Summary
This strain contains 20 protein-level mutations:
- 8 mutations in Spike protein: Q52R, A67V, HV 69-70 deletion, Y144 deletion, E484K, D614G, Q677H, F888L
- 4 mutations in N protein: S2 deletion, D3Y, A12G, T205I
- 1 mutation in M protein: I82T
- 1 mutation in E protein: L21F
- 1 mutation in NS6 protein: F2 deletion
- 1 mutation in NS9b protein: H9D
- 1 mutation in NS9c protein: L52F
- 1 mutation in NSP3 protein: T1189I
- 1 mutation in NSP6 protein: SGF 106-108 deletion
- 1 mutation in NSP12 protein: P323F
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
Q52R
Reference AYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTK
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Mutated AYTNSFTRGVYYPDKVFRSSVLHSTRDLFLPFFSNVTWFHVI‑‑SGTNGTK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*31:01 | RSSVLHSTQ | RSSVLHSTR | 7157 | 7 |
| HLA-A*31:01 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 11964 | 15 |
| HLA-A*68:01 | FRSSVLHSTQ | FRSSVLHSTR | 9279 | 25 |
| HLA-A*31:01 | FRSSVLHSTQ | FRSSVLHSTR | 21936 | 75 |
| HLA-A*31:01 | VFRSSVLHSTQ | VFRSSVLHSTR | 14990 | 60 |
| HLA-A*33:03 | FRSSVLHSTQ | FRSSVLHSTR | 23602 | 97 |
| HLA-A*31:01 | DKVFRSSVLHSTQ | DKVFRSSVLHSTR | 29057 | 192 |
| HLA-A*33:03 | RSSVLHSTQ | RSSVLHSTR | 24802 | 229 |
| HLA-A*31:01 | RSSVLHSTQD | RSSVLHSTRD | 27209 | 280 |
| HLA-A*33:03 | VFRSSVLHSTQ | VFRSSVLHSTR | 23512 | 265 |
| HLA-A*68:01 | RSSVLHSTQ | RSSVLHSTR | 18086 | 261 |
| HLA-A*74:01 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 17684 | 281 |
| HLA-A*33:03 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 29777 | 500 |
| HLA-A*02:06 | TQDLFLPFFSNV | TRDLFLPFFSNV | 107 | 6293 |
| HLA-A*30:01 | STQDLFLPF | STRDLFLPF | 2886 | 58 |
| HLA-A*68:01 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 22789 | 463 |
| HLA-A*03:02 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 11050 | 243 |
| HLA-A*11:01 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 11835 | 266 |
| HLA-A*74:01 | RSSVLHSTQ | RSSVLHSTR | 18484 | 447 |
| HLA-A*30:01 | STQDLFLPFF | STRDLFLPFF | 3497 | 105 |
| HLA-A*03:01 | KVFRSSVLHSTQ | KVFRSSVLHSTR | 9334 | 284 |
| HLA-A*11:01 | RSSVLHSTQ | RSSVLHSTR | 9744 | 302 |
| HLA-A*30:01 | STQDLFLPFFS | STRDLFLPFFS | 6813 | 321 |
| HLA-B*15:03 | TQDLFLPF | TRDLFLPF | 47 | 920 |
| HLA-A*30:01 | HSTQDLFLPF | HSTRDLFLPF | 7213 | 402 |
| HLA-A*30:01 | STQDLFLPFFSNV | STRDLFLPFFSNV | 4042 | 240 |
| HLA-B*27:05 | FRSSVLHSTQ | FRSSVLHSTR | 1533 | 115 |
| HLA-B*15:03 | TQDLFLPFF | TRDLFLPFF | 185 | 1800 |
| HLA-B*42:01 | STQDLFLPF | STRDLFLPF | 2706 | 447 |
| HLA-A*30:01 | RSSVLHSTQ | RSSVLHSTR | 677 | 129 |
| HLA-A*23:01 | STQDLFLPF | STRDLFLPF | 258 | 1243 |
| HLA-B*57:01 | STQDLFLPF | STRDLFLPF | 1089 | 319 |
| HLA-A*29:02 | STQDLFLPF | STRDLFLPF | 168 | 564 |
| HLA-C*12:03 | STQDLFLPF | STRDLFLPF | 279 | 85 |
| HLA-B*57:01 | STQDLFLPFF | STRDLFLPFF | 647 | 203 |
| HLA-C*12:02 | STQDLFLPF | STRDLFLPF | 316 | 113 |
| HLA-B*38:01 | LHSTQDLFL | LHSTRDLFL | 99 | 270 |
| HLA-A*26:01 | STQDLFLPF | STRDLFLPF | 627 | 249 |
| HLA-B*57:01 | HSTQDLFLPF | HSTRDLFLPF | 164 | 69 |
| HLA-B*57:01 | HSTQDLFLPFF | HSTRDLFLPFF | 394 | 169 |
| HLA-B*15:01 | STQDLFLPF | STRDLFLPF | 48 | 21 |
| HLA-B*15:01 | STQDLFLPFF | STRDLFLPFF | 230 | 104 |
| HLA-C*02:02 | STQDLFLPF | STRDLFLPF | 443 | 212 |
| HLA-C*02:10 | STQDLFLPF | STRDLFLPF | 443 | 212 |
| HLA-A*30:02 | STQDLFLPF | STRDLFLPF | 923 | 459 |
A67V
Reference VFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYF
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Mutated VFRSSVLHSTRDLFLPFFSNVTWFHVI‑‑SGTNGTKRFDNPVLPFNDGVYF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | VTWFHAIHV | VTWFHVI | 18 | - |
| HLA-A*02:06 | VTWFHAIHV | VTWFHVI | 40 | - |
| HLA-A*68:02 | VTWFHAIHV | VTWFHVI | 45 | - |
| HLA-C*15:02 | VTWFHAIHV | VTWFHVI | 107 | - |
| HLA-A*02:01 | VTWFHAIHV | VTWFHVI | 134 | - |
| HLA-C*12:03 | VTWFHAIHV | VTWFHVI | 341 | - |
| HLA-A*02:02 | VTWFHAIHV | VTWFHVI | 369 | - |
| HLA-C*16:01 | VTWFHAIHV | VTWFHVI | 421 | - |
| HLA-A*32:01 | VTWFHAIHV | VTWFHVI | 442 | - |
| HLA-A*68:02 | NVTWFHAIHV | NVTWFHVI | 16 | 5547 |
| HLA-A*02:11 | NVTWFHAIHV | NVTWFHVI | 125 | 10192 |
| HLA-A*02:06 | NVTWFHAIHV | NVTWFHVI | 263 | 10869 |
| HLA-C*15:02 | FSNVTWFHAIH | FSNVTWFHVI | 15930 | 389 |
| HLA-A*68:02 | FSNVTWFHAIH | FSNVTWFHVI | 13953 | 418 |
| HLA-A*02:02 | NVTWFHAIHV | NVTWFHVI | 468 | 14619 |
| HLA-B*58:01 | FSNVTWFHAIH | FSNVTWFHVI | 7866 | 292 |
| HLA-B*51:01 | LPFFSNVTWFHAIH | LPFFSNVTWFHVI | 11466 | 430 |
| HLA-B*58:01 | FSNVTWFHAIHV | FSNVTWFHVI | 6877 | 292 |
| HLA-A*11:01 | HAIHVSGTNGTK | HVISGTNGTK | 2998 | 135 |
| HLA-C*12:03 | FSNVTWFHAIH | FSNVTWFHVI | 8069 | 365 |
| HLA-A*02:06 | FSNVTWFHAIH | FSNVTWFHVI | 7820 | 387 |
| HLA-C*15:02 | FSNVTWFHA | FSNVTWFHV | 1444 | 73 |
| HLA-A*68:01 | AIHVSGTNGTKR | VISGTNGTKR | 5916 | 313 |
| HLA-A*68:02 | HAIHVSGTNGT | HVISGTNGT | 7705 | 415 |
| HLA-C*16:01 | FSNVTWFHAIH | FSNVTWFHVI | 5156 | 295 |
| HLA-B*42:01 | LPFFSNVTWFHAIH | LPFFSNVTWFHVI | 7318 | 437 |
| HLA-A*03:01 | HAIHVSGTNGTK | HVISGTNGTK | 3368 | 205 |
| HLA-A*02:11 | FSNVTWFHA | FSNVTWFHV | 366 | 23 |
| HLA-C*03:02 | FSNVTWFHAIHV | FSNVTWFHVI | 7468 | 494 |
| HLA-C*16:01 | FSNVTWFHAIHV | FSNVTWFHVI | 4412 | 295 |
| HLA-C*12:03 | FSNVTWFHAIHV | FSNVTWFHVI | 4736 | 365 |
| HLA-A*03:02 | HAIHVSGTNGTK | HVISGTNGTK | 4597 | 368 |
| HLA-C*03:02 | FSNVTWFHAIH | FSNVTWFHVI | 4526 | 494 |
| HLA-A*68:02 | FSNVTWFHA | FSNVTWFHV | 189 | 22 |
| HLA-A*02:01 | FSNVTWFHA | FSNVTWFHV | 1863 | 240 |
| HLA-A*68:02 | FFSNVTWFHAIHV | FFSNVTWFHVI | 492 | 3778 |
| HLA-A*02:06 | FSNVTWFHA | FSNVTWFHV | 182 | 24 |
| HLA-C*12:03 | FSNVTWFHA | FSNVTWFHV | 1369 | 181 |
| HLA-A*02:11 | FFSNVTWFHA | FFSNVTWFHV | 362 | 50 |
| HLA-C*15:02 | FSNVTWFHAIHV | FSNVTWFHVI | 2711 | 389 |
| HLA-A*02:02 | FSNVTWFHA | FSNVTWFHV | 741 | 115 |
| HLA-A*02:11 | FLPFFSNVTWFHA | FLPFFSNVTWFHV | 93 | 15 |
| HLA-C*16:01 | FSNVTWFHA | FSNVTWFHV | 906 | 156 |
| HLA-A*68:01 | HAIHVSGTNGTK | HVISGTNGTK | 174 | 30 |
| HLA-A*68:02 | FFSNVTWFHA | FFSNVTWFHV | 639 | 128 |
| HLA-A*02:11 | FFSNVTWFHAIHV | FFSNVTWFHVI | 219 | 1076 |
| HLA-A*02:01 | FFSNVTWFHA | FFSNVTWFHV | 1440 | 306 |
| HLA-A*02:01 | FLPFFSNVTWFHA | FLPFFSNVTWFHV | 424 | 94 |
| HLA-A*02:06 | FFSNVTWFHA | FFSNVTWFHV | 972 | 227 |
| HLA-A*02:02 | FFSNVTWFHA | FFSNVTWFHV | 328 | 79 |
| HLA-A*02:11 | FSNVTWFHAIHV | FSNVTWFHVI | 250 | 973 |
| HLA-A*68:02 | SNVTWFHAI | SNVTWFHVI | 196 | 729 |
| HLA-A*02:02 | FLPFFSNVTWFHA | FLPFFSNVTWFHV | 117 | 32 |
| HLA-A*03:02 | AIHVSGTNGTK | VISGTNGTK | 1179 | 326 |
| HLA-A*02:06 | SNVTWFHAI | SNVTWFHVI | 317 | 1145 |
| HLA-A*02:06 | FLPFFSNVTWFHA | FLPFFSNVTWFHV | 608 | 172 |
| HLA-C*03:02 | FSNVTWFHA | FSNVTWFHV | 1383 | 404 |
| HLA-A*68:02 | FSNVTWFHAI | FSNVTWFHVI | 124 | 418 |
| HLA-A*02:11 | FLPFFSNVTWFHAI | FLPFFSNVTWFHVI | 54 | 176 |
| HLA-A*02:06 | FLPFFSNVTWFHAI | FLPFFSNVTWFHVI | 457 | 1475 |
| HLA-A*02:06 | FSNVTWFHAI | FSNVTWFHVI | 123 | 387 |
| HLA-B*58:01 | FSNVTWFHA | FSNVTWFHV | 1061 | 354 |
| HLA-A*02:01 | FLPFFSNVTWFHAI | FLPFFSNVTWFHVI | 484 | 1319 |
| HLA-A*02:02 | FLPFFSNVTWFHAI | FLPFFSNVTWFHVI | 151 | 392 |
| HLA-A*02:11 | FSNVTWFHAI | FSNVTWFHVI | 398 | 973 |
| HLA-B*42:01 | LPFFSNVTWFHAI | LPFFSNVTWFHVI | 199 | 437 |
| HLA-A*11:01 | AIHVSGTNGTK | VISGTNGTK | 742 | 341 |
| HLA-A*03:01 | AIHVSGTNGTK | VISGTNGTK | 534 | 251 |
HV 69-70 deletion
Reference RSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAST
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Mutated RSSVLHSTRDLFLPFFSNVTWFHVI‑‑SGTNGTKRFDNPVLPFNDGVYFAST
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | VTWFHAIHV | VTWFHVI | 18 | - |
| HLA-A*02:06 | VTWFHAIHV | VTWFHVI | 40 | - |
| HLA-A*68:01 | HVSGTNGTKR | - | 42 | - |
| HLA-A*68:02 | VTWFHAIHV | VTWFHVI | 45 | - |
| HLA-A*68:01 | HVSGTNGTK | SGTNGTK | 54 | - |
| HLA-C*15:02 | VTWFHAIHV | VTWFHVI | 107 | - |
| HLA-A*02:01 | VTWFHAIHV | VTWFHVI | 134 | - |
| HLA-C*12:03 | VTWFHAIHV | VTWFHVI | 341 | - |
| HLA-A*02:02 | VTWFHAIHV | VTWFHVI | 369 | - |
| HLA-C*16:01 | VTWFHAIHV | VTWFHVI | 421 | - |
| HLA-A*32:01 | VTWFHAIHV | VTWFHVI | 442 | - |
| HLA-A*68:02 | NVTWFHAIHV | NVTWFHVI | 16 | 5547 |
| HLA-A*02:11 | NVTWFHAIHV | NVTWFHVI | 125 | 10192 |
| HLA-A*02:06 | NVTWFHAIHV | NVTWFHVI | 263 | 10869 |
| HLA-C*15:02 | FSNVTWFHAIH | FSNVTWFHVI | 15930 | 389 |
| HLA-A*68:02 | FSNVTWFHAIH | FSNVTWFHVI | 13953 | 418 |
| HLA-A*02:02 | NVTWFHAIHV | NVTWFHVI | 468 | 14619 |
| HLA-B*58:01 | FSNVTWFHAIH | FSNVTWFHVI | 7866 | 292 |
| HLA-B*51:01 | LPFFSNVTWFHAIH | LPFFSNVTWFHVI | 11466 | 430 |
| HLA-B*58:01 | FSNVTWFHAIHV | FSNVTWFHVI | 6877 | 292 |
| HLA-A*11:01 | HAIHVSGTNGTK | HVISGTNGTK | 2998 | 135 |
| HLA-C*12:03 | FSNVTWFHAIH | FSNVTWFHVI | 8069 | 365 |
| HLA-A*02:06 | FSNVTWFHAIH | FSNVTWFHVI | 7820 | 387 |
| HLA-A*68:01 | AIHVSGTNGTKR | VISGTNGTKR | 5916 | 313 |
| HLA-A*68:02 | HAIHVSGTNGT | HVISGTNGT | 7705 | 415 |
| HLA-C*16:01 | FSNVTWFHAIH | FSNVTWFHVI | 5156 | 295 |
| HLA-B*42:01 | LPFFSNVTWFHAIH | LPFFSNVTWFHVI | 7318 | 437 |
| HLA-A*03:01 | HAIHVSGTNGTK | HVISGTNGTK | 3368 | 205 |
| HLA-C*03:02 | FSNVTWFHAIHV | FSNVTWFHVI | 7468 | 494 |
| HLA-C*16:01 | FSNVTWFHAIHV | FSNVTWFHVI | 4412 | 295 |
| HLA-C*12:03 | FSNVTWFHAIHV | FSNVTWFHVI | 4736 | 365 |
| HLA-A*03:02 | HAIHVSGTNGTK | HVISGTNGTK | 4597 | 368 |
| HLA-C*03:02 | FSNVTWFHAIH | FSNVTWFHVI | 4526 | 494 |
| HLA-A*68:02 | FFSNVTWFHAIHV | FFSNVTWFHVI | 492 | 3778 |
| HLA-C*15:02 | FSNVTWFHAIHV | FSNVTWFHVI | 2711 | 389 |
| HLA-A*68:01 | HAIHVSGTNGTK | HVISGTNGTK | 174 | 30 |
| HLA-A*02:11 | FFSNVTWFHAIHV | FFSNVTWFHVI | 219 | 1076 |
| HLA-A*02:11 | FSNVTWFHAIHV | FSNVTWFHVI | 250 | 973 |
| HLA-A*03:02 | AIHVSGTNGTK | VISGTNGTK | 1179 | 326 |
| HLA-A*11:01 | AIHVSGTNGTK | VISGTNGTK | 742 | 341 |
| HLA-A*03:01 | AIHVSGTNGTK | VISGTNGTK | 534 | 251 |
Y144 deletion
Reference IVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFE
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Mutated IVNNATNVVIKVCEFQFCNDPFLGV‑YHKNNKSWMESEFRVYSSANNCTFE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | YYHKNNKSWM | - | 19 | - |
| HLA-B*15:03 | FQFCNDPFLGVYY | - | 47 | - |
| HLA-C*14:02 | YYHKNNKSW | - | 52 | - |
| HLA-A*29:02 | QFCNDPFLGVYY | - | 113 | - |
| HLA-A*24:02 | YYHKNNKSW | - | 116 | - |
| HLA-B*15:01 | FQFCNDPFLGVYY | - | 129 | - |
| HLA-A*23:01 | YYHKNNKSW | - | 150 | - |
| HLA-A*29:02 | FQFCNDPFLGVYY | - | 184 | - |
| HLA-A*01:01 | CNDPFLGVYY | - | 202 | - |
| HLA-C*14:02 | YYHKNNKSWMESEF | - | 227 | - |
| HLA-A*29:02 | CNDPFLGVYY | - | 439 | - |
| HLA-A*11:01 | GVYYHKNNK | GVYHKNNK | 51 | 5741 |
| HLA-A*03:01 | GVYYHKNNK | GVYHKNNK | 27 | 2231 |
| HLA-A*30:01 | GVYYHKNNK | GVYHKNNK | 51 | 3606 |
| HLA-A*03:01 | LGVYYHKNNK | LGVYHKNNK | 131 | 8411 |
| HLA-A*03:02 | GVYYHKNNK | GVYHKNNK | 119 | 6497 |
| HLA-A*68:01 | GVYYHKNNK | GVYHKNNK | 363 | 17786 |
| HLA-A*31:01 | GVYYHKNNK | GVYHKNNK | 375 | 9671 |
| HLA-A*33:03 | DPFLGVYYHK | DPFLGVYHK | 280 | 4172 |
| HLA-A*68:01 | LGVYYHKNNK | LGVYHKNNK | 494 | 3973 |
| HLA-C*14:02 | VYYHKNNKSW | VYHKNNKSW | 1115 | 238 |
| HLA-B*15:03 | FQFCNDPFLGVYYH | FQFCNDPFLGVYH | 1280 | 298 |
| HLA-C*14:02 | VYYHKNNKSWM | VYHKNNKSWM | 327 | 108 |
| HLA-A*68:01 | DPFLGVYYHK | DPFLGVYHK | 398 | 969 |
| HLA-A*03:01 | FLGVYYHKNNK | FLGVYHKNNK | 455 | 1078 |
E484K
Reference SNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYR
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Mutated SNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTNGVGYQPYR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*40:02 | VEGFNCYFPL | VKGFNCYFPL | 31 | 7011 |
| HLA-B*40:01 | VEGFNCYFPL | VKGFNCYFPL | 76 | 15210 |
| HLA-A*11:01 | GSTPCNGVE | GSTPCNGVK | 32767 | 444 |
| HLA-A*68:02 | EGFNCYFPL | KGFNCYFPL | 117 | 3877 |
| HLA-A*30:01 | EGFNCYFPL | KGFNCYFPL | 5413 | 164 |
| HLA-A*32:01 | EGFNCYFPL | KGFNCYFPL | 6548 | 210 |
| HLA-A*30:02 | EGFNCYFPLQSY | KGFNCYFPLQSY | 1806 | 72 |
| HLA-B*15:01 | GVEGFNCYF | GVKGFNCYF | 3125 | 132 |
| HLA-A*31:01 | EGFNCYFPL | KGFNCYFPL | 4227 | 226 |
| HLA-B*15:03 | EGFNCYFPL | KGFNCYFPL | 2980 | 212 |
| HLA-A*02:11 | EGFNCYFPL | KGFNCYFPL | 2508 | 259 |
| HLA-B*15:03 | GVEGFNCYF | GVKGFNCYF | 1657 | 199 |
| HLA-A*02:06 | EGFNCYFPL | KGFNCYFPL | 1266 | 169 |
| HLA-B*35:01 | TPCNGVEGF | TPCNGVKGF | 319 | 1754 |
| HLA-B*15:03 | VEGFNCYFPLQSY | VKGFNCYFPLQSY | 1081 | 208 |
| HLA-B*35:01 | NGVEGFNCY | NGVKGFNCY | 346 | 1439 |
| HLA-C*16:01 | EGFNCYFPL | KGFNCYFPL | 1861 | 498 |
| HLA-B*15:03 | VEGFNCYFPL | VKGFNCYFPL | 943 | 280 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
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Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
Q677H
Reference GAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAE
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Mutated GAEHVNNSYECDIPIGAGICASYQTHTNSPRRARSVASQSIIAYTMSLGAE
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*31:01 | ASYQTQTNSPRR | ASYQTHTNSPRR | 433 | 121 |
| HLA-A*31:01 | ASYQTQTNSPR | ASYQTHTNSPR | 139 | 45 |
| HLA-A*30:01 | QTNSPRRAR | HTNSPRRAR | 1146 | 391 |
| HLA-B*07:02 | QTNSPRRARSVA | HTNSPRRARSVA | 249 | 100 |
| HLA-A*68:01 | QTQTNSPRR | QTHTNSPRR | 119 | 51 |
| HLA-A*30:01 | QTNSPRRARSVA | HTNSPRRARSVA | 662 | 288 |
| HLA-B*42:01 | QTNSPRRARSVA | HTNSPRRARSVA | 842 | 369 |
| HLA-A*68:01 | QTNSPRRAR | HTNSPRRAR | 95 | 43 |
| HLA-A*33:03 | QTNSPRRAR | HTNSPRRAR | 48 | 22 |
| HLA-A*68:01 | YQTQTNSPRR | YQTHTNSPRR | 206 | 99 |
| HLA-A*31:01 | YQTQTNSPR | YQTHTNSPR | 441 | 220 |
F888L
Reference PLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQ
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PLLTDEMIAQYTSALLAGTITSGWTLGAGAALQIPFAMQMAYRFNGIGVTQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | ALLAGTITSGWTF | ALLAGTITSGWTL | 3766 | 20 |
| HLA-A*02:11 | LLAGTITSGWTF | LLAGTITSGWTL | 2814 | 15 |
| HLA-A*02:02 | LLAGTITSGWTF | LLAGTITSGWTL | 2639 | 26 |
| HLA-A*02:01 | ALLAGTITSGWTF | ALLAGTITSGWTL | 6503 | 67 |
| HLA-A*02:01 | LLAGTITSGWTF | LLAGTITSGWTL | 5299 | 62 |
| HLA-C*14:02 | TFGAGAAL | TLGAGAAL | 117 | 9056 |
| HLA-A*02:02 | ALLAGTITSGWTF | ALLAGTITSGWTL | 7387 | 110 |
| HLA-A*02:11 | TFGAGAALQI | TLGAGAALQI | 11512 | 264 |
| HLA-A*02:02 | TFGAGAALQI | TLGAGAALQI | 7571 | 192 |
| HLA-B*15:01 | LLAGTITSGWTF | LLAGTITSGWTL | 86 | 3256 |
| HLA-A*02:11 | GTITSGWTF | GTITSGWTL | 7105 | 250 |
| HLA-B*15:01 | GTITSGWTF | GTITSGWTL | 144 | 2911 |
| HLA-B*15:01 | AGTITSGWTF | AGTITSGWTL | 305 | 5944 |
| HLA-A*02:06 | LLAGTITSGWTF | LLAGTITSGWTL | 5077 | 270 |
| HLA-B*15:03 | GTITSGWTF | GTITSGWTL | 88 | 1485 |
| HLA-A*02:06 | ALLAGTITSGWTF | ALLAGTITSGWTL | 6727 | 447 |
| HLA-A*23:01 | GTITSGWTF | GTITSGWTL | 350 | 4926 |
| HLA-A*68:02 | TSGWTFGAGAAL | TSGWTLGAGAAL | 187 | 2442 |
| HLA-B*15:03 | AGTITSGWTF | AGTITSGWTL | 492 | 5538 |
| HLA-C*03:03 | FGAGAALQI | LGAGAALQI | 270 | 3033 |
| HLA-C*03:04 | FGAGAALQI | LGAGAALQI | 270 | 3033 |
| HLA-A*02:06 | GTITSGWTF | GTITSGWTL | 1481 | 149 |
| HLA-A*68:02 | GWTFGAGAAL | GWTLGAGAAL | 362 | 3498 |
| HLA-C*12:03 | FGAGAALQI | LGAGAALQI | 302 | 2807 |
| HLA-A*02:02 | LLAGTITSGWTFG | LLAGTITSGWTLG | 3915 | 444 |
| HLA-B*58:01 | GTITSGWTF | GTITSGWTL | 56 | 475 |
| HLA-B*58:01 | AGTITSGWTF | AGTITSGWTL | 140 | 1178 |
| HLA-C*12:03 | WTFGAGAAL | WTLGAGAAL | 31 | 256 |
| HLA-B*57:01 | AGTITSGWTF | AGTITSGWTL | 297 | 2383 |
| HLA-A*02:11 | ITSGWTFGA | ITSGWTLGA | 51 | 399 |
| HLA-A*68:02 | ITSGWTFGA | ITSGWTLGA | 32 | 247 |
| HLA-B*57:01 | GTITSGWTF | GTITSGWTL | 196 | 1443 |
| HLA-C*17:01 | GTITSGWTF | GTITSGWTL | 3213 | 442 |
| HLA-B*58:01 | LAGTITSGWTF | LAGTITSGWTL | 119 | 847 |
| HLA-B*58:01 | LLAGTITSGWTF | LLAGTITSGWTL | 220 | 1487 |
| HLA-B*57:01 | LLAGTITSGWTF | LLAGTITSGWTL | 393 | 2547 |
| HLA-A*02:06 | ITSGWTFGA | ITSGWTLGA | 82 | 527 |
| HLA-A*26:01 | WTFGAGAAL | WTLGAGAAL | 351 | 2179 |
| HLA-C*17:01 | FGAGAALQI | LGAGAALQI | 268 | 1642 |
| HLA-B*57:01 | LAGTITSGWTF | LAGTITSGWTL | 308 | 1848 |
| HLA-C*12:02 | WTFGAGAAL | WTLGAGAAL | 66 | 382 |
| HLA-A*02:02 | ITSGWTFGA | ITSGWTLGA | 257 | 1444 |
| HLA-A*68:02 | WTFGAGAAL | WTLGAGAAL | 13 | 73 |
| HLA-A*68:02 | WTFGAGAALQI | WTLGAGAALQI | 149 | 833 |
| HLA-A*68:02 | TITSGWTFGA | TITSGWTLGA | 56 | 312 |
| HLA-A*02:01 | ITSGWTFGA | ITSGWTLGA | 464 | 2512 |
| HLA-B*15:01 | WTFGAGAALQIPF | WTLGAGAALQIPF | 308 | 1443 |
| HLA-C*16:01 | FGAGAALQI | LGAGAALQI | 383 | 1788 |
| HLA-B*15:03 | WTFGAGAALQIPF | WTLGAGAALQIPF | 333 | 1473 |
| HLA-B*15:03 | WTFGAGAAL | WTLGAGAAL | 30 | 131 |
| HLA-C*02:02 | WTFGAGAAL | WTLGAGAAL | 308 | 1256 |
| HLA-C*02:10 | WTFGAGAAL | WTLGAGAAL | 308 | 1256 |
| HLA-B*35:01 | WTFGAGAAL | WTLGAGAAL | 240 | 955 |
| HLA-B*15:01 | WTFGAGAAL | WTLGAGAAL | 210 | 828 |
| HLA-A*32:01 | GTITSGWTF | GTITSGWTL | 115 | 411 |
| HLA-C*03:02 | GTITSGWTF | GTITSGWTL | 321 | 1104 |
| HLA-A*02:11 | LLAGTITSGWTFGA | LLAGTITSGWTLGA | 197 | 641 |
| HLA-A*02:02 | WTFGAGAAL | WTLGAGAAL | 146 | 473 |
| HLA-C*15:02 | FGAGAALQI | LGAGAALQI | 328 | 949 |
| HLA-C*14:02 | WTFGAGAAL | WTLGAGAAL | 106 | 296 |
| HLA-C*16:01 | WTFGAGAAL | WTLGAGAAL | 41 | 111 |
| HLA-C*03:02 | WTFGAGAAL | WTLGAGAAL | 10 | 25 |
| HLA-A*02:11 | WTFGAGAAL | WTLGAGAAL | 132 | 308 |
| HLA-C*17:01 | WTFGAGAAL | WTLGAGAAL | 108 | 247 |
N protein
S2 deletion
Reference MSDNGPQNQRNAPRITFGGPSDSTGSN
| |||||||||||||||||||||||||
Mutated M‑YNGPQNQRNGPRITFGGPSDSTGSN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | MSDNGPQNQR | MYNGPQNQR | 37 | 480 |
| HLA-A*33:03 | MSDNGPQNQR | MYNGPQNQR | 290 | 39 |
| HLA-A*33:03 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 565 | 140 |
| HLA-A*31:01 | MSDNGPQNQR | MYNGPQNQR | 217 | 64 |
| HLA-A*31:01 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 627 | 303 |
D3Y
Reference MSDNGPQNQRNAPRITFGGPSDSTGSNQ
|| |||||||||||||||||||||||||
Mutated M‑YNGPQNQRNGPRITFGGPSDSTGSNQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:01 | MSDNGPQNQR | MYNGPQNQR | 37 | 480 |
| HLA-A*33:03 | MSDNGPQNQR | MYNGPQNQR | 290 | 39 |
| HLA-A*33:03 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 565 | 140 |
| HLA-A*31:01 | MSDNGPQNQR | MYNGPQNQR | 217 | 64 |
| HLA-A*31:01 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 627 | 303 |
A12G
Reference MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARS
||||||||||| |||||||||||||||||||||||||
Mutated M‑YNGPQNQRNGPRITFGGPSDSTGSNQNGERSGARS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*07:02 | GPQNQRNAPRI | GPQNQRNGPRI | 496 | 3951 |
| HLA-A*33:03 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 565 | 140 |
| HLA-B*07:02 | APRITFGGPS | GPRITFGGPS | 191 | 673 |
| HLA-A*31:01 | MSDNGPQNQRNAPR | MYNGPQNQRNGPR | 627 | 303 |
T205I
Reference SQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated SQASSRSSSRSRNSSRNSTPGSSRGISPARMAGNGGDAALALLLLDRLNQL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*01:02 | STPGSSRGT | STPGSSRGI | 10810 | 468 |
| HLA-A*30:01 | GTSPARMAG | GISPARMAG | 493 | 1901 |
| HLA-A*68:01 | SSRGTSPAR | SSRGISPAR | 186 | 61 |
| HLA-A*68:01 | STPGSSRGTSPAR | STPGSSRGISPAR | 860 | 288 |
| HLA-A*30:01 | RGTSPARMA | RGISPARMA | 1160 | 422 |
| HLA-A*68:01 | GSSRGTSPAR | GSSRGISPAR | 615 | 248 |
| HLA-A*31:01 | GSSRGTSPAR | GSSRGISPAR | 262 | 111 |
| HLA-A*33:03 | SSRGTSPAR | SSRGISPAR | 106 | 46 |
| HLA-A*33:03 | STPGSSRGTSPAR | STPGSSRGISPAR | 968 | 473 |
M protein
I82T
Reference LWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTR
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LWPVTLACFVLAAVYRINWITGGIATAMACLVGLMWLSYFIASFRLFARTR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*14:02 | NWITGGIAI | NWITGGIAT | 109 | 4410 |
| HLA-A*68:02 | IAMACLVGL | TAMACLVGL | 424 | 40 |
| HLA-B*27:05 | YRINWITGGIAI | YRINWITGGIAT | 85 | 885 |
| HLA-A*68:02 | AIAMACLVGL | ATAMACLVGL | 1414 | 203 |
| HLA-A*02:11 | WITGGIAIA | WITGGIATA | 788 | 138 |
| HLA-A*68:02 | IAMACLVGLM | TAMACLVGLM | 2691 | 495 |
| HLA-A*02:06 | WITGGIAIA | WITGGIATA | 477 | 117 |
| HLA-A*02:01 | AIAMACLVGL | ATAMACLVGL | 97 | 388 |
| HLA-A*02:06 | IAIAMACLV | IATAMACLV | 90 | 359 |
| HLA-A*02:11 | AIAMACLVGL | ATAMACLVGL | 53 | 208 |
| HLA-A*02:02 | WITGGIAIA | WITGGIATA | 333 | 88 |
| HLA-A*02:11 | IAIAMACLV | IATAMACLV | 140 | 524 |
| HLA-A*02:02 | AIAMACLVGL | ATAMACLVGL | 41 | 133 |
| HLA-B*58:01 | IAMACLVGL | TAMACLVGL | 423 | 1182 |
| HLA-A*02:11 | GIAIAMACL | GIATAMACL | 350 | 144 |
| HLA-A*68:02 | WITGGIAIA | WITGGIATA | 918 | 378 |
| HLA-B*58:01 | IAMACLVGLM | TAMACLVGLM | 479 | 1090 |
| HLA-C*12:03 | IAIAMACLV | IATAMACLV | 47 | 105 |
| HLA-A*02:02 | GIAIAMACL | GIATAMACL | 110 | 50 |
| HLA-B*57:01 | IAMACLVGLMW | TAMACLVGLMW | 65 | 141 |
| HLA-A*02:02 | GIAIAMACLV | GIATAMACLV | 84 | 39 |
| HLA-C*12:02 | IAIAMACLV | IATAMACLV | 336 | 715 |
| HLA-A*02:06 | GIAIAMACL | GIATAMACL | 662 | 317 |
| HLA-A*02:02 | GGIAIAMACL | GGIATAMACL | 596 | 288 |
| HLA-B*58:01 | IAMACLVGLMW | TAMACLVGLMW | 19 | 39 |
| HLA-A*68:02 | IAIAMACLV | IATAMACLV | 168 | 339 |
| HLA-A*02:01 | IAMACLVGL | TAMACLVGL | 122 | 246 |
| HLA-A*02:11 | IAMACLVGL | TAMACLVGL | 70 | 141 |
| HLA-B*57:01 | AIAMACLVGLMW | ATAMACLVGLMW | 538 | 268 |
E protein
L21F
Reference MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNI
|||||||||||||||||||| |||||||||||||||||||||||||
Mutated MYSFVSEETGTLIVNSVLLFFAFVVFLLVTLAILTALRLCAYCCNI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | FLAFVVFL | FFAFVVFL | 21 | 5433 |
| HLA-A*02:11 | IVNSVLLFL | IVNSVLLFF | 16 | 3454 |
| HLA-A*02:02 | FLAFVVFL | FFAFVVFL | 24 | 3923 |
| HLA-A*02:01 | FLAFVVFLL | FFAFVVFLL | 5 | 777 |
| HLA-A*02:02 | IVNSVLLFL | IVNSVLLFF | 25 | 3332 |
| HLA-A*02:11 | FLAFVVFLLVTL | FFAFVVFLLVTL | 28 | 3402 |
| HLA-A*02:01 | FLAFVVFL | FFAFVVFL | 107 | 11823 |
| HLA-A*02:11 | FLAFVVFLLVTLAI | FFAFVVFLLVTLAI | 120 | 11658 |
| HLA-A*02:11 | TLIVNSVLLFL | TLIVNSVLLFF | 83 | 7335 |
| HLA-A*02:01 | IVNSVLLFL | IVNSVLLFF | 74 | 6206 |
| HLA-A*02:02 | LIVNSVLLFL | LIVNSVLLFF | 70 | 5517 |
| HLA-A*02:02 | LFLAFVVFLL | LFFAFVVFLL | 16 | 1201 |
| HLA-A*02:02 | FLAFVVFLLVTL | FFAFVVFLLVTL | 36 | 2659 |
| HLA-A*02:11 | LFLAFVVFLL | LFFAFVVFLL | 18 | 1305 |
| HLA-A*02:11 | FLAFVVFLLVT | FFAFVVFLLVT | 186 | 13267 |
| HLA-A*02:11 | FLAFVVFLLV | FFAFVVFLLV | 6 | 398 |
| HLA-A*02:02 | FLAFVVFLLV | FFAFVVFLLV | 12 | 792 |
| HLA-A*02:11 | LIVNSVLLFL | LIVNSVLLFF | 116 | 7571 |
| HLA-A*02:02 | FLAFVVFLLVTLAI | FFAFVVFLLVTLAI | 185 | 10954 |
| HLA-A*02:01 | FLAFVVFLLV | FFAFVVFLLV | 33 | 1870 |
| HLA-A*02:06 | FLAFVVFLL | FFAFVVFLL | 20 | 1108 |
| HLA-A*02:11 | FLAFVVFLL | FFAFVVFLL | 2 | 102 |
| HLA-A*02:02 | TLIVNSVLLFL | TLIVNSVLLFF | 147 | 7129 |
| HLA-A*02:06 | IVNSVLLFL | IVNSVLLFF | 43 | 1905 |
| HLA-A*02:01 | LFLAFVVFLL | LFFAFVVFLL | 87 | 3662 |
| HLA-A*02:01 | FLAFVVFLLVTL | FFAFVVFLLVTL | 180 | 7525 |
| HLA-A*02:02 | FLAFVVFLL | FFAFVVFLL | 3 | 125 |
| HLA-A*02:02 | FLAFVVFLLVT | FFAFVVFLLVT | 334 | 13428 |
| HLA-A*02:11 | FLAFVVFLLVTLA | FFAFVVFLLVTLA | 128 | 4980 |
| HLA-A*02:02 | FLAFVVFLLVTLA | FFAFVVFLLVTLA | 101 | 3916 |
| HLA-A*02:01 | TLIVNSVLLFL | TLIVNSVLLFF | 255 | 9664 |
| HLA-A*02:01 | LIVNSVLLFL | LIVNSVLLFF | 229 | 8576 |
| HLA-A*02:06 | LIVNSVLLFL | LIVNSVLLFF | 147 | 4187 |
| HLA-A*68:02 | IVNSVLLFL | IVNSVLLFF | 379 | 10374 |
| HLA-A*29:02 | IVNSVLLFL | IVNSVLLFF | 2008 | 77 |
| HLA-A*02:11 | LFLAFVVFLLV | LFFAFVVFLLV | 165 | 3596 |
| HLA-C*14:02 | FLAFVVFL | FFAFVVFL | 10352 | 482 |
| HLA-C*14:02 | FLAFVVFLL | FFAFVVFLL | 4203 | 199 |
| HLA-B*15:01 | IVNSVLLFL | IVNSVLLFF | 2718 | 133 |
| HLA-C*15:02 | IVNSVLLFL | IVNSVLLFF | 126 | 2497 |
| HLA-B*15:01 | LIVNSVLLFL | LIVNSVLLFF | 1903 | 97 |
| HLA-A*02:02 | LFLAFVVFLLV | LFFAFVVFLLV | 427 | 7784 |
| HLA-A*02:01 | FLAFVVFLLVTLA | FFAFVVFLLVTLA | 485 | 8803 |
| HLA-C*17:01 | IVNSVLLFL | IVNSVLLFF | 140 | 2532 |
| HLA-A*02:02 | LFLAFVVFLLVTL | LFFAFVVFLLVTL | 399 | 6794 |
| HLA-B*15:03 | IVNSVLLFL | IVNSVLLFF | 1562 | 100 |
| HLA-A*23:01 | IVNSVLLFL | IVNSVLLFF | 4286 | 322 |
| HLA-A*23:01 | FLAFVVFLL | FFAFVVFLL | 5681 | 432 |
| HLA-C*17:01 | FLAFVVFLL | FFAFVVFLL | 436 | 4229 |
| HLA-B*15:03 | LIVNSVLLFL | LIVNSVLLFF | 4086 | 471 |
| HLA-B*58:01 | IVNSVLLFL | IVNSVLLFF | 712 | 96 |
| HLA-C*03:03 | LAFVVFLLV | FAFVVFLLV | 1855 | 254 |
| HLA-C*03:04 | LAFVVFLLV | FAFVVFLLV | 1855 | 254 |
| HLA-A*02:11 | SVLLFLAFVVFLL | SVLLFFAFVVFLL | 141 | 1000 |
| HLA-A*02:02 | SVLLFLAFVVFLL | SVLLFFAFVVFLL | 157 | 1091 |
| HLA-A*02:11 | LAFVVFLLV | FAFVVFLLV | 349 | 56 |
| HLA-C*12:03 | LAFVVFLLV | FAFVVFLLV | 190 | 31 |
| HLA-C*12:02 | LAFVVFLLV | FAFVVFLLV | 1465 | 256 |
| HLA-C*03:02 | LAFVVFLLV | FAFVVFLLV | 1034 | 184 |
| HLA-C*12:03 | LAFVVFLL | FAFVVFLL | 2342 | 431 |
| HLA-A*68:02 | FLAFVVFLL | FFAFVVFLL | 169 | 801 |
| HLA-A*02:06 | LAFVVFLLV | FAFVVFLLV | 308 | 67 |
| HLA-A*68:02 | LAFVVFLLV | FAFVVFLLV | 390 | 89 |
| HLA-A*02:01 | LAFVVFLLV | FAFVVFLLV | 1554 | 358 |
| HLA-C*16:01 | LAFVVFLLV | FAFVVFLLV | 411 | 101 |
| HLA-C*16:01 | LAFVVFLL | FAFVVFLL | 1366 | 350 |
| HLA-A*02:06 | FLAFVVFLLV | FFAFVVFLLV | 169 | 651 |
| HLA-A*02:02 | LAFVVFLLV | FAFVVFLLV | 1446 | 408 |
| HLA-A*29:02 | FLAFVVFLL | FFAFVVFLL | 1280 | 366 |
| HLA-A*32:01 | IVNSVLLFL | IVNSVLLFF | 574 | 176 |
| HLA-A*32:01 | LLFLAFVVF | LLFFAFVVF | 1029 | 347 |
| HLA-C*03:02 | NSVLLFLAF | NSVLLFFAF | 93 | 270 |
| HLA-B*15:03 | NSVLLFLAF | NSVLLFFAF | 51 | 144 |
| HLA-C*03:02 | IVNSVLLFL | IVNSVLLFF | 483 | 182 |
| HLA-B*15:03 | VNSVLLFLAF | VNSVLLFFAF | 355 | 916 |
| HLA-C*14:02 | LFLAFVVFL | LFFAFVVFL | 452 | 179 |
| HLA-B*15:01 | NSVLLFLAF | NSVLLFFAF | 445 | 1122 |
| HLA-A*02:01 | NSVLLFLAFV | NSVLLFFAFV | 218 | 504 |
| HLA-C*15:02 | LAFVVFLLV | FAFVVFLLV | 725 | 323 |
| HLA-C*12:03 | IVNSVLLFL | IVNSVLLFF | 327 | 727 |
| HLA-A*02:02 | VLLFLAFVVFL | VLLFFAFVVFL | 934 | 457 |
| HLA-A*02:01 | SVLLFLAFV | SVLLFFAFV | 25 | 51 |
| HLA-C*16:01 | NSVLLFLAF | NSVLLFFAF | 264 | 537 |
NS6 protein
F2 deletion
Reference MFHLVDFQVTIAEILLIIMRTFKVSIW
| |||||||||||||||||||||||||
Mutated M‑HLVDFQVTIAEILLIIMRTFKVSIW
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | FHLVDFQVTI | - | 5 | - |
| HLA-A*02:02 | FHLVDFQVTI | - | 9 | - |
| HLA-A*02:01 | FHLVDFQVTI | - | 13 | - |
| HLA-A*02:06 | FHLVDFQVTI | - | 46 | - |
| HLA-B*38:01 | FHLVDFQVTI | - | 152 | - |
| HLA-A*02:11 | FHLVDFQVTIA | - | 285 | - |
| HLA-A*02:02 | FHLVDFQVTIA | - | 411 | - |
| HLA-B*38:01 | MFHLVDFQVTI | MHLVDFQVTI | 6291 | 62 |
| HLA-A*68:02 | MFHLVDFQV | MHLVDFQV | 350 | 17340 |
| HLA-A*02:11 | MFHLVDFQV | MHLVDFQV | 281 | 3270 |
| HLA-A*68:02 | MFHLVDFQVTI | MHLVDFQVTI | 2857 | 329 |
| HLA-A*02:02 | MFHLVDFQV | MHLVDFQV | 403 | 3464 |
| HLA-A*02:02 | MFHLVDFQVTI | MHLVDFQVTI | 51 | 11 |
| HLA-A*02:06 | MFHLVDFQVTI | MHLVDFQVTI | 303 | 76 |
| HLA-A*02:01 | MFHLVDFQVTI | MHLVDFQVTI | 81 | 24 |
| HLA-A*02:11 | MFHLVDFQVTI | MHLVDFQVTI | 25 | 8 |
NS9b protein
H9D
Reference MDPKISEMHPALRLVDPQIQLAVTRMENAVGRDQ
|||||||| |||||||||||||||||||||||||
Mutated MDPKISEMDPALRLVDPQIQLAVTRMENAVGRDQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*42:01 | HPALRLVDPQIQL | DPALRLVDPQIQL | 148 | 6460 |
| HLA-B*42:01 | HPALRLVDPQI | DPALRLVDPQI | 297 | 7325 |
| HLA-C*16:01 | ISEMHPAL | ISEMDPAL | 248 | 3759 |
| HLA-A*30:01 | KISEMHPAL | KISEMDPAL | 455 | 5696 |
| HLA-A*31:01 | ISEMHPALR | ISEMDPALR | 196 | 2247 |
| HLA-B*42:01 | KISEMHPAL | KISEMDPAL | 213 | 2223 |
| HLA-B*07:02 | KISEMHPAL | KISEMDPAL | 436 | 3836 |
| HLA-C*16:01 | KISEMHPAL | KISEMDPAL | 189 | 1661 |
| HLA-A*32:01 | KISEMHPAL | KISEMDPAL | 124 | 1005 |
| HLA-A*33:03 | ISEMHPALR | ISEMDPALR | 395 | 2809 |
| HLA-A*02:11 | ISEMHPALRLV | ISEMDPALRLV | 778 | 140 |
| HLA-A*68:02 | EMHPALRLV | EMDPALRLV | 211 | 1079 |
| HLA-C*03:03 | KISEMHPAL | KISEMDPAL | 177 | 809 |
| HLA-C*03:04 | KISEMHPAL | KISEMDPAL | 177 | 809 |
| HLA-A*31:01 | KISEMHPALR | KISEMDPALR | 28 | 126 |
| HLA-A*02:11 | EMHPALRLV | EMDPALRLV | 249 | 60 |
| HLA-A*33:03 | KISEMHPALR | KISEMDPALR | 308 | 1254 |
| HLA-C*03:02 | KISEMHPAL | KISEMDPAL | 371 | 1480 |
| HLA-A*02:01 | SEMHPALRLV | SEMDPALRLV | 975 | 279 |
| HLA-A*02:11 | SEMHPALRLV | SEMDPALRLV | 122 | 39 |
| HLA-C*17:01 | KISEMHPAL | KISEMDPAL | 241 | 721 |
| HLA-A*02:11 | KISEMHPAL | KISEMDPAL | 14 | 37 |
| HLA-B*40:01 | KISEMHPALRL | KISEMDPALRL | 664 | 253 |
| HLA-B*15:03 | KISEMHPAL | KISEMDPAL | 436 | 1138 |
| HLA-A*74:01 | KISEMHPALR | KISEMDPALR | 419 | 1090 |
| HLA-A*02:06 | EMHPALRLV | EMDPALRLV | 1042 | 425 |
| HLA-A*03:01 | KISEMHPALR | KISEMDPALR | 414 | 1011 |
| HLA-B*40:01 | ISEMHPALRL | ISEMDPALRL | 404 | 179 |
NS9c protein
L52F
Reference VKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||
Mutated VKPLLVPHHVVATVQEIQLQAAVGEFLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | QLQAAVGEL | QLQAAVGEF | 120 | 5031 |
| HLA-A*02:11 | GELLLLEWLA | GEFLLLEWLA | 267 | 9767 |
| HLA-B*15:01 | QLQAAVGEL | QLQAAVGEF | 2048 | 65 |
| HLA-A*02:01 | GELLLLEWLA | GEFLLLEWLA | 409 | 12481 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVGEF | 92 | 2789 |
| HLA-B*15:03 | QLQAAVGEL | QLQAAVGEF | 2231 | 81 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVGEF | 170 | 4413 |
| HLA-A*02:11 | ELLLLEWLA | EFLLLEWLA | 345 | 8719 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVGEF | 338 | 7506 |
| HLA-B*15:01 | IQLQAAVGEL | IQLQAAVGEF | 467 | 24 |
| HLA-B*15:03 | IQLQAAVGEL | IQLQAAVGEF | 168 | 10 |
| HLA-B*15:03 | LQAAVGEL | LQAAVGEF | 295 | 18 |
| HLA-B*15:03 | VQEIQLQAAVGEL | VQEIQLQAAVGEF | 6575 | 430 |
| HLA-A*02:11 | LLLLEWLAM | FLLLEWLAM | 283 | 20 |
| HLA-B*40:01 | QEIQLQAAVGEL | QEIQLQAAVGEF | 201 | 2708 |
| HLA-B*15:01 | LQAAVGEL | LQAAVGEF | 1712 | 138 |
| HLA-A*02:01 | LLLLEWLAM | FLLLEWLAM | 573 | 54 |
| HLA-A*02:11 | LLLLEWLA | FLLLEWLA | 844 | 82 |
| HLA-A*02:02 | LLLLEWLAM | FLLLEWLAM | 1379 | 150 |
| HLA-B*44:03 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1386 | 164 |
| HLA-A*02:06 | LLLLEWLAM | FLLLEWLAM | 763 | 94 |
| HLA-A*02:01 | LLLLEWLA | FLLLEWLA | 1287 | 163 |
| HLA-B*44:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1403 | 179 |
| HLA-A*02:02 | LLLLEWLA | FLLLEWLA | 2866 | 389 |
| HLA-C*03:02 | LLLLEWLAM | FLLLEWLAM | 2566 | 358 |
| HLA-A*02:06 | IQLQAAVGEL | IQLQAAVGEF | 280 | 1893 |
| HLA-A*02:06 | LLLLEWLA | FLLLEWLA | 2803 | 489 |
| HLA-A*02:02 | LLLLEWLAMA | FLLLEWLAMA | 67 | 13 |
| HLA-A*02:01 | ELLLLEWLAMA | EFLLLEWLAMA | 1276 | 266 |
| HLA-A*02:11 | QLQAAVGELLL | QLQAAVGEFLL | 733 | 157 |
| HLA-B*40:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 334 | 1488 |
| HLA-A*02:06 | LLLLEWLAMA | FLLLEWLAMA | 49 | 11 |
| HLA-A*02:11 | LLLLEWLAMAVM | FLLLEWLAMAVM | 926 | 212 |
| HLA-A*02:02 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 714 | 164 |
| HLA-A*02:01 | LLLLEWLAMAVML | FLLLEWLAMAVML | 1353 | 320 |
| HLA-A*02:11 | LLLLEWLAMAVML | FLLLEWLAMAVML | 385 | 97 |
| HLA-A*02:11 | LQAAVGELLL | LQAAVGEFLL | 1331 | 346 |
| HLA-A*02:01 | LLLLEWLAMA | FLLLEWLAMA | 23 | 6 |
| HLA-A*02:11 | ELLLLEWLAMA | EFLLLEWLAMA | 299 | 79 |
| HLA-A*02:11 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 544 | 146 |
| HLA-A*02:01 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 1361 | 373 |
| HLA-B*40:01 | GELLLLEWLAM | GEFLLLEWLAM | 791 | 222 |
| HLA-B*15:03 | AAVGELLLL | AAVGEFLLL | 1475 | 426 |
| HLA-A*02:01 | QLQAAVGELLL | QLQAAVGEFLL | 1401 | 441 |
| HLA-A*02:02 | LLLLEWLAMAV | FLLLEWLAMAV | 453 | 147 |
| HLA-C*03:03 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-C*03:04 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-A*02:02 | QLQAAVGELLL | QLQAAVGEFLL | 530 | 186 |
| HLA-B*50:01 | GELLLLEWLA | GEFLLLEWLA | 1298 | 484 |
| HLA-A*02:11 | LLLLEWLAMA | FLLLEWLAMA | 8 | 3 |
| HLA-A*02:01 | LQAAVGELLL | LQAAVGEFLL | 917 | 347 |
| HLA-C*03:03 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-C*03:04 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-A*02:06 | LQAAVGELLL | LQAAVGEFLL | 162 | 63 |
| HLA-B*40:06 | GELLLLEWLA | GEFLLLEWLA | 268 | 106 |
| HLA-B*40:02 | GELLLLEWLAM | GEFLLLEWLAM | 201 | 83 |
| HLA-A*02:02 | LQAAVGELLL | LQAAVGEFLL | 100 | 42 |
| HLA-C*03:02 | AAVGELLLL | AAVGEFLLL | 269 | 116 |
| HLA-A*02:01 | LLLLEWLAMAV | FLLLEWLAMAV | 97 | 44 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVGEFL | 303 | 139 |
| HLA-B*15:01 | LQAAVGELLL | LQAAVGEFLL | 109 | 237 |
| HLA-B*15:03 | LQAAVGELLL | LQAAVGEFLL | 98 | 210 |
| HLA-A*02:11 | LQAAVGELL | LQAAVGEFL | 386 | 183 |
| HLA-B*40:02 | GELLLLEWLAMAV | GEFLLLEWLAMAV | 815 | 391 |
| HLA-B*40:02 | GELLLLEWLA | GEFLLLEWLA | 475 | 231 |
| HLA-A*02:06 | IQLQAAVGELLL | IQLQAAVGEFLL | 626 | 310 |
| HLA-C*16:01 | AAVGELLLL | AAVGEFLLL | 205 | 102 |
NSP3 protein
T1189I
Reference GAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSED
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated GAKLLHKPIVWHVNNATNKATYKPNIWCIRCLWSTKPVETSNSFDVLKSED
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*57:01 | NTWCIRCLW | NIWCIRCLW | 17 | 423 |
| HLA-B*57:01 | YKPNTWCIRCLW | YKPNIWCIRCLW | 105 | 2607 |
| HLA-B*58:01 | NTWCIRCLW | NIWCIRCLW | 16 | 325 |
| HLA-B*57:01 | KPNTWCIRCLW | KPNIWCIRCLW | 28 | 517 |
| HLA-B*57:01 | TYKPNTWCIRCLW | TYKPNIWCIRCLW | 266 | 4183 |
| HLA-B*57:01 | PNTWCIRCLW | PNIWCIRCLW | 142 | 1929 |
| HLA-B*58:01 | YKPNTWCIRCLW | YKPNIWCIRCLW | 231 | 3000 |
| HLA-B*58:01 | KPNTWCIRCLW | KPNIWCIRCLW | 68 | 781 |
| HLA-B*57:01 | TWCIRCLW | IWCIRCLW | 460 | 3614 |
| HLA-B*58:01 | PNTWCIRCLW | PNIWCIRCLW | 394 | 2545 |
| HLA-A*68:01 | NTWCIRCLWSTK | NIWCIRCLWSTK | 67 | 375 |
| HLA-A*11:01 | NTWCIRCLWSTK | NIWCIRCLWSTK | 442 | 2283 |
| HLA-A*30:01 | NTWCIRCLWSTK | NIWCIRCLWSTK | 403 | 1527 |
| HLA-A*32:01 | NTWCIRCLW | NIWCIRCLW | 142 | 357 |
| HLA-A*31:01 | NTWCIRCLWSTK | NIWCIRCLWSTK | 313 | 707 |
| HLA-A*02:11 | ATYKPNTWCI | ATYKPNIWCI | 308 | 139 |
| HLA-A*02:06 | ATYKPNTWCI | ATYKPNIWCI | 645 | 304 |
| HLA-A*32:01 | KPNTWCIRCLW | KPNIWCIRCLW | 477 | 991 |
NSP6 protein
SGF 106-108 deletion
Reference FNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FNMVYMPASWVMRIMTWLDMVDTSL‑‑‑KLKDCVMYASAVVLLILMTARTVYD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | FKLKDCVMYA | - | 8 | - |
| HLA-A*02:11 | FKLKDCVMYA | - | 12 | - |
| HLA-A*68:01 | MVDTSLSGFK | MVDTSLK | 13 | - |
| HLA-B*15:03 | FKLKDCVMY | - | 15 | - |
| HLA-A*11:01 | MVDTSLSGFK | MVDTSLK | 21 | - |
| HLA-A*11:01 | TSLSGFKLK | TSLKLK | 22 | - |
| HLA-A*03:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 25 |
| HLA-A*02:01 | FKLKDCVMYA | - | 27 | - |
| HLA-A*68:01 | DTSLSGFKLK | DTSLKLK | 33 | - |
| HLA-A*03:02 | MVDTSLSGFK | MVDTSLK | 35 | - |
| HLA-A*68:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 37 |
| HLA-A*03:01 | MVDTSLSGFK | MVDTSLK | 54 | - |
| HLA-A*03:02 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 59 |
| HLA-C*05:01 | MVDTSLSGF | MVDTSL | 64 | - |
| HLA-A*11:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 71 |
| HLA-A*02:06 | FKLKDCVMYA | - | 90 | - |
| HLA-A*30:01 | TSLSGFKLK | TSLKLK | 107 | - |
| HLA-A*02:02 | SGFKLKDCVMYA | - | 126 | - |
| HLA-A*11:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 128 |
| HLA-A*03:02 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 147 |
| HLA-C*08:02 | MVDTSLSGF | MVDTSL | 159 | - |
| HLA-A*03:02 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 168 |
| HLA-A*02:11 | SGFKLKDCVMYA | - | 200 | - |
| HLA-A*02:02 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 202 |
| HLA-A*01:01 | MVDTSLSGFKLKDCVMY | MVDTSLKLKDCVMY | - | 207 |
| HLA-C*03:02 | MVDTSLSGF | MVDTSL | 211 | - |
| HLA-A*02:02 | GFKLKDCVMYA | - | 239 | - |
| HLA-A*02:02 | TSLSGFKLKDCVMYA | TSLKLKDCVMYA | - | 244 |
| HLA-A*03:01 | TSLSGFKLK | TSLKLK | 244 | - |
| HLA-B*15:03 | MVDTSLSGF | MVDTSL | 276 | - |
| HLA-A*68:01 | TSLSGFKLK | TSLKLK | 286 | - |
| HLA-A*03:02 | TSLSGFKLK | TSLKLK | 289 | - |
| HLA-A*03:01 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 309 |
| HLA-A*68:02 | DTSLSGFKL | DTSLKL | 313 | - |
| HLA-A*02:11 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 346 |
| HLA-B*15:01 | DMVDTSLSGF | DMVDTSL | 362 | - |
| HLA-A*23:01 | TWLDMVDTSLSGF | - | 387 | - |
| HLA-A*02:11 | GFKLKDCVMYA | - | 394 | - |
| HLA-A*03:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 415 |
| HLA-A*26:01 | DMVDTSLSGF | DMVDTSL | 417 | - |
| HLA-B*08:01 | MVDTSLSGFKLKDCVM | MVDTSLKLKDCVM | - | 426 |
| HLA-A*02:02 | WLDMVDTSLS | - | 441 | - |
| HLA-A*02:11 | FKLKDCVMYASAVV | - | 481 | - |
| HLA-A*11:01 | DTSLSGFKLK | DTSLKLK | 488 | - |
| HLA-B*35:01 | MVDTSLSGF | MVDTSL | 490 | - |
| HLA-A*31:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 493 |
| HLA-B*15:03 | LSGFKLKDCVMY | LKLKDCVMY | 3466 | 22 |
| HLA-A*02:11 | LDMVDTSLSGFKL | LDMVDTSLKL | 16396 | 166 |
| HLA-A*02:02 | LDMVDTSLSGFKL | LDMVDTSLKL | 9971 | 106 |
| HLA-A*02:11 | SLSGFKLKDCV | SLKLKDCV | 122 | 9934 |
| HLA-A*02:01 | LDMVDTSLSGFKL | LDMVDTSLKL | 20579 | 311 |
| HLA-B*08:01 | SLSGFKLKDCVM | SLKLKDCVM | 929 | 36 |
| HLA-A*02:02 | SLSGFKLKDCV | SLKLKDCV | 408 | 10216 |
| HLA-C*05:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 8375 | 417 |
| HLA-B*15:01 | SLSGFKLKDCVMY | SLKLKDCVMY | 2952 | 158 |
| HLA-B*08:01 | TSLSGFKLKDCVM | TSLKLKDCVM | 4628 | 289 |
| HLA-B*15:01 | SLSGFKLKDCVM | SLKLKDCVM | 2654 | 197 |
| HLA-A*68:01 | DMVDTSLSGFK | DMVDTSLK | 366 | 4919 |
| HLA-A*02:11 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 1342 | 108 |
| HLA-C*08:02 | MVDTSLSGFKL | MVDTSLKL | 2134 | 174 |
| HLA-B*15:03 | SLSGFKLKDCVM | SLKLKDCVM | 3634 | 297 |
| HLA-A*02:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 2214 | 195 |
| HLA-A*03:02 | WLDMVDTSLSGFK | WLDMVDTSLK | 1756 | 156 |
| HLA-A*02:02 | LSGFKLKDCVMYA | LKLKDCVMYA | 305 | 28 |
| HLA-A*02:01 | LSGFKLKDCVMYA | LKLKDCVMYA | 1788 | 165 |
| HLA-A*02:11 | LSGFKLKDCVMYA | LKLKDCVMYA | 578 | 54 |
| HLA-B*15:03 | SLSGFKLKDCVMY | SLKLKDCVMY | 3626 | 349 |
| HLA-A*03:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 2900 | 287 |
| HLA-C*05:01 | MVDTSLSGFKL | MVDTSLKL | 1125 | 124 |
| HLA-A*11:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 4020 | 492 |
| HLA-A*02:02 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 788 | 116 |
| HLA-A*68:01 | DMVDTSLSGFKLK | DMVDTSLKLK | 1679 | 251 |
| HLA-A*02:01 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 203 | 853 |
| HLA-A*02:11 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 64 | 223 |
| HLA-A*02:02 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 55 | 171 |
| HLA-A*29:02 | SLSGFKLKDCVMY | SLKLKDCVMY | 404 | 1192 |
| HLA-A*11:01 | MVDTSLSGFKLK | MVDTSLKLK | 143 | 50 |
| HLA-A*03:02 | MVDTSLSGFKLK | MVDTSLKLK | 312 | 127 |
| HLA-A*68:01 | MVDTSLSGFKLK | MVDTSLKLK | 602 | 246 |
| HLA-A*03:01 | MVDTSLSGFKLK | MVDTSLKLK | 441 | 190 |
NSP12 protein
P323F
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPFTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPF | 3115 | 4 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPF | 5415 | 8 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPF | 5731 | 11 |
| HLA-A*23:01 | VLFSTVFPP | VLFSTVFPF | 17486 | 35 |
| HLA-A*29:02 | VLFSTVFPP | VLFSTVFPF | 13973 | 41 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPF | 20533 | 102 |
| HLA-B*15:01 | NVLFSTVFPP | NVLFSTVFPF | 16240 | 100 |
| HLA-B*15:03 | NVLFSTVFPP | NVLFSTVFPF | 20337 | 128 |
| HLA-A*24:02 | VLFSTVFPP | VLFSTVFPF | 25301 | 183 |
| HLA-B*35:01 | VLFSTVFPP | VLFSTVFPF | 21745 | 165 |
| HLA-B*15:03 | FNVLFSTVFPP | FNVLFSTVFPF | 16079 | 155 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPF | 11979 | 160 |
| HLA-A*29:02 | NVLFSTVFPP | NVLFSTVFPF | 21903 | 310 |
| HLA-A*68:01 | PTSFGPLVR | FTSFGPLVR | 908 | 13 |
| HLA-A*32:01 | NVLFSTVFPP | NVLFSTVFPF | 21364 | 323 |
| HLA-A*23:01 | NFNVLFSTVFPP | NFNVLFSTVFPF | 16444 | 252 |
| HLA-B*15:01 | FNVLFSTVFPP | FNVLFSTVFPF | 21004 | 331 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPF | 21407 | 342 |
| HLA-A*23:01 | LFSTVFPP | LFSTVFPF | 24191 | 413 |
| HLA-B*15:03 | LFSTVFPP | LFSTVFPF | 23012 | 399 |
| HLA-B*35:01 | NVLFSTVFPP | NVLFSTVFPF | 23419 | 426 |
| HLA-A*29:02 | NFNVLFSTVFPP | NFNVLFSTVFPF | 21013 | 409 |
| HLA-B*35:01 | FPPTSFGPL | FPFTSFGPL | 304 | 6 |
| HLA-B*53:01 | FPPTSFGPL | FPFTSFGPL | 3581 | 84 |
| HLA-B*35:01 | FPPTSFGPLVRKIF | FPFTSFGPLVRKIF | 7080 | 191 |
| HLA-C*03:02 | PTSFGPLVRKIF | FTSFGPLVRKIF | 14624 | 448 |
| HLA-A*68:01 | PTSFGPLVRK | FTSFGPLVRK | 308 | 11 |
| HLA-A*68:01 | PPTSFGPLVR | PFTSFGPLVR | 4035 | 158 |
| HLA-A*33:03 | PTSFGPLVR | FTSFGPLVR | 3228 | 161 |
| HLA-B*35:03 | FPPTSFGPL | FPFTSFGPL | 286 | 16 |
| HLA-B*15:03 | FPPTSFGPL | FPFTSFGPL | 4804 | 375 |
| HLA-A*68:02 | PTSFGPLVRKIFV | FTSFGPLVRKIFV | 1635 | 142 |
| HLA-C*01:02 | FPPTSFGPL | FPFTSFGPL | 217 | 2475 |
| HLA-B*51:01 | FPPTSFGPL | FPFTSFGPL | 1809 | 165 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPFTSFGPL | 777 | 78 |
| HLA-A*68:01 | PPTSFGPLVRK | PFTSFGPLVRK | 4812 | 493 |
| HLA-A*11:01 | PTSFGPLVR | FTSFGPLVR | 4068 | 420 |
| HLA-C*03:02 | FPPTSFGPL | FPFTSFGPL | 960 | 101 |
| HLA-A*31:01 | PTSFGPLVR | FTSFGPLVR | 4402 | 481 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPFTSFGPL | 1320 | 145 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPFTSFGPL | 1498 | 175 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPFTSFGPL | 738 | 87 |
| HLA-A*26:01 | STVFPPTSF | STVFPFTSF | 786 | 95 |
| HLA-A*03:02 | PTSFGPLVRK | FTSFGPLVRK | 868 | 107 |
| HLA-C*03:03 | FPPTSFGPL | FPFTSFGPL | 375 | 51 |
| HLA-C*03:04 | FPPTSFGPL | FPFTSFGPL | 375 | 51 |
| HLA-B*07:02 | VFPPTSFGPL | VFPFTSFGPL | 339 | 47 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPFTSFGPL | 843 | 119 |
| HLA-B*42:01 | LFSTVFPPTSFGPL | LFSTVFPFTSFGPL | 3265 | 483 |
| HLA-A*03:01 | PTSFGPLVRK | FTSFGPLVRK | 554 | 82 |
| HLA-B*42:01 | VFPPTSFGPL | VFPFTSFGPL | 274 | 43 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPFTSFGPL | 2537 | 435 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPFTSFGPLV | 789 | 137 |
| HLA-A*11:01 | PTSFGPLVRK | FTSFGPLVRK | 123 | 24 |
| HLA-B*07:02 | FPPTSFGPLV | FPFTSFGPLV | 1131 | 232 |
| HLA-B*42:01 | FPPTSFGPLV | FPFTSFGPLV | 368 | 76 |
| HLA-B*08:01 | FPPTSFGPL | FPFTSFGPL | 973 | 203 |
| HLA-B*07:02 | FPPTSFGPL | FPFTSFGPL | 42 | 9 |
| HLA-B*15:01 | STVFPPTSF | STVFPFTSF | 130 | 28 |
| HLA-A*25:01 | STVFPPTSF | STVFPFTSF | 1533 | 348 |
| HLA-A*02:01 | TVFPPTSFGPLV | TVFPFTSFGPLV | 1358 | 315 |
| HLA-B*51:01 | FPPTSFGPLV | FPFTSFGPLV | 363 | 86 |
| HLA-B*42:01 | FPPTSFGPL | FPFTSFGPL | 21 | 5 |
| HLA-A*68:02 | FPPTSFGPL | FPFTSFGPL | 1395 | 346 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPF | 78 | 20 |
| HLA-A*32:01 | STVFPPTSF | STVFPFTSF | 230 | 59 |
| HLA-C*12:03 | FPPTSFGPL | FPFTSFGPL | 1422 | 385 |
| HLA-A*02:11 | TVFPPTSFGPLV | TVFPFTSFGPLV | 306 | 86 |
| HLA-A*31:01 | STVFPPTSFGPLVR | STVFPFTSFGPLVR | 1475 | 417 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPFTSFGPLV | 169 | 48 |
| HLA-A*02:06 | TVFPPTSFGPLV | TVFPFTSFGPLV | 277 | 79 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPFTSF | 1667 | 479 |
| HLA-C*16:01 | FPPTSFGPL | FPFTSFGPL | 993 | 296 |
| HLA-A*68:02 | TVFPPTSFG | TVFPFTSFG | 443 | 133 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPFTSF | 88 | 27 |
| HLA-A*68:02 | FPPTSFGPLV | FPFTSFGPLV | 991 | 312 |
| HLA-A*02:06 | TVFPPTSFGPL | TVFPFTSFGPL | 1253 | 414 |
| HLA-B*15:03 | STVFPPTSF | STVFPFTSF | 60 | 20 |
| HLA-B*57:01 | FSTVFPPTSF | FSTVFPFTSF | 648 | 220 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPF | 368 | 126 |
| HLA-B*53:01 | FPPTSFGPLV | FPFTSFGPLV | 767 | 282 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPF | 413 | 155 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPF | 269 | 106 |
| HLA-A*31:01 | TVFPPTSFGPLVR | TVFPFTSFGPLVR | 106 | 42 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPF | 739 | 295 |
| HLA-C*14:02 | FPPTSFGPL | FPFTSFGPL | 255 | 104 |
| HLA-B*15:03 | TVFPPTSF | TVFPFTSF | 465 | 196 |
| HLA-A*33:03 | STVFPPTSFGPLVR | STVFPFTSFGPLVR | 671 | 284 |
| HLA-C*12:02 | STVFPPTSF | STVFPFTSF | 320 | 147 |
| HLA-B*15:01 | FSTVFPPTSF | FSTVFPFTSF | 150 | 69 |
| HLA-A*68:02 | TVFPPTSFGPL | TVFPFTSFGPL | 143 | 67 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPF | 179 | 87 |