5413 | 164 |
| HLA-A*32:01 | EGFNCYFPL | KGFNCYFPL | 6548 | 210 |
| HLA-B*15:01 | GVEGFNCYF | GVKGFNCYF | 3125 | 132 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQPY | 1806 | 79 |
| HLA-A*31:01 | EGFNCYFPL | KGFNCYFPL | 4227 | 226 |
| HLA-B*15:03 | EGFNCYFPL | KGFNCYFPL | 2980 | 212 |
| HLA-B*15:03 | EGFNCYFPLQSY | KGFNCYFPLQPY | 5675 | 432 |
| HLA-A*02:11 | EGFNCYFPL | KGFNCYFPL | 2508 | 259 |
| HLA-B*15:03 | GVEGFNCYF | GVKGFNCYF | 1657 | 199 |
| HLA-A*02:06 | EGFNCYFPL | KGFNCYFPL | 1266 | 169 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQPY | 1081 | 159 |
| HLA-B*35:01 | TPCNGVEGF | TPCNGVKGF | 319 | 1754 |
| HLA-B*35:01 | NGVEGFNCY | NGVKGFNCY | 346 | 1439 |
| HLA-C*16:01 | EGFNCYFPL | KGFNCYFPL | 1861 | 498 |
| HLA-B*15:03 | VEGFNCYFPL | VKGFNCYFPL | 943 | 280 |
S494P
Reference STEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLH
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Mutated STEIYQAGSTPCNGVKGFNCYFPLQPYGFQPTNGVGYQPYRVVVLSFELLH
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | QSYGFQPTNGV | QPYGFQPTNGV | 122 | 3957 |
| HLA-B*35:01 | QSYGFQPTNGVGY | QPYGFQPTNGVGY | 11541 | 482 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQPY | 1806 | 79 |
| HLA-A*30:02 | QSYGFQPTNGVGY | QPYGFQPTNGVGY | 258 | 4144 |
| HLA-B*15:03 | EGFNCYFPLQSY | KGFNCYFPLQPY | 5675 | 432 |
| HLA-B*15:03 | LQSYGFQPT | LQPYGFQPT | 138 | 1790 |
| HLA-B*15:03 | LQSYGFQPTNGVGY | LQPYGFQPTNGVGY | 113 | 1113 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQPY | 1081 | 159 |
| HLA-B*15:01 | LQSYGFQPTNGVGY | LQPYGFQPTNGVGY | 180 | 970 |
| HLA-A*02:06 | LQSYGFQPT | LQPYGFQPT | 339 | 940 |
| HLA-B*35:01 | FPLQSYGF | FPLQPYGF | 197 | 475 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
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Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
E780A
Reference QYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQI
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Mutated QYGSFCTQLNRALTGIAVEQDKNTQAVFAQVKQIYKTPPIKDFGGFNFSQI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*44:03 | QEVFAQVKQIY | QAVFAQVKQIY | 212 | 29037 |
| HLA-B*44:02 | QEVFAQVKQI | QAVFAQVKQI | 233 | 24853 |
| HLA-B*44:02 | QEVFAQVKQIY | QAVFAQVKQIY | 279 | 26652 |
| HLA-B*44:03 | QEVFAQVKQI | QAVFAQVKQI | 336 | 30020 |
| HLA-B*40:02 | QEVFAQVKQI | QAVFAQVKQI | 423 | 32055 |
| HLA-B*45:01 | AVEQDKNTQE | AVEQDKNTQA | 22903 | 306 |
| HLA-B*45:01 | VEQDKNTQE | VEQDKNTQA | 21254 | 375 |
| HLA-A*03:01 | EVFAQVKQIYK | AVFAQVKQIYK | 1493 | 28 |
| HLA-A*26:01 | EVFAQVKQIY | AVFAQVKQIY | 116 | 4802 |
| HLA-A*03:02 | EVFAQVKQIYK | AVFAQVKQIYK | 1238 | 41 |
| HLA-A*11:01 | EVFAQVKQIYKT | AVFAQVKQIYKT | 8182 | 278 |
| HLA-A*68:02 | EVFAQVKQI | AVFAQVKQI | 75 | 2170 |
| HLA-A*03:01 | QEVFAQVKQIYK | QAVFAQVKQIYK | 6882 | 244 |
| HLA-A*02:11 | EVFAQVKQI | AVFAQVKQI | 6830 | 291 |
| HLA-A*11:01 | EVFAQVKQIYK | AVFAQVKQIYK | 207 | 9 |
| HLA-A*68:01 | EVFAQVKQIYK | AVFAQVKQIYK | 15 | 341 |
| HLA-A*11:01 | QEVFAQVKQIYK | QAVFAQVKQIYK | 2380 | 108 |
| HLA-A*68:01 | EVFAQVKQIYKT | AVFAQVKQIYKT | 355 | 6478 |
| HLA-A*03:02 | QEVFAQVKQIYK | QAVFAQVKQIYK | 5488 | 335 |
| HLA-B*15:01 | EVFAQVKQIY | AVFAQVKQIY | 2157 | 160 |
| HLA-A*30:01 | EVFAQVKQIYK | AVFAQVKQIYK | 5393 | 467 |
| HLA-B*15:03 | EVFAQVKQIY | AVFAQVKQIY | 4142 | 429 |
| HLA-A*11:01 | TQEVFAQVK | TQAVFAQVK | 1140 | 136 |
| HLA-A*30:02 | EVFAQVKQIY | AVFAQVKQIY | 1571 | 219 |
| HLA-A*11:01 | TQEVFAQVKQIYK | TQAVFAQVKQIYK | 1379 | 226 |
| HLA-B*15:03 | TQEVFAQVKQIY | TQAVFAQVKQIY | 533 | 100 |
| HLA-A*30:01 | KNTQEVFAQV | KNTQAVFAQV | 970 | 248 |
| HLA-A*29:02 | EVFAQVKQIY | AVFAQVKQIY | 893 | 268 |
| HLA-B*15:01 | EQDKNTQEVF | EQDKNTQAVF | 923 | 284 |
| HLA-B*15:01 | TQEVFAQVKQIY | TQAVFAQVKQIY | 695 | 226 |
| HLA-A*02:11 | KNTQEVFAQV | KNTQAVFAQV | 162 | 447 |
| HLA-B*15:03 | EQDKNTQEVF | EQDKNTQAVF | 1161 | 428 |
| HLA-A*03:02 | NTQEVFAQVK | NTQAVFAQVK | 856 | 333 |
| HLA-A*02:11 | NTQEVFAQV | NTQAVFAQV | 25 | 60 |
| HLA-A*11:01 | NTQEVFAQVK | NTQAVFAQVK | 148 | 64 |
| HLA-A*11:01 | NTQEVFAQVKQIYK | NTQAVFAQVKQIYK | 729 | 322 |
| HLA-A*02:06 | KNTQEVFAQV | KNTQAVFAQV | 174 | 365 |
D839V
Reference KRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPL
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Mutated KRSFIEDLLFNKVTLADAGFIKQYGVCLGDIAARDLICAQKFNGLTVLPPL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | TLADAGFIKQYGD | TLADAGFIKQYGV | 26741 | 36 |
| HLA-A*02:02 | TLADAGFIKQYGD | TLADAGFIKQYGV | 15114 | 27 |
| HLA-A*68:02 | DAGFIKQYGD | DAGFIKQYGV | 32062 | 145 |
| HLA-A*02:01 | TLADAGFIKQYGD | TLADAGFIKQYGV | 27682 | 179 |
| HLA-A*31:01 | KQYGDCLGDIAAR | KQYGVCLGDIAAR | 586 | 94 |
| HLA-B*08:01 | FIKQYGDCL | FIKQYGVCL | 238 | 55 |
| HLA-B*42:01 | FIKQYGDCL | FIKQYGVCL | 1448 | 454 |
| HLA-A*02:11 | KQYGDCLGDI | KQYGVCLGDI | 594 | 248 |
T1027I
Reference QSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSF
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Mutated QSLQTYVTQQLIRAAEIRASANLAAIKMSECVLGQSKRVDFCGKGYHLMSF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*03:03 | RASANLAAT | RASANLAAI | 4611 | 62 |
| HLA-C*03:04 | RASANLAAT | RASANLAAI | 4611 | 62 |
| HLA-A*32:01 | RASANLAAT | RASANLAAI | 22711 | 434 |
| HLA-C*12:03 | RASANLAAT | RASANLAAI | 2694 | 60 |
| HLA-C*17:01 | RASANLAAT | RASANLAAI | 11235 | 278 |
| HLA-C*15:02 | RASANLAAT | RASANLAAI | 6895 | 180 |
| HLA-C*16:01 | RASANLAAT | RASANLAAI | 924 | 25 |
| HLA-B*40:01 | AEIRASANLAAT | AEIRASANLAAI | 8508 | 344 |
| HLA-C*03:02 | RASANLAAT | RASANLAAI | 2899 | 129 |
| HLA-B*58:01 | RASANLAAT | RASANLAAI | 5883 | 333 |
| HLA-B*15:03 | RASANLAAT | RASANLAAI | 3151 | 184 |
| HLA-B*44:02 | AEIRASANLAAT | AEIRASANLAAI | 6113 | 368 |
| HLA-C*12:02 | RASANLAAT | RASANLAAI | 5044 | 305 |
| HLA-B*40:02 | AEIRASANLAAT | AEIRASANLAAI | 1973 | 232 |
| HLA-A*02:06 | RASANLAAT | RASANLAAI | 2857 | 336 |
| HLA-A*30:01 | ATKMSECVL | AIKMSECVL | 173 | 1150 |
| HLA-C*16:01 | ATKMSECVL | AIKMSECVL | 476 | 2866 |
| HLA-A*30:01 | ATKMSECVLGQSK | AIKMSECVLGQSK | 464 | 2739 |
| HLA-A*30:01 | RASANLAAT | RASANLAAI | 1238 | 321 |
| HLA-A*02:02 | NLAATKMSECVL | NLAAIKMSECVL | 877 | 285 |
| HLA-A*02:02 | NLAATKMSECV | NLAAIKMSECV | 702 | 236 |
| HLA-A*02:11 | NLAATKMSECV | NLAAIKMSECV | 647 | 232 |
N protein
203-204 RG->KR
Reference GGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ
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Mutated GGSQASSRSSSRSRNSSRNSTPGSSKRTSPARMACNGGDAALALLLLDRLNQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | NSTPGSSRG | NSTPGSSKR | 32767 | 34 |
| HLA-A*33:03 | NSTPGSSRG | NSTPGSSKR | 32767 | 300 |
| HLA-B*15:03 | SRGTSPARM | SKRTSPARM | 2669 | 106 |
| HLA-A*31:01 | RNSTPGSSRG | RNSTPGSSKR | 7013 | 340 |
| HLA-A*30:01 | SSRNSTPGSSR | SSRNSTPGSSK | 356 | 20 |
| HLA-A*31:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 3757 | 231 |
| HLA-A*31:01 | SSRNSTPGSSR | SSRNSTPGSSK | 127 | 1853 |
| HLA-A*30:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 4255 | 353 |
| HLA-A*30:01 | RNSTPGSSR | RNSTPGSSK | 2120 | 188 |
| HLA-A*31:01 | RNSTPGSSR | RNSTPGSSK | 123 | 1335 |
| HLA-A*03:01 | SSRNSTPGSSR | SSRNSTPGSSK | 4753 | 442 |
| HLA-A*11:01 | SSRNSTPGSSR | SSRNSTPGSSK | 2231 | 263 |
| HLA-A*68:01 | NSSRNSTPGSSR | NSSRNSTPGSSK | 270 | 1385 |
| HLA-A*30:01 | RGTSPARMAG | KRTSPARMAC | 226 | 1004 |
| HLA-A*30:01 | GTSPARMAG | RTSPARMAC | 493 | 116 |
| HLA-A*30:01 | SSRGTSPARMAG | SSKRTSPARMAC | 449 | 1719 |
| HLA-A*30:01 | GSSRGTSPA | GSSKRTSPA | 555 | 145 |
| HLA-A*30:01 | SSRNSTPGSSRG | SSRNSTPGSSKR | 1625 | 469 |
G212C
Reference SSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGK
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated SSRSRNSSRNSTPGSSKRTSPARMACNGGDAALALLLLDRLNQLESKMSGK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | RGTSPARMAG | KRTSPARMAC | 226 | 1004 |
| HLA-A*30:01 | GTSPARMAG | RTSPARMAC | 493 | 116 |
| HLA-A*30:01 | SSRGTSPARMAG | SSKRTSPARMAC | 449 | 1719 |
| HLA-B*27:05 | ARMAGNGGDAAL | ARMACNGGDAAL | 406 | 201 |
M protein
I82T
Reference LWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LWPVTLACFVLAAVYRINWITGGIATAMACLVGLMWLSYFIASFRLFARTR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | NWITGGIAI | NWITGGIAT | 109 | 4410 |
| HLA-A*68:02 | IAMACLVGL | TAMACLVGL | 424 | 40 |
| HLA-B*27:05 | YRINWITGGIAI | YRINWITGGIAT | 85 | 885 |
| HLA-A*68:02 | AIAMACLVGL | ATAMACLVGL | 1414 | 203 |
| HLA-A*02:11 | WITGGIAIA | WITGGIATA | 788 | 138 |
| HLA-A*68:02 | IAMACLVGLM | TAMACLVGLM | 2691 | 495 |
| HLA-A*02:06 | WITGGIAIA | WITGGIATA | 477 | 117 |
| HLA-A*02:01 | AIAMACLVGL | ATAMACLVGL | 97 | 388 |
| HLA-A*02:06 | IAIAMACLV | IATAMACLV | 90 | 359 |
| HLA-A*02:11 | AIAMACLVGL | ATAMACLVGL | 53 | 208 |
| HLA-A*02:02 | WITGGIAIA | WITGGIATA | 333 | 88 |
| HLA-A*02:11 | IAIAMACLV | IATAMACLV | 140 | 524 |
| HLA-A*02:02 | AIAMACLVGL | ATAMACLVGL | 41 | 133 |
| HLA-B*58:01 | IAMACLVGL | TAMACLVGL | 423 | 1182 |
| HLA-A*02:11 | GIAIAMACL | GIATAMACL | 350 | 144 |
| HLA-A*68:02 | WITGGIAIA | WITGGIATA | 918 | 378 |
| HLA-B*58:01 | IAMACLVGLM | TAMACLVGLM | 479 | 1090 |
| HLA-C*12:03 | IAIAMACLV | IATAMACLV | 47 | 105 |
| HLA-A*02:02 | GIAIAMACL | GIATAMACL | 110 | 50 |
| HLA-B*57:01 | IAMACLVGLMW | TAMACLVGLMW | 65 | 141 |
| HLA-A*02:02 | GIAIAMACLV | GIATAMACLV | 84 | 39 |
| HLA-C*12:02 | IAIAMACLV | IATAMACLV | 336 | 715 |
| HLA-A*02:06 | GIAIAMACL | GIATAMACL | 662 | 317 |
| HLA-A*02:02 | GGIAIAMACL | GGIATAMACL | 596 | 288 |
| HLA-B*58:01 | IAMACLVGLMW | TAMACLVGLMW | 19 | 39 |
| HLA-A*68:02 | IAIAMACLV | IATAMACLV | 168 | 339 |
| HLA-A*02:01 | IAMACLVGL | TAMACLVGL | 122 | 246 |
| HLA-A*02:11 | IAMACLVGL | TAMACLVGL | 70 | 141 |
| HLA-B*57:01 | AIAMACLVGLMW | ATAMACLVGLMW | 538 | 268 |
NS3 protein
S171L
Reference FLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FLCWHTNCYDYCIPYNSVTSSIVITLGDGTTSPISEHDYQIGGYTEKWESG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*17:01 | VTSSIVITS | VTSSIVITL | 13914 | 69 |
| HLA-C*03:03 | VTSSIVITS | VTSSIVITL | 19726 | 117 |
| HLA-C*03:04 | VTSSIVITS | VTSSIVITL | 19726 | 117 |
| HLA-A*32:01 | VTSSIVITS | VTSSIVITL | 13457 | 98 |
| HLA-C*16:01 | VTSSIVITS | VTSSIVITL | 4293 | 36 |
| HLA-A*02:11 | TSGDGTTSPI | TLGDGTTSPI | 10425 | 92 |
| HLA-A*02:11 | VTSSIVITS | VTSSIVITL | 8785 | 89 |
| HLA-A*02:02 | TSGDGTTSPI | TLGDGTTSPI | 8938 | 95 |
| HLA-C*12:03 | VTSSIVITS | VTSSIVITL | 4793 | 51 |
| HLA-C*15:02 | VTSSIVITS | VTSSIVITL | 7397 | 89 |
| HLA-C*03:02 | VTSSIVITS | VTSSIVITL | 9955 | 175 |
| HLA-A*02:02 | VTSSIVITS | VTSSIVITL | 5314 | 103 |
| HLA-C*16:01 | TSSIVITS | TSSIVITL | 18534 | 362 |
| HLA-B*42:01 | IPYNSVTSSIVITS | IPYNSVTSSIVITL | 14524 | 360 |
| HLA-A*02:11 | SVTSSIVITS | SVTSSIVITL | 12237 | 410 |
| HLA-A*02:02 | YNSVTSSIVITS | YNSVTSSIVITL | 8810 | 301 |
| HLA-A*02:02 | SVTSSIVITS | SVTSSIVITL | 5294 | 197 |
| HLA-C*12:02 | VTSSIVITS | VTSSIVITL | 9810 | 369 |
| HLA-B*58:01 | VTSSIVITS | VTSSIVITL | 2482 | 96 |
| HLA-A*02:06 | VTSSIVITS | VTSSIVITL | 4179 | 183 |
| HLA-A*68:02 | SVTSSIVITS | SVTSSIVITL | 1418 | 92 |
| HLA-A*68:02 | YNSVTSSIVITS | YNSVTSSIVITL | 2711 | 182 |
| HLA-A*68:02 | VTSSIVITS | VTSSIVITL | 911 | 63 |
| HLA-A*68:02 | NSVTSSIVITS | NSVTSSIVITL | 5500 | 388 |
| HLA-C*08:02 | SGDGTTSPI | LGDGTTSPI | 222 | 110 |
V201F
Reference TSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVE
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TSPISEHDYQIGGYTEKWESGVKDCFVLHSYFTSDYYQLYSTQLSTDTGVE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*18:01 | WESGVKDCV | WESGVKDCF | 2585 | 99 |
| HLA-A*02:06 | VVLHSYFTS | FVLHSYFTS | 1279 | 71 |
| HLA-B*44:03 | WESGVKDCV | WESGVKDCF | 5622 | 381 |
| HLA-A*02:11 | VVLHSYFTS | FVLHSYFTS | 3221 | 231 |
| HLA-B*44:02 | WESGVKDCV | WESGVKDCF | 4987 | 439 |
| HLA-C*03:02 | VVLHSYFTSDYY | FVLHSYFTSDYY | 3246 | 308 |
| HLA-A*68:01 | VVLHSYFTSDYY | FVLHSYFTSDYY | 2255 | 420 |
| HLA-A*01:01 | VVLHSYFTSDYY | FVLHSYFTSDYY | 599 | 122 |
| HLA-A*29:02 | VVLHSYFTSDY | FVLHSYFTSDY | 262 | 67 |
| HLA-B*40:01 | WESGVKDCVV | WESGVKDCFV | 327 | 1182 |
| HLA-A*29:02 | VVLHSYFTSDYY | FVLHSYFTSDYY | 73 | 22 |
| HLA-A*29:02 | CVVLHSYFTSDY | CFVLHSYFTSDY | 1054 | 385 |
| HLA-A*29:02 | CVVLHSYFTSDYY | CFVLHSYFTSDYY | 328 | 129 |
| HLA-B*15:01 | VVLHSYFTSDYY | FVLHSYFTSDYY | 1227 | 495 |
| HLA-A*30:02 | KDCVVLHSY | KDCFVLHSY | 888 | 374 |
| HLA-A*30:02 | VVLHSYFTSDY | FVLHSYFTSDY | 335 | 764 |
| HLA-B*15:03 | VKDCVVLHSYF | VKDCFVLHSYF | 817 | 373 |
| HLA-B*40:02 | WESGVKDCVV | WESGVKDCFV | 250 | 535 |
NS9b protein
M8I
Reference MDPKISEMHPALRLVDPQIQLAVTRMENAVGRD
||||||| |||||||||||||||||||||||||
Mutated MDPKISEIHPALRLVDPQIQLAVTRMENAVGRD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | SEMHPALRLV | SEIHPALRLV | 122 | 1116 |
| HLA-A*02:02 | SEMHPALRLV | SEIHPALRLV | 198 | 1570 |
| HLA-A*68:02 | EMHPALRLV | EIHPALRLV | 211 | 31 |
| HLA-A*02:11 | EMHPALRLV | EIHPALRLV | 249 | 1492 |
| HLA-B*44:02 | ISEMHPALRL | ISEIHPALRL | 493 | 2556 |
| HLA-B*44:02 | SEMHPALRLV | SEIHPALRLV | 47 | 207 |
| HLA-B*44:02 | SEMHPALRL | SEIHPALRL | 32 | 127 |
| HLA-B*45:01 | SEMHPALRLV | SEIHPALRLV | 70 | 271 |
| HLA-B*45:01 | SEMHPALRL | SEIHPALRL | 338 | 1204 |
| HLA-B*44:03 | SEMHPALRLV | SEIHPALRLV | 70 | 248 |
| HLA-A*02:02 | MHPALRLVDPQIQL | IHPALRLVDPQIQL | 325 | 989 |
| HLA-B*44:03 | SEMHPALRL | SEIHPALRL | 35 | 104 |
| HLA-A*68:01 | KISEMHPALR | KISEIHPALR | 312 | 107 |
| HLA-A*02:02 | MDPKISEMHPAL | MDPKISEIHPAL | 1080 | 452 |
| HLA-B*15:03 | SEMHPALRL | SEIHPALRL | 493 | 1167 |
| HLA-B*40:02 | ISEMHPALRL | ISEIHPALRL | 396 | 874 |
| HLA-B*40:02 | SEMHPALRLV | SEIHPALRLV | 75 | 153 |
| HLA-A*02:01 | KISEMHPAL | KISEIHPAL | 139 | 69 |
| HLA-A*02:11 | MHPALRLVDPQIQL | IHPALRLVDPQIQL | 486 | 979 |
| HLA-A*02:11 | KISEMHPAL | KISEIHPAL | 14 | 7 |
NS9c protein
G50N
Reference AAVKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
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Mutated AAVKPLLVPHHVVATVQEIQLQAAVNELLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*18:01 | GELLLLEWLAM | NELLLLEWLAM | 4979 | 247 |
| HLA-B*18:01 | GELLLLEW | NELLLLEW | 3915 | 401 |
| HLA-B*40:01 | GELLLLEWL | NELLLLEWL | 28 | 140 |
| HLA-A*02:11 | QLQAAVGEL | QLQAAVNEL | 120 | 27 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVNEL | 338 | 81 |
| HLA-B*40:02 | GELLLLEWL | NELLLLEWL | 52 | 204 |
| HLA-B*40:02 | GELLLLEWLAM | NELLLLEWLAM | 201 | 772 |
| HLA-A*02:01 | QLQAAVGEL | QLQAAVNEL | 1290 | 358 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVNEL | 92 | 28 |
| HLA-B*58:01 | VGELLLLEW | VNELLLLEW | 330 | 1071 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVNEL | 170 | 58 |
| HLA-A*02:01 | LQAAVGELL | LQAAVNELL | 938 | 352 |
| HLA-A*02:02 | LQAAVGELL | LQAAVNELL | 44 | 17 |
| HLA-B*40:06 | GELLLLEWLA | NELLLLEWLA | 268 | 677 |
| HLA-B*40:02 | GELLLLEWLA | NELLLLEWLA | 475 | 1195 |
| HLA-A*02:11 | LQAAVGELL | LQAAVNELL | 386 | 154 |
| HLA-A*02:01 | IQLQAAVGEL | IQLQAAVNEL | 1138 | 475 |
| HLA-A*02:01 | QLQAAVGELL | QLQAAVNELL | 961 | 403 |
| HLA-A*02:01 | GELLLLEWLAMA | NELLLLEWLAMA | 109 | 258 |
| HLA-A*02:02 | QLQAAVGELL | QLQAAVNELL | 35 | 15 |
| HLA-A*02:02 | IQLQAAVGELL | IQLQAAVNELL | 724 | 313 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVNELL | 303 | 131 |
| HLA-B*58:01 | AVGELLLLEW | AVNELLLLEW | 196 | 87 |
| HLA-A*02:11 | GELLLLEWLAMA | NELLLLEWLAMA | 29 | 61 |
| HLA-B*57:01 | AVGELLLLEW | AVNELLLLEW | 432 | 206 |
| HLA-A*68:02 | EIQLQAAVGELL | EIQLQAAVNELL | 697 | 346 |
NSP2 protein
N269D
Reference VPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIA
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VPRASANIGCNHTGVVGEGSEGLNDDLLEILQKEKVNINIVGDFKLNEEIA
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | NLLEILQKEKV | DLLEILQKEKV | 36 | 2285 |
| HLA-A*02:11 | NLLEILQKEKVNI | DLLEILQKEKVNI | 117 | 5028 |
| HLA-A*02:01 | NLLEILQKEKV | DLLEILQKEKV | 200 | 7808 |
| HLA-A*02:06 | NLLEILQKEKV | DLLEILQKEKV | 399 | 8785 |
| HLA-A*02:02 | NLLEILQKEKV | DLLEILQKEKV | 429 | 7531 |
| HLA-B*40:01 | SEGLNDNLL | SEGLNDDLL | 129 | 397 |
NSP3 protein
M84V
Reference DAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPP
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated DAVIKTLQPVSELLTPLGIDLDEWSVATYYLFDESGEFKLASHMYCSFYPP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | LLTPLGIDLDEWSM | LLTPLGIDLDEWSV | 5009 | 138 |
| HLA-A*02:11 | GIDLDEWSM | GIDLDEWSV | 2256 | 71 |
| HLA-B*15:03 | SMATYYLF | SVATYYLF | 82 | 1589 |
| HLA-A*02:01 | GIDLDEWSM | GIDLDEWSV | 5812 | 318 |
| HLA-B*35:01 | MATYYLFDESGEF | VATYYLFDESGEF | 487 | 8842 |
| HLA-A*02:11 | LGIDLDEWSM | LGIDLDEWSV | 8171 | 489 |
| HLA-A*02:01 | LLTPLGIDLDEWSM | LLTPLGIDLDEWSV | 7346 | 500 |
| HLA-A*02:02 | LLTPLGIDLDEWSM | LLTPLGIDLDEWSV | 2882 | 230 |
| HLA-A*68:01 | MATYYLFDESGEFK | VATYYLFDESGEFK | 257 | 2976 |
| HLA-A*02:06 | GIDLDEWSM | GIDLDEWSV | 4057 | 362 |
| HLA-A*02:06 | LGIDLDEWSM | LGIDLDEWSV | 4769 | 481 |
| HLA-A*24:02 | SMATYYLF | SVATYYLF | 167 | 1513 |
| HLA-B*15:03 | SMATYYLFDESGEF | SVATYYLFDESGEF | 275 | 2226 |
| HLA-A*32:01 | WSMATYYLF | WSVATYYLF | 40 | 313 |
| HLA-B*15:01 | SMATYYLF | SVATYYLF | 485 | 3569 |
| HLA-A*23:01 | SMATYYLF | SVATYYLF | 82 | 594 |
| HLA-A*24:02 | WSMATYYLF | WSVATYYLF | 17 | 114 |
| HLA-A*23:01 | LDEWSMATYYLF | LDEWSVATYYLF | 233 | 1244 |
| HLA-C*03:02 | MATYYLFDESGEF | VATYYLFDESGEF | 416 | 1869 |
| HLA-B*15:03 | MATYYLFDESGEF | VATYYLFDESGEF | 309 | 1388 |
| HLA-A*29:02 | WSMATYYLF | WSVATYYLF | 17 | 73 |
| HLA-A*23:01 | WSMATYYLF | WSVATYYLF | 8 | 34 |
| HLA-C*16:01 | WSMATYYL | WSVATYYL | 160 | 663 |
| HLA-A*30:02 | WSMATYYLF | WSVATYYLF | 318 | 1219 |
| HLA-B*15:01 | SMATYYLFDESGEF | SVATYYLFDESGEF | 350 | 1328 |
| HLA-B*53:01 | WSMATYYLF | WSVATYYLF | 123 | 441 |
| HLA-B*15:01 | MATYYLFDESGEF | VATYYLFDESGEF | 274 | 980 |
| HLA-A*23:01 | DEWSMATYYLF | DEWSVATYYLF | 263 | 904 |
| HLA-C*16:01 | WSMATYYLF | WSVATYYLF | 68 | 233 |
| HLA-B*57:01 | LDEWSMATYYLF | LDEWSVATYYLF | 109 | 369 |
| HLA-A*02:06 | WSMATYYLF | WSVATYYLF | 316 | 1065 |
| HLA-B*57:01 | EWSMATYYLF | EWSVATYYLF | 343 | 1070 |
| HLA-B*35:01 | WSMATYYLF | WSVATYYLF | 51 | 154 |
| HLA-B*58:01 | EWSMATYYLF | EWSVATYYLF | 157 | 467 |
| HLA-B*57:01 | WSMATYYLF | WSVATYYLF | 28 | 83 |
| HLA-B*58:01 | IDLDEWSMATYYLF | IDLDEWSVATYYLF | 380 | 1116 |
| HLA-B*58:01 | SMATYYLF | SVATYYLF | 493 | 1427 |
| HLA-B*58:01 | WSMATYYLFD | WSVATYYLFD | 490 | 1374 |
| HLA-B*58:01 | LDEWSMATYYLF | LDEWSVATYYLF | 65 | 180 |
| HLA-B*15:01 | WSMATYYLF | WSVATYYLF | 59 | 156 |
| HLA-B*44:02 | DEWSMATYYLF | DEWSVATYYLF | 265 | 679 |
| HLA-A*29:02 | EWSMATYYLF | EWSVATYYLF | 125 | 294 |
| HLA-A*01:01 | DLDEWSMATY | DLDEWSVATY | 107 | 247 |
| HLA-B*15:03 | WSMATYYLF | WSVATYYLF | 9 | 20 |
| HLA-B*58:01 | WSMATYYLF | WSVATYYLF | 7 | 15 |
| HLA-A*24:02 | EWSMATYYLF | EWSVATYYLF | 10 | 21 |
| HLA-C*03:03 | WSMATYYLF | WSVATYYLF | 301 | 619 |
| HLA-C*03:04 | WSMATYYLF | WSVATYYLF | 301 | 619 |
| HLA-A*23:01 | EWSMATYYLF | EWSVATYYLF | 7 | 14 |
R1297I
Reference VGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIAN
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VGHTDLMAAYVDNSSLTIKKPNELSIVLGLKTLATHGLAAVNSVPWDTIAN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | TIKKPNELSR | TIKKPNELSI | 125 | 32511 |
| HLA-A*31:01 | TIKKPNELSR | TIKKPNELSI | 156 | 28666 |
| HLA-A*33:03 | TIKKPNELSR | TIKKPNELSI | 249 | 32767 |
| HLA-B*27:05 | SRVLGLKTL | SIVLGLKTL | 302 | 30267 |
| HLA-A*68:01 | LTIKKPNELSR | LTIKKPNELSI | 411 | 32767 |
| HLA-A*31:01 | SSLTIKKPNELSR | SSLTIKKPNELSI | 358 | 27971 |
| HLA-B*15:03 | IKKPNELSR | IKKPNELSI | 19519 | 293 |
| HLA-B*27:05 | SRVLGLKTLA | SIVLGLKTLA | 456 | 30089 |
| HLA-C*06:02 | SRVLGLKTL | SIVLGLKTL | 271 | 16981 |
| HLA-C*07:01 | SRVLGLKTL | SIVLGLKTL | 355 | 17015 |
| HLA-C*07:02 | SRVLGLKTL | SIVLGLKTL | 481 | 13654 |
| HLA-A*30:01 | RVLGLKTLA | IVLGLKTLA | 136 | 3432 |
| HLA-C*03:03 | SRVLGLKTL | SIVLGLKTL | 10030 | 444 |
| HLA-C*03:04 | SRVLGLKTL | SIVLGLKTL | 10030 | 444 |
| HLA-A*30:01 | LSRVLGLK | LSIVLGLK | 305 | 4257 |
| HLA-A*02:11 | SLTIKKPNELSRVL | SLTIKKPNELSIVL | 5804 | 464 |
| HLA-A*02:02 | SLTIKKPNELSRVL | SLTIKKPNELSIVL | 2022 | 212 |
| HLA-B*35:03 | KPNELSRVL | KPNELSIVL | 1504 | 199 |
| HLA-B*44:03 | NELSRVLGL | NELSIVLGL | 435 | 72 |
| HLA-B*35:01 | KPNELSRVL | KPNELSIVL | 821 | 139 |
| HLA-B*58:01 | LSRVLGLKTL | LSIVLGLKTL | 1838 | 328 |
| HLA-B*40:01 | NELSRVLGL | NELSIVLGL | 170 | 31 |
| HLA-B*18:01 | NELSRVLGL | NELSIVLGL | 377 | 70 |
| HLA-B*40:02 | KKPNELSRVLGL | KKPNELSIVLGL | 931 | 197 |
| HLA-B*40:02 | KPNELSRVLGL | KPNELSIVLGL | 625 | 163 |
| HLA-B*44:02 | NELSRVLGL | NELSIVLGL | 435 | 124 |
| HLA-B*07:02 | KPNELSRVLGL | KPNELSIVLGL | 130 | 381 |
| HLA-B*07:02 | KKPNELSRVL | KKPNELSIVL | 38 | 98 |
| HLA-B*07:02 | KPNELSRVL | KPNELSIVL | 8 | 20 |
| HLA-B*40:02 | NELSRVLGL | NELSIVLGL | 64 | 26 |
| HLA-B*40:02 | NELSRVLGLKTL | NELSIVLGLKTL | 932 | 383 |
| HLA-B*15:03 | KKPNELSRVL | KKPNELSIVL | 573 | 246 |
| HLA-B*07:02 | KPNELSRVLG | KPNELSIVLG | 436 | 1007 |
| HLA-A*03:01 | ELSRVLGLK | ELSIVLGLK | 445 | 901 |
S1375Y
Reference YMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated YMPYFFTLLLQLCTFTRSTNSRIKAYMPTTIAKNTVKSVGKFCLEASFNYL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*16:01 | STNSRIKAS | STNSRIKAY | 5907 | 35 |
| HLA-C*03:02 | STNSRIKAS | STNSRIKAY | 13134 | 96 |
| HLA-A*11:01 | ASMPTTIAKN | AYMPTTIAKN | 41 | 5000 |
| HLA-A*26:01 | STNSRIKAS | STNSRIKAY | 22118 | 185 |
| HLA-B*15:01 | FTRSTNSRIKAS | FTRSTNSRIKAY | 22114 | 187 |
| HLA-C*12:03 | STNSRIKAS | STNSRIKAY | 7867 | 72 |
| HLA-A*30:02 | RSTNSRIKAS | RSTNSRIKAY | 8101 | 75 |
| HLA-A*30:02 | STNSRIKAS | STNSRIKAY | 8463 | 93 |
| HLA-B*15:03 | RSTNSRIKAS | RSTNSRIKAY | 21115 | 257 |
| HLA-B*15:03 | STNSRIKAS | STNSRIKAY | 20742 | 287 |
| HLA-C*16:01 | RSTNSRIKAS | RSTNSRIKAY | 18847 | 273 |
| HLA-B*15:01 | RSTNSRIKAS | RSTNSRIKAY | 22034 | 334 |
| HLA-B*15:01 | STNSRIKAS | STNSRIKAY | 19764 | 334 |
| HLA-C*12:02 | STNSRIKAS | STNSRIKAY | 13679 | 239 |
| HLA-A*11:01 | ASMPTTIAK | AYMPTTIAK | 4 | 224 |
| HLA-C*02:02 | STNSRIKAS | STNSRIKAY | 18992 | 384 |
| HLA-C*02:10 | STNSRIKAS | STNSRIKAY | 18992 | 384 |
| HLA-A*03:01 | ASMPTTIAK | AYMPTTIAK | 29 | 1177 |
| HLA-C*14:02 | ASMPTTIAKNTV | AYMPTTIAKNTV | 13809 | 458 |
| HLA-A*11:01 | SRIKASMPTTIAK | SRIKAYMPTTIAK | 19 | 536 |
| HLA-A*11:01 | ASMPTTIAKNTVK | AYMPTTIAKNTVK | 18 | 473 |
| HLA-A*03:02 | ASMPTTIAK | AYMPTTIAK | 21 | 533 |
| HLA-A*03:01 | ASMPTTIAKN | AYMPTTIAKN | 473 | 9304 |
| HLA-A*03:02 | ASMPTTIAKN | AYMPTTIAKN | 491 | 7386 |
| HLA-A*03:02 | ASMPTTIAKNTVK | AYMPTTIAKNTVK | 163 | 2214 |
| HLA-A*68:01 | ASMPTTIAK | AYMPTTIAK | 137 | 1756 |
| HLA-A*11:01 | IKASMPTTIAK | IKAYMPTTIAK | 34 | 358 |
| HLA-C*12:03 | KASMPTTIA | KAYMPTTIA | 1716 | 171 |
| HLA-A*03:01 | ASMPTTIAKNTVK | AYMPTTIAKNTVK | 232 | 2041 |
| HLA-B*15:03 | KASMPTTIA | KAYMPTTIA | 2254 | 297 |
| HLA-A*02:11 | SMPTTIAKNTV | YMPTTIAKNTV | 140 | 25 |
| HLA-A*02:06 | ASMPTTIAKNTV | AYMPTTIAKNTV | 313 | 1746 |
| HLA-C*03:02 | KASMPTTIA | KAYMPTTIA | 1880 | 352 |
| HLA-A*02:06 | SMPTTIAKNTV | YMPTTIAKNTV | 1144 | 216 |
| HLA-A*02:02 | SMPTTIAKNTV | YMPTTIAKNTV | 490 | 93 |
| HLA-A*02:11 | SMPTTIAKNTVKSV | YMPTTIAKNTVKSV | 444 | 88 |
| HLA-A*02:01 | SMPTTIAKNTV | YMPTTIAKNTV | 969 | 196 |
| HLA-A*03:01 | KASMPTTIAKNTVK | KAYMPTTIAKNTVK | 1490 | 312 |
| HLA-A*03:02 | IKASMPTTIAK | IKAYMPTTIAK | 237 | 1128 |
| HLA-A*30:01 | ASMPTTIAK | AYMPTTIAK | 14 | 59 |
| HLA-A*11:01 | SMPTTIAKNTVK | YMPTTIAKNTVK | 231 | 965 |
| HLA-A*02:02 | SMPTTIAKNTVKSV | YMPTTIAKNTVKSV | 792 | 194 |
| HLA-B*08:01 | NSRIKASM | NSRIKAYM | 168 | 668 |
| HLA-A*68:02 | STNSRIKASM | STNSRIKAYM | 1386 | 384 |
| HLA-A*03:01 | SMPTTIAKNTVK | YMPTTIAKNTVK | 473 | 1674 |
| HLA-A*30:01 | KASMPTTIA | KAYMPTTIA | 146 | 43 |
| HLA-C*16:01 | KASMPTTIA | KAYMPTTIA | 599 | 185 |
| HLA-A*30:01 | KASMPTTIAKNTVK | KAYMPTTIAKNTVK | 1443 | 473 |
| HLA-A*30:01 | ASMPTTIAKNTVK | AYMPTTIAKNTVK | 392 | 1113 |
| HLA-A*68:01 | SMPTTIAKNTVK | YMPTTIAKNTVK | 1151 | 439 |
| HLA-C*16:01 | KASMPTTI | KAYMPTTI | 922 | 369 |
| HLA-A*03:02 | SRIKASMPTTIAK | SRIKAYMPTTIAK | 154 | 362 |
| HLA-A*31:01 | KASMPTTIAK | KAYMPTTIAK | 209 | 92 |
| HLA-A*11:01 | KASMPTTIAKN | KAYMPTTIAKN | 411 | 926 |
| HLA-A*30:01 | STNSRIKASM | STNSRIKAYM | 1005 | 468 |
NSP6 protein
V84F
Reference FVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFK
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FVKHKHAFLCLFLLPSLATVAYFNMFYMPASWVMRIMTWLDMVDTSLSGFK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | ATVAYFNMV | ATVAYFNMF | 14 | 4642 |
| HLA-A*02:11 | ATVAYFNMV | ATVAYFNMF | 27 | 8909 |
| HLA-A*02:11 | SLATVAYFNMV | SLATVAYFNMF | 11 | 2388 |
| HLA-A*02:06 | ATVAYFNMV | ATVAYFNMF | 8 | 1324 |
| HLA-A*68:02 | LATVAYFNMV | LATVAYFNMF | 77 | 12129 |
| HLA-A*02:02 | ATVAYFNMV | ATVAYFNMF | 80 | 8774 |
| HLA-A*68:02 | SLATVAYFNMV | SLATVAYFNMF | 185 | 16271 |
| HLA-A*02:11 | PSLATVAYFNMV | PSLATVAYFNMF | 169 | 14714 |
| HLA-A*02:01 | SLATVAYFNMV | SLATVAYFNMF | 61 | 5227 |
| HLA-A*02:01 | ATVAYFNMV | ATVAYFNMF | 179 | 15321 |
| HLA-A*02:02 | SLATVAYFNMV | SLATVAYFNMF | 23 | 1576 |
| HLA-A*68:02 | MVYMPASWV | MFYMPASWV | 9 | 582 |
| HLA-B*15:03 | ATVAYFNMV | ATVAYFNMF | 4481 | 70 |
| HLA-B*15:01 | ATVAYFNMV | ATVAYFNMF | 7924 | 143 |
| HLA-A*02:06 | LATVAYFNMV | LATVAYFNMF | 176 | 8835 |
| HLA-A*02:02 | PSLATVAYFNMV | PSLATVAYFNMF | 223 | 9901 |
| HLA-A*02:06 | SLATVAYFNMV | SLATVAYFNMF | 159 | 6213 |
| HLA-A*02:06 | MVYMPASWV | MFYMPASWV | 64 | 2497 |
| HLA-B*15:03 | LATVAYFNMV | LATVAYFNMF | 12104 | 362 |
| HLA-A*02:11 | LLPSLATVAYFNMV | LLPSLATVAYFNMF | 494 | 16064 |
| HLA-A*68:02 | TVAYFNMV | TVAYFNMF | 417 | 13445 |
| HLA-A*23:01 | ATVAYFNMV | ATVAYFNMF | 12011 | 379 |
| HLA-B*35:01 | LPSLATVAYFNMV | LPSLATVAYFNMF | 4405 | 145 |
| HLA-A*68:02 | MVYMPASWVM | MFYMPASWVM | 197 | 5409 |
| HLA-C*15:02 | MVYMPASWV | MFYMPASWV | 384 | 10140 |
| HLA-C*15:02 | ATVAYFNMV | ATVAYFNMF | 185 | 3970 |
| HLA-C*14:02 | MVYMPASWV | MFYMPASWV | 1201 | 59 |
| HLA-A*02:11 | SLATVAYFNMVY | SLATVAYFNMFY | 352 | 6801 |
| HLA-A*02:11 | MVYMPASWV | MFYMPASWV | 18 | 331 |
| HLA-A*02:06 | MVYMPASWVM | MFYMPASWVM | 201 | 3696 |
| HLA-C*03:03 | MVYMPASWVM | MFYMPASWVM | 159 | 2293 |
| HLA-C*03:04 | MVYMPASWVM | MFYMPASWVM | 159 | 2293 |
| HLA-B*53:01 | LPSLATVAYFNMV | LPSLATVAYFNMF | 5314 | 377 |
| HLA-A*02:01 | MVYMPASWV | MFYMPASWV | 213 | 2797 |
| HLA-B*58:01 | LATVAYFNMV | LATVAYFNMF | 3556 | 279 |
| HLA-C*03:02 | ATVAYFNMV | ATVAYFNMF | 3190 | 257 |
| HLA-B*15:01 | MVYMPASWVM | MFYMPASWVM | 60 | 735 |
| HLA-A*68:01 | MVYMPASWVMR | MFYMPASWVMR | 16 | 195 |
| HLA-B*58:01 | ATVAYFNMV | ATVAYFNMF | 5612 | 474 |
| HLA-A*11:01 | MVYMPASWVMR | MFYMPASWVMR | 477 | 5248 |
| HLA-B*58:01 | MVYMPASWVM | MFYMPASWVM | 229 | 2515 |
| HLA-C*14:02 | MVYMPASW | MFYMPASW | 4093 | 374 |
| HLA-A*68:02 | MVYMPASWVMRI | MFYMPASWVMRI | 308 | 3084 |
| HLA-A*02:02 | MVYMPASWV | MFYMPASWV | 133 | 1327 |
| HLA-C*12:02 | VYMPASWVM | FYMPASWVM | 2252 | 228 |
| HLA-A*32:01 | YFNMVYMPASW | YFNMFYMPASW | 1440 | 148 |
| HLA-A*32:01 | FNMVYMPASW | FNMFYMPASW | 865 | 89 |
| HLA-A*02:02 | SLATVAYFNMVY | SLATVAYFNMFY | 467 | 4505 |
| HLA-B*35:01 | MVYMPASWVM | MFYMPASWVM | 55 | 462 |
| HLA-A*68:01 | MVYMPASWVMRI | MFYMPASWVMRI | 389 | 3256 |
| HLA-C*14:02 | MVYMPASWVMRIM | MFYMPASWVMRIM | 1696 | 204 |
| HLA-A*32:01 | NMVYMPASW | NMFYMPASW | 174 | 21 |
| HLA-C*03:02 | VYMPASWVM | FYMPASWVM | 451 | 55 |
| HLA-A*32:01 | ATVAYFNMV | ATVAYFNMF | 2266 | 281 |
| HLA-C*03:02 | MVYMPASWVM | MFYMPASWVM | 45 | 360 |
| HLA-A*03:01 | MVYMPASWVMR | MFYMPASWVMR | 471 | 3603 |
| HLA-C*12:03 | MVYMPASWV | MFYMPASWV | 198 | 1513 |
| HLA-C*03:03 | VYMPASWVM | FYMPASWVM | 2131 | 281 |
| HLA-C*03:04 | VYMPASWVM | FYMPASWVM | 2131 | 281 |
| HLA-A*02:11 | MVYMPASWVM | MFYMPASWVM | 279 | 2115 |
| HLA-B*35:01 | VYMPASWVM | FYMPASWVM | 1010 | 140 |
| HLA-A*29:02 | VAYFNMVYM | VAYFNMFYM | 1560 | 231 |
| HLA-A*03:02 | TVAYFNMVY | TVAYFNMFY | 578 | 87 |
| HLA-B*15:01 | SLATVAYFNMVY | SLATVAYFNMFY | 148 | 905 |
| HLA-B*15:03 | SLATVAYFNMVY | SLATVAYFNMFY | 419 | 2516 |
| HLA-A*03:02 | ATVAYFNMVY | ATVAYFNMFY | 657 | 113 |
| HLA-B*15:03 | LATVAYFNMVY | LATVAYFNMFY | 328 | 1903 |
| HLA-A*32:01 | AYFNMVYMPASW | AYFNMFYMPASW | 1285 | 226 |
| HLA-A*02:01 | NMVYMPASWV | NMFYMPASWV | 357 | 64 |
| HLA-C*12:03 | VYMPASWVM | FYMPASWVM | 786 | 141 |
| HLA-A*02:02 | MVYMPASWVM | MFYMPASWVM | 466 | 2574 |
| HLA-B*15:03 | VAYFNMVY | VAYFNMFY | 60 | 313 |
| HLA-A*29:02 | VYMPASWVM | FYMPASWVM | 556 | 107 |
| HLA-A*68:01 | VYMPASWVMR | FYMPASWVMR | 452 | 88 |
| HLA-A*68:01 | ATVAYFNMVY | ATVAYFNMFY | 471 | 94 |
| HLA-A*32:01 | VAYFNMVYMPASW | VAYFNMFYMPASW | 2410 | 485 |
| HLA-B*15:03 | ATVAYFNMVY | ATVAYFNMFY | 103 | 507 |
| HLA-B*15:01 | ATVAYFNMVY | ATVAYFNMFY | 43 | 207 |
| HLA-A*02:11 | NMVYMPASWV | NMFYMPASWV | 72 | 15 |
| HLA-A*02:06 | VAYFNMVYM | VAYFNMFYM | 587 | 123 |
| HLA-B*15:03 | TVAYFNMVY | TVAYFNMFY | 21 | 100 |
| HLA-A*11:01 | TVAYFNMVY | TVAYFNMFY | 85 | 18 |
| HLA-B*35:01 | TVAYFNMVY | TVAYFNMFY | 21 | 99 |
| HLA-A*02:11 | NMVYMPASWVMRI | NMFYMPASWVMRI | 471 | 102 |
| HLA-A*32:01 | MVYMPASWVM | MFYMPASWVM | 441 | 2035 |
| HLA-A*23:01 | NMVYMPASW | NMFYMPASW | 1303 | 292 |
| HLA-B*15:03 | NMVYMPASW | NMFYMPASW | 279 | 63 |
| HLA-A*02:11 | VAYFNMVYM | VAYFNMFYM | 515 | 117 |
| HLA-C*03:02 | LATVAYFNMVY | LATVAYFNMFY | 338 | 1466 |
| HLA-C*16:01 | MVYMPASWVM | MFYMPASWVM | 250 | 1079 |
| HLA-A*11:01 | SLATVAYFNMVY | SLATVAYFNMFY | 1317 | 313 |
| HLA-C*12:02 | MVYMPASWVM | MFYMPASWVM | 293 | 1231 |
| HLA-A*68:01 | TVAYFNMVY | TVAYFNMFY | 46 | 11 |
| HLA-B*58:01 | MVYMPASW | MFYMPASW | 112 | 464 |
| HLA-A*03:01 | TVAYFNMVY | TVAYFNMFY | 563 | 136 |
| HLA-A*29:02 | VAYFNMVY | VAYFNMFY | 900 | 223 |
| HLA-C*03:02 | TVAYFNMVY | TVAYFNMFY | 36 | 145 |
| HLA-C*06:02 | VYMPASWVM | FYMPASWVM | 1753 | 440 |
| HLA-B*58:01 | VAYFNMVYM | VAYFNMFYM | 878 | 222 |
| HLA-B*15:01 | TVAYFNMVY | TVAYFNMFY | 40 | 156 |
| HLA-B*57:01 | MVYMPASW | MFYMPASW | 257 | 995 |
| HLA-C*14:02 | VYMPASWVMRIM | FYMPASWVMRIM | 58 | 15 |
| HLA-A*03:01 | ATVAYFNMVY | ATVAYFNMFY | 451 | 118 |
| HLA-A*02:02 | NMVYMPASWV | NMFYMPASWV | 103 | 27 |
| HLA-A*29:02 | TVAYFNMVYM | TVAYFNMFYM | 524 | 138 |
| HLA-C*14:02 | VYMPASWVMR | FYMPASWVMR | 181 | 49 |
| HLA-C*07:01 | VYMPASWVM | FYMPASWVM | 372 | 104 |
| HLA-A*03:02 | MVYMPASWVMR | MFYMPASWVMR | 484 | 1731 |
| HLA-C*14:02 | VYMPASWV | FYMPASWV | 274 | 77 |
| HLA-C*16:01 | VYMPASWVM | FYMPASWVM | 309 | 90 |
| HLA-C*14:02 | VYMPASWVMRI | FYMPASWVMRI | 571 | 167 |
| HLA-A*33:03 | VYMPASWVMR | FYMPASWVMR | 41 | 12 |
| HLA-A*11:01 | ATVAYFNMVY | ATVAYFNMFY | 68 | 20 |
| HLA-B*15:03 | FNMVYMPASW | FNMFYMPASW | 501 | 148 |
| HLA-A*03:01 | SLATVAYFNMVY | SLATVAYFNMFY | 1608 | 476 |
| HLA-C*07:02 | VYMPASWVMRIM | FYMPASWVMRIM | 1141 | 340 |
| HLA-C*03:02 | VAYFNMVY | VAYFNMFY | 54 | 180 |
| HLA-C*12:03 | MVYMPASWVM | MFYMPASWVM | 344 | 1094 |
| HLA-A*29:02 | LATVAYFNMVY | LATVAYFNMFY | 308 | 101 |
| HLA-C*03:02 | ATVAYFNMVY | ATVAYFNMFY | 432 | 1289 |
| HLA-A*29:02 | MVYMPASWVM | MFYMPASWVM | 296 | 100 |
| HLA-A*02:11 | TVAYFNMVYM | TVAYFNMFYM | 638 | 216 |
| HLA-A*03:02 | SLATVAYFNMVY | SLATVAYFNMFY | 1137 | 390 |
| HLA-A*68:02 | VAYFNMVYM | VAYFNMFYM | 591 | 203 |
| HLA-B*57:01 | NMVYMPASW | NMFYMPASW | 915 | 320 |
| HLA-C*16:01 | VAYFNMVY | VAYFNMFY | 54 | 154 |
| HLA-A*33:03 | NMVYMPASWVMR | NMFYMPASWVMR | 72 | 26 |
| HLA-A*02:02 | NMVYMPASWVMRI | NMFYMPASWVMRI | 1062 | 384 |
| HLA-A*23:01 | YFNMVYMPASW | YFNMFYMPASW | 350 | 127 |
| HLA-A*01:01 | ATVAYFNMVY | ATVAYFNMFY | 189 | 70 |
| HLA-A*29:02 | LPSLATVAYFNMVY | LPSLATVAYFNMFY | 1294 | 489 |
| HLA-A*01:01 | TVAYFNMVY | TVAYFNMFY | 360 | 137 |
| HLA-C*16:01 | TVAYFNMVY | TVAYFNMFY | 100 | 258 |
| HLA-B*15:03 | NMVYMPASWVM | NMFYMPASWVM | 437 | 170 |
| HLA-C*12:03 | VAYFNMVY | VAYFNMFY | 163 | 407 |
| HLA-A*29:02 | ATVAYFNMVY | ATVAYFNMFY | 27 | 11 |
| HLA-B*15:01 | NMVYMPASW | NMFYMPASW | 1049 | 432 |
| HLA-B*58:01 | NMVYMPASW | NMFYMPASW | 167 | 70 |
| HLA-B*53:01 | NMVYMPASW | NMFYMPASW | 333 | 142 |
| HLA-A*02:06 | NMVYMPASWV | NMFYMPASWV | 796 | 340 |
| HLA-A*02:11 | MVYMPASWVMRI | MFYMPASWVMRI | 176 | 411 |
| HLA-A*24:02 | MVYMPASWVMRI | MFYMPASWVMRI | 213 | 494 |
| HLA-B*15:01 | FNMVYMPASW | FNMFYMPASW | 597 | 261 |
| HLA-C*12:02 | VAYFNMVY | VAYFNMFY | 373 | 825 |
| HLA-A*02:11 | SLATVAYFNMVYM | SLATVAYFNMFYM | 152 | 69 |
| HLA-C*12:02 | TVAYFNMVY | TVAYFNMFY | 150 | 319 |
| HLA-A*68:01 | TVAYFNMVYM | TVAYFNMFYM | 280 | 132 |
| HLA-A*31:01 | NMVYMPASWVMR | NMFYMPASWVMR | 122 | 58 |
| HLA-A*02:06 | TVAYFNMVYM | TVAYFNMFYM | 445 | 213 |
| HLA-B*58:01 | YFNMVYMPASW | YFNMFYMPASW | 507 | 249 |
| HLA-C*14:02 | VYMPASWVM | FYMPASWVM | 4 | 2 |
| HLA-A*29:02 | TVAYFNMVY | TVAYFNMFY | 6 | 3 |
| HLA-C*14:02 | MVYMPASWVM | MFYMPASWVM | 18 | 9 |
| HLA-C*07:02 | VYMPASWVM | FYMPASWVM | 26 | 13 |
| HLA-B*15:03 | MVYMPASWVM | MFYMPASWVM | 42 | 84 |
NSP10 protein
T111I
Reference PKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREP
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PKGFCDLKGKYVQIPTTCANDPVGFILKNTVCTVCGMWKGYGCSCDQLREP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*17:01 | CANDPVGFT | CANDPVGFI | 10662 | 427 |
| HLA-C*12:03 | CANDPVGFT | CANDPVGFI | 4921 | 198 |
| HLA-B*57:01 | FTLKNTVCTVCGMW | FILKNTVCTVCGMW | 378 | 6985 |
| HLA-B*58:01 | FTLKNTVCTVCGMW | FILKNTVCTVCGMW | 345 | 5931 |
| HLA-A*68:02 | FTLKNTVCTV | FILKNTVCTV | 50 | 601 |
| HLA-A*68:02 | CANDPVGFT | CANDPVGFI | 2369 | 238 |
| HLA-A*02:11 | FTLKNTVCT | FILKNTVCT | 1421 | 327 |
| HLA-C*12:03 | FTLKNTVCTV | FILKNTVCTV | 447 | 1789 |
| HLA-A*02:01 | TLKNTVCTV | ILKNTVCTV | 217 | 71 |
| HLA-A*02:01 | FTLKNTVCTV | FILKNTVCTV | 45 | 16 |
| HLA-A*02:06 | TLKNTVCTV | ILKNTVCTV | 511 | 230 |
| HLA-C*16:01 | CANDPVGFTL | CANDPVGFIL | 496 | 1039 |
| HLA-A*02:11 | TLKNTVCTV | ILKNTVCTV | 14 | 7 |
NSP12 protein
S229N
Reference VLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAE
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated VLTLDNQDLNGNWYDFGDFIQTTPGNGVPVVDSYYSLLMPILTLTRALTAE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | SGVPVVDSY | NGVPVVDSY | 229 | 1058 |
| HLA-B*15:01 | SGVPVVDSYY | NGVPVVDSYY | 491 | 2267 |
| HLA-A*30:02 | SGVPVVDSY | NGVPVVDSY | 332 | 1398 |
| HLA-A*30:02 | SGVPVVDSYY | NGVPVVDSYY | 125 | 478 |
| HLA-A*30:02 | GSGVPVVDSY | GNGVPVVDSY | 403 | 1304 |
| HLA-C*03:02 | SGVPVVDSY | NGVPVVDSY | 253 | 762 |
| HLA-C*16:01 | SGVPVVDSY | NGVPVVDSY | 394 | 1089 |
| HLA-B*35:01 | SGVPVVDSY | NGVPVVDSY | 334 | 126 |
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |
NSP13 protein
P77L
Reference APGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDN
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Mutated APGCDVTDVTQLYLGGMSYYCKSHKLPISFPLCANGQVFGLYKNTCVGSDN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | KPPISFPL | KLPISFPL | 18334 | 25 |
| HLA-C*14:02 | SYYCKSHKP | SYYCKSHKL | 4966 | 8 |
| HLA-A*02:11 | YLGGMSYYCKSHKP | YLGGMSYYCKSHKL | 7927 | 24 |
| HLA-A*02:11 | KPPISFPLCA | KLPISFPLCA | 20510 | 68 |
| HLA-A*02:01 | KPPISFPL | KLPISFPL | 25214 | 96 |
| HLA-A*02:02 | KPPISFPL | KLPISFPL | 22090 | 87 |
| HLA-A*02:01 | KPPISFPLCA | KLPISFPLCA | 25382 | 129 |
| HLA-A*02:02 | KPPISFPLCA | KLPISFPLCA | 25317 | 138 |
| HLA-A*24:02 | SYYCKSHKP | SYYCKSHKL | 9919 | 55 |
| HLA-C*07:02 | SYYCKSHKP | SYYCKSHKL | 16095 | 92 |
| HLA-A*23:01 | SYYCKSHKP | SYYCKSHKL | 9507 | 55 |
| HLA-A*02:11 | KPPISFPLC | KLPISFPLC | 17315 | 110 |
| HLA-A*02:02 | YLGGMSYYCKSHKP | YLGGMSYYCKSHKL | 6432 | 43 |
| HLA-C*14:02 | MSYYCKSHKP | MSYYCKSHKL | 16012 | 125 |
| HLA-B*42:01 | KPPISFPL | KLPISFPL | 62 | 7080 |
| HLA-B*07:02 | KPPISFPL | KLPISFPL | 193 | 17835 |
| HLA-B*35:01 | PPISFPLCA | LPISFPLCA | 14708 | 181 |
| HLA-C*16:01 | MSYYCKSHKP | MSYYCKSHKL | 18091 | 229 |
| HLA-A*02:01 | YLGGMSYYCKSHKP | YLGGMSYYCKSHKL | 11245 | 157 |
| HLA-A*23:01 | MSYYCKSHKP | MSYYCKSHKL | 14220 | 233 |
| HLA-A*02:06 | KPPISFPL | KLPISFPL | 18292 | 316 |
| HLA-B*35:01 | PPISFPLCANGQVF | LPISFPLCANGQVF | 12721 | 244 |
| HLA-A*02:06 | KPPISFPLCA | KLPISFPLCA | 18606 | 359 |
| HLA-C*14:02 | YYCKSHKP | YYCKSHKL | 7930 | 158 |
| HLA-A*02:11 | GMSYYCKSHKP | GMSYYCKSHKL | 18709 | 374 |
| HLA-A*02:06 | KPPISFPLC | KLPISFPLC | 17150 | 345 |
| HLA-A*24:02 | MSYYCKSHKP | MSYYCKSHKL | 16184 | 345 |
| HLA-A*02:11 | KPPISFPLCANGQV | KLPISFPLCANGQV | 22321 | 498 |
| HLA-B*53:01 | PPISFPLCANGQVF | LPISFPLCANGQVF | 10632 | 240 |
| HLA-A*02:02 | GMSYYCKSHKP | GMSYYCKSHKL | 14723 | 382 |
| HLA-B*42:01 | PPISFPLCA | LPISFPLCA | 9497 | 287 |
| HLA-A*68:02 | MSYYCKSHKP | MSYYCKSHKL | 6598 | 220 |
| HLA-B*15:03 | HKPPISFPL | HKLPISFPL | 509 | 24 |
| HLA-B*58:01 | MSYYCKSHKP | MSYYCKSHKL | 3227 | 360 |
| HLA-B*38:01 | HKPPISFPL | HKLPISFPL | 2540 | 331 |
| HLA-B*38:01 | SHKPPISFPL | SHKLPISFPL | 639 | 119 |
| HLA-B*57:01 | KSHKPPISFPL | KSHKLPISFPL | 1359 | 353 |
| HLA-B*58:01 | KSHKPPISFPL | KSHKLPISFPL | 716 | 187 |
| HLA-B*08:01 | YCKSHKPPI | YCKSHKLPI | 555 | 175 |
| HLA-B*15:03 | SHKPPISFPL | SHKLPISFPL | 858 | 274 |
| HLA-A*24:02 | YYCKSHKPPI | YYCKSHKLPI | 429 | 143 |
| HLA-A*23:01 | YYCKSHKPPI | YYCKSHKLPI | 408 | 150 |
| HLA-C*14:02 | YYCKSHKPPISF | YYCKSHKLPISF | 92 | 221 |
| HLA-C*03:02 | YCKSHKPPISF | YCKSHKLPISF | 393 | 858 |
| HLA-A*32:01 | KSHKPPISF | KSHKLPISF | 89 | 42 |
| HLA-C*14:02 | YYCKSHKPPI | YYCKSHKLPI | 347 | 171 |
L455M
Reference KTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVI
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Mutated KTIGPDMFLGTCRRCPAEIVDTVSAMVYDNKLKAHKDKSAQCFKMFYKGVI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | LVYDNKLKA | MVYDNKLKA | 2250 | 153 |
| HLA-B*35:01 | EIVDTVSAL | EIVDTVSAM | 1875 | 148 |
| HLA-A*68:01 | LVYDNKLKAHK | MVYDNKLKAHK | 741 | 71 |
| HLA-B*35:01 | CPAEIVDTVSAL | CPAEIVDTVSAM | 1113 | 115 |
| HLA-A*68:01 | LVYDNKLK | MVYDNKLK | 4005 | 439 |
| HLA-A*26:01 | AEIVDTVSAL | AEIVDTVSAM | 3675 | 421 |
| HLA-A*68:01 | LVYDNKLKAHKDK | MVYDNKLKAHKDK | 3316 | 382 |
| HLA-C*03:03 | SALVYDNKL | SAMVYDNKL | 288 | 48 |
| HLA-C*03:04 | SALVYDNKL | SAMVYDNKL | 288 | 48 |
| HLA-C*16:01 | SALVYDNKL | SAMVYDNKL | 253 | 44 |
| HLA-A*26:01 | EIVDTVSAL | EIVDTVSAM | 27 | 5 |
| HLA-B*15:01 | AEIVDTVSAL | AEIVDTVSAM | 1539 | 305 |
| HLA-C*03:02 | SALVYDNKL | SAMVYDNKL | 1112 | 225 |
| HLA-A*68:01 | SALVYDNKLK | SAMVYDNKLK | 967 | 200 |
| HLA-B*15:03 | AEIVDTVSAL | AEIVDTVSAM | 1192 | 250 |
| HLA-C*12:03 | SALVYDNKL | SAMVYDNKL | 1434 | 316 |
| HLA-A*02:02 | AEIVDTVSAL | AEIVDTVSAM | 428 | 1691 |
| HLA-A*11:01 | SALVYDNKLK | SAMVYDNKLK | 422 | 121 |
| HLA-B*40:01 | AEIVDTVSAL | AEIVDTVSAM | 19 | 62 |
| HLA-C*17:01 | SALVYDNKL | SAMVYDNKL | 1522 | 470 |
| HLA-A*02:11 | LVYDNKLKA | MVYDNKLKA | 882 | 287 |
| HLA-C*12:03 | LVYDNKLKA | MVYDNKLKA | 967 | 323 |
| HLA-A*03:02 | LVYDNKLKAHK | MVYDNKLKAHK | 718 | 259 |
| HLA-A*68:02 | EIVDTVSAL | EIVDTVSAM | 44 | 120 |
| HLA-A*11:01 | LVYDNKLKAHKDK | MVYDNKLKAHKDK | 771 | 295 |
| HLA-A*11:01 | LVYDNKLKAHK | MVYDNKLKAHK | 179 | 72 |
| HLA-B*42:01 | CPAEIVDTVSAL | CPAEIVDTVSAM | 186 | 415 |
| HLA-A*03:02 | SALVYDNKLK | SAMVYDNKLK | 1014 | 457 |
| HLA-A*03:01 | LVYDNKLKAHKDK | MVYDNKLKAHKDK | 812 | 366 |
| HLA-A*03:01 | LVYDNKLKAHK | MVYDNKLKAHK | 235 | 111 |
| HLA-A*25:01 | EIVDTVSAL | EIVDTVSAM | 66 | 32 |