Summary
This strain contains 11 protein-level mutations:
- 4 mutations in Spike protein: S13I, W152C, L452R, D614G
- 1 mutation in N protein: T205I
- 2 mutations in NS3 protein: Q57H, A99V
- 1 mutation in NS9c protein: L52F
- 1 mutation in NSP2 protein: T85I
- 1 mutation in NSP12 protein: P323L
- 1 mutation in NSP13 protein: D260Y
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of interactions between HLA-DRB1*15:02 and peptides affected by the mutations
Weaker binding was found for 1 epitope, while stronger binding was found for 86 entries. There are 92 tight binders for the HLA-DRB1*15:02 in the reference immunopeptidome (IC50 affinity ≤ 50 nM); 0 of them became non-tight binders (0.0%) and 0 novel tight binders appeared (0.0%). To avoid possible divisions by zero, we used + 1 regularization term in denominators when calculating percentages.
Spike protein
L452R
Reference DDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGS
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated DDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:02 | KVGGNYNYLYRLFRK | KVGGNYNYRYRLFRK | 415 | 920 |
NSP2 protein
T85I
Reference WYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEK
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated WYTERSEKSYELQTPFEIKLAKKFDIFNGECPNFVFPLNSIIKTIQPRVEK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:02 | PFEIKLAKKFDTFNGECPNF | PFEIKLAKKFDIFNGECPNF | 1054 | 425 |
| HLA-DRB1*15:02 | EIKLAKKFDTFNGECPNFVF | EIKLAKKFDIFNGECPNFVF | 1177 | 478 |
| HLA-DRB1*15:02 | FEIKLAKKFDTFNGECPNFV | FEIKLAKKFDIFNGECPNFV | 991 | 412 |
| HLA-DRB1*15:02 | TPFEIKLAKKFDTFN | TPFEIKLAKKFDIFN | 1002 | 417 |
| HLA-DRB1*15:02 | TPFEIKLAKKFDTFNGECPN | TPFEIKLAKKFDIFNGECPN | 1002 | 426 |
| HLA-DRB1*15:02 | QTPFEIKLAKKFDTF | QTPFEIKLAKKFDIF | 980 | 420 |
| HLA-DRB1*15:02 | PFEIKLAKKFDTFNG | PFEIKLAKKFDIFNG | 1097 | 476 |
| HLA-DRB1*15:02 | QTPFEIKLAKKFDTFNGECP | QTPFEIKLAKKFDIFNGECP | 950 | 422 |
| HLA-DRB1*15:02 | LQTPFEIKLAKKFDTFNGEC | LQTPFEIKLAKKFDIFNGEC | 935 | 451 |
| HLA-DRB1*15:02 | ELQTPFEIKLAKKFDTFNGE | ELQTPFEIKLAKKFDIFNGE | 901 | 435 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:02 | FSTVFPPTSFGPLVRK | FSTVFPLTSFGPLVRK | 4257 | 498 |
| HLA-DRB1*15:02 | STVFPPTSFGPLVRKI | STVFPLTSFGPLVRKI | 3779 | 444 |
| HLA-DRB1*15:02 | STVFPPTSFGPLVRK | STVFPLTSFGPLVRK | 2712 | 325 |
| HLA-DRB1*15:02 | TVFPPTSFGPLVRKI | TVFPLTSFGPLVRKI | 2258 | 303 |
| HLA-DRB1*15:02 | FSTVFPPTSFGPLVR | FSTVFPLTSFGPLVR | 2542 | 347 |
| HLA-DRB1*15:02 | FSTVFPPTSFGPLVRKI | FSTVFPLTSFGPLVRKI | 2916 | 408 |
| HLA-DRB1*15:02 | TVFPPTSFGPLVRKIF | TVFPLTSFGPLVRKIF | 2890 | 451 |
| HLA-DRB1*15:02 | STVFPPTSFGPLVRKIF | STVFPLTSFGPLVRKIF | 2417 | 392 |
| HLA-DRB1*15:02 | VFPPTSFGPLVRKIF | VFPLTSFGPLVRKIF | 1702 | 312 |
| HLA-DRB1*15:02 | LFSTVFPPTSFGPLVRK | LFSTVFPLTSFGPLVRK | 2483 | 464 |
| HLA-DRB1*15:02 | TVFPPTSFGPLVRKIFV | TVFPLTSFGPLVRKIFV | 2217 | 429 |
| HLA-DRB1*15:02 | FSTVFPPTSFGPLVRKIFVD | FSTVFPLTSFGPLVRKIFVD | 1680 | 360 |
| HLA-DRB1*15:02 | STVFPPTSFGPLVRKIFVDG | STVFPLTSFGPLVRKIFVDG | 1794 | 396 |
| HLA-DRB1*15:02 | LFSTVFPPTSFGPLVRKIF | LFSTVFPLTSFGPLVRKIF | 2232 | 497 |
| HLA-DRB1*15:02 | LFSTVFPPTSFGPLVRKIFV | LFSTVFPLTSFGPLVRKIFV | 1344 | 334 |
| HLA-DRB1*15:02 | VLFSTVFPPTSFGPLVRKI | VLFSTVFPLTSFGPLVRKI | 1946 | 497 |
| HLA-DRB1*15:02 | FPPTSFGPLVRKIFV | FPLTSFGPLVRKIFV | 1770 | 453 |
| HLA-DRB1*15:02 | VLFSTVFPPTSFGPLVR | VLFSTVFPLTSFGPLVR | 1703 | 471 |
| HLA-DRB1*15:02 | NVLFSTVFPPTSFGPLVRK | NVLFSTVFPLTSFGPLVRK | 1672 | 489 |
| HLA-DRB1*15:02 | VLFSTVFPPTSFGPLVRKIF | VLFSTVFPLTSFGPLVRKIF | 1039 | 304 |
| HLA-DRB1*15:02 | TVFPPTSFGPLVRKIFVDGV | TVFPLTSFGPLVRKIFVDGV | 1430 | 426 |
| HLA-DRB1*15:02 | NVLFSTVFPPTSFGPLVRKI | NVLFSTVFPLTSFGPLVRKI | 968 | 301 |
| HLA-DRB1*15:02 | HCANFNVLFSTVFPPTS | HCANFNVLFSTVFPLTS | 707 | 261 |
| HLA-DRB1*15:02 | CANFNVLFSTVFPPTS | CANFNVLFSTVFPLTS | 742 | 274 |
| HLA-DRB1*15:02 | NVLFSTVFPPTSFGP | NVLFSTVFPLTSFGP | 799 | 296 |
| HLA-DRB1*15:02 | CANFNVLFSTVFPPT | CANFNVLFSTVFPLT | 427 | 161 |
| HLA-DRB1*15:02 | HCANFNVLFSTVFPP | HCANFNVLFSTVFPL | 646 | 244 |
| HLA-DRB1*15:02 | ANFNVLFSTVFPPTS | ANFNVLFSTVFPLTS | 325 | 126 |
| HLA-DRB1*15:02 | FNVLFSTVFPPTSFGPLVRK | FNVLFSTVFPLTSFGPLVRK | 796 | 309 |
| HLA-DRB1*15:02 | NFNVLFSTVFPPTSF | NFNVLFSTVFPLTSF | 328 | 128 |
| HLA-DRB1*15:02 | FNVLFSTVFPPTSFGPLVR | FNVLFSTVFPLTSFGPLVR | 1261 | 499 |
| HLA-DRB1*15:02 | NFNVLFSTVFPPTSFG | NFNVLFSTVFPLTSFG | 715 | 296 |
| HLA-DRB1*15:02 | NFNVLFSTVFPPTSFGP | NFNVLFSTVFPLTSFGP | 708 | 299 |
| HLA-DRB1*15:02 | HCANFNVLFSTVFPPT | HCANFNVLFSTVFPLT | 809 | 345 |
| HLA-DRB1*15:02 | LHCANFNVLFSTVFPPT | LHCANFNVLFSTVFPLT | 729 | 311 |
| HLA-DRB1*15:02 | CANFNVLFSTVFPPTSF | CANFNVLFSTVFPLTSF | 585 | 250 |
| HLA-DRB1*15:02 | LHCANFNVLFSTVFPP | LHCANFNVLFSTVFPL | 1052 | 453 |
| HLA-DRB1*15:02 | NFNVLFSTVFPPTSFGPLVR | NFNVLFSTVFPLTSFGPLVR | 653 | 283 |
| HLA-DRB1*15:02 | FNVLFSTVFPPTSFG | FNVLFSTVFPLTSFG | 481 | 212 |
| HLA-DRB1*15:02 | ANFNVLFSTVFPPTSFG | ANFNVLFSTVFPLTSFG | 576 | 264 |
| HLA-DRB1*15:02 | ANFNVLFSTVFPPTSF | ANFNVLFSTVFPLTSF | 558 | 258 |
| HLA-DRB1*15:02 | FNVLFSTVFPPTSFGP | FNVLFSTVFPLTSFGP | 1029 | 478 |
| HLA-DRB1*15:02 | FNVLFSTVFPPTSFGPL | FNVLFSTVFPLTSFGPL | 944 | 439 |
NSP13 protein
D260Y
Reference LSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPG
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LSAPTLVPQEHYVRITGLYPTLNISYEFSSNVANYQKVGMQKYSTLQGPPG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-DRB1*15:02 | TLNISDEFSSNVANY | TLNISYEFSSNVANY | 3200 | 280 |
| HLA-DRB1*15:02 | PTLNISDEFSSNVANY | PTLNISYEFSSNVANY | 5000 | 474 |
| HLA-DRB1*15:02 | TGLYPTLNISDEFSSNVANY | TGLYPTLNISYEFSSNVANY | 2004 | 214 |
| HLA-DRB1*15:02 | PTLNISDEFSSNVAN | PTLNISYEFSSNVAN | 3655 | 413 |
| HLA-DRB1*15:02 | PTLNISDEFSSNVANYQ | PTLNISYEFSSNVANYQ | 3641 | 476 |
| HLA-DRB1*15:02 | GLYPTLNISDEFSSNVANYQ | GLYPTLNISYEFSSNVANYQ | 1552 | 205 |
| HLA-DRB1*15:02 | LNISDEFSSNVANYQ | LNISYEFSSNVANYQ | 2342 | 311 |
| HLA-DRB1*15:02 | TLNISDEFSSNVANYQ | TLNISYEFSSNVANYQ | 2981 | 422 |
| HLA-DRB1*15:02 | ITGLYPTLNISDEFSSNVAN | ITGLYPTLNISYEFSSNVAN | 1754 | 249 |
| HLA-DRB1*15:02 | LYPTLNISDEFSSNVANYQK | LYPTLNISYEFSSNVANYQK | 1339 | 197 |
| HLA-DRB1*15:02 | TLNISDEFSSNVANYQK | TLNISYEFSSNVANYQK | 2862 | 442 |
| HLA-DRB1*15:02 | YPTLNISDEFSSNVANYQK | YPTLNISYEFSSNVANYQK | 2768 | 452 |
| HLA-DRB1*15:02 | LNISDEFSSNVANYQK | LNISYEFSSNVANYQK | 2291 | 425 |
| HLA-DRB1*15:02 | YPTLNISDEFSSNVANYQKV | YPTLNISYEFSSNVANYQKV | 984 | 185 |
| HLA-DRB1*15:02 | PTLNISDEFSSNVANYQKVG | PTLNISYEFSSNVANYQKVG | 1061 | 201 |
| HLA-DRB1*15:02 | RITGLYPTLNISDEFSSNVA | RITGLYPTLNISYEFSSNVA | 1346 | 261 |
| HLA-DRB1*15:02 | NISDEFSSNVANYQK | NISYEFSSNVANYQK | 1839 | 374 |
| HLA-DRB1*15:02 | PTLNISDEFSSNVANYQKV | PTLNISYEFSSNVANYQKV | 1982 | 413 |
| HLA-DRB1*15:02 | TLNISDEFSSNVANYQKVG | TLNISYEFSSNVANYQKVG | 2068 | 457 |
| HLA-DRB1*15:02 | TLNISDEFSSNVANYQKVGM | TLNISYEFSSNVANYQKVGM | 957 | 219 |
| HLA-DRB1*15:02 | LNISDEFSSNVANYQKV | LNISYEFSSNVANYQKV | 1979 | 487 |
| HLA-DRB1*15:02 | ITGLYPTLNISDEFS | ITGLYPTLNISYEFS | 1589 | 406 |
| HLA-DRB1*15:02 | LNISDEFSSNVANYQKVGMQ | LNISYEFSSNVANYQKVGMQ | 983 | 279 |
| HLA-DRB1*15:02 | VRITGLYPTLNISDEFSSNV | VRITGLYPTLNISYEFSSNV | 942 | 283 |
| HLA-DRB1*15:02 | NISDEFSSNVANYQKV | NISYEFSSNVANYQKV | 1533 | 481 |
| HLA-DRB1*15:02 | RITGLYPTLNISDEF | RITGLYPTLNISYEF | 1104 | 350 |
| HLA-DRB1*15:02 | ISDEFSSNVANYQKV | ISYEFSSNVANYQKV | 1153 | 427 |
| HLA-DRB1*15:02 | NISDEFSSNVANYQKVGMQK | NISYEFSSNVANYQKVGMQK | 966 | 364 |
| HLA-DRB1*15:02 | YVRITGLYPTLNISDEFSSN | YVRITGLYPTLNISYEFSSN | 686 | 288 |
| HLA-DRB1*15:02 | VRITGLYPTLNISDE | VRITGLYPTLNISYE | 770 | 331 |
| HLA-DRB1*15:02 | YVRITGLYPTLNISDEF | YVRITGLYPTLNISYEF | 961 | 468 |
| HLA-DRB1*15:02 | HYVRITGLYPTLNISDEFSS | HYVRITGLYPTLNISYEFSS | 519 | 254 |
| HLA-DRB1*15:02 | HYVRITGLYPTLNISDEFS | HYVRITGLYPTLNISYEFS | 943 | 471 |