Summary
This strain contains 20 protein-level mutations:
- 6 mutations in Spike protein: L5F, HV 69-70 deletion, T95I, D614G, T859N, D950H
- 2 mutations in N protein: T205I, M234I
- 2 mutations in NS3 protein: P42L, Q57H
- 2 mutations in NS8 protein: T11I, A51S
- 1 mutation in NS9c protein: L52F
- 1 mutation in NSP2 protein: T85I
- 3 mutations in NSP4 protein: K399E, L438P, A446V
- 2 mutations in NSP6 protein: SGF 106-108 deletion, V278I
- 1 mutation in NSP12 protein: P323L
We identified all possible linear viral peptides affected by these mutations. Whenever it was possible, we matched the reference peptide with the mutated one. For example, D -> L mutation transformed SDNGPQNQR to SLNGPQNQR. Cases when it was not meaningful included deletions and insertions at the flanks of the peptide, e.g., HV deletion in NVTWFHAIHV peptide.
Then, we predicted binding affinities between the selected peptides and frequent HLA alleles. Predictions were made with NetMHCpan-4.1 and NetMHCIIpan-4.0. The binding affinities were classifies into three groups:
- Tight binding (IC50 affinity ≤ 50 nM)
- Moderate binding (50 nM < IC50 affinity ≤ 500 nM)
- Weak/no binding (IC50 affinity > 500 nM)
Here we report HLA-peptide interactions whose affinity was altered by at least two folds. Note that mutations with empty set of altered interactions are not showed.
The total number of HLA-peptide interactions affected by the mutations
For each allele we calculated the number of peptides with increased and decreased affinitiy (two or more times). The absolute number of such peptides is showed on the left side of the figure (blue bars — weaker binding, orange bars — stronger).
While absolute numbers are of interest, they do not reflect the initial number of tighly binding peptides for the specific allele. Roughly speaking, if an allele had high number of tight binders, then vanished affinity of several peptides will not affect much the integral ability of peptide presentation. To the contrary, low number of vanished peptides could be important if the particular allele initially had narrow epitope repertoire. To account for this effect, we normalized the absolute numbers of new/vanished tight binders (IC50 affinity ≤ 50 nM) by the total number of tight binders for each allele. To avoid possible divisions by zero, we used + 1 regularization term in all denominators. For example, if some allele had no tight binders in the reference genome, and three new tight binders appeared as a result of the mutation, the relative increase will be 300%. The results are showed in the right part of the figure. Protein-specific plots are listed below.
Spike protein
L5F
Reference MFVFLVLLPLVSSQCVNLTTRTQLPPAYTN
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Mutated MFVFFVLLPLVSSQCVNLTTRTQLPPAYTN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | FLVLLPLV | FFVLLPLV | 34 | 5226 |
| HLA-A*02:01 | FLVLLPLV | FFVLLPLV | 83 | 8821 |
| HLA-A*02:02 | FLVLLPLV | FFVLLPLV | 139 | 7441 |
| HLA-A*02:06 | FLVLLPLV | FFVLLPLV | 144 | 4618 |
| HLA-A*02:02 | FLVLLPLVSS | FFVLLPLVSS | 379 | 5854 |
| HLA-A*02:11 | FLVLLPLVSS | FFVLLPLVSS | 340 | 5067 |
| HLA-A*02:01 | FLVLLPLVSS | FFVLLPLVSS | 500 | 6333 |
| HLA-A*02:11 | FLVLLPLVSSQCV | FFVLLPLVSSQCV | 134 | 1444 |
| HLA-A*02:02 | FLVLLPLVSSQCV | FFVLLPLVSSQCV | 484 | 4599 |
| HLA-A*02:01 | FLVLLPLVSSQCV | FFVLLPLVSSQCV | 462 | 4095 |
| HLA-A*02:06 | LVLLPLVSS | FVLLPLVSS | 1562 | 285 |
| HLA-A*68:02 | FVFLVLLPL | FVFFVLLPL | 212 | 40 |
| HLA-A*68:02 | MFVFLVLLPL | MFVFFVLLPL | 354 | 78 |
| HLA-A*68:02 | FVFLVLLPLV | FVFFVLLPLV | 128 | 32 |
| HLA-A*02:06 | LVLLPLVSSQCV | FVLLPLVSSQCV | 656 | 174 |
| HLA-A*02:02 | FVFLVLLPL | FVFFVLLPL | 132 | 44 |
| HLA-A*02:01 | FVFLVLLPL | FVFFVLLPL | 125 | 46 |
| HLA-C*14:02 | VFLVLLPL | VFFVLLPL | 782 | 313 |
| HLA-A*02:11 | FVFLVLLPL | FVFFVLLPL | 17 | 7 |
| HLA-A*02:11 | MFVFLVLLPL | MFVFFVLLPL | 269 | 116 |
| HLA-A*02:06 | FVFLVLLPL | FVFFVLLPL | 67 | 30 |
| HLA-A*02:02 | FVFLVLLPLV | FVFFVLLPLV | 104 | 48 |
| HLA-A*02:11 | LVLLPLVSSQCV | FVLLPLVSSQCV | 290 | 135 |
| HLA-C*12:03 | FVFLVLLPL | FVFFVLLPL | 215 | 102 |
| HLA-C*12:02 | FVFLVLLPL | FVFFVLLPL | 609 | 292 |
| HLA-A*02:02 | MFVFLVLLPL | MFVFFVLLPL | 395 | 193 |
HV 69-70 deletion
Reference RSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAST
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Mutated RSSVLHSTQDLFLPFFSNVTWFHAI‑‑SGTNGTKRFDNPVLPFNDGVYFASI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | NVTWFHAIHV | - | 16 | - |
| HLA-A*02:11 | VTWFHAIHV | VTWFHAI | 18 | - |
| HLA-A*02:06 | VTWFHAIHV | VTWFHAI | 40 | - |
| HLA-A*68:01 | HVSGTNGTKR | - | 42 | - |
| HLA-A*68:02 | VTWFHAIHV | VTWFHAI | 45 | - |
| HLA-A*68:01 | HVSGTNGTK | SGTNGTK | 54 | - |
| HLA-C*15:02 | VTWFHAIHV | VTWFHAI | 107 | - |
| HLA-A*02:11 | NVTWFHAIHV | - | 125 | - |
| HLA-A*02:01 | VTWFHAIHV | VTWFHAI | 134 | - |
| HLA-A*02:11 | FFSNVTWFHAIHV | - | 219 | - |
| HLA-A*02:11 | FSNVTWFHAIHV | - | 250 | - |
| HLA-A*02:06 | NVTWFHAIHV | - | 263 | - |
| HLA-A*68:02 | FSNVTWFHAIHV | - | 311 | - |
| HLA-A*33:03 | WFHAIHVSGTNGTKR | WFHAISGTNGTKR | - | 321 |
| HLA-C*12:03 | VTWFHAIHV | VTWFHAI | 341 | - |
| HLA-A*02:02 | VTWFHAIHV | VTWFHAI | 369 | - |
| HLA-C*16:01 | VTWFHAIHV | VTWFHAI | 421 | - |
| HLA-A*32:01 | VTWFHAIHV | VTWFHAI | 442 | - |
| HLA-A*02:06 | FSNVTWFHAIHV | - | 449 | - |
| HLA-A*02:02 | NVTWFHAIHV | - | 468 | - |
| HLA-A*68:02 | FFSNVTWFHAIHV | - | 492 | - |
| HLA-A*11:01 | HAIHVSGTNGTK | HAISGTNGTK | 2998 | 438 |
| HLA-A*03:02 | AIHVSGTNGTK | AISGTNGTK | 1179 | 271 |
| HLA-A*68:01 | HAIHVSGTNGTK | HAISGTNGTK | 174 | 43 |
| HLA-A*11:01 | AIHVSGTNGTK | AISGTNGTK | 742 | 223 |
| HLA-A*03:01 | AIHVSGTNGTK | AISGTNGTK | 534 | 196 |
| HLA-A*33:03 | HAIHVSGTNGTKR | HAISGTNGTKR | 374 | 755 |
T95I
Reference VSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIV
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Mutated ‑SGTNGTKRFDNPVLPFNDGVYFASIEKSNIIRGWIFGTTLDSKTQSLLIV
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*57:01 | STEKSNIIRGW | SIEKSNIIRGW | 135 | 2014 |
| HLA-B*58:01 | STEKSNIIRGW | SIEKSNIIRGW | 183 | 2582 |
| HLA-B*42:01 | LPFNDGVYFAST | LPFNDGVYFASI | 4329 | 325 |
| HLA-B*53:01 | LPFNDGVYFAST | LPFNDGVYFASI | 6125 | 497 |
| HLA-C*12:03 | ASTEKSNII | ASIEKSNII | 2443 | 439 |
| HLA-A*31:01 | ASTEKSNIIR | ASIEKSNIIR | 1324 | 354 |
| HLA-C*03:03 | FASTEKSNII | FASIEKSNII | 1633 | 453 |
| HLA-C*03:04 | FASTEKSNII | FASIEKSNII | 1633 | 453 |
| HLA-A*68:01 | ASTEKSNIIR | ASIEKSNIIR | 1387 | 410 |
| HLA-C*12:03 | FASTEKSNII | FASIEKSNII | 902 | 268 |
| HLA-A*11:01 | ASTEKSNIIR | ASIEKSNIIR | 1555 | 495 |
| HLA-C*15:02 | ASTEKSNII | ASIEKSNII | 729 | 264 |
| HLA-B*44:02 | TEKSNIIRGW | IEKSNIIRGW | 20 | 55 |
| HLA-B*44:03 | TEKSNIIRGW | IEKSNIIRGW | 33 | 84 |
| HLA-C*16:01 | ASTEKSNII | ASIEKSNII | 603 | 239 |
| HLA-A*68:01 | STEKSNIIR | SIEKSNIIR | 186 | 458 |
| HLA-A*33:03 | YFASTEKSNIIR | YFASIEKSNIIR | 337 | 148 |
| HLA-A*11:01 | GVYFASTEKS | GVYFASIEKS | 648 | 298 |
| HLA-A*11:01 | DGVYFASTEK | DGVYFASIEK | 205 | 96 |
| HLA-B*58:01 | ASTEKSNIIRGW | ASIEKSNIIRGW | 245 | 122 |
D614G
Reference PCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTG
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Mutated PCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTG
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*05:01 | YQDVNCTEV | YQGVNCTEV | 82 | 8597 |
| HLA-C*08:02 | YQDVNCTEV | YQGVNCTEV | 89 | 8066 |
| HLA-A*02:11 | DVNCTEVPV | GVNCTEVPV | 2140 | 72 |
| HLA-A*02:02 | DVNCTEVPV | GVNCTEVPV | 4099 | 213 |
| HLA-A*68:02 | DVNCTEVPV | GVNCTEVPV | 36 | 659 |
| HLA-A*02:06 | DVNCTEVPV | GVNCTEVPV | 3807 | 208 |
| HLA-A*68:02 | QVAVLYQDV | QVAVLYQGV | 183 | 13 |
| HLA-A*68:02 | NQVAVLYQDV | NQVAVLYQGV | 650 | 52 |
| HLA-A*02:06 | QVAVLYQDV | QVAVLYQGV | 1312 | 148 |
| HLA-A*02:11 | QVAVLYQDV | QVAVLYQGV | 2169 | 268 |
| HLA-A*68:02 | NTSNQVAVLYQDV | NTSNQVAVLYQGV | 1858 | 231 |
| HLA-A*68:02 | TSNQVAVLYQDV | TSNQVAVLYQGV | 3281 | 457 |
| HLA-A*02:02 | QVAVLYQDV | QVAVLYQGV | 1107 | 188 |
| HLA-A*02:01 | YQDVNCTEV | YQGVNCTEV | 75 | 315 |
| HLA-A*02:06 | YQDVNCTEV | YQGVNCTEV | 17 | 57 |
| HLA-A*02:11 | VLYQDVNCT | VLYQGVNCT | 224 | 74 |
| HLA-A*02:11 | YQDVNCTEV | YQGVNCTEV | 31 | 93 |
| HLA-A*02:11 | VLYQDVNCTEVP | VLYQGVNCTEVP | 1000 | 402 |
| HLA-A*02:02 | NQVAVLYQDV | NQVAVLYQGV | 269 | 116 |
| HLA-A*02:11 | AVLYQDVNCTEV | AVLYQGVNCTEV | 500 | 238 |
| HLA-A*02:01 | NQVAVLYQDV | NQVAVLYQGV | 558 | 267 |
| HLA-A*02:06 | NQVAVLYQDV | NQVAVLYQGV | 36 | 18 |
T859N
Reference IKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITS
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Mutated IKQYGDCLGDIAARDLICAQKFNGLNVLPPLLTDEMIAQYTSALLAGTITS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | AQKFNGLTV | AQKFNGLNV | 65 | 252 |
| HLA-B*15:01 | AQKFNGLTV | AQKFNGLNV | 210 | 628 |
| HLA-A*02:11 | FNGLTVLPPLL | FNGLNVLPPLL | 171 | 68 |
| HLA-A*02:11 | GLTVLPPLL | GLNVLPPLL | 29 | 14 |
D950H
Reference NQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISS
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Mutated NQFNSAIGKIQDSLSSTASALGKLQHVVNQNAQALNTLVKQLSSNFGAISS
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | ALGKLQDVV | ALGKLQHVV | 103 | 12 |
| HLA-A*02:11 | ALGKLQDV | ALGKLQHV | 2627 | 370 |
| HLA-A*02:02 | KLQDVVNQNAQAL | KLQHVVNQNAQAL | 81 | 495 |
| HLA-A*02:11 | KLQDVVNQNAQAL | KLQHVVNQNAQAL | 71 | 341 |
| HLA-A*02:11 | KLQDVVNQNAQA | KLQHVVNQNAQA | 95 | 455 |
| HLA-A*02:02 | KLQDVVNQNAQA | KLQHVVNQNAQA | 318 | 1510 |
| HLA-A*02:11 | SALGKLQDVV | SALGKLQHVV | 1227 | 264 |
| HLA-A*02:01 | KLQDVVNQNAQAL | KLQHVVNQNAQAL | 435 | 2021 |
| HLA-A*02:11 | SALGKLQDV | SALGKLQHV | 475 | 104 |
| HLA-A*02:06 | SALGKLQDV | SALGKLQHV | 258 | 60 |
| HLA-A*02:01 | ALGKLQDVV | ALGKLQHVV | 852 | 203 |
| HLA-A*02:11 | KLQDVVNQNA | KLQHVVNQNA | 56 | 228 |
| HLA-A*02:02 | KLQDVVNQNA | KLQHVVNQNA | 129 | 519 |
| HLA-C*12:03 | SALGKLQDV | SALGKLQHV | 197 | 54 |
| HLA-C*15:02 | SALGKLQDV | SALGKLQHV | 566 | 182 |
| HLA-A*02:02 | ALGKLQDVV | ALGKLQHVV | 129 | 48 |
| HLA-A*68:02 | STASALGKLQDV | STASALGKLQHV | 442 | 168 |
| HLA-B*15:03 | LQDVVNQNAQAL | LQHVVNQNAQAL | 1044 | 416 |
| HLA-C*16:01 | SALGKLQDV | SALGKLQHV | 160 | 70 |
| HLA-A*68:02 | STASALGKLQDVV | STASALGKLQHVV | 414 | 202 |
N protein
T205I
Reference SQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQL
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Mutated SQASSRSSSRSRNSSRNSTPGSSRGISPARMAGNGGDAALALLLLDRLNQL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-C*01:02 | STPGSSRGT | STPGSSRGI | 10810 | 468 |
| HLA-A*30:01 | GTSPARMAG | GISPARMAG | 493 | 1901 |
| HLA-A*68:01 | SSRGTSPAR | SSRGISPAR | 186 | 61 |
| HLA-A*68:01 | STPGSSRGTSPAR | STPGSSRGISPAR | 860 | 288 |
| HLA-A*30:01 | RGTSPARMA | RGISPARMA | 1160 | 422 |
| HLA-A*68:01 | GSSRGTSPAR | GSSRGISPAR | 615 | 248 |
| HLA-A*31:01 | GSSRGTSPAR | GSSRGISPAR | 262 | 111 |
| HLA-A*33:03 | SSRGTSPAR | SSRGISPAR | 106 | 46 |
| HLA-A*33:03 | STPGSSRGTSPAR | STPGSSRGISPAR | 968 | 473 |
M234I
Reference RMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPR
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Mutated RMAGNGGDAALALLLLDRLNQLESKISGKGQQQQGQTVTKKSAAEASKKPR
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:03 | RLNQLESKM | RLNQLESKI | 210 | 3572 |
| HLA-B*15:01 | RLNQLESKM | RLNQLESKI | 434 | 5192 |
| HLA-A*02:11 | LLLDRLNQLESKM | LLLDRLNQLESKI | 1721 | 220 |
| HLA-A*32:01 | RLNQLESKM | RLNQLESKI | 1285 | 261 |
| HLA-A*02:11 | RLNQLESKM | RLNQLESKI | 584 | 173 |
NS3 protein
P42L
Reference QGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKK
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Mutated QGEIKDATPSDFVRATATIPIQASLLFGWLIVGVALLAVFHSASKIITLKK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | LPFGWLIVGV | LLFGWLIVGV | 4732 | 8 |
| HLA-A*02:11 | LPFGWLIVGV | LLFGWLIVGV | 1743 | 3 |
| HLA-A*02:11 | LPFGWLIVGVAL | LLFGWLIVGVAL | 14245 | 29 |
| HLA-A*02:02 | LPFGWLIVGV | LLFGWLIVGV | 3442 | 11 |
| HLA-A*02:11 | LPFGWLIVGVA | LLFGWLIVGVA | 18600 | 65 |
| HLA-C*01:02 | TIPIQASLP | TIPIQASLL | 25201 | 102 |
| HLA-B*42:01 | LPFGWLIVGVAL | LLFGWLIVGVAL | 62 | 14834 |
| HLA-A*02:11 | LPFGWLIV | LLFGWLIV | 9504 | 41 |
| HLA-B*35:01 | LPFGWLIVG | LLFGWLIVG | 247 | 27950 |
| HLA-B*35:01 | LPFGWLIVGVAL | LLFGWLIVGVAL | 286 | 31951 |
| HLA-B*51:01 | LPFGWLIV | LLFGWLIV | 245 | 26530 |
| HLA-A*02:06 | LPFGWLIVGV | LLFGWLIVGV | 2421 | 23 |
| HLA-A*02:11 | LPFGWLIVG | LLFGWLIVG | 23342 | 241 |
| HLA-A*02:02 | LPFGWLIVGVAL | LLFGWLIVGVAL | 19947 | 226 |
| HLA-A*02:01 | LPFGWLIVGVAL | LLFGWLIVGVAL | 18634 | 236 |
| HLA-A*02:01 | LPFGWLIVGVA | LLFGWLIVGVA | 23543 | 342 |
| HLA-B*07:02 | LPFGWLIVGVAL | LLFGWLIVGVAL | 411 | 27790 |
| HLA-A*02:11 | LPFGWLIVGVALLA | LLFGWLIVGVALLA | 12056 | 210 |
| HLA-A*02:01 | LPFGWLIV | LLFGWLIV | 15645 | 295 |
| HLA-A*02:11 | LPFGWLIVGVALL | LLFGWLIVGVALL | 980 | 20 |
| HLA-A*02:01 | LPFGWLIVGVALL | LLFGWLIVGVALL | 2106 | 68 |
| HLA-B*42:01 | IPIQASLP | IPIQASLL | 6612 | 226 |
| HLA-A*02:02 | LPFGWLIVGVALL | LLFGWLIVGVALL | 762 | 31 |
| HLA-A*02:11 | PFGWLIVGV | LFGWLIVGV | 7794 | 398 |
| HLA-C*15:02 | ASLPFGWLI | ASLLFGWLI | 110 | 2004 |
| HLA-A*68:02 | ATIPIQASLP | ATIPIQASLL | 3074 | 222 |
| HLA-B*58:01 | ASLPFGWLI | ASLLFGWLI | 37 | 471 |
| HLA-B*57:01 | ASLPFGWLI | ASLLFGWLI | 116 | 1279 |
| HLA-B*07:02 | ATIPIQASLPF | ATIPIQASLLF | 308 | 3348 |
| HLA-B*07:02 | IPIQASLPF | IPIQASLLF | 24 | 259 |
| HLA-B*07:02 | TIPIQASLPF | TIPIQASLLF | 121 | 1294 |
| HLA-A*02:06 | ASLPFGWLI | ASLLFGWLI | 44 | 447 |
| HLA-B*07:02 | RATATIPIQASLPF | RATATIPIQASLLF | 206 | 2014 |
| HLA-C*15:02 | ASLPFGWLIV | ASLLFGWLIV | 460 | 4426 |
| HLA-B*07:02 | IPIQASLPFGWL | IPIQASLLFGWL | 157 | 1493 |
| HLA-B*58:01 | IQASLPFGWLI | IQASLLFGWLI | 310 | 2878 |
| HLA-A*32:01 | ASLPFGWLI | ASLLFGWLI | 63 | 550 |
| HLA-A*02:11 | ASLPFGWLI | ASLLFGWLI | 73 | 626 |
| HLA-B*42:01 | TIPIQASLPF | TIPIQASLLF | 121 | 888 |
| HLA-B*35:01 | TATIPIQASLPF | TATIPIQASLLF | 153 | 1104 |
| HLA-A*23:01 | ASLPFGWLI | ASLLFGWLI | 330 | 2218 |
| HLA-B*35:01 | TIPIQASLPF | TIPIQASLLF | 27 | 179 |
| HLA-B*42:01 | IPIQASLPF | IPIQASLLF | 20 | 121 |
| HLA-A*02:06 | ASLPFGWLIVGV | ASLLFGWLIVGV | 156 | 941 |
| HLA-A*02:01 | SLPFGWLIV | SLLFGWLIV | 221 | 38 |
| HLA-A*02:11 | SLPFGWLIVGVAL | SLLFGWLIVGVAL | 1004 | 174 |
| HLA-A*02:06 | IQASLPFGWLIV | IQASLLFGWLIV | 239 | 43 |
| HLA-B*35:01 | ATIPIQASLPF | ATIPIQASLLF | 488 | 2676 |
| HLA-A*02:01 | ASLPFGWLI | ASLLFGWLI | 500 | 2741 |
| HLA-B*58:01 | ASLPFGWLIV | ASLLFGWLIV | 475 | 2567 |
| HLA-B*35:01 | IPIQASLPFG | IPIQASLLFG | 290 | 1566 |
| HLA-B*58:01 | RATATIPIQASLPF | RATATIPIQASLLF | 890 | 176 |
| HLA-B*15:01 | TIPIQASLPF | TIPIQASLLF | 32 | 158 |
| HLA-A*02:06 | ASLPFGWLIV | ASLLFGWLIV | 88 | 426 |
| HLA-B*15:01 | ATATIPIQASLPF | ATATIPIQASLLF | 419 | 1991 |
| HLA-B*35:03 | IPIQASLPF | IPIQASLLF | 52 | 242 |
| HLA-B*42:01 | IPIQASLPFGWL | IPIQASLLFGWL | 106 | 491 |
| HLA-A*02:06 | IQASLPFGWL | IQASLLFGWL | 138 | 31 |
| HLA-B*58:01 | QASLPFGWLI | QASLLFGWLI | 81 | 349 |
| HLA-C*03:03 | IPIQASLPF | IPIQASLLF | 406 | 1653 |
| HLA-C*03:04 | IPIQASLPF | IPIQASLLF | 406 | 1653 |
| HLA-B*57:01 | QASLPFGWLI | QASLLFGWLI | 222 | 889 |
| HLA-C*03:02 | TATIPIQASLPF | TATIPIQASLLF | 461 | 1837 |
| HLA-A*02:01 | SLPFGWLIVGV | SLLFGWLIVGV | 170 | 44 |
| HLA-B*15:03 | TIPIQASLPF | TIPIQASLLF | 165 | 618 |
| HLA-A*02:11 | SLPFGWLIVGVALL | SLLFGWLIVGVALL | 622 | 168 |
| HLA-B*15:01 | ATIPIQASLPF | ATIPIQASLLF | 150 | 555 |
| HLA-B*15:03 | TATIPIQASLPF | TATIPIQASLLF | 412 | 1462 |
| HLA-C*14:02 | IPIQASLPF | IPIQASLLF | 425 | 1465 |
| HLA-B*15:03 | ATIPIQASLPF | ATIPIQASLLF | 307 | 1003 |
| HLA-B*15:01 | RATATIPIQASLPF | RATATIPIQASLLF | 429 | 1366 |
| HLA-A*02:01 | IQASLPFGWLIV | IQASLLFGWLIV | 1078 | 359 |
| HLA-A*02:11 | SLPFGWLIV | SLLFGWLIV | 21 | 7 |
| HLA-A*02:11 | IQASLPFGWLIV | IQASLLFGWLIV | 544 | 186 |
| HLA-A*02:11 | SLPFGWLIVGVA | SLLFGWLIVGVA | 663 | 228 |
| HLA-B*15:03 | RATATIPIQASLPF | RATATIPIQASLLF | 159 | 454 |
| HLA-B*58:01 | ATIPIQASLPF | ATIPIQASLLF | 250 | 91 |
| HLA-A*02:06 | SLPFGWLIV | SLLFGWLIV | 200 | 74 |
| HLA-C*03:02 | IPIQASLPF | IPIQASLLF | 140 | 373 |
| HLA-A*02:01 | IQASLPFGWL | IQASLLFGWL | 550 | 208 |
| HLA-A*02:11 | IQASLPFGWL | IQASLLFGWL | 342 | 132 |
| HLA-A*02:11 | ASLPFGWLIVGV | ASLLFGWLIVGV | 276 | 111 |
| HLA-B*15:03 | IPIQASLPF | IPIQASLLF | 39 | 93 |
| HLA-A*02:06 | IQASLPFGWLIVGV | IQASLLFGWLIVGV | 387 | 163 |
| HLA-A*02:11 | SLPFGWLIVGV | SLLFGWLIVGV | 26 | 11 |
| HLA-B*53:01 | IPIQASLPFG | IPIQASLLFG | 1146 | 491 |
| HLA-B*53:01 | TIPIQASLPF | TIPIQASLLF | 218 | 98 |
| HLA-B*53:01 | TATIPIQASLPF | TATIPIQASLLF | 937 | 423 |
| HLA-B*57:01 | ATIPIQASLPF | ATIPIQASLLF | 369 | 169 |
| HLA-B*35:01 | IPIQASLPF | IPIQASLLF | 3 | 6 |
Q57H
Reference TATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCN
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TATIPIQASLLFGWLIVGVALLAVFHSASKIITLKKRWQLALSKGVHFVCN
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-B*15:01 | FQSASKIITL | FHSASKIITL | 156 | 6196 |
| HLA-A*02:06 | FQSASKIITL | FHSASKIITL | 43 | 1441 |
| HLA-A*02:01 | FQSASKIITL | FHSASKIITL | 205 | 5629 |
| HLA-A*02:11 | FQSASKIITL | FHSASKIITL | 79 | 2010 |
| HLA-A*02:02 | FQSASKIITL | FHSASKIITL | 38 | 957 |
| HLA-B*38:01 | FQSASKIITL | FHSASKIITL | 2071 | 94 |
| HLA-B*15:03 | FQSASKIITL | FHSASKIITL | 88 | 851 |
| HLA-C*03:03 | QSASKIITL | HSASKIITL | 285 | 37 |
| HLA-C*03:04 | QSASKIITL | HSASKIITL | 285 | 37 |
| HLA-C*03:02 | QSASKIITL | HSASKIITL | 724 | 94 |
| HLA-C*12:03 | QSASKIITL | HSASKIITL | 250 | 34 |
| HLA-C*12:02 | QSASKIITL | HSASKIITL | 1730 | 284 |
| HLA-C*02:02 | QSASKIITL | HSASKIITL | 2653 | 440 |
| HLA-C*02:10 | QSASKIITL | HSASKIITL | 2653 | 440 |
| HLA-A*68:02 | QSASKIITL | HSASKIITL | 1085 | 232 |
| HLA-C*17:01 | QSASKIITL | HSASKIITL | 453 | 102 |
| HLA-A*30:01 | QSASKIITLK | HSASKIITLK | 727 | 164 |
| HLA-A*30:01 | LAVFQSASK | LAVFHSASK | 1169 | 264 |
| HLA-C*15:02 | QSASKIITL | HSASKIITL | 222 | 51 |
| HLA-C*16:01 | QSASKIITL | HSASKIITL | 52 | 12 |
| HLA-A*68:01 | QSASKIITLKK | HSASKIITLKK | 1356 | 322 |
| HLA-A*30:01 | QSASKIITLKK | HSASKIITLKK | 1813 | 486 |
| HLA-A*68:01 | QSASKIITLKKR | HSASKIITLKKR | 683 | 195 |
| HLA-A*68:01 | QSASKIITLK | HSASKIITLK | 35 | 10 |
| HLA-A*02:11 | LLAVFQSA | LLAVFHSA | 1121 | 356 |
| HLA-A*31:01 | QSASKIITLKKR | HSASKIITLKKR | 968 | 344 |
| HLA-A*03:01 | QSASKIITLK | HSASKIITLK | 214 | 83 |
| HLA-A*03:01 | AVFQSASKIITLKK | AVFHSASKIITLKK | 227 | 90 |
| HLA-A*02:02 | LLAVFQSASKII | LLAVFHSASKII | 298 | 727 |
| HLA-A*03:01 | AVFQSASK | AVFHSASK | 795 | 335 |
| HLA-A*03:01 | AVFQSASKIITLK | AVFHSASKIITLK | 74 | 32 |
| HLA-A*03:02 | AVFQSASKIITLKK | AVFHSASKIITLKK | 619 | 272 |
| HLA-A*02:11 | GVALLAVFQSA | GVALLAVFHSA | 895 | 401 |
| HLA-A*68:01 | AVFQSASKIITLK | AVFHSASKIITLK | 808 | 368 |
| HLA-A*03:02 | AVFQSASKIITLK | AVFHSASKIITLK | 170 | 82 |
| HLA-A*02:11 | VALLAVFQSA | VALLAVFHSA | 173 | 85 |
| HLA-A*02:11 | ALLAVFQSAS | ALLAVFHSAS | 691 | 340 |
| HLA-A*02:01 | ALLAVFQSA | ALLAVFHSA | 42 | 21 |
| HLA-B*58:01 | QSASKIITLKKRW | HSASKIITLKKRW | 142 | 71 |
NS8 protein
T11I
Reference MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDP
|||||||||| |||||||||||||||||||||||||
Mutated MKFLVFLGIIITVAAFHQECSLQSCTQHQPYVVDDP
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | TTVAAFHQECSL | ITVAAFHQECSL | 288 | 2832 |
| HLA-A*02:11 | FLVFLGIIT | FLVFLGIII | 1125 | 153 |
| HLA-A*02:01 | FLVFLGIITT | FLVFLGIIIT | 83 | 564 |
| HLA-B*15:01 | FLGIITTVAAF | FLGIIITVAAF | 131 | 835 |
| HLA-A*02:06 | FLVFLGIITT | FLVFLGIIIT | 234 | 1444 |
| HLA-A*02:11 | FLVFLGIITT | FLVFLGIIIT | 45 | 273 |
| HLA-A*02:06 | LVFLGIITT | LVFLGIIIT | 366 | 2114 |
| HLA-A*02:02 | FLVFLGIITT | FLVFLGIIIT | 83 | 468 |
| HLA-C*14:02 | VFLGIITTV | VFLGIIITV | 189 | 1058 |
| HLA-A*02:02 | KFLVFLGIITTV | KFLVFLGIIITV | 215 | 1039 |
| HLA-A*02:02 | FLVFLGIITTV | FLVFLGIIITV | 57 | 257 |
| HLA-B*15:01 | VFLGIITTVAAF | VFLGIIITVAAF | 439 | 1864 |
| HLA-B*15:03 | FLGIITTVAAF | FLGIIITVAAF | 484 | 2052 |
| HLA-B*15:01 | LGIITTVAAF | LGIIITVAAF | 18 | 73 |
| HLA-B*15:01 | FLVFLGIITTVAAF | FLVFLGIIITVAAF | 485 | 1933 |
| HLA-B*15:01 | LVFLGIITTVAAF | LVFLGIIITVAAF | 219 | 851 |
| HLA-A*02:06 | KFLVFLGIITTV | KFLVFLGIIITV | 382 | 1347 |
| HLA-C*03:02 | GIITTVAAF | GIIITVAAF | 338 | 1191 |
| HLA-A*02:01 | KFLVFLGIITTV | KFLVFLGIIITV | 138 | 436 |
| HLA-B*15:03 | GIITTVAAF | GIIITVAAF | 40 | 123 |
| HLA-A*02:06 | FLVFLGIITTV | FLVFLGIIITV | 64 | 189 |
| HLA-B*15:03 | LGIITTVAAF | LGIIITVAAF | 123 | 360 |
| HLA-A*02:11 | KFLVFLGIITTV | KFLVFLGIIITV | 36 | 101 |
| HLA-A*02:02 | FLVFLGIITTVA | FLVFLGIIITVA | 263 | 730 |
| HLA-A*02:01 | FLVFLGIITTVA | FLVFLGIIITVA | 184 | 506 |
| HLA-B*15:01 | GIITTVAAF | GIIITVAAF | 18 | 48 |
| HLA-A*02:11 | FLVFLGIITTVA | FLVFLGIIITVA | 56 | 147 |
| HLA-A*02:01 | FLVFLGIITTV | FLVFLGIIITV | 25 | 64 |
| HLA-A*02:11 | MKFLVFLGIITTV | MKFLVFLGIIITV | 205 | 516 |
| HLA-A*02:02 | LVFLGIITTV | LVFLGIIITV | 94 | 217 |
| HLA-A*02:02 | FLGIITTV | FLGIIITV | 29 | 66 |
| HLA-A*02:11 | FLVFLGIITTVAA | FLVFLGIIITVAA | 248 | 557 |
| HLA-A*02:06 | FLGIITTVAA | FLGIIITVAA | 282 | 603 |
| HLA-A*02:11 | FLVFLGIITTV | FLVFLGIIITV | 7 | 14 |
A51S
Reference TQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDI
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated TQHQPYVVDDPCPIHFYSKWYIRVGSRKSAPLIELCVDEAGSKSPIQYIDI
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | YSKWYIRVGA | YSKWYIRVGS | 262 | 3511 |
| HLA-A*30:01 | YSKWYIRVGA | YSKWYIRVGS | 229 | 894 |
| HLA-A*30:01 | GARKSAPLI | GSRKSAPLI | 55 | 19 |
| HLA-A*30:01 | VGARKSAPLI | VGSRKSAPLI | 599 | 249 |
NS9c protein
L52F
Reference VKPLLVPHHVVATVQEIQLQAAVGELLLLEWLAMAVMLLLLCCCLTD
||||||||||||||||||||||||| |||||||||||||||||||||
Mutated VKPLLVPHHVVATVQEIQLQAAVGEFLLLEWLAMAVMLLLLCCCLTD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | QLQAAVGEL | QLQAAVGEF | 120 | 5031 |
| HLA-A*02:11 | GELLLLEWLA | GEFLLLEWLA | 267 | 9767 |
| HLA-B*15:01 | QLQAAVGEL | QLQAAVGEF | 2048 | 65 |
| HLA-A*02:01 | GELLLLEWLA | GEFLLLEWLA | 409 | 12481 |
| HLA-A*02:02 | QLQAAVGEL | QLQAAVGEF | 92 | 2789 |
| HLA-B*15:03 | QLQAAVGEL | QLQAAVGEF | 2231 | 81 |
| HLA-A*02:02 | IQLQAAVGEL | IQLQAAVGEF | 170 | 4413 |
| HLA-A*02:11 | ELLLLEWLA | EFLLLEWLA | 345 | 8719 |
| HLA-A*02:11 | IQLQAAVGEL | IQLQAAVGEF | 338 | 7506 |
| HLA-B*15:01 | IQLQAAVGEL | IQLQAAVGEF | 467 | 24 |
| HLA-B*15:03 | IQLQAAVGEL | IQLQAAVGEF | 168 | 10 |
| HLA-B*15:03 | LQAAVGEL | LQAAVGEF | 295 | 18 |
| HLA-B*15:03 | VQEIQLQAAVGEL | VQEIQLQAAVGEF | 6575 | 430 |
| HLA-A*02:11 | LLLLEWLAM | FLLLEWLAM | 283 | 20 |
| HLA-B*40:01 | QEIQLQAAVGEL | QEIQLQAAVGEF | 201 | 2708 |
| HLA-B*15:01 | LQAAVGEL | LQAAVGEF | 1712 | 138 |
| HLA-A*02:01 | LLLLEWLAM | FLLLEWLAM | 573 | 54 |
| HLA-A*02:11 | LLLLEWLA | FLLLEWLA | 844 | 82 |
| HLA-A*02:02 | LLLLEWLAM | FLLLEWLAM | 1379 | 150 |
| HLA-B*44:03 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1386 | 164 |
| HLA-A*02:06 | LLLLEWLAM | FLLLEWLAM | 763 | 94 |
| HLA-A*02:01 | LLLLEWLA | FLLLEWLA | 1287 | 163 |
| HLA-B*44:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 1403 | 179 |
| HLA-A*02:02 | LLLLEWLA | FLLLEWLA | 2866 | 389 |
| HLA-C*03:02 | LLLLEWLAM | FLLLEWLAM | 2566 | 358 |
| HLA-A*02:06 | IQLQAAVGEL | IQLQAAVGEF | 280 | 1893 |
| HLA-A*02:06 | LLLLEWLA | FLLLEWLA | 2803 | 489 |
| HLA-A*02:02 | LLLLEWLAMA | FLLLEWLAMA | 67 | 13 |
| HLA-A*02:01 | ELLLLEWLAMA | EFLLLEWLAMA | 1276 | 266 |
| HLA-A*02:11 | QLQAAVGELLL | QLQAAVGEFLL | 733 | 157 |
| HLA-B*40:02 | QEIQLQAAVGEL | QEIQLQAAVGEF | 334 | 1488 |
| HLA-A*02:06 | LLLLEWLAMA | FLLLEWLAMA | 49 | 11 |
| HLA-A*02:11 | LLLLEWLAMAVM | FLLLEWLAMAVM | 926 | 212 |
| HLA-A*02:02 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 714 | 164 |
| HLA-A*02:01 | LLLLEWLAMAVML | FLLLEWLAMAVML | 1353 | 320 |
| HLA-A*02:11 | LLLLEWLAMAVML | FLLLEWLAMAVML | 385 | 97 |
| HLA-A*02:11 | LQAAVGELLL | LQAAVGEFLL | 1331 | 346 |
| HLA-A*02:01 | LLLLEWLAMA | FLLLEWLAMA | 23 | 6 |
| HLA-A*02:11 | ELLLLEWLAMA | EFLLLEWLAMA | 299 | 79 |
| HLA-A*02:11 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 544 | 146 |
| HLA-A*02:01 | LLLLEWLAMAVMLL | FLLLEWLAMAVMLL | 1361 | 373 |
| HLA-B*40:01 | GELLLLEWLAM | GEFLLLEWLAM | 791 | 222 |
| HLA-B*15:03 | AAVGELLLL | AAVGEFLLL | 1475 | 426 |
| HLA-A*02:01 | QLQAAVGELLL | QLQAAVGEFLL | 1401 | 441 |
| HLA-A*02:02 | LLLLEWLAMAV | FLLLEWLAMAV | 453 | 147 |
| HLA-C*03:03 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-C*03:04 | QAAVGELLL | QAAVGEFLL | 283 | 829 |
| HLA-A*02:02 | QLQAAVGELLL | QLQAAVGEFLL | 530 | 186 |
| HLA-B*50:01 | GELLLLEWLA | GEFLLLEWLA | 1298 | 484 |
| HLA-A*02:11 | LLLLEWLAMA | FLLLEWLAMA | 8 | 3 |
| HLA-A*02:01 | LQAAVGELLL | LQAAVGEFLL | 917 | 347 |
| HLA-C*03:03 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-C*03:04 | AAVGELLLL | AAVGEFLLL | 107 | 41 |
| HLA-A*02:06 | LQAAVGELLL | LQAAVGEFLL | 162 | 63 |
| HLA-B*40:06 | GELLLLEWLA | GEFLLLEWLA | 268 | 106 |
| HLA-B*40:02 | GELLLLEWLAM | GEFLLLEWLAM | 201 | 83 |
| HLA-A*02:02 | LQAAVGELLL | LQAAVGEFLL | 100 | 42 |
| HLA-C*03:02 | AAVGELLLL | AAVGEFLLL | 269 | 116 |
| HLA-A*02:01 | LLLLEWLAMAV | FLLLEWLAMAV | 97 | 44 |
| HLA-A*02:11 | QLQAAVGELL | QLQAAVGEFL | 303 | 139 |
| HLA-B*15:01 | LQAAVGELLL | LQAAVGEFLL | 109 | 237 |
| HLA-B*15:03 | LQAAVGELLL | LQAAVGEFLL | 98 | 210 |
| HLA-A*02:11 | LQAAVGELL | LQAAVGEFL | 386 | 183 |
| HLA-B*40:02 | GELLLLEWLAMAV | GEFLLLEWLAMAV | 815 | 391 |
| HLA-B*40:02 | GELLLLEWLA | GEFLLLEWLA | 475 | 231 |
| HLA-A*02:06 | IQLQAAVGELLL | IQLQAAVGEFLL | 626 | 310 |
| HLA-C*16:01 | AAVGELLLL | AAVGEFLLL | 205 | 102 |
NSP2 protein
T85I
Reference WYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEK
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Mutated WYTERSEKSYELQTPFEIKLAKKFDIFNGECPNFVFPLNSIIKTIQPRVEK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*68:02 | DTFNGECPNFVFPL | DIFNGECPNFVFPL | 203 | 1683 |
| HLA-A*68:02 | DTFNGECPNFV | DIFNGECPNFV | 60 | 311 |
| HLA-A*32:01 | KLAKKFDTF | KLAKKFDIF | 44 | 159 |
| HLA-A*24:02 | KLAKKFDTF | KLAKKFDIF | 237 | 578 |
| HLA-B*15:03 | KKFDTFNGECPNF | KKFDIFNGECPNF | 226 | 542 |
| HLA-A*23:01 | TFNGECPNF | IFNGECPNF | 517 | 219 |
| HLA-A*23:01 | TFNGECPNFVF | IFNGECPNFVF | 425 | 191 |
| HLA-A*23:01 | KLAKKFDTF | KLAKKFDIF | 284 | 624 |
NSP4 protein
K399E
Reference PFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNK
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated PFWITIAYIICISTKHFYWFFSNYLERRVVFNGVSFSTFEEAALCTFLLNK
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*11:01 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 23 | 19000 |
| HLA-A*03:02 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 99 | 19924 |
| HLA-A*68:01 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 44 | 7593 |
| HLA-A*31:01 | FYWFFSNYLK | FYWFFSNYLE | 36 | 3176 |
| HLA-A*31:01 | HFYWFFSNYLK | HFYWFFSNYLE | 59 | 5103 |
| HLA-A*68:01 | FYWFFSNYLK | FYWFFSNYLE | 38 | 3279 |
| HLA-A*11:01 | YWFFSNYLK | YWFFSNYLE | 183 | 14582 |
| HLA-A*68:01 | HFYWFFSNYLK | HFYWFFSNYLE | 108 | 8458 |
| HLA-A*31:01 | YWFFSNYLK | YWFFSNYLE | 40 | 2756 |
| HLA-A*03:01 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 312 | 21449 |
| HLA-A*11:01 | FYWFFSNYLK | FYWFFSNYLE | 290 | 19830 |
| HLA-A*03:02 | FYWFFSNYLK | FYWFFSNYLE | 184 | 12494 |
| HLA-A*03:02 | YWFFSNYLK | YWFFSNYLE | 150 | 9439 |
| HLA-A*03:02 | HFYWFFSNYLK | HFYWFFSNYLE | 259 | 15089 |
| HLA-A*68:01 | YWFFSNYLK | YWFFSNYLE | 50 | 2894 |
| HLA-A*11:01 | HFYWFFSNYLK | HFYWFFSNYLE | 455 | 24513 |
| HLA-A*31:01 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 169 | 8198 |
| HLA-A*33:03 | FYWFFSNYLK | FYWFFSNYLE | 45 | 2158 |
| HLA-B*08:01 | YLKRRVVFNGVSF | YLERRVVFNGVSF | 53 | 2200 |
| HLA-A*33:03 | HFYWFFSNYLK | HFYWFFSNYLE | 105 | 4130 |
| HLA-A*31:01 | KHFYWFFSNYLK | KHFYWFFSNYLE | 101 | 3884 |
| HLA-A*33:03 | YWFFSNYLK | YWFFSNYLE | 53 | 1783 |
| HLA-B*08:01 | SNYLKRRVV | SNYLERRVV | 131 | 3344 |
| HLA-B*08:01 | YLKRRVVFNGV | YLERRVVFNGV | 218 | 5504 |
| HLA-A*30:01 | YWFFSNYLK | YWFFSNYLE | 318 | 7629 |
| HLA-A*30:01 | HFYWFFSNYLK | HFYWFFSNYLE | 463 | 10847 |
| HLA-A*30:01 | STKHFYWFFSNYLK | STKHFYWFFSNYLE | 356 | 7660 |
| HLA-B*27:05 | KRRVVFNGV | ERRVVFNGV | 284 | 4785 |
| HLA-B*08:01 | YLKRRVVF | YLERRVVF | 10 | 122 |
| HLA-A*30:01 | KRRVVFNGV | ERRVVFNGV | 486 | 5351 |
| HLA-A*29:02 | FYWFFSNYLK | FYWFFSNYLE | 292 | 2013 |
| HLA-A*29:02 | YWFFSNYLK | YWFFSNYLE | 219 | 1202 |
| HLA-A*68:01 | FFSNYLKRR | FFSNYLERR | 1541 | 310 |
| HLA-B*15:01 | YLKRRVVFNGVSF | YLERRVVFNGVSF | 210 | 1013 |
| HLA-B*15:03 | LKRRVVFNGVSF | LERRVVFNGVSF | 74 | 324 |
| HLA-B*15:03 | KRRVVFNGVSF | ERRVVFNGVSF | 328 | 1183 |
| HLA-C*14:02 | SNYLKRRVVF | SNYLERRVVF | 202 | 61 |
| HLA-A*68:02 | FSNYLKRRV | FSNYLERRV | 1253 | 416 |
| HLA-C*14:02 | FSNYLKRRVVF | FSNYLERRVVF | 434 | 147 |
| HLA-B*27:05 | KRRVVFNGVSF | ERRVVFNGVSF | 387 | 1030 |
| HLA-C*07:02 | NYLKRRVVF | NYLERRVVF | 167 | 66 |
| HLA-A*02:11 | YLKRRVVFNGV | YLERRVVFNGV | 194 | 82 |
| HLA-C*14:02 | YWFFSNYLKRRVVF | YWFFSNYLERRVVF | 575 | 264 |
| HLA-A*68:01 | WFFSNYLKRR | WFFSNYLERR | 315 | 155 |
L438P
Reference EEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSY
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated EEAALCTFLLNKEMYLKLRSDVLLPPTQYNRYLVLYNKYKYFSGAMDTTSY
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:11 | KLRSDVLLPL | KLRSDVLLPP | 5 | 1146 |
| HLA-C*06:02 | LRSDVLLPL | LRSDVLLPP | 103 | 17926 |
| HLA-A*02:11 | YLKLRSDVLLPL | YLKLRSDVLLPP | 5 | 788 |
| HLA-A*02:11 | LKLRSDVLLPL | LKLRSDVLLPP | 93 | 14621 |
| HLA-C*07:02 | LRSDVLLPL | LRSDVLLPP | 104 | 15467 |
| HLA-C*07:01 | LRSDVLLPL | LRSDVLLPP | 104 | 15210 |
| HLA-A*02:01 | KLRSDVLLPL | KLRSDVLLPP | 10 | 1434 |
| HLA-A*02:02 | KLRSDVLLPL | KLRSDVLLPP | 6 | 686 |
| HLA-A*02:11 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 82 | 9276 |
| HLA-A*02:02 | LKLRSDVLLPL | LKLRSDVLLPP | 120 | 12620 |
| HLA-A*02:01 | LKLRSDVLLPL | LKLRSDVLLPP | 161 | 15657 |
| HLA-A*02:02 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 49 | 4677 |
| HLA-A*02:02 | LPLTQYNRYL | LPPTQYNRYL | 241 | 20700 |
| HLA-B*08:01 | YLKLRSDVLLPL | YLKLRSDVLLPP | 13 | 1103 |
| HLA-A*02:01 | YLKLRSDVLLPL | YLKLRSDVLLPP | 11 | 889 |
| HLA-A*02:02 | YLKLRSDVLLPL | YLKLRSDVLLPP | 6 | 417 |
| HLA-A*02:06 | KLRSDVLLPL | KLRSDVLLPP | 29 | 1825 |
| HLA-A*02:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 161 | 9143 |
| HLA-C*14:02 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 313 | 17335 |
| HLA-C*08:02 | RSDVLLPL | RSDVLLPP | 400 | 21753 |
| HLA-B*27:05 | LRSDVLLPL | LRSDVLLPP | 81 | 4120 |
| HLA-B*15:03 | LRSDVLLPL | LRSDVLLPP | 149 | 6800 |
| HLA-B*27:05 | KLRSDVLLPL | KLRSDVLLPP | 94 | 4082 |
| HLA-A*02:06 | YLKLRSDVLLPL | YLKLRSDVLLPP | 35 | 1507 |
| HLA-A*02:01 | KLRSDVLLPLT | KLRSDVLLPPT | 144 | 5473 |
| HLA-A*02:02 | KLRSDVLLPLT | KLRSDVLLPPT | 183 | 6897 |
| HLA-B*08:01 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 231 | 8566 |
| HLA-B*38:01 | LRSDVLLPL | LRSDVLLPP | 499 | 18496 |
| HLA-A*02:11 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 400 | 14119 |
| HLA-B*08:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 151 | 5303 |
| HLA-B*27:05 | YLKLRSDVLLPL | YLKLRSDVLLPP | 270 | 9416 |
| HLA-A*32:01 | KLRSDVLLPL | KLRSDVLLPP | 418 | 14267 |
| HLA-A*30:01 | LTQYNRYLA | PTQYNRYLV | 171 | 5507 |
| HLA-A*02:02 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 82 | 2621 |
| HLA-C*16:01 | LTQYNRYLAL | PTQYNRYLVL | 285 | 9019 |
| HLA-A*02:02 | EMYLKLRSDVLLPL | EMYLKLRSDVLLPP | 322 | 9290 |
| HLA-B*27:05 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 378 | 10377 |
| HLA-A*02:11 | LLPLTQYNRYLA | LLPPTQYNRYLV | 1450 | 57 |
| HLA-B*15:01 | LTQYNRYLAL | PTQYNRYLVL | 125 | 3068 |
| HLA-A*02:11 | KLRSDVLLPLT | KLRSDVLLPPT | 114 | 2763 |
| HLA-A*23:01 | MYLKLRSDVLLPL | MYLKLRSDVLLPP | 466 | 10083 |
| HLA-B*15:01 | KLRSDVLLPL | KLRSDVLLPP | 335 | 6450 |
| HLA-A*02:06 | LTQYNRYLAL | PTQYNRYLVL | 483 | 7006 |
| HLA-B*15:03 | LTQYNRYLAL | PTQYNRYLVL | 15 | 197 |
| HLA-A*02:01 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 177 | 2183 |
| HLA-A*02:11 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 229 | 19 |
| HLA-B*08:01 | LTQYNRYLAL | PTQYNRYLVL | 126 | 1517 |
| HLA-A*68:02 | LTQYNRYLA | PTQYNRYLV | 349 | 3473 |
| HLA-A*11:01 | LTQYNRYLALYNK | PTQYNRYLVLYNK | 388 | 3729 |
| HLA-B*53:01 | LPLTQYNRY | LPPTQYNRY | 497 | 4773 |
| HLA-A*02:11 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 115 | 1005 |
| HLA-B*35:01 | LPLTQYNRY | LPPTQYNRY | 66 | 540 |
| HLA-A*02:02 | LLPLTQYNRYLA | LLPPTQYNRYLV | 1272 | 178 |
| HLA-B*08:01 | YLKLRSDVLLPLT | YLKLRSDVLLPPT | 258 | 1470 |
| HLA-A*01:01 | LTQYNRYLALY | PTQYNRYLVLY | 400 | 1908 |
| HLA-A*02:01 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 545 | 117 |
| HLA-A*02:06 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 1552 | 412 |
| HLA-A*02:11 | LLPLTQYNRYL | LLPPTQYNRYL | 389 | 105 |
| HLA-A*30:01 | KLRSDVLLPL | KLRSDVLLPP | 108 | 390 |
| HLA-A*30:02 | LTQYNRYLALY | PTQYNRYLVLY | 92 | 330 |
| HLA-A*02:02 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 980 | 284 |
| HLA-B*07:02 | LPLTQYNRYLAL | LPPTQYNRYLVL | 100 | 333 |
| HLA-A*02:01 | VLLPLTQYNRYLAL | VLLPPTQYNRYLVL | 343 | 1088 |
| HLA-A*29:02 | LTQYNRYLALY | PTQYNRYLVLY | 212 | 636 |
| HLA-B*08:01 | LPLTQYNRYLAL | LPPTQYNRYLVL | 58 | 163 |
| HLA-A*02:02 | LLPLTQYNRYL | LLPPTQYNRYL | 400 | 145 |
| HLA-B*08:01 | LLPLTQYNRYLAL | LLPPTQYNRYLVL | 190 | 517 |
| HLA-A*02:01 | VLLPLTQYNRYL | VLLPPTQYNRYL | 170 | 445 |
| HLA-B*42:01 | LPLTQYNRYLAL | LPPTQYNRYLVL | 29 | 74 |
| HLA-A*02:02 | VLLPLTQYNRYLAL | VLLPPTQYNRYLVL | 381 | 940 |
| HLA-A*02:02 | VLLPLTQYNRYL | VLLPPTQYNRYL | 147 | 335 |
| HLA-B*42:01 | LPLTQYNRYL | LPPTQYNRYL | 72 | 154 |
| HLA-A*29:02 | VLLPLTQYNRY | VLLPPTQYNRY | 143 | 302 |
| HLA-A*30:02 | PLTQYNRYLALY | PPTQYNRYLVLY | 379 | 778 |
A446V
Reference LLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHL
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated LLNKEMYLKLRSDVLLPPTQYNRYLVLYNKYKYFSGAMDTTSYREAACCHL
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*30:01 | LTQYNRYLA | PTQYNRYLV | 171 | 5507 |
| HLA-C*16:01 | LTQYNRYLAL | PTQYNRYLVL | 285 | 9019 |
| HLA-A*02:11 | LLPLTQYNRYLA | LLPPTQYNRYLV | 1450 | 57 |
| HLA-B*15:01 | LTQYNRYLAL | PTQYNRYLVL | 125 | 3068 |
| HLA-C*16:01 | LALYNKYKY | LVLYNKYKY | 76 | 1859 |
| HLA-A*02:06 | LTQYNRYLAL | PTQYNRYLVL | 483 | 7006 |
| HLA-C*16:01 | LALYNKYKYF | LVLYNKYKYF | 347 | 4564 |
| HLA-B*15:03 | LTQYNRYLAL | PTQYNRYLVL | 15 | 197 |
| HLA-A*02:11 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 229 | 19 |
| HLA-B*08:01 | LTQYNRYLAL | PTQYNRYLVL | 126 | 1517 |
| HLA-A*68:02 | LTQYNRYLA | PTQYNRYLV | 349 | 3473 |
| HLA-C*03:02 | LALYNKYKY | LVLYNKYKY | 124 | 1202 |
| HLA-A*11:01 | LTQYNRYLALYNK | PTQYNRYLVLYNK | 388 | 3729 |
| HLA-C*12:03 | LALYNKYKY | LVLYNKYKY | 461 | 4287 |
| HLA-B*35:01 | LALYNKYKY | LVLYNKYKY | 142 | 1049 |
| HLA-A*02:02 | LLPLTQYNRYLA | LLPPTQYNRYLV | 1272 | 178 |
| HLA-B*58:01 | LALYNKYKYF | LVLYNKYKYF | 280 | 1881 |
| HLA-C*03:02 | LALYNKYKYF | LVLYNKYKYF | 356 | 2340 |
| HLA-B*58:01 | LALYNKYKY | LVLYNKYKY | 474 | 2939 |
| HLA-A*01:01 | LTQYNRYLALY | PTQYNRYLVLY | 400 | 1908 |
| HLA-C*03:02 | YLALYNKYKY | YLVLYNKYKY | 500 | 2355 |
| HLA-A*02:01 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 545 | 117 |
| HLA-A*29:02 | LALYNKYKY | LVLYNKYKY | 146 | 35 |
| HLA-A*23:01 | ALYNKYKYF | VLYNKYKYF | 963 | 250 |
| HLA-A*02:06 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 1552 | 412 |
| HLA-A*30:02 | LTQYNRYLALY | PTQYNRYLVLY | 92 | 330 |
| HLA-A*02:02 | VLLPLTQYNRYLA | VLLPPTQYNRYLV | 980 | 284 |
| HLA-B*07:02 | LPLTQYNRYLAL | LPPTQYNRYLVL | 100 | 333 |
| HLA-A*03:02 | YLALYNKYK | YLVLYNKYK | 47 | 151 |
| HLA-A*02:01 | VLLPLTQYNRYLAL | VLLPPTQYNRYLVL | 343 | 1088 |
| HLA-A*24:02 | RYLALYNKYKYF | RYLVLYNKYKYF | 49 | 151 |
| HLA-B*15:03 | LALYNKYKY | LVLYNKYKY | 250 | 768 |
| HLA-A*29:02 | LTQYNRYLALY | PTQYNRYLVLY | 212 | 636 |
| HLA-A*03:01 | YLALYNKYK | YLVLYNKYK | 102 | 305 |
| HLA-C*14:02 | RYLALYNKY | RYLVLYNKY | 82 | 235 |
| HLA-A*23:01 | RYLALYNKYKYF | RYLVLYNKYKYF | 21 | 60 |
| HLA-B*08:01 | LPLTQYNRYLAL | LPPTQYNRYLVL | 58 | 163 |
| HLA-A*02:11 | TQYNRYLAL | TQYNRYLVL | 144 | 395 |
| HLA-B*08:01 | LLPLTQYNRYLAL | LLPPTQYNRYLVL | 190 | 517 |
| HLA-A*29:02 | YNRYLALYNKY | YNRYLVLYNKY | 226 | 604 |
| HLA-A*03:02 | RYLALYNKYK | RYLVLYNKYK | 35 | 91 |
| HLA-B*42:01 | TQYNRYLAL | TQYNRYLVL | 478 | 1233 |
| HLA-B*42:01 | LPLTQYNRYLAL | LPPTQYNRYLVL | 29 | 74 |
| HLA-A*02:06 | TQYNRYLAL | TQYNRYLVL | 57 | 144 |
| HLA-A*02:02 | YLALYNKYKYFSGA | YLVLYNKYKYFSGA | 247 | 614 |
| HLA-A*02:02 | VLLPLTQYNRYLAL | VLLPPTQYNRYLVL | 381 | 940 |
| HLA-A*29:02 | YLALYNKY | YLVLYNKY | 383 | 916 |
| HLA-A*02:02 | TQYNRYLAL | TQYNRYLVL | 349 | 828 |
| HLA-A*11:01 | YLALYNKYK | YLVLYNKYK | 197 | 445 |
| HLA-A*30:02 | QYNRYLALY | QYNRYLVLY | 109 | 246 |
| HLA-A*03:01 | RYLALYNKYK | RYLVLYNKYK | 27 | 58 |
| HLA-A*24:02 | RYLALYNKY | RYLVLYNKY | 333 | 709 |
| HLA-A*29:02 | RYLALYNKY | RYLVLYNKY | 219 | 465 |
| HLA-A*30:02 | PLTQYNRYLALY | PPTQYNRYLVLY | 379 | 778 |
| HLA-A*23:01 | RYLALYNKY | RYLVLYNKY | 156 | 314 |
| HLA-B*08:01 | TQYNRYLAL | TQYNRYLVL | 33 | 66 |
NSP6 protein
SGF 106-108 deletion
Reference FNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYD
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated FNMVYMPASWVMRIMTWLDMVDTSL‑‑‑KLKDCVMYASAVVLLILMTARTVYD
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:02 | FKLKDCVMYA | - | 8 | - |
| HLA-A*02:11 | FKLKDCVMYA | - | 12 | - |
| HLA-A*68:01 | MVDTSLSGFK | MVDTSLK | 13 | - |
| HLA-B*15:03 | FKLKDCVMY | - | 15 | - |
| HLA-A*11:01 | MVDTSLSGFK | MVDTSLK | 21 | - |
| HLA-A*11:01 | TSLSGFKLK | TSLKLK | 22 | - |
| HLA-A*03:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 25 |
| HLA-A*02:01 | FKLKDCVMYA | - | 27 | - |
| HLA-A*68:01 | DTSLSGFKLK | DTSLKLK | 33 | - |
| HLA-A*03:02 | MVDTSLSGFK | MVDTSLK | 35 | - |
| HLA-A*68:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 37 |
| HLA-A*03:01 | MVDTSLSGFK | MVDTSLK | 54 | - |
| HLA-A*03:02 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 59 |
| HLA-C*05:01 | MVDTSLSGF | MVDTSL | 64 | - |
| HLA-A*11:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 71 |
| HLA-A*02:06 | FKLKDCVMYA | - | 90 | - |
| HLA-A*30:01 | TSLSGFKLK | TSLKLK | 107 | - |
| HLA-A*02:02 | SGFKLKDCVMYA | - | 126 | - |
| HLA-A*11:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 128 |
| HLA-A*03:02 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 147 |
| HLA-C*08:02 | MVDTSLSGF | MVDTSL | 159 | - |
| HLA-A*03:02 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 168 |
| HLA-A*02:11 | SGFKLKDCVMYA | - | 200 | - |
| HLA-A*02:02 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 202 |
| HLA-A*01:01 | MVDTSLSGFKLKDCVMY | MVDTSLKLKDCVMY | - | 207 |
| HLA-C*03:02 | MVDTSLSGF | MVDTSL | 211 | - |
| HLA-A*02:02 | GFKLKDCVMYA | - | 239 | - |
| HLA-A*02:02 | TSLSGFKLKDCVMYA | TSLKLKDCVMYA | - | 244 |
| HLA-A*03:01 | TSLSGFKLK | TSLKLK | 244 | - |
| HLA-B*15:03 | MVDTSLSGF | MVDTSL | 276 | - |
| HLA-A*68:01 | TSLSGFKLK | TSLKLK | 286 | - |
| HLA-A*03:02 | TSLSGFKLK | TSLKLK | 289 | - |
| HLA-A*03:01 | IMTWLDMVDTSLSGFK | IMTWLDMVDTSLK | - | 309 |
| HLA-A*68:02 | DTSLSGFKL | DTSLKL | 313 | - |
| HLA-A*02:11 | MTWLDMVDTSLSGFKL | MTWLDMVDTSLKL | - | 346 |
| HLA-B*15:01 | DMVDTSLSGF | DMVDTSL | 362 | - |
| HLA-A*23:01 | TWLDMVDTSLSGF | - | 387 | - |
| HLA-A*02:11 | GFKLKDCVMYA | - | 394 | - |
| HLA-A*03:01 | MTWLDMVDTSLSGFK | MTWLDMVDTSLK | - | 415 |
| HLA-A*26:01 | DMVDTSLSGF | DMVDTSL | 417 | - |
| HLA-B*08:01 | MVDTSLSGFKLKDCVM | MVDTSLKLKDCVM | - | 426 |
| HLA-A*02:02 | WLDMVDTSLS | - | 441 | - |
| HLA-A*02:11 | FKLKDCVMYASAVV | - | 481 | - |
| HLA-A*11:01 | DTSLSGFKLK | DTSLKLK | 488 | - |
| HLA-B*35:01 | MVDTSLSGF | MVDTSL | 490 | - |
| HLA-A*31:01 | RIMTWLDMVDTSLSGFK | RIMTWLDMVDTSLK | - | 493 |
| HLA-B*15:03 | LSGFKLKDCVMY | LKLKDCVMY | 3466 | 22 |
| HLA-A*02:11 | LDMVDTSLSGFKL | LDMVDTSLKL | 16396 | 166 |
| HLA-A*02:02 | LDMVDTSLSGFKL | LDMVDTSLKL | 9971 | 106 |
| HLA-A*02:11 | SLSGFKLKDCV | SLKLKDCV | 122 | 9934 |
| HLA-A*02:01 | LDMVDTSLSGFKL | LDMVDTSLKL | 20579 | 311 |
| HLA-B*08:01 | SLSGFKLKDCVM | SLKLKDCVM | 929 | 36 |
| HLA-A*02:02 | SLSGFKLKDCV | SLKLKDCV | 408 | 10216 |
| HLA-C*05:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 8375 | 417 |
| HLA-B*15:01 | SLSGFKLKDCVMY | SLKLKDCVMY | 2952 | 158 |
| HLA-B*08:01 | TSLSGFKLKDCVM | TSLKLKDCVM | 4628 | 289 |
| HLA-B*15:01 | SLSGFKLKDCVM | SLKLKDCVM | 2654 | 197 |
| HLA-A*68:01 | DMVDTSLSGFK | DMVDTSLK | 366 | 4919 |
| HLA-A*02:11 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 1342 | 108 |
| HLA-C*08:02 | MVDTSLSGFKL | MVDTSLKL | 2134 | 174 |
| HLA-B*15:03 | SLSGFKLKDCVM | SLKLKDCVM | 3634 | 297 |
| HLA-A*02:01 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 2214 | 195 |
| HLA-A*03:02 | WLDMVDTSLSGFK | WLDMVDTSLK | 1756 | 156 |
| HLA-A*02:02 | LSGFKLKDCVMYA | LKLKDCVMYA | 305 | 28 |
| HLA-A*02:01 | LSGFKLKDCVMYA | LKLKDCVMYA | 1788 | 165 |
| HLA-A*02:11 | LSGFKLKDCVMYA | LKLKDCVMYA | 578 | 54 |
| HLA-B*15:03 | SLSGFKLKDCVMY | SLKLKDCVMY | 3626 | 349 |
| HLA-A*03:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 2900 | 287 |
| HLA-C*05:01 | MVDTSLSGFKL | MVDTSLKL | 1125 | 124 |
| HLA-A*11:01 | WLDMVDTSLSGFK | WLDMVDTSLK | 4020 | 492 |
| HLA-A*02:02 | WLDMVDTSLSGFKL | WLDMVDTSLKL | 788 | 116 |
| HLA-A*68:01 | DMVDTSLSGFKLK | DMVDTSLKLK | 1679 | 251 |
| HLA-A*02:01 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 203 | 853 |
| HLA-A*02:11 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 64 | 223 |
| HLA-A*02:02 | SLSGFKLKDCVMYA | SLKLKDCVMYA | 55 | 171 |
| HLA-A*29:02 | SLSGFKLKDCVMY | SLKLKDCVMY | 404 | 1192 |
| HLA-A*11:01 | MVDTSLSGFKLK | MVDTSLKLK | 143 | 50 |
| HLA-A*03:02 | MVDTSLSGFKLK | MVDTSLKLK | 312 | 127 |
| HLA-A*68:01 | MVDTSLSGFKLK | MVDTSLKLK | 602 | 246 |
| HLA-A*03:01 | MVDTSLSGFKLK | MVDTSLKLK | 441 | 190 |
V278I
Reference YMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ
||||||||||||||||||||||||| ||||||||||||
Mutated YMNSQGLLPPKNSIDAFKLNIKLLGIGGKPCIKVATVQ
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*32:01 | KLNIKLLGV | KLNIKLLGI | 1672 | 277 |
| HLA-A*02:01 | KLNIKLLGV | KLNIKLLGI | 35 | 211 |
| HLA-A*02:06 | KLNIKLLGV | KLNIKLLGI | 116 | 682 |
| HLA-A*02:11 | AFKLNIKLLGV | AFKLNIKLLGI | 356 | 1832 |
| HLA-A*02:01 | FKLNIKLLGV | FKLNIKLLGI | 41 | 191 |
| HLA-A*02:02 | KLNIKLLGV | KLNIKLLGI | 26 | 114 |
| HLA-A*02:06 | FKLNIKLLGV | FKLNIKLLGI | 161 | 667 |
| HLA-A*02:11 | FKLNIKLLGV | FKLNIKLLGI | 9 | 31 |
| HLA-A*02:11 | KLNIKLLGV | KLNIKLLGI | 5 | 17 |
| HLA-A*02:02 | FKLNIKLLGV | FKLNIKLLGI | 20 | 66 |
| HLA-A*02:11 | LLGVGGKPCI | LLGIGGKPCI | 998 | 404 |
NSP12 protein
P323L
Reference CVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHF
||||||||||||||||||||||||| |||||||||||||||||||||||||
Mutated CVNCLDDRCILHCANFNVLFSTVFPLTSFGPLVRKIFVDGVPFVVSTGYHF
| Allele | Reference peptide | Mutated peptide | Reference affinity (IC50, nM) | Mutated affinity (IC50, nM) |
|---|---|---|---|---|
| HLA-A*02:01 | FPPTSFGPLV | FPLTSFGPLV | 8999 | 21 |
| HLA-A*02:02 | FPPTSFGPLV | FPLTSFGPLV | 7237 | 18 |
| HLA-A*02:11 | FPPTSFGPLV | FPLTSFGPLV | 3405 | 9 |
| HLA-A*32:01 | VLFSTVFPP | VLFSTVFPL | 5415 | 27 |
| HLA-A*02:11 | LFSTVFPP | LFSTVFPL | 6706 | 35 |
| HLA-A*02:11 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 1827 | 12 |
| HLA-A*02:11 | NFNVLFSTVFPP | NFNVLFSTVFPL | 1779 | 13 |
| HLA-A*02:11 | NVLFSTVFPP | NVLFSTVFPL | 739 | 6 |
| HLA-C*03:03 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-C*03:04 | VLFSTVFPP | VLFSTVFPL | 28468 | 265 |
| HLA-A*02:11 | FNVLFSTVFPP | FNVLFSTVFPL | 413 | 4 |
| HLA-C*14:02 | VLFSTVFPP | VLFSTVFPL | 21407 | 220 |
| HLA-A*02:11 | FNVLFSTVFPPT | FNVLFSTVFPLT | 2618 | 27 |
| HLA-A*02:01 | LFSTVFPP | LFSTVFPL | 7475 | 81 |
| HLA-C*14:02 | LFSTVFPP | LFSTVFPL | 11979 | 138 |
| HLA-B*15:03 | VLFSTVFPP | VLFSTVFPL | 3115 | 36 |
| HLA-A*02:11 | VLFSTVFPP | VLFSTVFPL | 78 | 1 |
| HLA-C*03:02 | VLFSTVFPP | VLFSTVFPL | 20533 | 273 |
| HLA-A*02:01 | FNVLFSTVFPP | FNVLFSTVFPL | 656 | 9 |
| HLA-A*02:01 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 2569 | 37 |
| HLA-A*02:11 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 9448 | 142 |
| HLA-A*02:02 | NVLFSTVFPP | NVLFSTVFPL | 1088 | 17 |
| HLA-A*02:11 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 6143 | 96 |
| HLA-A*02:02 | FNVLFSTVFPP | FNVLFSTVFPL | 1014 | 16 |
| HLA-A*68:02 | NVLFSTVFPP | NVLFSTVFPL | 2213 | 37 |
| HLA-A*02:02 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 3126 | 53 |
| HLA-A*02:11 | PPTSFGPLV | PLTSFGPLV | 28329 | 483 |
| HLA-C*16:01 | VLFSTVFPP | VLFSTVFPL | 24733 | 432 |
| HLA-C*17:01 | VLFSTVFPP | VLFSTVFPL | 25087 | 439 |
| HLA-A*02:01 | FNVLFSTVFPPT | FNVLFSTVFPLT | 3765 | 66 |
| HLA-A*02:02 | LFSTVFPP | LFSTVFPL | 9311 | 167 |
| HLA-A*02:01 | NVLFSTVFPP | NVLFSTVFPL | 928 | 17 |
| HLA-A*02:02 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2542 | 47 |
| HLA-A*02:02 | VLFSTVFPP | VLFSTVFPL | 269 | 5 |
| HLA-A*02:01 | NFNVLFSTVFPP | NFNVLFSTVFPL | 2286 | 44 |
| HLA-A*02:06 | FPPTSFGPLV | FPLTSFGPLV | 3379 | 73 |
| HLA-A*02:01 | VLFSTVFPP | VLFSTVFPL | 179 | 4 |
| HLA-A*02:06 | FNVLFSTVFPP | FNVLFSTVFPL | 1609 | 37 |
| HLA-A*02:02 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4116 | 110 |
| HLA-A*02:11 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6695 | 180 |
| HLA-A*02:01 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 11451 | 311 |
| HLA-A*02:06 | VLFSTVFPP | VLFSTVFPL | 368 | 10 |
| HLA-A*02:11 | ANFNVLFSTVFPPT | ANFNVLFSTVFPLT | 14639 | 409 |
| HLA-A*02:01 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 12596 | 425 |
| HLA-A*68:01 | PTSFGPLVR | LTSFGPLVR | 908 | 36 |
| HLA-A*02:06 | NVLFSTVFPP | NVLFSTVFPL | 1611 | 66 |
| HLA-B*15:01 | VLFSTVFPP | VLFSTVFPL | 5731 | 243 |
| HLA-B*35:01 | FPPTSFGPL | FPLTSFGPL | 304 | 13 |
| HLA-B*53:01 | FPPTSFGPL | FPLTSFGPL | 3581 | 154 |
| HLA-C*01:02 | FPPTSFGPL | FPLTSFGPL | 217 | 4855 |
| HLA-A*02:01 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8519 | 384 |
| HLA-A*02:02 | FNVLFSTVFPPTS | FNVLFSTVFPLTS | 10920 | 497 |
| HLA-A*02:02 | FSTVFPPTSFGPLV | FSTVFPLTSFGPLV | 6958 | 322 |
| HLA-A*02:11 | VFPPTSFGPLV | VFPLTSFGPLV | 4242 | 199 |
| HLA-A*02:02 | CANFNVLFSTVFPP | CANFNVLFSTVFPL | 8183 | 391 |
| HLA-A*02:11 | NFNVLFSTVFPPT | NFNVLFSTVFPLT | 9111 | 472 |
| HLA-A*02:06 | ANFNVLFSTVFPP | ANFNVLFSTVFPL | 5191 | 304 |
| HLA-A*02:06 | NFNVLFSTVFPP | NFNVLFSTVFPL | 4220 | 274 |
| HLA-A*02:06 | FNVLFSTVFPPT | FNVLFSTVFPLT | 4868 | 388 |
| HLA-B*35:03 | FPPTSFGPL | FPLTSFGPL | 286 | 24 |
| HLA-A*03:01 | PTSFGPLVRK | LTSFGPLVRK | 554 | 47 |
| HLA-A*31:01 | PTSFGPLVR | LTSFGPLVR | 4402 | 390 |
| HLA-A*11:01 | PTSFGPLVR | LTSFGPLVR | 4068 | 379 |
| HLA-A*03:02 | PTSFGPLVRK | LTSFGPLVRK | 868 | 88 |
| HLA-B*07:02 | STVFPPTSFGPL | STVFPLTSFGPL | 777 | 79 |
| HLA-A*68:01 | PTSFGPLVRK | LTSFGPLVRK | 308 | 34 |
| HLA-B*07:02 | TVFPPTSFGPL | TVFPLTSFGPL | 1498 | 171 |
| HLA-A*02:11 | STVFPPTSFGPLV | STVFPLTSFGPLV | 2427 | 285 |
| HLA-A*02:11 | NVLFSTVFPPT | NVLFSTVFPLT | 1183 | 139 |
| HLA-A*33:03 | PTSFGPLVR | LTSFGPLVR | 3228 | 381 |
| HLA-A*26:01 | STVFPPTSF | STVFPLTSF | 786 | 95 |
| HLA-B*07:02 | VFPPTSFGPL | VFPLTSFGPL | 339 | 43 |
| HLA-A*02:01 | NVLFSTVFPPT | NVLFSTVFPLT | 2111 | 290 |
| HLA-B*42:01 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 1320 | 185 |
| HLA-B*42:01 | STVFPPTSFGPL | STVFPLTSFGPL | 738 | 111 |
| HLA-A*02:01 | VLFSTVFPPTSF | VLFSTVFPLTSF | 2225 | 342 |
| HLA-B*08:01 | FPPTSFGPL | FPLTSFGPL | 973 | 153 |
| HLA-B*42:01 | TVFPPTSFGPL | TVFPLTSFGPL | 843 | 142 |
| HLA-A*11:01 | PTSFGPLVRK | LTSFGPLVRK | 123 | 21 |
| HLA-B*42:01 | VFPPTSFGPL | VFPLTSFGPL | 274 | 47 |
| HLA-A*02:01 | VLFSTVFPPTS | VLFSTVFPLTS | 708 | 124 |
| HLA-B*07:02 | FSTVFPPTSFGPL | FSTVFPLTSFGPL | 2537 | 466 |
| HLA-B*07:02 | FPPTSFGPL | FPLTSFGPL | 42 | 8 |
| HLA-A*02:11 | VLFSTVFPPTSF | VLFSTVFPLTSF | 1019 | 195 |
| HLA-A*25:01 | STVFPPTSF | STVFPLTSF | 1533 | 296 |
| HLA-B*07:02 | FPPTSFGPLV | FPLTSFGPLV | 1131 | 236 |
| HLA-B*42:01 | FPPTSFGPL | FPLTSFGPL | 21 | 5 |
| HLA-A*26:01 | FSTVFPPTSF | FSTVFPLTSF | 1667 | 398 |
| HLA-B*51:01 | FPPTSFGPL | FPLTSFGPL | 1809 | 433 |
| HLA-A*02:11 | VLFSTVFPPTS | VLFSTVFPLTS | 309 | 74 |
| HLA-B*42:01 | FPPTSFGPLV | FPLTSFGPLV | 368 | 89 |
| HLA-A*02:02 | VLFSTVFPPTS | VLFSTVFPLTS | 1210 | 377 |
| HLA-C*03:02 | FPPTSFGPL | FPLTSFGPL | 960 | 313 |
| HLA-A*32:01 | STVFPPTSF | STVFPLTSF | 230 | 86 |
| HLA-B*58:01 | FSTVFPPTSF | FSTVFPLTSF | 88 | 33 |
| HLA-B*15:01 | STVFPPTSF | STVFPLTSF | 130 | 49 |
| HLA-A*02:01 | VLFSTVFPPT | VLFSTVFPLT | 61 | 23 |
| HLA-C*03:03 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-C*03:04 | FPPTSFGPL | FPLTSFGPL | 375 | 145 |
| HLA-A*02:02 | VLFSTVFPPT | VLFSTVFPLT | 113 | 44 |
| HLA-C*16:01 | FSTVFPPTS | FSTVFPLTS | 1127 | 460 |
| HLA-C*14:02 | LFSTVFPPTSF | LFSTVFPLTSF | 396 | 950 |
| HLA-C*01:02 | VFPPTSFGPL | VFPLTSFGPL | 429 | 997 |
| HLA-A*02:02 | TVFPPTSFGPLV | TVFPLTSFGPLV | 789 | 343 |
| HLA-A*68:02 | STVFPPTSFGPLV | STVFPLTSFGPLV | 169 | 74 |
| HLA-A*02:11 | VLFSTVFPPT | VLFSTVFPLT | 25 | 11 |
| HLA-A*03:01 | TVFPPTSFGPLVRK | TVFPLTSFGPLVRK | 92 | 198 |
| HLA-C*14:02 | VFPPTSFGPL | VFPLTSFGPL | 47 | 99 |
| HLA-A*23:01 | LFSTVFPPTSF | LFSTVFPLTSF | 370 | 179 |